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1 2'-O methylations of C34 and U39 residues of tRNATrp.
2 om a 50/50% mixture of charged and uncharged tRNATrp.
3 re of human tryptophanyl-tRNA synthetase and tRNATrp.
4 slation of AT also is regulated by uncharged tRNATrp.
5 e operon encoding AT is induced by uncharged tRNATrp.
6 ivated by uncharged tryptophan transfer RNA (tRNATrp).
7 riptionally and translationally by uncharged tRNA(Trp).
8 centrations of free tryptophan and uncharged tRNA(Trp).
9 rimer, whereas Rous sarcoma virus (RSV) uses tRNA(Trp).
10 in response to the accumulation of uncharged tRNA(Trp).
11 in response to the accumulation of uncharged tRNA(Trp).
12 ty to base pair with the T psi C loop of the tRNA(Trp).
13 ophan versus tyrosine, and aminoacylation of tRNA(Trp).
14 in response to the accumulation of uncharged tRNA(Trp).
15 is increased upon accumulation of uncharged tRNA(Trp).
16 ficant changes in the apparent KM for ATP or tRNA(Trp).
17 tive impact of six variables on the yield of tRNA(Trp).
18 e significantly correlated with the yield of tRNA(Trp).
19 ween the 5' and 3' flanking sequences of the tRNA(Trp-1) gene from Arabidopsis to enhance its express
23 of phenylalanine, with the level of charged tRNA(Trp) acting as the crucial signal regulating AT pro
24 tion of the two nucleotides of exogenous pre-tRNA(Trp) added to an H. volcanii cell extract also occu
25 tures and the specificity helix, accelerates tRNA(Trp) aminoacylation with approximately 10-fold spec
26 ds tryptophan (Trp), ATP, and D. radiodurans tRNA(Trp) and catalyzes the formation of 5' adenyl-Trp a
27 and tRNA(Lys) were mainly mitochondrial; and tRNA(Trp) and tRNA(Val) were shared between the two comp
28 hanism in response to the level of uncharged tRNA(Trp), and that the presence of a trpS1 mutant allel
29 ng tRNASer(UCN), tRNASer(AGN), tRNAMet(AUA), tRNATrp, and tRNAPro genes occur in M. californianus mit
30 mbinant WRS-85D protein specifically charges tRNATrp, and WRS-85D is likely to be the only tryptophan
31 ypanosoma brucei by specifically editing the tRNA(Trp) anticodon to UCA, which can now decode the pre
32 res: only two transfer RNAs (tRNA(f-Met) and tRNA(Trp)) are encoded, and the cytochrome c oxidase sub
34 onse to changes in the extent of charging of tRNA(Trp) as well as the availability of tryptophan.
36 th the tetO inserted at position -1 (for the tRNA(Trp)AUC gene), or at positions -2, -6 and -10 (for
37 ing the Arabidopsis thaliana tRNA(Lys)AUC or tRNA(Trp)AUC suppressor tRNAs, and tRNA expression in ca
38 e regulated in vivo by the supply of charged tRNA(Trp) available to translate the second codon of the
43 n and extent of 5'- and 3'-end maturation of tRNA(Trp)(CCA), tRNA(Ile)(UAU), tRNA(Gln)(CUG), tRNA(Lys
44 ro analyses revealed that tRNA(Met(CAU)) and tRNA(Trp(CCA)) are substrates for Cm formation, tRNA(Gln
45 y, we examined the roles of AT synthesis and tRNA(Trp) charging in mediating physiological responses
47 indole acrylic acid, a specific inhibitor of tRNA(Trp) charging; cells deficient in expression of the
48 niformis is designed to allow a mild charged-tRNA(Trp) deficiency to expose the Shine-Dalgarno sequen
51 e we show that a readily denaturable, mutant tRNA(Trp) does not accumulate to normal levels in Escher
53 -containing intron in the Haloferax volcanii tRNATrp gene abolishes RNA-guided 2'-O methylations of C
55 sertion also contains a perfectly duplicated tRNA(Trp)gene, segments of several mitochondrial plasmid
56 sertion also contains a perfectly duplicated tRNA(Trp)gene, segments of several mitochondrial plasmid
60 of 2'-O-methylation for the H. volcanii pre-tRNA(Trp) in vitro by assembling methylation-competent b
61 Conversely, the accumulation of uncharged tRNA(Trp) induces synthesis of an anti-TRAP protein (AT)
68 ne to wild-type cultures reduced the charged tRNA(Trp) level from 80% to 40%; the charged level decre
69 findings suggest that, whenever the charged tRNA(Trp) level is sufficient to allow the ribosome tran
71 han with phenylalanine increased the charged tRNA(Trp) level, implying that phenylalanine, when added
74 rom inhibition of TRAP function by uncharged tRNA(Trp) molecules or by increased synthesis of some ot
75 A) synthetase (TrpRS)-mutant opal suppressor tRNA(Trp) (mutRNA(UCA)(Trp)) pair was generated for use
76 e have studied the yield of Escherichia coli tRNA(Trp) obtained from in vitro T7 RNA polymerase trans
79 enomic RNAs from mutant viruses in which the tRNA(Trp) PBS had been replaced with sequences homologou
81 a virus (ASV) initiates from the 3' end of a tRNA(Trp) primer, which anneals near the 5' end of the R
85 uctures of DusC complexes with tRNA(Phe) and tRNA(Trp) show that Dus subfamilies that selectively mod
86 ains complete genes for tRNA(f-Met), l-rRNA, tRNA(Trp), subunit 2 of cytochrome c oxidase (COII), sub
87 ported in vitro more efficiently than edited tRNA(Trp), suggesting the presence of importation determ
88 corresponding conditions yielded 6-fold more tRNA(Trp) than the initial conditions, confirming the pr
89 odification in the anticodon of the imported tRNA(Trp), thereby permitting the decoding of the UGA st
98 The anticodon loop of the Bacillus subtilis tRNA(Trp) was mutated to UCA, three positions in the D a
99 catalyzes the formation of 5' adenyl-Trp and tRNA(Trp), with approximately five times less activity t
100 regulatory signals, tryptophan and uncharged tRNA(Trp), yet they employ different mechanisms in regul
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