ライフサイエンスコーパス: tandem affinity purification

1 t homogeneity in a highly active state using tandem affinity purification.

2 in the ER by a proteomics approach based on tandem affinity purification.

3 polyacrylamide gel electrophoresis gel after tandem affinity purification.

4 other RNA-binding proteins was confirmed by tandem affinity purification.

5 Here, by using a combination of tandem affinity purification and coimmunoprecipitation,

6 all the subunits of the Arabidopsis AP-2 by tandem affinity purification and found that one of the l

7 Accordingly, we used tandem affinity purification and LC-MS/MS to search for

8 E7 associated cellular protein complexes by tandem affinity purification and mass spectrometry and i

9 A-HYPERSENSITIVE GERMINATION3 as revealed by tandem affinity purification and mass spectrometry prote

10 regulatory sites, we used NF-kappaB p65 in a tandem affinity purification and mass spectrometry prote

11 In this study, we used a transgenic tandem affinity purification and mass spectrometry strat

12 nd how MITF regulates transcription, we used tandem affinity purification and mass spectrometry to de

13 isms of Tax function and regulation, we used tandem affinity purification and mass spectrometry to id

14 Finally, using tandem affinity purification and mass spectrometry, we d

15 Using tandem affinity purification and mass spectrometry, we f

16 Using tandem affinity purification and mass spectrometry, we i

17 ns, as novel Cby1-interacting partners using tandem affinity purification and mass spectrometry.

18 ts associated proteins were identified using tandem affinity purification and mass spectrometry.

19 We have used tandem affinity purification and mass spectroscopy to is

20 in-linked Escherichia coli proteome by using tandem affinity purification and nanospray microcapillar

21 Tandem-affinity purification and quantitative mass spect

22 argeting, we employed in vivo cross-linking, tandem affinity purification, and mass spectrometry to i

23 Here, we have applied a tandem affinity purification approach coupled with direc

24 In this study, we used the tandem affinity purification approach in combination wit

25 In this study, by utilizing the tandem affinity purification approach we have identified

26 eins in HeLa cells were identified using the tandem affinity purification approach.

27 1, which are co-purified during Ymr291w/Tda1 tandem affinity purification, as well as protein kinase

28 Using a tandem affinity purification-based mass spectrometry scr

29 s the analysis of DNA targets under the same tandem affinity purification (BioTAP) regimen.

30 subunit in the affinity-purified PCFS4-TAP (tandem affinity purification) complex involved in the al

31 We have now identified, by tandem affinity purification coupled with mass spectrome

32 carboxyl-terminal globular domain of Mlp1 by tandem affinity purification coupled with mass spectrome

33 Tandem affinity purification demonstrated that RRP1B phy

34 translationally fuse FLAG, HA, cMyc, AcV5 or tandem affinity purification epitope tags onto target pr

35 ng of the growing maize (Zea mays) leaf with tandem affinity purification followed by mass spectromet

36 Tandem affinity purification followed by mass spectromet

37 With preparative tandem affinity purification followed by MS analysis, we

38 mbly of PARC- and CUL7-containing complexes, tandem affinity purification followed by multidimensiona

39 Here, we analyzed RNPs isolated by tandem-affinity purification from TGS1 and tgs1Delta yea

40 ly tagged GlnK and GlnB proteins followed by tandem affinity purification in combination with top-dow

41 ication of CUGBP2-interacting proteins using tandem affinity purification leads to the demonstration

42 Tandem affinity purification linked one candidate, LSM12

43 In this study, a tandem affinity purification-mass spectrometry strategy

44 We used tandem affinity purification-mass spectrometry, co-immun

45 , we analyzed the DDX6 interactome using the tandem-affinity purification methodology coupled to mass

46 o structural features of plant CPSF, we used tandem affinity purification methods to isolate the inte

47 Tandem-affinity purification MS/MS and co-immunoprecipit

48 Here, we use mass spectrometry and tandem affinity purification of a Rik1-TAP fusion protei

49 Tandem affinity purification of CAF-1-interacting protei

50 C-terminus-mediated activities, we undertook tandem affinity purification of E1A-associated proteins.

51 Tandem affinity purification of fission yeast PRMT3 coup

52 uitylating enzyme (USP15) were identified by tandem affinity purification of HPV16 E6-associated prot

53 Tandem affinity purification of NTAP-SOS2-containing pro

54 Tandem affinity purification of protein complexes using

55 Based on tandem affinity purification of Raa7 and mass spectromet

56 to the organelle RNA editosome, we performed tandem affinity purification of the plastidial CHLOROPLA

57 To investigate hSSB1 function, we performed tandem affinity purifications of hSSB1 mutants mimicking

58 Through tandem-affinity purification of human DDB1 and CUL4A com

59 ania tarentolae mitochondria isolated by the tandem affinity purification procedure (TAP).

60 A tandem affinity purification procedure revealed that COU

61 .85 A resolution using protein purified by a tandem affinity purification procedure.

62 This modified form of tandem affinity purification produces highly purified pr

63 SNAP near-infrared imaging and tandem-affinity purification revealed the ADRA1D is expr

64 Tandem affinity purification reveals that Iduna binds to

65 Through a tandem affinity purification strategy and mass spectrome

66 In this study, we used a tandem affinity purification strategy to identify protei

67 Using a tandem-affinity purification strategy and mass spectrome

68 ity-purified Drs2p, possessing an N-terminal tandem affinity purification tag (TAPN-Drs2), retains AT

69 By using the tandem affinity purification tag approach, we identified

70 of recombinant vaccinia viruses that have a tandem affinity purification tag attached to A56, K2, or

71 only one of the three beta subunits using a tandem affinity purification tag on the C terminus of th

72 We devised a tandem affinity purification tag termed the HPB tag, whi

73 pressed an N-terminal fusion of the improved tandem affinity purification tag to SOS2 (NTAP-SOS2) in

74 AP-1-like (YAP1) transcription factor and a tandem affinity purification tag, we detected approximat

75 Tandem affinity purification-tagged Lsm3p was associated

76 Using tandem affinity purification-tagged Pan1p, we identified

77 REL1 were expressed as enzymatically active tandem affinity purification-tagged proteins in a Baculo

78 xon cleavage intermediate are recovered by a tandem affinity purification-tagged Spp382 derivative.

79 We isolated Tic56 by copurification with Tandem Affinity Purification-tagged Toc159 in the absenc

80 Tandem affinity purification-tagged Trypanosoma brucei R

81 Tandem-affinity purification-tagged dynamitin AA 1-87 bi

82 compiling the in vivo interaction data from tandem affinity purification tagging as well as other av

83 We validate our predictions with TAP (tandem affinity purification) tagging experiments.

84 In this work we have used tandem affinity purification tags to isolate the Paf1 co

85 tably expressing PS paralogs with N-terminal tandem-affinity purification tags served as biological s

86 ibes a method that we developed to adapt the tandem affinity purification (TAP) approach for use in m

87 We translated an optimized tandem affinity purification (TAP) approach from Arabido

88 report here that using an unbiased approach, tandem affinity purification (TAP) followed with mass sp

89 3p that might act to regulate this factor, a tandem affinity purification (TAP) moiety was fused to P

90 Here we describe the tandem affinity purification (TAP) of Cdc5p- and Cef1p-a

91 easing background are presented: whereas (1) tandem affinity purification (TAP) of tagged proteins of

92 ial extracts of Leishmania tarentolae by the tandem affinity purification (TAP) procedure, using thre

93 Tandem affinity purification (TAP) proteins including fr

94 Tandem affinity purification (TAP) strategies constitute

95 s encoding protein kinases were fused to the tandem affinity purification (TAP) tag and expressed in

96 recombinant HCMV, NTAP97, which expresses a tandem affinity purification (TAP) tag at the amino term

97 A synthetic gene encoding the tandem affinity purification (TAP) tag has been construc

98 vel multiple affinity purification (MAFT) or tandem affinity purification (TAP) tag has been construc

99 were generated that contained either a small tandem affinity purification (TAP) tag or the green fluo

100 Single epitope tags and tandem affinity purification (TAP) tags have been used t

101 When isolated by tandem affinity purification (TAP), the other protein co

102 based on in-cell chemical cross-linking and tandem affinity purification (TAP), we herein identify s

103 ften used for purification of protein A- and tandem affinity purification (TAP)-tagged proteins from

104 s from the S. coelicolor lysate based on the tandem affinity purification (TAP).

105 mplexes were isolated from infected cells by tandem affinity purification (TAP).

106 We also describe a tandem affinity purification (TAP)/floxed marker gene pl

107 We previously developed a novel tandem-affinity purification (TAP) approach using hexahi

108 study, terminase complexes were isolated by tandem-affinity purification (TAP) using recombinant vir

109 se and stem cell model by knock-in (KI) of a tandem-affinity-purification (TAP) epitope at the endoge

110 Using tandem affinity purification techniques coupled with MS,

111 Using tandem affinity purification techniques, we identified D

112 Using tandem affinity purification technology, we provide evid

113 )-PAGE analysis, co-immunoprecipitation, and tandem affinity purification that the two mt OM proteins

114 eversibly inhibit Ubl proteases) before TAP (tandem affinity purification) that allows for efficient

115 Here, we use tandem affinity purification to determine the interactin

116 onothiol GRX encoded by GRXS17 Here, we used tandem affinity purification to establish that Arabidops

117 question, we used the proteomic approach of tandem affinity purification to identify cellular protei

118 que combining cell-surface biotinylation and tandem-affinity purification to study a region of the pr

119 by expressing each CEP4 double mutant from a tandem affinity purification vector followed by affinity

120 Reciprocal tandem affinity purifications verify that Msl5 exists in

121 Using the approach of tandem affinity purification we recently discovered that

122 Using tandem affinity purification, we found JAZ12 to interact

123 Using tandem affinity purification, we identified heat shock p

124 Using tandem affinity purification, we identified human NF45 a