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1 eting the ghrelin receptor using fluorine-18 tagged entities would allow localization and visualizati
3 , live-cell imaging of end-binding protein 3 tagged with EGFP (EB3-GFP) in primary external granule l
4 including single tags 3xFLAG, 3xHA, 3xStrep-tag II, 18xHis, 13xMyc, and dual tags, all of which were
5 We performed an association study using 6 tagging SNPs, (rs860170, rs978739, rs1357949, rs1525489,
6 anslational modification that functions as a tag for a diverse array of cellular processes, wherein d
11 o unravel this puzzle, we employed a poly(A) tag sequencing protocol and uncovered a different poly(A
13 -based biorthogonal non-canonical amino acid tagging (BONCAT) - for studying the activity and biogeoc
14 adapt bioorthogonal noncanonical amino acid tagging (BONCAT) to interrogate protein synthesis in veg
18 proteomic technique, isotope-coded affinity tag labeling, to quantify the extent of NO2(-)-dependent
19 (S-nitrosothiol redox isotope-coded affinity tag), we found that exposure to NO2(-) under normoxic co
20 ondria expressing simple artificial affinity tags, which can also be employed to divert endogenous tr
22 ad to applications such as sensors, affinity tags, immobilization techniques, and protein-based mater
23 suggested the feasibility of these affinity tags in Streptomyces, which will be widely employed to e
24 of integrative vectors with various affinity tags, including single tags 3xFLAG, 3xHA, 3xStrep-tag II
25 combinant nature allows fusion with affinity tags or reporter enzymes as well as efficient maleimide
30 were expressed in Xenopus laevis oocytes and tagged with environmentally sensitive fluorescent rhodam
33 tem annotation and k-mer classification, and tags new genomes as having signatures that indicate susc
34 s study area, telemetry data from 54 animals tagged between 2003 and 2015 were used to produce densit
35 ARL15, PIK3R1, EYA4, ASTN2, and EPB41L3) are tagged by common SNPs unique to the 1000 Genomes referen
40 with genetically encoded lanthanide-binding tags (LBTs) inserted at the extracellular end of the S4
41 ls are exposed to increased irradiance, both tagged Psb28 proteins additionally associate with oligom
50 el amine-reactive mass defect-based chemical tag, dimethyl pyrimidinyl ornithine (DiPyrO), that is co
52 emical labeling, allowing efficient chemical tagging with a reduced excess of labeling reagent withou
53 can exchange d-amino acids bearing chemical tags or affinity handles into peptidoglycan precursors,
54 approach using genetically encoded chemical tags CLIP, SNAP, Halo, and TMP for tissue labeling; this
55 application, we mutated CE7 to create a CL7 tag, which retained the full binding affinity to Im7 but
57 ensional (2D) boron polymorphs, collectively tagged borophene, are all metallic with high free charge
60 fic, as adaptors with artificial degradation tags were not protected even though cargo binding is una
61 Optical pulse-chase experiments with Dendra2-tagged aSyn versions indicated that the E46K mutation do
67 scuss the design and synthesis of the DiPyrO tag, characterize its effect on labeled proteome analysi
69 s of DNA-encoded bioactive compounds and DNA-tagged human kinases to identify ligand:protein binding
71 ution and gas phase measurements on a doubly tagged tripeptide shows that dimer-induced fluorescence
72 HA, 3xStrep-tag II, 18xHis, 13xMyc, and dual tags, all of which were driven from a strong constitutiv
75 strate the sensitivity and specificity of EC-tagging by obtaining cell type-specific gene expression
80 rks wild-type cells with the endogenous EGFP-tagged protein, whereas mutant cells are marked with mCh
82 ised by fusing the protein with one of eight tags: hexahistidine (His6), thioredoxin (Trx), small ubi
83 ayed individual behaviour and electronically tagged hundreds of fish (roach, Rutilus rutilus) before
86 chromatin interaction analysis by paired-end tag (ChIA-PET) sequencing data, we used CRISPR-Cas9 gene
88 gents, we affinity-purified 785 endogenously tagged CEPs and identified stably associated polypeptide
89 P system consists of a self-labelling enzyme tag, which catalyses the covalent linking of exogenously
91 otype in the sporozoite arising from epitope tagging of the endogenous protein) as a key regulator fo
93 ssing two Cdc20 genes with different epitope tags, we show by co-immunoprecipitation that this is tru
94 factor genes; (3) simple knock-in of epitope tags and fluorescent reporters (e.g. Sox2-V5 and Sox2-mC
95 astoid cell lines (LCLs), expressing epitope-tagged EBNA3A, EBNA3B or EBNA3C from EBV-recombinants, r
97 Co-immunoprecipitation studies with epitope-tagged PP1c and Gbeta1 revealed that Gbeta1 interacts wi
101 ntify an optimum size range that facilitates tagging and tracking of thousands of cells simultaneousl
103 singly, despite the presence of the large Fc tag, Fc-d E1E2 formed heterodimers similar to those form
104 Affinity purification and removal of the Fc tag from E1E2 resulted in an antigen with a nearly ident
105 ity of affinity resins for capture of the Fc-tagged rE1E2, we anticipate that our method will provide
106 le-stranded DNA, and we have identified five tags that react orthogonally with distinct DNA sequences
107 alysis in cultured cells overexpressing FLAG-tagged wild-type or mutant TUBB4B as well as in primary
111 developed a fast ligand identification (FLI) tag that can be attached to any alkyne-containing ligand
112 cence polarization analysis of a fluorescein-tagged peptide corresponding to pol beta residues 2-13.
113 Here we report the use of a fluorescein-tagged peroxisomal targeting sequence peptide (F-PTS1, a
114 gene expression, as well as for fluorescence tagging of chromosomal regions and individual mRNAs to t
119 properties and conjugation with fluorescent tags are demonstrated to highlight the potential applica
120 out need to stain, fix, lyse, or fluorescent-tags are desirable in basic stem cell technology and the
123 on calcium-mediated binding of fluorescently tagged long oligogalacturonides (OGs) with endogenous de
124 myosin motors to forcibly pull fluorescently tagged protein complexes along filopodial actin filament
125 ntional brightness analysis to fluorescently tagged proteins within the NE exhibited an unusual conce
127 se results provide an empirical baseline for tagging experiments, life histories extrapolated from ot
128 nalogous anterograde transsynaptic tools for tagging postsynaptically targeted neurons remain under d
131 ed as genetically encoded fluorescent fusion tags, and there is an increasing interest in engineering
132 aphy with iodine-filled lumen and gadolinium-tagged polyps may enable ready differentiation between p
134 se 2 (APEX2) as a broadly applicable genetic tag that generates EM contrast on a specific protein or
141 y expressing green fluorescent protein (GFP)-tagged wild-type La (GFP-La(WT)) grow faster than cells
143 vesicles (DCVs) that were filled with a GFP-tagged neuropeptide in the Drosophila nervous system.
144 vo Knock-in mice of both sexes bearing a GFP-tagged phospho-dead S193A allele on a null background (A
145 function in body tissues, we generated a GFP-tagged-cry transgene that rescues light-induced behavior
146 CL-deficient cells exhibited decreased GFP-tagged mitochondrial proteins inside the vacuole and dec
149 traps can impose a novel localization on GFP-tagged target proteins and results in their controlled m
151 d the temporal and spatial delivery of GFP11-tagged effectors during infection with the foliar pathog
152 fixed seconds to hours after injecting gold-tagged albumin, negatively charged gold nanoparticles, a
153 de-off, we studied the daily routines of GPS-tagged individuals of the world's largest terrestrial so
154 of 2 amino acid mutations and a modified HA tag at the C terminus of PB1, which is sufficient to att
157 magnetic immunocapture to rapidly purify HA-tagged mitochondria from homogenized mammalian cells in
160 he catalytic properties of the hexahistidine tag should be taken into consideration when choosing a s
162 and immobilization of SrtA containing a His-tag from the fermentation supernatant onto a nickel-modi
164 ing human thrombin, PDGF-BB, Avidin, and His-tagged recombinant protein, were studied, and the result
165 specific activity of TopA expressed from His-tagged fusion construct in the chromosome is inversely p
166 s expression and partial purification of His-tagged CesA5 from Physcomitrella patens Immunoblot analy
171 ent immune cells and antibodies to identify, tag, destroy, and eliminate pathogens and infected or da
178 SW480) were engineered to express inducible tagged SPDEF or vector (control) and subcutaneously inje
179 trates by combining a lysine-less internally tagged ubiquitin (INT-Ub.7KR) with SILAC-based mass spec
181 In this new system, a protein-of-interest is tagged with an N-terminal split RNAP (RNAPN), and multip
185 e of L. plantarum strains using the isobaric tags for relative and absolute quantitation (iTRAQ) base
188 ocalization of organelle proteins by isotope tagging (LOPIT), which combines biochemical cell fractio
189 that 4-PBA triggers the secretion of a KDEL-tagged luminal resident, implying that a compromised ret
192 enzymatic biosensor based on an ionic liquid tagged cobalt-salophen metal complex (Co-salophen-IL) im
193 d by coupling mitoxantrone with ionic liquid tags, and cytotoxic behavior of the designed conjugate w
194 ad-through of poly(A) produces a poly-lysine tag, which might alter the localization and solubility o
196 ally available carbonyl-reactive tandem mass tags (aminoxyTMT) enable multiplexed quantification of g
197 multiplexed up to 10-plex using tandem mass tags (TMT) reagents, further increasing throughput, albe
198 le knockout cells using isobaric tandem mass tags reveals targets of degradation, including antagonis
199 h the labeling of specific ligands with mass tags enables detection and quantification of more than 4
200 bling of the molecular brightness of mCherry-tagged beta1-integrins measured using fluorescence corre
202 ntified the binding of individual mNeonGreen-tagged Syk molecules as they associated with the plasma
203 or repulsive interactions between molecular tags that are distributed in gradients in the retina and
206 ys 63 (K63)-linked polyUb chains that do not tag substrates for degradation but rather modify their p
208 roach shows a relationship between choice of tag and the total expression of labelled protein, which
209 stem niche modelling, and showed that 80% of tagged whale positions was near (<7 km) the closest suit
210 input requirement by linear amplification of tagged DSBs by in vitro transcription; (3) quantificatio
211 single-cell analysis with overexpression of tagged signaling proteins to study the dependence of sig
215 smitter via injection has reduced the tag or tagging effect bias associated with studying small fishe
216 tag basis (ie, each DCS stimulation site or "tag" was considered a separate data point across all pat
217 e employed to insert a chemically orthogonal tag into a complex natural scaffold affording the possib
224 ng of SipA with the small fluorescent phiLOV tag, which can pass through the type three secretion sys
226 ining an extracellular pH-sensitive pHluorin tag allow direct visualization of integrin exocytosis in
227 ese hydrolases acquire a mannose 6-phosphate tag by the action of the GlcNAc-1-phosphotransferase enz
228 n (for lamin A) or a MAPLE3 photoconvertible tag (for lamin B1) and membrane phospholipid incorporati
229 nd molecular modeling to strategically place tags and fluorescent proteins within GSDMD that support
230 IL13 "tag" single nucleotide polymorphisms (tag SNPs) in 367 challenge-proven food allergic cases, 1
233 (PR)50- and (GR)50-green fluorescent protein tagged dipeptides were present in the nucleus and nuclea
235 e demonstrated dual-color endogenous protein tagging with sfCherry211 and GFP11, revealing that endop
236 rthogonal tethering to SNAP and CLIP protein tags to create a family of light-gated metabotropic glut
237 ar donors by introducing fluorescent protein tags in human cells and mouse embryos using PCR fragment
238 s with monoallelic green fluorescent protein tags labeling 10 proteins representing major cellular st
239 analysis of machines assembled with protein-tagged components or from different deletion mutants, we
241 Patched (Ptc) and Smoothened (Smo) proteins tagged with GFP or mCherry and expressed at physiologica
245 ervation, we biochemically isolated reporter-tagged Mbp mRNA granules from primary cultured mammalian
249 (b) Autumn migration routes of 12 satellite tagged adult European honey buzzards (colour-coded lines
250 pes, as these new phenotypes serve as secret tags that are difficult for defector to discover and cha
251 ent a full-passive flexible multigas sensing tag for the determination of oxygen, carbon dioxide, amm
254 ethods, antibodies are labeled with sequence tags that can be read out with microfluidic barcoding an
255 with various affinity tags, including single tags 3xFLAG, 3xHA, 3xStrep-tag II, 18xHis, 13xMyc, and d
256 of live cells was demonstrated using a SNAP-tag approach to install the boronic acid reagent onto th
259 were able to develop a CL-activated "sticky tag" that converts the low CL signal into a stable and l
263 rminally tagged with 3xFLAG and C-terminally tagged with 18xHis, we isolated SigT-interactive protein
265 eventive action of this drug indicating that tag diversity may be used as a predictive biomarker.
268 ew transmitter via injection has reduced the tag or tagging effect bias associated with studying smal
269 ly captured by protein G Sepharose, with the tag being removed subsequently using PreScission proteas
271 chemistries, synchronous biosynthesis of the tagged metabolite and its subsequent chemical modificati
272 logical signaling and desensitization of the tagged receptors were not different from wild type recep
278 newly engineered transposable element Dsg to tag a gene that gives rise to a defective kernel (dek) p
279 Here we apply CRISPR-Cas9 gene editing to tag a cytoskeletal protein (alpha-tubulin) and demonstra
280 s develop beta1 integrins carrying traceable tags in the extracellular domain; a pH-sensitive pHlouri
282 ciple applications of the duplex and triplex tags for quantitative proteomics using high-resolution M
283 osyltransferase (UPRT), a method known as TU-tagging, has been used in multiple systems but can have
284 of this system, the proteasome, by ubiquitin tags, but ubiquitin is also used as a signal in other ce
285 d by the size and structure of the ubiquitin tags, which are recognized by receptors associated with
286 elop protein aggregates containing ubiquitin-tagged proteins within cardiac myocytes related to prote
291 % of the identified T4-like viruses in viral tagging experiment infect Synechococcus, while only abou
294 istance of the PubTator text-mining tool, we tagged more than 10 000 articles to assess the ratio of
295 DNA methylation of HDAC4 CpG sites were tagged by a nearby single-nucleotide polymorphism (rs757
298 nes as demonstrated by the fact that the YFP-tagged SYP72 localized to the ER in wild-type plants but
299 on, HEK293 cells stably transfected with YFP-tagged R753Q TLR2 displayed reduced recruitment of MyD88
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