ライフサイエンスコーパス: tRNA(Trp

1 ivated by uncharged tryptophan transfer RNA (tRNATrp).

2 riptionally and translationally by uncharged tRNA(Trp).

3 centrations of free tryptophan and uncharged tRNA(Trp).

4 rimer, whereas Rous sarcoma virus (RSV) uses tRNA(Trp).

5 in response to the accumulation of uncharged tRNA(Trp).

6 in response to the accumulation of uncharged tRNA(Trp).

7 ty to base pair with the T psi C loop of the tRNA(Trp).

8 ophan versus tyrosine, and aminoacylation of tRNA(Trp).

9 in response to the accumulation of uncharged tRNA(Trp).

10 is increased upon accumulation of uncharged tRNA(Trp).

11 ficant changes in the apparent KM for ATP or tRNA(Trp).

12 tive impact of six variables on the yield of tRNA(Trp).

13 e significantly correlated with the yield of tRNA(Trp).

14 2'-O methylations of C34 and U39 residues of tRNATrp.

15 om a 50/50% mixture of charged and uncharged tRNATrp.

16 re of human tryptophanyl-tRNA synthetase and tRNATrp.

17 slation of AT also is regulated by uncharged tRNATrp.

18 e operon encoding AT is induced by uncharged tRNATrp.

19 ween the 5' and 3' flanking sequences of the tRNA(Trp-1) gene from Arabidopsis to enhance its express

20 ate formation compared to wild-type for both tRNA(Trp)A-->C73 and.

21 In B. subtilis uncharged tRNA(Trp) accumulation activates transcription and trans

22 In E. coli uncharged tRNA(Trp) accumulation stalls the ribosome attempting tr

23 of phenylalanine, with the level of charged tRNA(Trp) acting as the crucial signal regulating AT pro

24 tion of the two nucleotides of exogenous pre-tRNA(Trp) added to an H. volcanii cell extract also occu

25 tures and the specificity helix, accelerates tRNA(Trp) aminoacylation with approximately 10-fold spec

26 ds tryptophan (Trp), ATP, and D. radiodurans tRNA(Trp) and catalyzes the formation of 5' adenyl-Trp a

27 and tRNA(Lys) were mainly mitochondrial; and tRNA(Trp) and tRNA(Val) were shared between the two comp

28 hanism in response to the level of uncharged tRNA(Trp), and that the presence of a trpS1 mutant allel

29 ng tRNASer(UCN), tRNASer(AGN), tRNAMet(AUA), tRNATrp, and tRNAPro genes occur in M. californianus mit

30 mbinant WRS-85D protein specifically charges tRNATrp, and WRS-85D is likely to be the only tryptophan

31 ypanosoma brucei by specifically editing the tRNA(Trp) anticodon to UCA, which can now decode the pre

32 res: only two transfer RNAs (tRNA(f-Met) and tRNA(Trp)) are encoded, and the cytochrome c oxidase sub

33 g and responding to tryptophan and uncharged tRNA(Trp) as regulatory signals.

34 onse to changes in the extent of charging of tRNA(Trp) as well as the availability of tryptophan.

35 Nuclease mapping studies of tRNATrp, as well as the viral RNA, indicate that the U5-

36 th the tetO inserted at position -1 (for the tRNA(Trp)AUC gene), or at positions -2, -6 and -10 (for

37 ing the Arabidopsis thaliana tRNA(Lys)AUC or tRNA(Trp)AUC suppressor tRNAs, and tRNA expression in ca

38 e regulated in vivo by the supply of charged tRNA(Trp) available to translate the second codon of the

39 When there is a deficiency of charged tRNA(Trp), B. subtilis forms an anti-TRAP protein, AT.

40 As the encoded mt-tRNA(Trp) cannot be folded into a totally orthodox secon

41 Here we show that a mutant tRNA(Trp) carrying a single substitution in its D-arm ac

42 ro analyses revealed that tRNA(Met(CAU)) and tRNA(Trp(CCA)) are substrates for Cm formation, tRNA(Gln

43 The tRNA(Trp)(CCA) can decode the standard UGG tryptophan co

44 n and extent of 5'- and 3'-end maturation of tRNA(Trp)(CCA), tRNA(Ile)(UAU), tRNA(Gln)(CUG), tRNA(Lys

45 y, we examined the roles of AT synthesis and tRNA(Trp) charging in mediating physiological responses

46 , reduces the availability of tryptophan for tRNA(Trp) charging.

47 indole acrylic acid, a specific inhibitor of tRNA(Trp) charging; cells deficient in expression of the

48 niformis is designed to allow a mild charged-tRNA(Trp) deficiency to expose the Shine-Dalgarno sequen

49 However, when there is a charged tRNA(Trp) deficiency, the translating ribosome presumabl

50 dditional tryptophan to overcome the charged tRNA(Trp) deficiency.

51 e we show that a readily denaturable, mutant tRNA(Trp) does not accumulate to normal levels in Escher

52 processed by endonuclease and ligase at the tRNATrp exon-intron boundaries.

53 -containing intron in the Haloferax volcanii tRNATrp gene abolishes RNA-guided 2'-O methylations of C

54 A partial tRNATrp gene, which contained only the anticodon stem-lo

55 sertion also contains a perfectly duplicated tRNA(Trp)gene, segments of several mitochondrial plasmid

56 sertion also contains a perfectly duplicated tRNA(Trp)gene, segments of several mitochondrial plasmid

57 e intron/exon splice site in H. volcanii pre-tRNATrp has been determined by NMR spectroscopy.

58 This showed that tRNA(Trp)identity has some effect on the ability of tRNA

59 The only apparent tRNA(trp) in these genomes completely overlaps the 5' en

60 of 2'-O-methylation for the H. volcanii pre-tRNA(Trp) in vitro by assembling methylation-competent b

61 Conversely, the accumulation of uncharged tRNA(Trp) induces synthesis of an anti-TRAP protein (AT)

62 In addition, accumulation of uncharged tRNA(Trp) induces synthesis of anti-TRAP (AT), which bin

63 accumulation of the excised H. volcanii pre-tRNA(Trp) intron in vivo.

64 ase factor 3 is disrupted, or when wild-type tRNATrP is overproduced.

65 at the cellular transfer RNA for tryptophan (tRNATrp) is predominantly uncharged.

66 One imported tRNA (tRNA(Trp)) is edited by a C to U modification at the fir

67 med with an AT-deficient mutant, the charged tRNA(Trp) level decreased even further.

68 ne to wild-type cultures reduced the charged tRNA(Trp) level from 80% to 40%; the charged level decre

69 findings suggest that, whenever the charged tRNA(Trp) level is sufficient to allow the ribosome tran

70 Changes in the charged tRNA(Trp) level observed during growth with added phenyl

71 han with phenylalanine increased the charged tRNA(Trp) level, implying that phenylalanine, when added

72 ype cultures gradually reduced their charged tRNA(Trp) level.

73 We suspected that readthrough of UGA by tRNATrp might be the reason for the partial function.

74 rom inhibition of TRAP function by uncharged tRNA(Trp) molecules or by increased synthesis of some ot

75 A) synthetase (TrpRS)-mutant opal suppressor tRNA(Trp) (mutRNA(UCA)(Trp)) pair was generated for use

76 e have studied the yield of Escherichia coli tRNA(Trp) obtained from in vitro T7 RNA polymerase trans

77 ox C/D RNPs positioned in either another pre-tRNA(Trp) or in the excised intron.

78 nary complexes containing Phe-tRNA(Phe), Trp-tRNA(Trp), or Leu-tRNA(LeuI).

79 enomic RNAs from mutant viruses in which the tRNA(Trp) PBS had been replaced with sequences homologou

80 bsence of these two enzymes large amounts of tRNA(Trp) precursor accumulate.

81 When a tRNATrp primer is annealed to wild-type RNAs in vitro, l

82 a virus (ASV) initiates from the 3' end of a tRNA(Trp) primer, which anneals near the 5' end of the R

83 Haloferax volcanii pre-tRNA(Trp) processing requires box C/D ribonucleoprotein

84 of the Haloferax volcanii, intron-containing tRNATrp RNA.

85 uctures of DusC complexes with tRNA(Phe) and tRNA(Trp) show that Dus subfamilies that selectively mod

86 ains complete genes for tRNA(f-Met), l-rRNA, tRNA(Trp), subunit 2 of cytochrome c oxidase (COII), sub

87 ported in vitro more efficiently than edited tRNA(Trp), suggesting the presence of importation determ

88 corresponding conditions yielded 6-fold more tRNA(Trp) than the initial conditions, confirming the pr

89 odification in the anticodon of the imported tRNA(Trp), thereby permitting the decoding of the UGA st

90 Tandem sensing of uncharged tRNATrp therefore regulates synthesis of AT, which in tu

91 assays were used to monitor the annealing of tRNA(Trp) to a PBS-containing RSV RNA.

92 the viral U5 RNA and the T psi C loop of the tRNA(Trp) (U5-T psi C).

93 th A36A37A38, only mt tRNAs tRNA(Ser)UGA and tRNA(Trp)UCA contained detectable i6A37.

94 We show that the mitochondrial tRNA(Trp) undergoes a specific C to U nucleotide modific

95 The effects of tRNA(Trp)variants on the aminoacylation reaction catalyz

96 5'-Extended precursor tRNA(Trp) was found to be unedited, which is consistent

97 T7-transcribed unedited tRNA(Trp) was imported in vitro more efficiently than ed

98 The anticodon loop of the Bacillus subtilis tRNA(Trp) was mutated to UCA, three positions in the D a

99 catalyzes the formation of 5' adenyl-Trp and tRNA(Trp), with approximately five times less activity t

100 regulatory signals, tryptophan and uncharged tRNA(Trp), yet they employ different mechanisms in regul