ライフサイエンスコーパス: target site

1 were detected more than 150 bp away from the target site.

2 iated by disruption of miR-29 binding to its target site.

3 s of allelic variance at and surrounding the target site.

4 y confirms that ADAM9 3'UTR contains miR-126 target site.

5 ARF18 transcript was cleaved at the OsmiR160 target site.

6 radients mediate cellular trafficking to the target site.

7 a site reconstituting the original A. boonei target site.

8 hin the body and finally deliver them at the target site.

9 ng repair pathway (c-NHEJ), regenerating the target site.

10 he physiological conditions at the molecular target site.

11 rimers predicted to bind specifically to the target site.

12 protospacer adjacent motif (PAM) next to the target site.

13 e migration and invasion to establish at the target site.

14 ation among miRNAs when attempts to discover target sites.

15 comparative genomics, and added CRISPR/Cas9 target sites.

16 uclear residence increases DNA damage at off-target sites.

17 ount in a statistical model to predict their target sites.

18 out escaping the micro-volume containing the target sites.

19 onality precisely matched to homo-oligomeric target sites.

20 ns form RNA-DNA-DNA triplexes with predicted target sites.

21 tions to the experimentally identified miRNA target sites.

22 a, we identified seven new features of miRNA target sites.

23 elps explain how these molecules locate rare target sites.

24 vide a general strategy for identifying Cas9 target sites.

25 of cTnI mediated by PKA at the Ser-23/Ser-24 target sites.

26 nderstanding of the characteristics of miRNA target sites.

27 ent, and direct protein to its intracellular target sites.

28 es; degradome sequencing confirmed 60 of the target sites.

29 o-expression of an Eos mutant lacking miR-17 target sites.

30 ineering and are inserted specifically at TA target sites.

31 ites by promoting transposition to different target sites.

32 nd effector of the epigenetic state at these target sites.

33 f sgRNAs and develop a metric to predict off-target sites.

34 ence specificity in the recognition of their target sites.

35 alth-enhancing properties of anthocyanins at target sites.

36 ency to the Igh locus and to several AID off-target sites.

37 te structural properties of their respective target sites.

38 cessibility determined by sequences flanking target sites.

39 sphoSTAT5B and marked increase of binding to target sites.

40 methods have been developed to predict miRNA target sites.

41 proteins can steer selection of advantageous target sites.

42 d1, depends on threonine identity of its Cdk target sites.

43 uide RNA sequences and predict potential off-target sites.

44 face of genetic drift within potential host target sites.

45 designed binding sites are aligned with the target sites.

46 f repair fragments with homology to the Cas9 target sites.

47 dency to cleave DNA non-specifically at "off-target" sites.

48 ults in small insertions or deletions at the targeted site.

49 assembly and synergistic gene expression at targeted sites.

50 cation that the transplanted cells reach the targeted sites.

51 nd efficient (up to 70%) DNA methylation at targeted sites.

52 ses of CTCF/BORIS-bound regions: single CTCF target sites (1xCTSes) that are bound by CTCF alone (CTC

53 e a new library of aminopyrimidine analogues targeting site-2 of the pleckstrin homology (PH) domain.

54 und by CTCF alone (CTCF-only) or double CTCF target sites (2xCTSes) simultaneously bound by CTCF and

55 cterizing a highly specific pattern at IS186 target-sites, 5'-GGGG(N6/N7)CCCC-3'.

56 the restriction enzymes that share in their target sites a conserved CCGG tetranucleotide and a clea

57 ining genome-wide RIsearch2 predictions with target site accessibilities and transcript abundance est

58 We hypothesize that CAPs regulate DNA target site accessibility through alteration of the rate

59 dividual nucleotide substitutions within the target site affect the extent of cooperativity in PPARga

60 d at one MC site trigger stimuli at a second target site after a fixed delay, the connections between

61 ules supports the selection of anti-parallel target site alignment prior to the chemical steps.

62 Finding the target site and associating in a specific orientation ar

63 ns when one is choosing a nuclease platform, target site and delivery method.

64 complex stationed at the specific miRNA:mRNA target site and oligouridylated by other TUTases at its

65 ans of concentrating therapeutic agents at a target site and the success of this approach has been de

66 agents could eject Zn(2+) from the predicted target site and thus inhibit viral replication.

67 tunity to study the characteristics of miRNA target sites and improve miRNA target site prediction me

68 ependently required for looping between CTCF target sites and insulation of topologically associating

69 3' sequences is prevalent in the majority of target sites and leads to specific targeting by members

70 odel enables the prediction of non-canonical target sites and more accurately resolves miRNA interact

71 ISPR/Cas modifications in 1467 potential off-target sites and no modifications in tumor suppressor ge

72 /deletions (indels) can result in altered on-target sites and novel potent off-target sites, which ca

73 igh frequency of indels was observed at both target sites and one off-target site, while no cleavage

74 search for two different subsets of specific target sites and provide information on the timescales o

75 ld, thus creating the need to discover novel target sites and target-specific compounds for insectici

76 obilities can prevent monomers from reaching target sites and this results in a partial to complete l

77 s as a selfish genetic element, replaces the targeted site and propagates to replace additional wild-

78 rotospacer adjacent motif (PAM) flanking the target site, and subsequent R-loop formation and strand

79 ovide sustained therapeutic drug levels at a target site, and thereby reducing the frequency of dosin

80 ng sprouts, suggesting sampling of potential target sites, and lowered Flt1 levels reduced transient

81 og (Nanog homeobox) and Hoxa1 on many common target sites, and these are linked to genes in the pluri

82 a store-operated Ca(2+) channels to specific target sites, and this process has been described in con

83 tune the local chemical environment around a target site are rare.

84 MoA of bioactive molecules, especially when target sites are complex and hard to reconstitute in vit

85 oing efforts to understand how specific mRNA target sites are selected and how their modification is

86 1 in shaping OCT4 binding reflects how these target sites are used during cellular reprogramming and

87 owever, it binds and cleaves the palindromic target site as a dimer.

88 s9 specificity by inhibiting cleavage of off-target sites at the nucleosome edge.

89 The functional casposase target site bears clear resemblance to the leader sequen

90 imental observation of a compound bound to a target site before chemical optimization and development

91 ntial correlations between m5C and micro RNA target sites, binding sites of RNA binding proteins, and

92 Using target site blockers in vivo, we identify multiple devel

93 g the transcription factor residence time on target sites.Both transcription binding kinetics and pos

94 teins should not only rapidly diffuse to the target site but also dynamically explore multiple local

95 -E549A failed to restore editing at the main target sites but was able to restore editing at the matR

96 iety of mechanisms, including changes to the target site, but is often associated with substantial fi

97 wide identification of AGO binding and miRNA target sites, but the most widely used miRNA target pred

98 somes affect Cas9 binding and activity at on-target sites, but their impact at off-target sites is un

99 -labeled PCR amplicons covering the nuclease target site by capillary electrophoresis in a sequenator

100 The RNA-programmed Cas9 locates the target site by scanning genomic DNA.

101 l purpose: first, directing the particles to target sites by enhancing their surface mean-free paths

102 -end life cycle in a population of saturated target sites by promoting transposition to different tar

103 unds resulted in diminished accessibility at targeted sites by disrupting transcription of EWSR1-FLI1

104 so viability, showing that a single microRNA target site can be essential.

105 sgene that possesses GFP and a perfect piRNA target site can be rapidly and permanently silenced via

106 recombinant mTERF6 bound to its plastid DNA target site can terminate transcription.

107 ed that changes in phosphorylation of kinase target sites can be used to infer when a kinase activity

108 the N7 neuraminidase were less frequent but targeted sites close to the sialic acid binding site.

109 Rz, a database of validated zebrafish CRISPR target sites collected from published sources, as well a

110 Cas9 generates a double-strand break at DNA target sites complementary to the guide RNA and has been

111 Mutation at the target site completely abrogated the activity of miR-650

112 onuclease PacI) that also recognizes an 8-bp target site consisting solely of A:T base pairs.

113 ing and further support the consideration of target site cooperation as a fundamental characteristic

114 Strikingly, both small RNAs and their target sites demonstrated significant overlap with retro

115 enzyme, and provide rules for selecting Cas9 target sites distinct from and complementary to those ba

116 hibit significant bends in the transposition target site DNA.

117 variants can be explained by a shift of the target sites due to the presence of the glycan.

118 insertion was accompanied by a 15-bp direct target site duplication (TSD).

119 Perfect target site duplication is favorable, but not required,

120 A typical ProtoRAG is flanked by 5-bp target site duplications and a pair of terminal inverted

121 generates two noncompatible DNA breaks at a target site, effectively deleting the majority of the ta

122 frequency energy application rendered 74% of targeted sites electrically unexcitable.

123 odine receptor PKA (S2808) or CaMKII (S2814) target sites failed to affect HR responses to isoprotere

124 Effective target sites fall within two domains, which are conserve

125 tially toxic, metal ion to its intracellular target sites following uptake.

126 The Si site offers a novel target site for allosteric inhibitors and a molecular ex

127 validate the Nef dimerization interface as a target site for antiretroviral drug development.

128 talk that was also previously suggested as a target site for chemically unrelated HA inhibitors.

129 the regulatory region of the SbCAD2 can be a target site for optimizing lignin modification in sorghu

130 s highlights the medial parietal cortex as a target site for transforming neural activity into contro

131 TGFbeta signaling genes that harbor putative target sites for miR-155.

132 of the SOX9 3'-UTR revealed highly conserved target sites for nine different miRNAs.

133 her mRNAs, suggesting that they provide more target sites for piRNAs to promote their preferential te

134 We reveal novel functional target sites for the microRNAs miR-181a, miR-30a, and mi

135 Notably, the predicted triple helix target sites for these HOTAIR domains were also enriched

136 artates 348, 387, and 390 were identified as target sites for this cleavage.

137 een PB1 and PB2, which could be important in targeting sites for anti-influenza intervention.

138 Through switching of target sites, FOXD3 modulates the developmental potentia

139 Furthermore, in conjunction with target site free drug concentrations and endogenous agon

140 The delivery of drugs to a target site frequently involves crossing biological barr

141 Amplification of the target site gene, 5-enolpyruvylshikimate-3-phosphate syn

142 PKA and CaMKII do not affect HR by a unique target site governing SR Ca(2+) uptake or release.

143 Recently, a large number of miRNA target sites have been discovered by newly emerged high-

144 loci simultaneously including potential off-target sites identified by ChIP-seq and by computational

145 Interaction between miR-650 and its target site in the 3' untranslated region was validated

146 istries and materials to deliver them to the target site in the body, at a therapeutic concentration,

147 uble drugs and small molecules to a specific target site in the body.

148 a previously uncharacterized phosphorylation target site in the Hec1 tail, as a critical Aurora A sub

149 forms a dimer that avidly binds a consensus target site in the promoters of regulated genes, and our

150 insertions as well as indel mutations at the target site in the rice genome.

151 f nuclear factor kappa B (NFkappaB) from DNA target sites in a process we have termed molecular strip

152 able and cost-effective coverage of specific target sites in all cells from a sample and in cases whe

153 le in recruitment of NuRD complexes to their target sites in chromatin.

154 To find their DNA target sites in complex solution environments containing

155 PmiR predicted more than 74.2% of true miRNA target sites in each dataset.

156 his interactome revealed an abundance of miR target sites in gene coding regions, including several s

157 HUSH recruits MORC2 to target sites in heterochromatin.

158 d translocation frequency to IGH and AID off-target sites in human chronic lymphocytic leukaemia and

159 allele using haplotype-specific CRISPR/Cas9 target sites in Huntington's disease (HD), a late-onset

160 hey can be programmed to cleave specific DNA target sites in living cells and organisms.

161 sion acting by direct base pairing to 3'-UTR target sites in messenger RNAs.

162 d reduces the accessibility of AGO2-miRNA to target sites in mRNAs.

163 Consequently, exact target sites in PPTg, possible DBS mechanisms, and poten

164 suring the DNA methylation state of multiple target sites in single cells, otherwise known as single-

165 ete hypothalamic arcuate neurons onto common target sites in the central nervous system has a fundame

166 Graphical visualization of on-target and off-target sites in the genome is provided for target valida

167 tely understood how proteins recognize their target sites in the genome.

168 SVP2 was found to bind to at least 297 target sites in the kiwifruit genome, and potentially mo

169 biology include two functionally independent target sites in the KRas 3'UTR and clinically significan

170 By identifying thousands of TERRA target sites in the mouse genome, we demonstrate that TE

171 that almost one-third of possible HLA-linked target sites in the transmitted virus Gag protein are al

172 how nucleosomes affect Cas9 cleavage at off-target sites in vitro, we used a single guide RNA (sgRNA

173 We sequence targeted sites in 632 founder mice and analyse 54 establ

174 performed gates in series on 48 individually targeted sites in a 40% full 5 by 5 by 5 three-dimension

175 binding sites in the spatial context of the target site indicates that the interactions between bind

176 chloride channel subunits tested, suggesting target site insensitivity may not be important in our ho

177 Once a target site is identified, the time between PFV strand t

178 ding of molecules, ions or proteins to small target sites is a generic step of cell activation.

179 The identification of microRNA (miRNA) target sites is fundamentally important for studying gen

180 The identification of microRNA (miRNA) target sites is important.

181 further show that cooperation between miRNA target sites is necessary for silencing in vivo in the C

182 ic feature of many conserved mammalian miRNA target sites is that an adenosine (A) nucleotide opposit

183 at on-target sites, but their impact at off-target sites is unknown.

184 s acetylation of histone H3 lysine 27 at its target sites, leading to robust transcriptional activati

185 r there are interspecific differences at the target-site level.

186 mainly to 3' UTRs, in Chlamydomonas utilized target sites lie predominantly within coding regions.

187 uclease-induced toxicity at both on- and off-target sites, likely due to DNA damage.

188 In this context we created four target site lines for RMCE and evaluated their fitness c

189 equires dynamic receptor binding to specific target sites located across the genome.

190 some species copy number variation (CNV) of target site loci (e.g. the Ace-1 target site of carbamat

191 aced short palindromic repeats (CRISPR)/Cas9 target sites, marks cells and enables the elucidation of

192 e evolution of mRNA sequences flanking miRNA-target sites may be impacted.

193 ening of target sequences through use of two target sites might ameliorate assay reliability because

194 cribed for PacI, implying that long A:T-rich target sites might display structural or dynamic behavio

195 end of coding regions, largely explained by target site motifs.

196 other weeds glyphosate resistance arose from target site mutation or target gene amplification, the r

197 es in this species is thought to be due to a target-site mutation conferring an amino acid substituti

198 Here we describe two resistance-associated target-site mutations that have synergistic and compensa

199 on (CNV) of target site loci (e.g. the Ace-1 target site of carbamate insecticides) or detoxification

200 he 3' untranslated region of CSR1 contains a target site of miR-650.

201 of Arabidopsis ARGONAUTE7 to a noncleavable target site of miR390 might directly hinder ribosome mov

202 untranslated region (UTR) that serves as the target site of the corresponding antitoxin sRNA.

203 or studying functional genomics of important target sites of anthelmintics have been restricted to Ca

204 nd that this genetic variation confounds the target sites of certain Cas endonucleases more than othe

205 Finally, we characterized the target sites of four IS families, confirming previous re

206 and local ablative therapies can be used to target sites of oligoprogression.

207 ed during erythropoiesis and that enrich for target sites of RNA-binding proteins that are specific t

208 A common denominator of the main target sites of the Foxb1-positive axons of the parvafox

209 Finally, all Rumi target sites of the human JAG1 are efficiently glucosyla

210 nodes, and intestinal mucosa served as major target sites of viral replication.

211 he voltage-gated sodium channel gene (Vgsc), target-site of pyrethroid and organochlorine insecticide

212 DNMT3A-CD or TET1-CD fusion proteins at the targeted site of the Ascl1 promoter.

213 locking Srebp proteolytic activation and has targeting sites of miR-33.

214 royed or created novel illegitimate microRNA target sites; of them, 78 SNPs explained significant var

215 ained their ability to spread to intron-free target sites, often assisted by intron-encoded endonucle

216 nalyzed its association with an unmethylated target site on fluorescence-labeled DNA in the presence

217 Anti-AD-5 MAbs share a target site on gB, despite originating from different, h

218 which binds strongly to Myc but not MycN, to target sites on chromatin.

219 by directing other molecules to reach their target sites on collagens.

220 sembles linker histone and thereby binds its target sites on nucleosomes and in compacted chromatin.

221 matically evaluated validated PKA and CaMKII target sites on phospholamban and the ryanodine receptor

222 ggers Mrr activity by creating high affinity target sites on the chromosome, which pull the equilibri

223 Interaction of miR-138 with a predicted targeting site on the osteocalcin (OC) promoter resulted

224 ociated with alterations to the insecticidal target-site or with gene expression variation at loci in

225 The effect of target site orientation (head-to-head or head-to-tail) o

226 We examined the potential off-target sites (OTS) by whole-genome high-throughput seque

227 nce, we still cannot reliably identify miRNA target sites, partially due to our limited understanding

228 tics of miRNA target sites and improve miRNA target site prediction methods.

229 of computational methods available for miRNA target site prediction.

230 veloped an approach called TarPmiR for miRNA target site prediction.

231 w L1 insertions bore structural hallmarks of target-site primed reverse transcription (TPRT) and mobi

232 e concatemeric IDLV genomic structure at the target site, probably resulted from recombination of the

233 riched in NGG sites, a sequence required for target site recognition by Streptococcus pyogenes Cas9.

234 In the absence of LMO2, the target site recognition of TAL1 is impaired.

235 r, questions remain about the specificity of target site recognition.

236 similar function by promoting Flp binding to target sites, reducing non-productive binding and/or enh

237 r creates a cycle of cleavage, ligation, and target site regeneration that persists until sufficient

238 Guidance of axons to their proper synaptic target sites requires spatially and temporally precise m

239 ld grasses is widespread in the UK, with non-target site resistance (NTSR) to multiple chemistries be

240 Non-target-site resistance (NTSR) to herbicides that disrupt

241 GhMYB25-like-sgRNA1 and GhMYB25-like-sgRNA2 target site respectively.

242 mHtt aggregates reduce transcription factor target site sampling frequency and impair critical gene

243 ique for directly and specifically measuring target site search by DNA-binding proteins via intersegm

244 HCV target site selection follows canonical miRNA rules, but

245 nces, but conserved principles guiding their target site selection have not been established.

246 roRNAs has the potential to cause a shift in target site selection.

247 loop were tested for effects on integration target site selection.

248 that editing results in a complete switch of target site selection.

249 sults provide insights into the mechanism of target site selection.

250 Target-site selection by retroviral integrase (IN) prote

251 fy new dynamic IN functions and suggest that target site-selection limits retroviral integration.

252 nduced phosphorylation of PIMT at the ERK1/2 target site Ser(298).

253 The list of off-target sites showing activity in cells was influenced by

254 ionship between miRNAs and target abundance, target-site spacing, and affinity requirements for ceRNA

255 te, effectively deleting the majority of the target site such that it cannot be regenerated.

256 h an insertion at an unlinked, independently targeted site, suggesting enrichment of a sub-population

257 cid treatment, Hoxa1 is rapidly recruited to target sites that are associated with genes involved in

258 tes in the C-terminal region of BRM at SnRK2 target sites that are evolutionarily conserved.

259 However, Cas9 can cleave off-target sites that are not fully complementary to the gui

260 1029 SNPs in the targets, 3 were located in target sites that could change the binding affinity of P

261 eriments show that high levels of Myc invade target sites that lack consensus E-boxes in a complex wi

262 ion, this enzyme produces DNA lesions at off-target sites that lead to mutations and chromosome trans

263 tic mutations in microRNAs (miRNA) and their target sites that potentially alter the interactions bet

264 interlobe groove of NCT, emerges as an allo-targeting site that would impact the coupling between HL

265 has been shown to bind and cleave DNA at off-target sites, the field of Cas9 specificity is rapidly p

266 Even for atypical, repetitive target sites, the vast majority of off-target mutations

267 Once accumulated at the targeting site, the CPP modified drug is released from t

268 bond hydrolysis within or close to their DNA target sites, they form different oligomeric assemblies

269 entering the cell should not diffuse to the target site through the cytosol, as this would potential

270 ly defined by exact sequence homology to the target site, thus unintended off-targets might additiona

271 ntains guide regions that base-pair with the target site to select the single nucleotide to be modifi

272 nserted reporters to track tropism, microRNA target sites to restrict tropism, or barcodes to assess

273 C) and benign liver tissue to identify miRNA target sites transcriptome-wide in two species.

274 Out of 15 predicated off-target sites, tru-RGNs showed significantly decreased fr

275 However, unlike metazoan miRNA where target site utilization localizes mainly to 3' UTRs, in

276 n, which occurs when introns spread to empty target sites via homologous recombination.

277 By editing an endogenous target site, we demonstrate that 3' pairing determines t

278 ieving recombination at arbitrary asymmetric target sites, we have broken the symmetry of the Cre tet

279 To identify endogenous miRNA-target sites, we isolated AGO-bound RNAs from Caenorhabd

280 al new scaffolds that bind to the allosteric target site were generated and one example was validated

281 hromosomal locations, cis-elements and miRNA target sites were comprehensively investigated.

282 Several target sites were validated by means of in vitro lucifer

283 These target sites were, in most cases, not associated with a

284 ate flanked by homology DNA fragments to the target site, were demonstrated to generate precise gene

285 HT6R (Ki = 2.04 nM) and selectivity over 100 target sites which include receptors, enzymes, peptides,

286 tion between the transposase and a potential target site, which may be how other DNA binding proteins

287 altered on-target sites and novel potent off-target sites, which can predispose patients to treatment

288 was evaluated by sequencing two putative off-target sites, which have 3 and 1 mismatched nucleotides

289 as observed at both target sites and one off-target site, while no cleavage activity could be detecte

290 pulations of individuals, most candidate off-target sites will be rare, underscoring the need for pre

291 ur different datasets, CCmiR predicted miRNA target sites with a high recall and a reasonable precisi

292 -avebetrin allow for selective addressing of target sites with different integrin expression levels b

293 prehensive biochemical identification of off-target sites with independent cell-based measurements of

294 nucleases have also been shown to cut at off-target sites with mutagenic consequences.

295 possessing better discrimination against off-target sites with non-canonical NAG and NGA PAMs and/or

296 Endothelial cells at target sites with reduced Flt1 and/or elevated protrusiv

297 entify Ser-45 and Ser-46 of Dgk1 as the CKII target sites, with Ser-46 being the major phosphorylatio

298 Howardula rRNA in vitro at the canonical RIP target site within the alpha-sarcin/ricin loop (SRL) of

299 te gene expression through interactions with target sites within mRNAs, leading to enhanced degradati

300 igh antineoplastic drug concentration at the target site without damaging healthy tissues.