ライフサイエンスコーパス: tRNA(Trp)

1 2'-O methylations of C34 and U39 residues of tRNATrp.

2 om a 50/50% mixture of charged and uncharged tRNATrp.

3 re of human tryptophanyl-tRNA synthetase and tRNATrp.

4 slation of AT also is regulated by uncharged tRNATrp.

5 e operon encoding AT is induced by uncharged tRNATrp.

6 ivated by uncharged tryptophan transfer RNA (tRNATrp).

7 in response to the accumulation of uncharged tRNA(Trp).

8 e significantly correlated with the yield of tRNA(Trp).

9 is increased upon accumulation of uncharged tRNA(Trp).

10 riptionally and translationally by uncharged tRNA(Trp).

11 centrations of free tryptophan and uncharged tRNA(Trp).

12 in response to the accumulation of uncharged tRNA(Trp).

13 rimer, whereas Rous sarcoma virus (RSV) uses tRNA(Trp).

14 in response to the accumulation of uncharged tRNA(Trp).

15 ty to base pair with the T psi C loop of the tRNA(Trp).

16 ophan versus tyrosine, and aminoacylation of tRNA(Trp).

17 ficant changes in the apparent KM for ATP or tRNA(Trp).

18 tive impact of six variables on the yield of tRNA(Trp).

19 ween the 5' and 3' flanking sequences of the tRNA(Trp-1) gene from Arabidopsis to enhance its express

20 ate formation compared to wild-type for both tRNA(Trp)A-->C73 and.

21 In B. subtilis uncharged tRNA(Trp) accumulation activates transcription and trans

22 In E. coli uncharged tRNA(Trp) accumulation stalls the ribosome attempting tr

23 of phenylalanine, with the level of charged tRNA(Trp) acting as the crucial signal regulating AT pro

24 tion of the two nucleotides of exogenous pre-tRNA(Trp) added to an H. volcanii cell extract also occu

25 tures and the specificity helix, accelerates tRNA(Trp) aminoacylation with approximately 10-fold spec

26 ds tryptophan (Trp), ATP, and D. radiodurans tRNA(Trp) and catalyzes the formation of 5' adenyl-Trp a

27 and tRNA(Lys) were mainly mitochondrial; and tRNA(Trp) and tRNA(Val) were shared between the two comp

28 hanism in response to the level of uncharged tRNA(Trp), and that the presence of a trpS1 mutant allel

29 ng tRNASer(UCN), tRNASer(AGN), tRNAMet(AUA), tRNATrp, and tRNAPro genes occur in M. californianus mit

30 mbinant WRS-85D protein specifically charges tRNATrp, and WRS-85D is likely to be the only tryptophan

31 ypanosoma brucei by specifically editing the tRNA(Trp) anticodon to UCA, which can now decode the pre

32 res: only two transfer RNAs (tRNA(f-Met) and tRNA(Trp)) are encoded, and the cytochrome c oxidase sub

33 g and responding to tryptophan and uncharged tRNA(Trp) as regulatory signals.

34 onse to changes in the extent of charging of tRNA(Trp) as well as the availability of tryptophan.

35 Nuclease mapping studies of tRNATrp, as well as the viral RNA, indicate that the U5-

36 th the tetO inserted at position -1 (for the tRNA(Trp)AUC gene), or at positions -2, -6 and -10 (for

37 ing the Arabidopsis thaliana tRNA(Lys)AUC or tRNA(Trp)AUC suppressor tRNAs, and tRNA expression in ca

38 e regulated in vivo by the supply of charged tRNA(Trp) available to translate the second codon of the

39 When there is a deficiency of charged tRNA(Trp), B. subtilis forms an anti-TRAP protein, AT.

40 As the encoded mt-tRNA(Trp) cannot be folded into a totally orthodox secon

41 Here we show that a mutant tRNA(Trp) carrying a single substitution in its D-arm ac

42 The tRNA(Trp)(CCA) can decode the standard UGG tryptophan co

43 ath through shortening the anticodon stem of tRNA(Trp)(CCA) from five to four base pairs (bp).

44 n and extent of 5'- and 3'-end maturation of tRNA(Trp)(CCA), tRNA(Ile)(UAU), tRNA(Gln)(CUG), tRNA(Lys

45 ro analyses revealed that tRNA(Met(CAU)) and tRNA(Trp(CCA)) are substrates for Cm formation, tRNA(Gln

46 y, we examined the roles of AT synthesis and tRNA(Trp) charging in mediating physiological responses

47 , reduces the availability of tryptophan for tRNA(Trp) charging.

48 indole acrylic acid, a specific inhibitor of tRNA(Trp) charging; cells deficient in expression of the

49 niformis is designed to allow a mild charged-tRNA(Trp) deficiency to expose the Shine-Dalgarno sequen

50 However, when there is a charged tRNA(Trp) deficiency, the translating ribosome presumabl

51 dditional tryptophan to overcome the charged tRNA(Trp) deficiency.

52 e we show that a readily denaturable, mutant tRNA(Trp) does not accumulate to normal levels in Escher

53 processed by endonuclease and ligase at the tRNATrp exon-intron boundaries.

54 -containing intron in the Haloferax volcanii tRNATrp gene abolishes RNA-guided 2'-O methylations of C

55 A partial tRNATrp gene, which contained only the anticodon stem-lo

56 sertion also contains a perfectly duplicated tRNA(Trp)gene, segments of several mitochondrial plasmid

57 sertion also contains a perfectly duplicated tRNA(Trp)gene, segments of several mitochondrial plasmid

58 e intron/exon splice site in H. volcanii pre-tRNATrp has been determined by NMR spectroscopy.

59 This showed that tRNA(Trp)identity has some effect on the ability of tRNA

60 The presence of tRNA(Met) and tRNA(Trp) in both ceriantharian mitogenomes supports a c

61 orallia indicating a possible suppression of tRNA(Trp) in Octocorallia.

62 The only apparent tRNA(trp) in these genomes completely overlaps the 5' en

63 of 2'-O-methylation for the H. volcanii pre-tRNA(Trp) in vitro by assembling methylation-competent b

64 Conversely, the accumulation of uncharged tRNA(Trp) induces synthesis of an anti-TRAP protein (AT)

65 In addition, accumulation of uncharged tRNA(Trp) induces synthesis of anti-TRAP (AT), which bin

66 accumulation of the excised H. volcanii pre-tRNA(Trp) intron in vivo.

67 at the cellular transfer RNA for tryptophan (tRNATrp) is predominantly uncharged.

68 ase factor 3 is disrupted, or when wild-type tRNATrP is overproduced.

69 One imported tRNA (tRNA(Trp)) is edited by a C to U modification at the fir

70 med with an AT-deficient mutant, the charged tRNA(Trp) level decreased even further.

71 ne to wild-type cultures reduced the charged tRNA(Trp) level from 80% to 40%; the charged level decre

72 findings suggest that, whenever the charged tRNA(Trp) level is sufficient to allow the ribosome tran

73 Changes in the charged tRNA(Trp) level observed during growth with added phenyl

74 han with phenylalanine increased the charged tRNA(Trp) level, implying that phenylalanine, when added

75 ype cultures gradually reduced their charged tRNA(Trp) level.

76 We suspected that readthrough of UGA by tRNATrp might be the reason for the partial function.

77 rom inhibition of TRAP function by uncharged tRNA(Trp) molecules or by increased synthesis of some ot

78 A) synthetase (TrpRS)-mutant opal suppressor tRNA(Trp) (mutRNA(UCA)(Trp)) pair was generated for use

79 e have studied the yield of Escherichia coli tRNA(Trp) obtained from in vitro T7 RNA polymerase trans

80 ox C/D RNPs positioned in either another pre-tRNA(Trp) or in the excised intron.

81 nary complexes containing Phe-tRNA(Phe), Trp-tRNA(Trp), or Leu-tRNA(LeuI).

82 unctionally replacing the endogenous EcTrpRS-tRNA(Trp) pair in BL21(DE3) E. coli with an orthogonal c

83 the nonsense-suppressing engineered EcTrpRS-tRNA(Trp) pair was systematically optimized to significa

84 coli tryptophanyl-tRNA synthetase (EcTrpRS)-tRNA(Trp) pairs.

85 enomic RNAs from mutant viruses in which the tRNA(Trp) PBS had been replaced with sequences homologou

86 bsence of these two enzymes large amounts of tRNA(Trp) precursor accumulate.

87 a virus (ASV) initiates from the 3' end of a tRNA(Trp) primer, which anneals near the 5' end of the R

88 When a tRNATrp primer is annealed to wild-type RNAs in vitro, l

89 Haloferax volcanii pre-tRNA(Trp) processing requires box C/D ribonucleoprotein

90 The canonical 5-bp tRNA(Trp) recognizes UGG as dictated by the genetic code

91 of the Haloferax volcanii, intron-containing tRNATrp RNA.

92 uctures of DusC complexes with tRNA(Phe) and tRNA(Trp) show that Dus subfamilies that selectively mod

93 ains complete genes for tRNA(f-Met), l-rRNA, tRNA(Trp), subunit 2 of cytochrome c oxidase (COII), sub

94 ported in vitro more efficiently than edited tRNA(Trp), suggesting the presence of importation determ

95 corresponding conditions yielded 6-fold more tRNA(Trp) than the initial conditions, confirming the pr

96 odification in the anticodon of the imported tRNA(Trp), thereby permitting the decoding of the UGA st

97 Tandem sensing of uncharged tRNATrp therefore regulates synthesis of AT, which in tu

98 assays were used to monitor the annealing of tRNA(Trp) to a PBS-containing RSV RNA.

99 e then engineered release factor 2 (RF2) and tRNA(Trp) to mitigate native UGA recognition, translatio

100 the viral U5 RNA and the T psi C loop of the tRNA(Trp) (U5-T psi C).

101 th A36A37A38, only mt tRNAs tRNA(Ser)UGA and tRNA(Trp)UCA contained detectable i6A37.

102 We show that the mitochondrial tRNA(Trp) undergoes a specific C to U nucleotide modific

103 The effects of tRNA(Trp)variants on the aminoacylation reaction catalyz

104 5'-Extended precursor tRNA(Trp) was found to be unedited, which is consistent

105 T7-transcribed unedited tRNA(Trp) was imported in vitro more efficiently than ed

106 The anticodon loop of the Bacillus subtilis tRNA(Trp) was mutated to UCA, three positions in the D a

107 catalyzes the formation of 5' adenyl-Trp and tRNA(Trp), with approximately five times less activity t

108 regulatory signals, tryptophan and uncharged tRNA(Trp), yet they employ different mechanisms in regul