ライフサイエンスコーパス: tRNA(Thr

1 activity that also generates mischarged Ser-tRNA(Thr).

2 onor to methylate t(6)A to form m(6)t(6)A in tRNA(Thr).

3 (C182 in Escherichia coli) to hydrolyze Ser-tRNA(Thr).

4 nt to 3' end, at conventional position 71 of tRNA(Thr).

5 ectively editing the misacylated species Ser-tRNA(Thr).

6 sition C(32) of the mt-tRNA(Ser(UCN)) and mt-tRNA(Thr).

7 or stem to specifically select tRNA(Gly) and tRNA(Thr).

8 t proofreading activity of ThrRS towards Ala-tRNAThr.

9 Thr and to lack editing activity against Ser-tRNAThr.

10 aRS mischarging alanine to G4:U69-containing tRNAThr.

11 These imply that the tRNA(Thr) A15951G mutation may have a potential modifier

12 -mediated repression, and focused on TRT2, a tRNA(Thr) adjacent to the STE6 alpha2 operator.

13 ting at position 32 of the anticodon loop of tRNA(Thr)(AGU) stimulates, but is not required for, the

14 We have shown that cytoplasmic tRNA(Thr)(AGU) undergoes two distinct editing events in

15 responsible for generating mitochondrial Thr-tRNAThr and clearing mischarged Ser-tRNAThr during mitoc

16 s ThrRS-cat was shown to synthesize both Thr-tRNAThr and Ser-tRNAThr and to lack editing activity aga

17 shown to synthesize both Thr-tRNAThr and Ser-tRNAThr and to lack editing activity against Ser-tRNAThr

18 In ThrRS, however, the 2'-OH of A76 of tRNA(Thr) and a conserved active-site histidine (His-309

19 charged at >80% levels, whereas tRNASer and tRNAThr are charged at lower levels.

20 ort that the mischargeable G4:U69-containing tRNAThr are strictly conserved in vertebrates and are ub

21 read by tRNA(Gln(TTG)), tRNA(Arg(CCG)), and tRNA(Thr(CGT)) These findings collectively reveal the pr

22 ced translation of mt-tRNA(Ser(UCN))- and mt-tRNA(Thr)-dependent codons through METTL8-mediated m(3)C

23 bosome stalling on mt-tRNA(Ser(UCN))- and mt-tRNA(Thr)-dependent codons.

24 rial Thr-tRNAThr and clearing mischarged Ser-tRNAThr during mitochondrial translation.

25 residue critical for editing, leading to Ser-tRNA(Thr) formation and protein mistranslation that impa

26 ond and final site of termination beyond the tRNAThr gene remains unclear.

27 Finally, we discovered that a tRNA(Thr) gene near HMR-I helped maintain silenced chrom

28 of auxiliary elements, such as HMR-I and the tRNA(Thr) gene, in enhancing the association of Sir sile

29 content of Escherichia coli tRNASer(VGA) and tRNAThr(GGU) as controls were measured as 2.03 and 2.84

30 analyses of E. coli tRNASer(VGA) and E. coli tRNAThr(GGU), unfractionated tRNA from E. coli and 23S r

31 f missense suppression by Cys-tRNA(Pro), Ser-tRNA(Thr), Glu-tRNA(Gln), and Asp-tRNA(Asn).

32 The adenine (A71) at this position of tRNA(Thr), highly conserved from bacteria to human mitoc

33 to five tRNAs, including human mitochondrial tRNAThr (hmtRNAThr).

34 Here we show that archaeal editing of Ser-tRNAThr is catalyzed by a domain unrelated to, and absen

35 In vivo, every inosine-containing tRNA(Thr) is also C to U edited at position 32.

36 in the anticodon loop of Trypanosoma brucei tRNA(Thr) is methylated to 3-methylcytosine (m(3)C) as a

37 showed that air oxidation increased the Ser-tRNA(Thr) level in the presence of elongation factor Tu.

38 Interestingly, although Ala-tRNAThr mischarging is not known to occur in bacteria, E

39 otein in trans to hydrolyze specifically Ser-tRNAThr, or it can be fused to ThrRS-cat to provide the

40 rogen peroxide oxidizes C182, leading to Ser-tRNA(Thr) production and mistranslation of threonine cod

41 installs m(3)C specifically on mitochondrial tRNA(Thr/Ser(UCN)) in mouse embryonic cortical neural st

42 le for the N(6)-methyl group of m(6)t(6)A in tRNA(Thr) specific for ACY codons.

43 reonyl-tRNA synthetases, responsible for Thr-tRNA(Thr) synthesis: one accurate and constitutively exp

44 intrinsic activity allows G4:U69-containing tRNAThr to emerge and be preserved in vertebrates to hav

45 an A3G mutation in Saccharomyces cerevisiae tRNA(Thr)UAG confers tRNA recognition by AgAlaRS.

46 e, early hypoxia increases wobble cmo(5)U in tRNA(Thr(UGU)), which parallels translation of transcrip

47 r the expression and processing of the genes tRNA(Thr)(UGU), tRNA(Leu)(UAA), and tRNA(Phe) (GAA) ther

48 cant reduction of the steady-state levels in tRNA(Thr) was observed in cells carrying both the A15951

49 in a critical error of L-Ala mischarged onto tRNA(Thr), which is proofread by Animalia-specific-tRNA

50 cine to threonine, which requires an unusual tRNA(Thr) with an enlarged 8-nt anticodon loop ( ).