ライフサイエンスコーパス: tRNA(Tyr

1 motif is also important for interacting with tRNA(Tyr).

2 t of human TyrRS is regulated by its cognate tRNA(Tyr).

3 uclear distribution of TyrRS is regulated by tRNA(Tyr).

4 s signature feature of the anticodon loop in tRNA(Tyr).

5 and its subsequent transfer to the 3' end of tRNA(Tyr).

6 t which the tyrosyl moiety is transferred to tRNA(Tyr).

7 nding surface distinct from that which binds tRNA(Tyr).

8 r of both l- and d-tyrosine to the 3' end of tRNA(Tyr).

9 h the viral TLS was replaced with a cellular tRNA(Tyr).

10 with the D-/anticodon arm stacked helices of tRNA(Tyr).

11 e from the tyrosyl-adenylate intermediate to tRNA(Tyr).

12 e from the tyrosyl-adenylate intermediate to tRNA(Tyr).

13 oup I introns, in addition to aminoacylating tRNA(Tyr).

14 nding fold surface opposite that which binds tRNATyr.

15 -dependent genes (Pol III genes), tRNA(Leu), tRNA(Tyr), 5S rRNA and 7SL RNA.

16 Catalysis of tRNA(Tyr) aminoacylation by tyrosyl-tRNA synthetase can

17 Catalysis of tRNA(Tyr) aminoacylation by tyrosyl-tRNA synthetase invo

18 suggest that catalysis of the second step of tRNA(Tyr) aminoacylation involves stabilization of a tra

19 ylalanine (AzF) through the use of bacterial tRNA(Tyr) and a modified version of TyrRS, AzFRS, in Sch

20 onal proteins that both charge mitochondrial tRNA(Tyr) and act as splicing cofactors for autocatalyti

21 18 protein) both aminoacylates mitochondrial tRNA(Tyr) and acts as a structure-stabilizing splicing c

22 oncompetitive against the substrates preQ(1)-tRNA(Tyr) and AdoMet, respectively.

23 nal proteins that aminoacylate mitochondrial tRNA(Tyr) and are structure-stabilizing splicing cofacto

24 hat some C-terminal domain regions bind both tRNA(Tyr) and group I intron RNAs.

25 ious studies showed that CYT-18 has distinct tRNA(Tyr) and group I intron-binding sites, with the lat

26 ctional and both aminoacylates mitochondrial tRNA(Tyr) and promotes the splicing of mitochondrial gro

27 ce of the dihydrouridine (DHU) arm stem from tRNA(Tyr) and the use of CCG as an initiation codon for

28 d on the B.subtilis tyrS antiterminator with tRNA(Tyr) and tRNA acceptor stem models, using a gel shi

29 d analysis of structural differences between tRNA(Tyr) and tRNA species which interact inefficiently

30 endent and -independent pathways on the SUP4 tRNA(Tyr) and U6 snRNA (SNR6) genes, respectively, and f

31 on mechanism for the transfer of tyrosine to tRNA(Tyr), and suggest that catalysis of the second step

32 acylates human but not B. stearothermophilus tRNATyr, and vice versa, supporting the original hypothe

33 hermodynamic properties of Bacillus subtilis tRNA(Tyr) anticodon arms containing the natural base mod

34 us subtilis Our structure of QueG bound to a tRNA(Tyr) anticodon stem loop shows how this enzyme uses

35 s amino acids involved in the recognition of tRNATyr are not conserved.

36 ty of tRNACys at a cognate codon and that of tRNATyr at a near-cognate codon, suggesting that i6A37 p

37 al bi-ter kinetic mechanism in which preQ(1)-tRNA(Tyr) binds first followed by AdoMet, with product r

38 tRNA(Tyr) binds with a slightly (2.3-fold) lower affinit

39 merase III produces not only full-length pre-tRNATyr but also short oligonucleotides, indicating that

40 methionine was uncompetitive versus preQ(1)-tRNA(Tyr), but noncompetitive against AdoMet.

41 ity and TyrRS activity with the N. crassa mt tRNA(Tyr), but not for TyrRS activity with Escherichia c

42 SU and ND1 introns with that in N. crassa mt tRNA(Tyr) by constructing three-dimensional models based

43 turnover kinetics for the aminoacylation of tRNA(Tyr) by D-tyrosine were monitored using stopped-flo

44 l for Val-tRNA(Glu) to -8.1 kcal/mol for Val-tRNA(Tyr), clearly establishing EF-Tu*GTP as a sequence-

45 of the tRNA in a Thermus thermophilus TyrRS/tRNA(Tyr) cocrystal structure.

46 ependently, interactions of RNase P with pre-tRNA(Tyr) containing either the 5' leader, the 3' traile

47 D-Tyr-tRNA(Tyr) deacylase is an editing enzyme that removes d-

48 K230 and K233 during the initial binding of tRNA(Tyr) (DeltaDeltaG(int) = -0.74 kcal/mol).

49 pectation was confirmed by RNAi knockdown of tRNA(Tyr) expression, which led to robust nuclear import

50 As a notable exception, tRNA(Tyr) from Escherichia coli was not immunostimulator

51 epletion of nuclear Mod5p-II does not affect tRNATyr function.

52 /-2 on the nontranscribed strand of the SUP4 tRNATyr gene along with an [alpha-32P]dNMP by primer ext

53 deoxyuridine were incorporated into the SUP4 tRNATyr gene at bp -3/-2 or +11.

54 generated sequence substitutions in the SUP4 tRNATyr gene TFIIIB binding site.

55 TFIIIB with the region upstream of the SUP4 tRNATyr gene was extensively probed by use of photoreact

56 merase III transcription complex on the SUP4 tRNATyr gene was probed at distances of approximately 10

57 During transcription of the SUP4 tRNATyr gene, RNA polymerase III produces not only full-

58 lacking the arrest sites present in the SUP4 tRNATyr gene.

59 er dwell time at pause sites within the SUP4 tRNA(Tyr) gene.

60 corporated at specific sites within the SUP4 tRNA(Tyr) gene.

61 a brucei, the single intron-containing tRNA (tRNA(Tyr)GUA) is responsible for decoding all tyrosine c

62 cal editing events, within the intron of pre-tRNA(Tyr)GUA, involving guanosine-to-adenosine transitio

63 ation of tyrosine and subsequent transfer to tRNA(Tyr) has been determined using single turnover kine

64 not for TyrRS activity with Escherichia coli tRNA(Tyr), implying a somewhat different mode of recogni

65 an overexpression system was constructed for tRNA(Tyr) in an E. coli queA deletion mutant to allow fo

66 clear import of TyrRS directly controlled by tRNA(Tyr) in higher organisms, the NLS of lower eukaryot

67 , 5'-unphosphorylated in vitro transcript of tRNA(Tyr) induced IFN-alpha, thus revealing posttranscri

68 Aminoacylation of tRNA(Tyr) involves two steps: (1) tyrosine activation to

69 The SUP4 tRNA(Tyr) locus in Saccharomyces cerevisiae has been stu

70 The failure in tRNA(Tyr) metabolism impaired mitochondrial translation,

71 haracterized substrate-enzyme conjugate [pre-tRNA(Tyr)-Methanocaldococcus jannaschii (Mja) RPR] to in

72 ld decrease in the self-cleavage rate of pre-tRNA(Tyr)-MjaDeltaU RPR compared to the wild type, and t

73 a large shift on its flexible linker to bind tRNA(Tyr) or the intron RNA on either side of the cataly

74 To test the hypothesis that tRNATyr recognition differs between bacterial and human

75 d to display a dramatically reduced level of tRNA(Tyr)su3+ suppressor activity.

76 ns affects either the initial binding of the tRNA(Tyr) substrate or the stability of the transition s

77 -bound tyrosyl-adenylate intermediate to the tRNA(Tyr) substrate).

78 e does not affect the initial binding of the tRNA(Tyr) substrate, it destabilizes the transition stat

79 inoacylate the amber suppressor derived from tRNATyr (supF) with glutamine were cocrystallized with w

80 dase) and essential tyrS (encoding aminoacyl-tRNA(Tyr) synthetase) in a multifunctional operon.

81 Consistently, overexpression of a mutant pre-tRNA(Tyr) that cannot be processed by RNase P had a Gcd(

82 ed from tRNA(Glu), tRNA(Asp), tRNA(Gly), and tRNA(Tyr) that, upon induction, suppress the stability o

83 (G) in tRNA(His), tRNA(Asp), tRNA(Asn), and tRNA(Tyr); this changes the optimal binding from codons

84 otif is important for the initial binding of tRNA(Tyr) to tyrosyl-tRNA synthetase, it does not play a

85 motif plays a role in the initial binding of tRNA(Tyr) to tyrosyl-tRNA synthetase.

86 ther contains 27 tandem repeats of tRNA(Tyr)-tRNA(Tyr)-tRNA(Ser) genes.

87 and the other contains 27 tandem repeats of tRNA(Tyr)-tRNA(Tyr)-tRNA(Ser) genes.

88 approach based on catalytic DNA, a panel of tRNA(Tyr) variants featuring differential modification p

89 Binding of the antiterminator RNA to tRNA(Tyr) was dependent on complimentarity with the acce

90 Inhibition by the tRNA product (oQ-tRNA(Tyr)) was competitive and noncompetitive against th

91 synthetase to catalyze the aminoacylation of tRNA(Tyr), we have expressed each of these variants as r

92 cylated efficiency and steady-state level of tRNA(Tyr) were markedly decreased in the cell lines deri

93 centration and uncompetitive against preQ(1)-tRNA(Tyr) when AdoMet was saturating.

94 physically with tRNAs and in particular with tRNA(Tyr), which are present in the modifier and with th

95 o acid pools, suggesting that mischarging of tRNA(Tyr) with noncognate Phe by tyrosyl-tRNA synthetase

96 ynthetase where contacts of Escherichia coli tRNA(Tyr) with the alpha2 dimeric protein have been loca

97 and noncompetitive patterns against preQ(1)-tRNA(Tyr), with K(i) values of 133 +/- 18 and 4.6 +/- 0.