ライフサイエンスコーパス: target DNA

1 he dUTPase activity and Stl release from its target DNA.

2 uations that lead to a slow clearance of the target DNA.

3 aspects of interactions between antigens and target DNA.

4 lts in a staggered seven-nucleotide break of target DNA.

5 partially matches a segment (protospacer) in target DNA.

6 the two viral DNA molecules and help capture target DNA.

7 ifically targets and cleaves both strands of target DNA.

8 nsor due to hybridization of the ss-DNA with target DNA.

9 s predominantly a tetramer when bound to its target DNA.

10 and preserved in vitro binding affinity for target DNA.

11 are important for accurate amplification of target DNA.

12 compare the response to single stranded (ss) target DNA.

13 CTD beta1-beta2 loop were predicted to bind target DNA.

14 initial amount, or copy number (N0), of the target DNA.

15 de signal amplification for the detection of target DNA.

16 nces and a 2.8 kb excised mini-casposon into target DNA.

17 onors that were LRET-paired with Cy3-labeled target DNA.

18 problem by obviating direct labeling of the target DNA.

19 n RGO surface and its hybridization with the target DNA.

20 t was able to recognize the other end of the target DNA.

21 Nrf1 and Nrf2 and two directly interact with target DNA.

22 poson) is the only complex able to capture a target DNA.

23 tion to form the functional complex with its target DNA.

24 ructures through specific hybridization with target DNA.

25 RVR and RR variants bound to a crRNA and its target DNA.

26 oining of newly excised transposon ends with target DNA.

27 cing site-specific double-stranded breaks in target DNA.

28 dimerization while preventing binding to the target DNA.

29 be, which was complementary with the part of target DNA.

30 orthologs, in complex with an sgRNA and its target DNA.

31 LE and its affinity, for both target and non-target DNA.

32 luster labeled reporter DNA hybridize to the target DNA.

33 in the free protein and in the complex with target DNA.

34 plex formation induce rapid rejection of off-target DNA.

35 by the immobilized probe DNA and hybridized target DNA.

36 Rous sarcoma virus in complex with viral and target DNAs.

37 he differential discrimination of mismatched target DNAs.

38 e probe DNA is proportional to the number of target DNAs.

39 One system (type I-F) targets DNA.

40 s9 methods aiming to reduce transcription by targeting DNA.

41 s) before acquiring and dwelling at specific target DNA (12.0-14.6 s).

42 here was a good linear relationship (F=0.57 [target DNA]+21.31, R(2)=0.9984) between the fluorescent

43 or PAM duplex recognition, as well as blocks target DNA access to key catalytic residues lining the R

44 We also find that the conformation of the target DNA after strand transfer is involved in preventi

45 Affinity for non-target DNA also increases non-linearly with the number o

46 (831 molecules in 35.4 nl assay volume) for target DNA and 16 fM (338 molecules) for target RNA afte

47 n reactions among the FCN-labeled DNA probe, target DNA and capture DNA probe were performed on the l

48 e PCG2 employs an unusual mode of binding to target DNA and demonstrate the versatility of wHTH domai

49 It reveals severe distortion of target DNA and flipping of the target adenines into extr

50 PR-Cas system results in the cleavage of the target DNA and its transcripts, mediated by independent

51 orm co-transcriptional cleavage of the viral target DNA and its transcripts.

52 of alkyldiamine side chains was designed to target DNA and RNA G-quadruplexes (G4) in the promoter a

53 il at the 3' end), for efficient ligation to target DNA and subsequent PCR amplification primed by th

54 we investigate the interaction of Cas9 with target DNA and use our findings to improve HDR efficienc

55 stal structure of I-CvuI in complex with its target DNA and with the target DNA of I-CreI, a homologu

56 within a range of 1 x 10(8)-10(11) copies of target DNA, and a linear relationship between the log co

57 levels, the binding of nuclear STAT3 to its target DNA, and the expression of downstream targets of

58 e detection of as little as 9.6 attograms of target DNA, and we show that its performance is comparab

59 f the Dlx5 homeodomain to recognize and bind target DNAs, and they likely destabilize the formation o

60 cer DNA with free 3'-OH ends and supercoiled target DNA are required, and integration occurs preferen

61 rforms subsequent site-specific detection of target DNA as a field-effect transistor.

62 1) bound to sgRNA as a binary complex and to target DNAs as ternary complexes, thereby capturing cata

63 tion of the Egr-1 zinc-finger protein in the target DNA association process.

64 pyogenes Cas9 in complex with sgRNA and its target DNA at 2.5 A resolution.

65 sCpf1) in complex with the guide RNA and its target DNA at 2.8 A resolution.

66 amplification method, we were able to detect target DNA at concentrations as low as 0.5 nM with the n

67 is ultrasensitive, allowing the detection of target DNA at femtomolar level by simple spectroscopic a

68 protospacer adjacent motif (PAM) and cleaves target DNA at high efficiency with a variety of guide RN

69 The method is capable of detecting target DNA at the concentration down to 0.85 pM in 60min

70 s the direct, irreversible conversion of one target DNA base into another in a programmable manner, w

71 ated spacer integration requires IHF-induced target DNA bending and explain the elusive role of CRISP

72 e use Mu to investigate the ramifications of target DNA bending on the transposition reaction.

73 Rct) was linear with the number of copies of target DNA between 150 and 10(6) copies/ml.

74 found no significant difference in in vitro target DNA binding affinity of recombinant wild-type p53

75 Non-target DNA binding affinity scales with PAM density, and

76 tors, much needs to be learned regarding the target DNA binding by yet-to-be characterized RNPP regul

77 or the biophysical constraints governing off-target DNA binding.

78 e predict optimal nucleotide composition for targeting DNA-binding antibodies.

79 ed atomic force microscopy (AFM) to detect a target DNA bound to small (1.4-1.9 mum diameter) probe D

80 air pathways following the introduction of a targeted DNA break is essential to further advance the s

81 can drive lymphomagenesis by generating off-target DNA breaks at loci that harbor highly active enha

82 in developing lymphocytes by generating "on-target" DNA breaks at matched pairs of bona fide recombi

83 h p53 and interferes with its binding to the target DNA but also functions as a negative regulator of

84 ly provides the efficient separation for the targeted DNA but can also maintain the bioactivity of as

85 r highly sensitive and specific detection of target DNA by employing the nonlinear hybridization chai

86 ample, we use PITC to detect the presence of target DNA by monitoring the hybridization-induced bindi

87 in whole blood prevent the amplification of target DNA by RPA.

88 ence of target DNA, the Exo III recycles the target DNA by selectively digesting the dye-tagged seque

89 rement with a detection limit of 6x10(-12) M target DNA (calibration curve r(2)=0.98).

90 As a result, the target DNA can be specifically detected in serum-contain

91 The approach combines targeted DNA capture with a high-throughput reporter gen

92 ntary base pairing between the guide RNA and target DNA, Cas9-DNA interactions, and associated confor

93 nome engineering, namely the inefficiency of targeted DNA cassette insertion.

94 The presence of single or multiple target DNAs causes the corresponding single-stranded DNA

95 ng sgRNA design to improve the efficiency of target/DNA cleavage is critical to ensure the success of

96 ave yielded improvements in the precision of targeted DNA cleavage, but they often restrict the range

97 tructures in minimizing the formation of off-target DNA complexes.

98 In the first AND gate, a chimeric target DNA comprising of four biomarkers was hybridized

99 Our investigation revealed that a target DNA concentration as low as 5.0x10(-21)M can tran

100 4) between the fluorescent intensity and the target DNA concentration in the samples.

101 lateral flow-based method for enriching the target DNA concentration relative to the background DNA

102 ls a linear correlation (R(2) = 0.98) to the target DNA concentration, with a limit of detection down

103 ction-purification process revealed that low target DNA concentrations (80 pg/muL) can be successfull

104 of LbCpf1 in complex with the crRNA and its target DNA containing either TTTA, TCTA, TCCA, or CCCA a

105 They improve strand transfer when the target DNA contains a mismatch near the TA dinucleotide.

106 he working solution was only 100 muL and the target DNA could be detected at a concentration down to

107 n between ssDNA probe on this DNA sensor and target DNA creates nanomechanical bending and resistance

108 t study investigated the role of ERH and its target DNA damage repair genes in hepatocellular carcino

109 allow for proper control of toxicity from on-target DNA damage.

110 mage response proteins ATR in HCC cells, and targeting DNA damage response by Chk1 inhibitor augments

111 mediated by ZMYND8 and the NuRD complex that targets DNA damage, including when it occurs within tran

112 Analysis of a HIF-3 target, DNA-damage-inducible transcript 4, a key surviva

113 his method showed a good specificity for the target DNA detection, with the capabilty to discriminate

114 onto compact discs is proposed for real-time targeted DNA determination.

115 dues W159, R186, F187 and K190 stabilise the target DNA distortions and are required for efficient tr

116 nal change upon hairpin hybridization to the target DNA, dominated by the "on-off" signal change mode

117 argeting and gene disruption downstream of a targeted DNA double-strand break.

118 Targeted DNA double-strand breaks have been shown to sig

119 tion at predefined genomic sites by creating targeted DNA double-strand breaks, there are only a hand

120 Generation of targeted DNA DSBs within Fos and Npas4 promoters is suff

121 considering the invasion of guide RNAs into targeted DNA duplex.

122 ith Probe-based DNA Enrichment by RNA probes targeting DNA duplex (DEEPER-Seq).

123 RecBCD targets DNA ends at collapsed forks, enabling DNA captur

124 rget commitment by limiting the stability of target DNA engagement until an appropriate insertion sit

125 In the presence of target DNA, FCNs were captured on the test zone of the b

126 d with kinetically favored Sox2 engaging the target DNA first, followed by assisted binding of Oct4.

127 In the presence of target DNA, formation of the anionic DNA-acpcPNA duplex

128 The target DNA fragment of 18s rRNA gene was amplified and h

129 PCR at a lower concentration to amplify its target DNA fragment.

130 Target DNA fragments at 10 fM concentration (approximate

131 ncreases linearly with time and purify 25 nt target DNA from 10,000-fold higher abundance background

132 agnetic bead-based method for the capture of target DNA from a pool of interfering genomic DNA.

133 exponentially amplifying very low amounts of target DNA from genetic, clinical, and forensic samples.

134 from 1 pM to 1000 pM, and could discriminate target DNA from mismatched sequences.

135 lones are broad-spectrum antibacterials that target DNA gyrase by stabilizing DNA-cleavage complexes,

136 nd aminocoumarin antibiotics, compounds that target DNA gyrase in bacteria.

137 ied a class of antibacterial thiophenes that target DNA gyrase with a unique mechanism of action and

138 Fluoroquinolone antibacterials, which target DNA gyrase, are critical agents used to halt the

139 ocally that A. thaliana encodes an organelle-targeted DNA gyrase that is the target of the quinolone

140 c produced by Streptomyces antibioticus that targets DNA gyrase.

141 facilitating zippering up of 20-bp guide RNA:target DNA heteroduplex on ternary complex formation.

142 In this method, target DNA hybridized with probe DNA modified on AuNP, a

143 s been successfully applied for detection of target DNA in complex sample matrices.

144 ticles and fluorescent DNA probes to capture target DNA in free solution, and we demonstrate that the

145 uctures of Cas1-Cas2 bound to both donor and target DNA in intermediate and product integration compl

146 el-free, and amplification-free detection of target DNA in low concentrations, low percentages, and v

147 were demonstrated to capture and enrich the target DNA in the hybridization buffer or human plasma.

148 , enabling a reproducible detection of 25mer target DNA in the low nanomolar range.

149 linearly with logarithm of concentration of target DNA in the range 1x10(-13)-1x10(-6) M.

150 strating multiplex sensing of the PDGF and a target DNA in the same solution.

151 ned detection limit was circa 8x10(-13) M of target DNA in the sample which is a substantial improvem

152 late for homology-directed repair leading to targeted DNA insertion and gene correction.

153 zebrafish, improving current approaches for targeted DNA integration in the genome.

154 yotic or eukaryotic cells and integrates the target DNA into the genome.

155 pacer adjacent motif (PAM) sequence flanking target DNA is crucial for self versus foreign discrimina

156 raction of the FOXM1 DNA binding domain with target DNA is essential for recruitment.

157 are better targets for the capture when the target DNA is nicked two nucleotides apart from the TA.

158 triggers binding but extensive pairing with target DNA is required for cleavage.

159 DBD) in complex with its high-affinity viral target DNA, LANA binding site 1 (LBS1), at 2.9 A resolut

160 To promote HDR at the expense of NHEJ, we targeted DNA ligase IV, a key enzyme in the NHEJ pathway

161 of length on affinity for target versus non-target DNA manifests in specificity increasing then dimi

162 one accession move across a graft union and target DNA methylation de novo at normally unmethylated

163 pendent forms of RdDM function to critically target DNA methylation to full-length and transcriptiona

164 ngineered DNA methyltransferases (MTases) to target DNA methylation to specific sites in the genome w

165 We used this system to target DNA methylation within the p16(Ink4a) promoter in

166 e 24-nt small interfering RNAs (siRNAs) that target DNA methylation.

167 n and minimize the unintended effects of off-target DNA methylation.

168 facile and convenient approach for efficient targeted DNA methylation by fusing inactive Cas9 (dCas9)

169 catalytically inactive Cas9 (dCas9) enables targeted DNA methylation editing.

170 g development is still largely restricted to target DNA methylome, emerging evidence indicates that h

171 associated with reduced activity of the E2F target, DNA methyltransferase 1.

172 Consistent with miR-29's role in targeting DNA methyltransferase 3A (DNMT3A), a key enzym

173 pression of epigenetically silenced genes by targeting DNA methyltransferases.

174 single-guide RNA (sgRNA), can result in off-target DNA modification that may be detrimental in some

175 alized with DNA probes, enabled detection of target DNA molecules (10-200 nM) in physiologically rele

176 gle-DNA molecule/TIRS nanotag hybridization, target DNA molecules of H7N9 were detected down to 74 zM

177 tool in this regard; its ability to recycle target DNA molecules results in markedly improved detect

178 ishing perfect-matched and single-mismatched target DNA molecules to the best extent, likely due to t

179 Domains (MBD)-based enrichment method, which targets DNA molecules containing methylated CpGs.

180 L initiative assessed cases by whole-genome, targeted DNA, mRNA and microRNA sequencing and CpG methy

181 em is emerging as a robust biotechnology for targeted-DNA mutation.

182 EA, using light-induced release of DHEA from targeted DNA nanocapsules.

183 in complex with its target DNA and with the target DNA of I-CreI, a homologue enzyme widely used in

184 By leveraging this technique, target DNA of Sus scrofa (pork) meat was detected as low

185 (MQDS) was developed to identify and measure target DNAs of pathogenic microorganisms and eliminated

186 Enhancing the flexibility of the target DNA or prebending it increases its affinity for t

187 ged on whether some CRISPR systems naturally target DNA or RNA.

188 e biosensor had good selectivity towards the target DNA over the non-specific sequences and also it w

189 The effect of a target DNA overhang on the hybridization efficiency was

190 A longer target DNA overhang was found to provide a better respon

191 The target DNA partly hybridizes with capture probe on the g

192 ex with transposon ends covalently joined to target DNA, portrays the transposition machinery after D

193 able flap can then be actuated by a specific target DNA present in a sample, by exposing a hemin/G-qu

194 Virus intasomes suggests a binding mode for target DNA prior to Mos1 transposon integration.

195 hly tested for the first time, regarding the targeted DNA quality and quantity.

196 to be 100 fg by testing serial dilutions of target DNA ranging from 1 ng to 1 fg per reaction, and n

197 utline our state-of-the-art understanding of target DNA recognition and cleavage by CRISPR-Cas9 nucle

198 handle disrupted its binding to Cascade and target DNA recognition.

199 tward-directed bases, has a critical role in target DNA recognition.

200 s have gained broad appeal as a platform for targeted DNA recognition, largely owing to their simple

201 ntify more than 5800 instances in which TRFs target DNA regions with demonstrated enhancer activity.

202 ll apoptosis, we reasoned that rLOX-PP could target DNA repair pathways typically elevated in cancer.

203 Therapeutic strategies targeting DNA repair pathway defects have been widely ex

204 considerably increases the opportunities for targeting DNA repair-defective tumors.

205 DNA repair templates results in precise and targeted DNA replacement with as much as approximately 1

206 Thus targeting DNA replication and G2-M cell cycle checkpoint

207 ked in at a 1000-fold and 800-fold excess of target DNA, respectively, demonstrating the assay's abil

208 ementary and noncomplementary strands of the target DNA, respectively.

209 ybridization between the hairpin DNA and the target DNA, resulting in the significant decrease of the

210 one or bound to single-guide RNA (sgRNA) and target DNA revealed a bilobed protein architecture that

211 LOF methods target DNA, RNA or protein to reduce or to ablate gene f

212 mediately "activated" and enabled to capture target DNA/RNA efficiently from the opposite side of the

213 rase together with the chimeric nucleotides, target DNAs/RNAs trigger the release of stoichiometrical

214 fer (also known as "direct transfer") in the target DNA search process.

215 sis of metal complexes that can specifically target DNA secondary structure has attracted considerabl

216 Targeting DNA secondary structures thus represents a pot

217 ansfer of the recessed 3'-hydroxyls into the target DNA separated by 4-6 bp.

218 udied tumor suppressor gene, was used as the target DNA sequence model.

219 ould exclusively and specifically detect the target DNA sequence of B. cereus from other bacteria tha

220 sensors were prepared for the detection of a target DNA sequence on the p53 tumor suppressor (TP53) g

221 iption factor Egr-1, which recognizes a 9-bp target DNA sequence via three zinc-finger domains, rapid

222 In the presence of the complementary target DNA sequence, the probes will compete for binding

223 g 20 nucleotides that are complementary to a target DNA sequence.

224 r suppressor gene, was used as the detection target DNA sequence.

225 e resolution after bisulfite conversion of a target DNA sequence.

226 facilitate rapid bacterial identification by targeted DNA sequence analysis or by whole-genome sequen

227 A (sgRNA) directs the endonuclease Cas9 to a targeted DNA sequence for site-specific manipulation.

228 becomes arrested immediately upstream of the targeted DNA sequence, and is not rescued by transcripti

229 Large-scale mutagenesis of target DNA sequences allows researchers to comprehensive

230 face to facilitate the selection of the best target DNA sequences for experimental design.

231 residue (Gln56) to accommodate variation in target DNA sequences from diverse rhizobial genes for no

232 To test whether target DNA sequences modulate protective mechanisms via

233 ns bearing missense mutations failed to bind target DNA sequences on EMSA and confocal microscopy; ho

234 kable (down to aM) detection sensitivity for target DNA sequences present in solution.

235 otein binding and nuclease activation at off-target DNA sequences remains elusive.

236 lities to cleave any of 10(12) potential off-target DNA sequences using in vitro selection and high-t

237 d zinc finger domains involved in binding to target DNA sequences.

238 rough preventing the binding of HMGA2 to the target DNA sequences.

239 anipulation of cis-acting and/or chromosomal target DNA sequences.

240 in vivo and enables continuous evolution of targeted DNA sequences.

241 function and was infrequently identified by targeted DNA sequencing in invasive strains of the same

242 Exome sequence analysis with validation by targeted DNA sequencing of 125 samples uncovered, in add

243 Here, Patel et al. use targeted DNA sequencing of MODY patients and large-scale

244 e, we performed whole-genome, exome, RNA and targeted DNA sequencing on 65 infants (47 MLL-R and 18 n

245 Targeted DNA sequencing was performed on 243 (34.9%) pat

246 insertion sites are typically identified by targeted DNA-sequencing and subsequently assigned to pre

247 Target DNA serves as a template for two DNA probes, one

248 he specially designed capture probes and the target DNA significantly changes the thermophoretic prop

249 tide (brHis2) does not bind to its consensus target DNA site (5'-GTCAT-3').

250 Rgamma:RXRalpha heterodimer to more than 300 target DNA sites and variants thereof.

251 In human cells, fCas9 modified target DNA sites with >140-fold higher specificity than

252 gene expression through binding to specific target DNA sites.

253 by transcription factors binding to specific target DNA sites.

254 The target DNA spiked food matrix (chicken meat) is also suc

255 tively occupies both PAM-interacting and non-target DNA strand cleavage catalytic pockets.

256 tion the HNH nuclease domain adjacent to the target DNA strand cleavage site in a conformation essent

257 lly favorable coordination ready for the non-target DNA strand cleavage.

258 Hybridization to a target DNA strand tethers the beads together, inducing b

259 with wedge insertion, initiating directional target DNA strand unwinding to allow segmented base-pair

260 DNA for complementary base-pairing with the target DNA strand while displacing the non-target strand

261 me process also generates a bulge in the non-target DNA strand, enabling its handover to Cas3 for cle

262 te of Cas9 HNH domain primed for cutting the target DNA strand.

263 leotide sequences in both the target and non-target DNA strands and recognizes the 5'-NNNVRYM-3' as t

264 rmations of AacC2c1 with both target and non-target DNA strands independently positioned within a sin

265 to screen a number of labeled and unlabeled target DNA strands to measure the impact of fluorescent

266 their respective viral DNA or branched viral/target DNA substrates have indicated these intasomes are

267 rokaryotic adaptive immune systems generally target DNA substrates, type III and VI CRISPR systems di

268 s on the two viral cDNA ends, docks onto the target DNA (tDNA), and catalyzes viral genome insertion

269 DNA (vDNA) copy of its RNA genome into host target DNA (tDNA).

270 Retroviruses favor target-DNA (tDNA) distortion and particular bases at sit

271 immunity, which blocks Tn7 insertion into a target DNA that already contains Tn7.

272 ay couples rapid isothermal amplification of target DNA that is specific to Mtb with gold nanoparticl

273 hout hybridization between hairpin probe and target DNA, the Ag NCs presented bright fluorescence for

274 In the presence of target DNA, the Exo III recycles the target DNA by selec

275 After binding the nanoparticles with the target DNA, the following sandwich structure was formed:

276 After binding with target DNA, the hairpin shape was destroyed which result

277 demonstrated that the genomic context of the targeted DNA, the GC percentage, and the secondary struc

278 A and the 14th-17th nucleotide region of the targeted DNA, the stabilities of which we find correlate

279 eration was based on: (i) hybridization of a target DNA to a Ramp-oligonucleotide probe conjugate, fo

280 ntional fluorescence assay that requires the target DNA to be fluorescently labeled, the sequential s

281 Small molecules that target DNA to interfere with protein-DNA interactions ma

282 atic cleavage of the RNA probe, allowing the target DNA to liberate and hybridize with another RNA pr

283 Hybridization with the target DNA was studied by measuring the electrochemical

284 tic domains when associated with on- and off-target DNA, we find that DNA cleavage efficiencies scale

285 rstly, we investigate the sensor response to target DNA when the target is in a double stranded (ds)

286 to naturally form an unstable complex with a target DNA, whereas mutant combinations required for lar

287 radation of short single-stranded regions of target DNA, whereas PAM mutations elicit an alternative

288 nce suggests that the type III-A Csm complex targets DNA, whereas biochemical data show that the type

289 black depending on the concentration of the target DNA, which could be recognized by naked eyes.

290 lyses the integration of viral DNA into host target DNA, which is an essential step in the life cycle

291 get RNAs, and Csa systems have been shown to target DNA, while the mechanism by which Cst complexes s

292 When a target DNA with a complementary sequence to that of the

293 The method enabled the analysis of the target DNA with a detection limit corresponding to 1 aM.

294 errogation steps that precede the pairing of target DNA with guide RNA.

295 sgRNA binary complex is stable and binds its target DNA with high affinity, allowing sequential or si

296 NanoGene assay employs the hybridization of target DNA with quantum dot labeled magnetic beads and p

297 he probe, and the signal was compared to the target DNAs with different lengths and overhangs.

298 repeat provides extra binding energy for the target DNA, with the gain decaying exponentially such th

299 mized LFB was capable of detecting of 0.1 nM target DNA without instrumentation.

300 ld sensing chip and the unpaired fragment of target DNA works as a trigger to initiate the nonlinear