ライフサイエンスコーパス: tRNA(Val)

7 that is dependent on the presence of cognate tRNA(Val).

8 Mutational analysis of Escherichia coli tRNA(Val) and identity switch experiments with non-cogna

9 ding of ethidium bromide to Escherichia coli tRNAVal and an RNA minihelix based on the acceptor stem

10 ed the interactions between Escherichia coli tRNAVal and valyl-tRNA synthetase (ValRS) by enzymatic f

11 As a result 3'-end tRNA(Val) mutants, particularly those with 3'-terminal p

12 healthy growth at 37 degrees C, hypomodified tRNA(Val(AAC)) is at least partially functional and stru

13 tion of the nucleotide U73 of tRNA(Cys) into tRNA(Val) was found to confer the flexibility.

14 3' exonucleases Rat1 and Xrn1 degrade mature tRNA(Val(AAC)) in yeast mutants lacking m(7)G and m(5)C,

15 t both degradation and deacylation of mature tRNA(Val(AAC)) in a trm8-Delta trm4-Delta strain and res

16 rapid tRNA decay (RTD) pathway, since mature tRNA(Val(AAC)) lacking 7-methylguanosine and 5-methylcyt

17 red to human tRNA gene promoters (tRNA(Met), tRNA(Val)), the human small nuclear RNA U6 gene (U6) and

18 -tRNAMetm (CAU anticodon) and mischarged Met-tRNAVal-1 (CAU anticodon) are substrates for the L/F-tra

19 able to hydrolytically deacylate misacylated tRNA(Val) terminating in 3'-pyrimidines but does deacyla

20 on that prevents accumulation of misacylated tRNA(Val).

21 3'-pyrimidines but does deacylate mischarged tRNA(Val) terminating in adenosine or guanosine.

22 r tRNA(Gly) and tRNA(Leu), the mitochondrial tRNA(Val) and tRNA(Pro)) were strongly associated with t

23 on of the deacylated form of the abnormal mt-tRNA(Val).

24 itoribosomes have been shown to integrate mt-tRNA(Val) compared with the porcine use of mt-tRNA(Phe)

25 st and steady-state levels of the mutated mt-tRNA(Val) were greater than in the biopsy material, but

26 estore steady-state levels of the mutated mt-tRNA(Val), consistent with an increased stability of the

27 d strikingly, when steady-state levels of mt-tRNA(Val) are reduced, human mitoribosome biogenesis dis

28 xtremely low (<1%) steady-state levels of mt-tRNA(Val).

29 Our data demonstrate that only mt-tRNA(Val) or mt-tRNA(Phe) are found in the mitoribosomes

30 the generalized decrease in steady-state mt-tRNA(Val) observed in the homoplasmic 1624C>T-cell lines

31 the human mitoribosome when levels of the mt-tRNA(Val) are depleted.

32 The mutant tRNA(Val*) showed nuclear accumulation in otherwise wild

33 sis of the aminoacylation kinetics of mutant tRNAVal transcripts.

34 Evidently, a purine at position 76 of tRNA(Val) is essential for translational editing by ValR

35 periments to characterize the interaction of tRNA(Val) with the enzyme provide evidence for two tRNA

36 to the recently refined structural model of tRNA(Val) yields the magnitude, asymmetry, and orientati

37 2'-hydroxyl group, that of the U73 mutant of tRNA(Val) occurred at either the 2' or 3'-hydroxyl group

38 acylation of wild-type and 3'-end mutants of tRNA(Val) with isoleucine.

39 ty determinants to productive recognition of tRNA(Val) at the aminoacylation and editing sites, and b

40 (1) and 6 U-N(3) (15)N nuclei in a sample of tRNA(Val).

41 s results have shown that the 3'-terminus of tRNA(Val) is recognized differently at the two sites.

42 Multiple copies of tRNA(Val(AAC)) suppress the trm8-delta trm4-delta growth

43 e double mutants indicates reduced levels of tRNA(Val(AAC)), consistent with a role of the correspond

44 The amino acid acceptor arm of tRNAVal contains no other synthetase recognition nucleot

45 elix based on the acceptor stem and T-arm of tRNAVal was investigated by 19F and 1H NMR spectroscopy

46 ix, and the 5' side of the anticodon stem of tRNAVal against cleavage by double- and single-strand-sp

47 g the anticodon stem of tRNAPhe with that of tRNAVal, however, converts the tRNA into a good substrat

48 sequences differ significantly from that of tRNAVal, to efficient valine acceptors.

49 , a monomeric enzyme, may bind more than one tRNA(Val) molecule.

50 of the acceptor-TpsiC helix of tRNA(Ile) or tRNA(Val) were aminoacylated by cognate synthetases sele

51 Similar results were obtained using pre-tRNA(Val)s containing a 5' leader of various lengths.

52 emonstrates rapid degradation of preexisting tRNA(Val(AAC)) accompanied by its de-aminoacylation.

53 oacylation and editing sites, and by probing tRNA(Val) for editing determinants that are distinct fro

54 itment of mitochondrial valine transfer RNA (tRNA(Val)) to play an integral structural role, and chan

55 R spectroscopy of 5-fluorouracil-substituted tRNAVal.

56 e positions in the 5' flanking region of the tRNA(Val) gene were repaired more efficiently than the g

57 he ends of the anticodon- and T-stems in the tRNAVal.ValRS complex is indicative of enzyme-induced co

58 ase.19F NMR also shows that formation of the tRNAVal-valyl-tRNA synthetase complex does not disrupt t

59 y intercalates into the acceptor stem of the tRNAVal.

60 ates between base pairs corresponding to the tRNAVal acceptor stem in this molecule.

61 tase (ValRS) deacylate Val-tRNA(Ile) and Thr-tRNA(Val), respectively.

62 ted mutant tRNAs as well as 3'-end-truncated tRNA(Val) are mixed noncompetitive inhibitors of the ami

63 While valylation of the wild-type tRNA(Val) by the class I ValRS was strictly dependent on

64 alyl-tRNA synthetase does not edit wild-type tRNA(Val)(A76) mischarged with isoleucine, presumably be

65 2 by overexpressing a mutant tRNA(AAC)(Val) (tRNA(Val*)) or the RNA component of RNase MRP encoded by

66 s bound with affinity similar to that of Val-tRNAVal.

67 aminoacylation of alpha-casein, whereas Val-tRNAVal-1 (UAC), Val-tRNAMetm (UAC), and Arg-tRNAMetm (C