ライフサイエンスコーパス: tasiRNA

1 tasiRNAs were generated in the transgenic seedlings, and

2 Mismatches at that position abolished tasiRNA formation, but mismatches at the 5' end of miR17

3 cs of the target transcripts may also affect tasiRNA production.

4 In contrast, miRNA and tasiRNA sequences were conserved between species, but th

5 L4 showed functional redundancy in siRNA and tasiRNA production and in the establishment and maintena

6 , the phasing patterns of known tasiRNAs and tasiRNA-like loci from across the Arabidopsis genome wer

7 approaches for identification of miRNAs and tasiRNAs and their targets.

8 fering RNA and microRNA, recently defined as tasiRNA.

9 Our data reveal complex tasiRNA-based regulation of NB-LRRs that potentially evo

10 tor protein pair to function at two distinct tasiRNA biogenesis steps.

11 slicer activity produce abundant siRNAs from tasiRNA loci in vivo.

12 ion and specific sequence complementarity in tasiRNA formation, providing new insights into tasiRNA b

13 siRNA formation, providing new insights into tasiRNA biogenesis as well as a strategy for improving t

14 on processing the 3' cleavage products into tasiRNAs.

15 al approaches, the phasing patterns of known tasiRNAs and tasiRNA-like loci from across the Arabidops

16 56 and miR390 directly target a single major tasiRNA primary transcript.

17 re we present SoMART, a web server for miRNA/tasiRNA analysis resources and tools, which is designed

18 A accumulation and decreased accumulation of tasiRNA targets.

19 The identification of tasiRNA-ARF as a low-abundance, previously uncharacteriz

20 ), one-hit or two-hit, or even two layers of tasiRNA-TASL interactions.

21 d tasiRNA accumulation, and reduced level of tasiRNA targets.

22 option may be related to unique qualities of tasiRNA-mediated regulation.

23 g is solely required for phase definition of tasiRNAs, and they strongly support recruitment of RDR6

24 In tex1 plants, the levels of tasiRNAs are reduced, while miR173 levels are decreased

25 or transgene silencing and the production of tasiRNAs.

26 e stop codons caused a dramatic reduction of tasiRNAs and over-accumulation of 3' cleavage products,

27 phased inflorescence RNAs resembles that of tasiRNAs and raise the possibility that these novel smal

28 ite location and sequence complementarity on tasiRNA formation.

29 ' for identifying miRNA precursors (MIRs) or tasiRNA precursor (TASs) of input sRNAs, and 'sRNA mappe

30 , we identified 79 conserved miRNA:target or tasiRNA:target interactions, of which eight were further

31 who are interested in identifying miRNAs or tasiRNAs that potentially regulate genes of interest.

32 itial targeting by one or multiple miRNAs or tasiRNAs, the most conspicuous example of which was an e

33 ed as trans-acting small interfering RNAs or tasiRNAs.

34 E6 pathway, and sets the register for phased tasiRNA formation by DICER-LIKE4.

35 RNA pathway, that of miR173-TAS1/2, produces tasiRNAs regulating a set of pentatricopeptide repeat (P

36 3 dcl4 triple mutant showed markedly reduced tasiRNA and siRNA production and indicated that DCL1, in

37 The first reported tasiRNA pathway, that of miR173-TAS1/2, produces tasiRNA

38 t unique trans-acting small interfering RNA (tasiRNA) biogenesis profiles and target specificities.

39 rans-acting or phased small-interfering RNA (tasiRNA/phasiRNAs).

40 Trans-acting small interfering RNAs (tasiRNAs) are a major class of small RNAs performing ess

41 tion of trans-acting small interfering RNAs (tasiRNAs) dependent upon a second ancient microRNA, miR3

42 As) and trans-acting small interfering RNAs (tasiRNAs) play important roles in a variety of biologica

43 As) and trans-acting small interfering RNAs (tasiRNAs) regulate mRNA stability and translation, and s

44 tion of trans-acting short interfering RNAs (tasiRNAs).

45 fferent plant families, have evolved similar tasiRNA pathways to initiate phased small interfering RN

46 Transacting siRNA (tasiRNA) biogenesis in Arabidopsis is initiated by micro

47 mary transcripts into the transacting siRNA (tasiRNA) biogenesis pathway involving RNA-DEPENDENT RNA

48 but less conserved TAS3 trans-acting siRNAs (tasiRNAs) in addition to tasiR-ARFs, with expanded poten

49 Trans-acting siRNAs (tasiRNAs) negatively regulate target transcripts and are

50 ins several families of trans-acting siRNAs (tasiRNAs) that form in 21-nucleotide phased arrays throu

51 atin-associated siRNAs, trans-acting siRNAs (tasiRNAs), and natural antisense transcript (NAT)-associ

52 ring RNAs (siRNAs), and trans-acting siRNAs (tasiRNAs), control gene expression and epigenetic regula

53 r biogenesis of phased, trans-acting siRNAs (tasiRNAs), whose cleaved precursor fragments are convert

54 RNAs are processed into trans-acting siRNAs (tasiRNAs).

55 ocus was modified to produce synthetic (syn) tasiRNA to target an endogenous transcript encoding PHYT

56 Several amiRNA and syn-tasiRNA sequences designed to target one or more endogen

57 s in its ability to initiate TAS1c-based syn-tasiRNA formation.

58 ility to generate amiRNA and multiplexed syn-tasiRNA constructs for efficient gene silencing in Arabi

59 BsaI/ccdB vectors for amiRNA or syn-tasiRNA cloning and expression contain a modified versio

60 Current methods to generate amiRNA or syn-tasiRNA constructs are not well adapted for cost-effecti

61 amiRNA or syn-tasiRNA inserts resulting from the annealing of two over

62 tions, P-SAMS amiRNA Designer and P-SAMS syn-tasiRNA Designer.

63 evels of accurately processed amiRNAs or syn-tasiRNAs, and (3) had reduced levels of the correspondin

64 tic trans-acting small interfering RNAs (syn-tasiRNAs) are used for small RNA-based, specific gene si

65 tic trans-acting small interfering RNAs (syn-tasiRNAs) for efficient and specific targeted gene silen

66 with ARGONAUTE 1 (AGO1) during TAS1 and TAS2 tasiRNA formation, and we provide data indicating that t

67 dopsis mutants with specific defects in TAS3 tasiRNA biogenesis or function was done.

68 In the case of TAS3 tasiRNA formation, ARGONAUTE7 (AGO7)-miR390 complexes in

69 0/miR390* duplex and subsequent reduced TAS3 tasiRNA levels.

70 The TAS3 tasiRNA pathway has been coopted to regulate diverse dev

71 is process, we explored cooption of the TAS3 tasiRNA pathway in the moss Physcomitrella patens.

72 ing and experimentation, we demonstrate that tasiRNA regulation confers sensitivity and robustness on

73 It was found that tasiRNAs were most efficiently produced when the miR173

74 ing that these genes may be regulated by the tasiRNA pathway.

75 have contributed to repeated cooption of the tasiRNA-ARF module during evolution.

76 miR173 in routing of transcripts through the tasiRNA pathway.

77 on 21 nt small RNAs, can efficiently trigger tasiRNA formation.

78 6 function causes increased miR390-triggered tasiRNA accumulation and decreased accumulation of tasiR

79 n of gametophores, elevated miR390-triggered tasiRNA accumulation, and reduced level of tasiRNA targe

80 SOR OF GENE SILENCING3, but unlike wild-type tasiRNAs, they are unphased.

81 efficiency of RNA interference (RNAi) using tasiRNAs.

82 ed sORF of TAS3a linked its translation with tasiRNA biogenesis.

83 dopsis thaliana TAS1 and TAS2 families yield tasiRNA that form through miR173-guided initiation-cleav