ライフサイエンスコーパス: tRNA(Val

1 on of the deacylated form of the abnormal mt-tRNA(Val).

2 xtremely low (<1%) steady-state levels of mt-tRNA(Val).

3 ics and flexibility of native and unmodified tRNA(val).

4 es are very similar in native and unmodified tRNA(val).

5 that is dependent on the presence of cognate tRNA(Val).

6 on that prevents accumulation of misacylated tRNA(Val).

7 (1) and 6 U-N(3) (15)N nuclei in a sample of tRNA(Val).

8 s bound with affinity similar to that of Val-tRNAVal.

9 R spectroscopy of 5-fluorouracil-substituted tRNAVal.

10 y intercalates into the acceptor stem of the tRNAVal.

11 -tRNAMetm (CAU anticodon) and mischarged Met-tRNAVal-1 (CAU anticodon) are substrates for the L/F-tra

12 aminoacylation of alpha-casein, whereas Val-tRNAVal-1 (UAC), Val-tRNAMetm (UAC), and Arg-tRNAMetm (C

13 alyl-tRNA synthetase does not edit wild-type tRNA(Val)(A76) mischarged with isoleucine, presumably be

14 emonstrates rapid degradation of preexisting tRNA(Val(AAC)) accompanied by its de-aminoacylation.

15 t both degradation and deacylation of mature tRNA(Val(AAC)) in a trm8-Delta trm4-Delta strain and res

16 3' exonucleases Rat1 and Xrn1 degrade mature tRNA(Val(AAC)) in yeast mutants lacking m(7)G and m(5)C,

17 healthy growth at 37 degrees C, hypomodified tRNA(Val(AAC)) is at least partially functional and stru

18 rapid tRNA decay (RTD) pathway, since mature tRNA(Val(AAC)) lacking 7-methylguanosine and 5-methylcyt

19 Multiple copies of tRNA(Val(AAC)) suppress the trm8-delta trm4-delta growth

20 e double mutants indicates reduced levels of tRNA(Val(AAC)), consistent with a role of the correspond

21 ates between base pairs corresponding to the tRNAVal acceptor stem in this molecule.

22 ix, and the 5' side of the anticodon stem of tRNAVal against cleavage by double- and single-strand-sp

23 ding of ethidium bromide to Escherichia coli tRNAVal and an RNA minihelix based on the acceptor stem

24 demonstrated for high-resolution analysis of tRNAval and its mutants, including a three-nucleotide de

25 ed the interactions between Escherichia coli tRNAVal and valyl-tRNA synthetase (ValRS) by enzymatic f

26 Mutational analysis of Escherichia coli tRNA(Val) and identity switch experiments with non-cogna

27 ts in reduced levels of mature tRNA(Asp) and tRNA(Val) and that altered protein production during dev

28 r tRNA(Gly) and tRNA(Leu), the mitochondrial tRNA(Val) and tRNA(Pro)) were strongly associated with t

29 ular tRNA precursor substrate for tRNA(Ala), tRNA(Val), and tRNA(His).

30 pressors reported here, one (JSN2) encodes a tRNAVal, and the other (JSN3) is an antimorphic allele o

31 the human mitoribosome when levels of the mt-tRNA(Val) are depleted.

32 ted mutant tRNAs as well as 3'-end-truncated tRNA(Val) are mixed noncompetitive inhibitors of the ami

33 d strikingly, when steady-state levels of mt-tRNA(Val) are reduced, human mitoribosome biogenesis dis

34 ty determinants to productive recognition of tRNA(Val) at the aminoacylation and editing sites, and b

35 nstrate that the dynamics and flexibility of tRNA(val), but not the local or global structure, are si

36 While valylation of the wild-type tRNA(Val) by the class I ValRS was strictly dependent on

37 e)(UAU), tRNA(Gln)(CUG), tRNA(Lys)(UUU), and tRNA(Val)(CAC).

38 itoribosomes have been shown to integrate mt-tRNA(Val) compared with the porcine use of mt-tRNA(Phe)

39 estore steady-state levels of the mutated mt-tRNA(Val), consistent with an increased stability of the

40 The amino acid acceptor arm of tRNAVal contains no other synthetase recognition nucleot

41 oacylation and editing sites, and by probing tRNA(Val) for editing determinants that are distinct fro

42 e positions in the 5' flanking region of the tRNA(Val) gene were repaired more efficiently than the g

43 g the anticodon stem of tRNAPhe with that of tRNAVal, however, converts the tRNA into a good substrat

44 Evidently, a purine at position 76 of tRNA(Val) is essential for translational editing by ValR

45 s results have shown that the 3'-terminus of tRNA(Val) is recognized differently at the two sites.

46 , a monomeric enzyme, may bind more than one tRNA(Val) molecule.

47 As a result 3'-end tRNA(Val) mutants, particularly those with 3'-terminal p

48 the generalized decrease in steady-state mt-tRNA(Val) observed in the homoplasmic 1624C>T-cell lines

49 2'-hydroxyl group, that of the U73 mutant of tRNA(Val) occurred at either the 2' or 3'-hydroxyl group

50 Our data demonstrate that only mt-tRNA(Val) or mt-tRNA(Phe) are found in the mitoribosomes

51 2 by overexpressing a mutant tRNA(AAC)(Val) (tRNA(Val*)) or the RNA component of RNase MRP encoded by

52 plification was performed targeting 16S rRNA/tRNA(val) region having an amplicon size of 530bp using

53 genes, trnD and trnV, encoding tRNA(Asp) and tRNA(Val), respectively, composing an operon at the attB

54 tase (ValRS) deacylate Val-tRNA(Ile) and Thr-tRNA(Val), respectively.

55 Similar results were obtained using pre-tRNA(Val)s containing a 5' leader of various lengths.

56 The mutant tRNA(Val*) showed nuclear accumulation in otherwise wild

57 able to hydrolytically deacylate misacylated tRNA(Val) terminating in 3'-pyrimidines but does deacyla

58 3'-pyrimidines but does deacylate mischarged tRNA(Val) terminating in adenosine or guanosine.

59 red to human tRNA gene promoters (tRNA(Met), tRNA(Val)), the human small nuclear RNA U6 gene (U6) and

60 itment of mitochondrial valine transfer RNA (tRNA(Val)) to play an integral structural role, and chan

61 sequences differ significantly from that of tRNAVal, to efficient valine acceptors.

62 sis of the aminoacylation kinetics of mutant tRNAVal transcripts.

63 he ends of the anticodon- and T-stems in the tRNAVal.ValRS complex is indicative of enzyme-induced co

64 ase.19F NMR also shows that formation of the tRNAVal-valyl-tRNA synthetase complex does not disrupt t

65 elix based on the acceptor stem and T-arm of tRNAVal was investigated by 19F and 1H NMR spectroscopy

66 tion of the nucleotide U73 of tRNA(Cys) into tRNA(Val) was found to confer the flexibility.

67 of the acceptor-TpsiC helix of tRNA(Ile) or tRNA(Val) were aminoacylated by cognate synthetases sele

68 st and steady-state levels of the mutated mt-tRNA(Val) were greater than in the biopsy material, but

69 were mainly mitochondrial; and tRNA(Trp) and tRNA(Val) were shared between the two compartments.

70 e structure and dynamics of Escherichia coli tRNA(val) were studied by NMR spectroscopy.

71 acylation of wild-type and 3'-end mutants of tRNA(Val) with isoleucine.

72 periments to characterize the interaction of tRNA(Val) with the enzyme provide evidence for two tRNA

73 to the recently refined structural model of tRNA(Val) yields the magnitude, asymmetry, and orientati