ライフサイエンスコーパス: taste cell

1 t and receive synaptic contact from Type III taste cells.

2 ed changes in C(m) in the different types of taste cells.

3 of which is transduced by a separate set of taste cells.

4 on conductance specific to PKD2L1-expressing taste cells.

5 ting cAMP alters Ca(2+) levels in identified taste cells.

6 ngs food-related chemicals into contact with taste cells.

7 s as well as physiologic studies of isolated taste cells.

8 otassium exchangers (NCKXs) are expressed in taste cells.

9 s with the signalling mechanisms used by the taste cells.

10 s with the signalling mechanisms used by the taste cells.

11 localized to the apical tips of a subset of taste cells.

12 receptors expressed in different subsets of taste cells.

13 le one of these, ROMK2, in a subset of mouse taste cells.

14 secreted from isolated mouse taste buds and taste cells.

15 in-1-LIR are present in different subsets of taste cells.

16 e qualities have been recorded in individual taste cells.

17 receptor signaling and membrane potential in taste cells.

18 o acid decarboxylase (AADC) are expressed in taste cells.

19 dense-cored vesicles previously seen in some taste cells.

20 n detected immunocytochemically in mammalian taste cells.

21 y compose a subpopulation of acid-responsive taste cells.

22 xplain the taste responses observed in mouse taste cells.

23 d in taste tissue and in Ggamma13-expressing taste cells.

24 gal) and trkB colocalize, mainly in type III taste cells.

25 trophin (NT)-3- nor trkC-LIR was detected in taste cells.

26 of electrophysiological studies performed on taste cells.

27 eptor (IP3R3), a taste-signaling molecule in taste cells.

28 sm contributes to salt responses in type III taste cells.

29 ific G-protein-coupled membrane receptors on taste cells.

30 esent in differentiated type II and type III taste cells.

31 changes in pH and [Ca2+]i simultaneously in taste cells.

32 th blood group H antigen, a marker of type I taste cells.

33 to be detected by T1r receptors expressed in taste cells.

34 esponsive receptors and/or pathways exist in taste cells.

35 n-induced Ca2+ entry into a select subset of taste cells.

36 ong slender taste cells, as well as pyriform taste cells.

37 ls compose subpopulations of acid-responsive taste cells.

38 different subpopulations of bitter-sensitive taste cells.

39 population of broadly tuned bitter-sensitive taste cells.

40 ovilli, which are characteristics of Type II taste cells.

41 croM) did not increase the number of stained taste cells.

42 gemmal nerve processes and a small subset of taste cells.

43 iosynthesizing enzyme of endocannabinoids in taste cells.

44 lycerol lipase alpha (DAGLalpha)) of 2-AG in taste cells.

45 vation of Y2 receptors localized apically in taste cells.

46 activation of its high affinity receptor in taste cells.

47 eoside did not affect Presynaptic (type III) taste cells.

48 m5) and type III (e.g. Pkd2l1, Ncam, Snap25) taste cells.

49 s calcium mobilization in Receptor (Type II) taste cells.

50 to the postnatal expression of gustducin in taste cells.

51 nel (Kir) 6.1] were expressed selectively in taste cells.

52 re expressed preferentially in T1r3-positive taste cells.

53 rtion of K(+) channels in the T1r3 subset of taste cells.

54 owed that a subset of Presynaptic (Type III) taste cells (53%) responded to 0.1 mum CGRP with an incr

55 substrates of taste behaviors, we monitored taste cell activity in vivo with the genetically encoded

56 lace at the microvilli on the apical side of taste cells after diffusion of the molecules through the

57 lecular receptive range (MRR); some of these taste cells also contain two signaling pathways with dis

58 the overwhelming majority of T1r3-expressing taste cells also express SUR1, and vice versa, it is lik

59 using a reporter mouse strain, we show that taste cells also express the anti-inflammatory cytokine

60 tion involves communication between Type III taste cells and 5-HT3 -expressing afferent nerve fibers

61 y longer and narrower than FITBs, with fewer taste cells and a smaller nerve plexus.

62 urotransmitters to communicate with adjacent taste cells and afferent nerve fibers.

63 g, we identified AI salt-responsive type III taste cells and demonstrated that they compose a subpopu

64 , specifically free fatty acids, to activate taste cells and elicit behavioral responses consistent w

65 acid, linoleic acid (LA), depolarizes mouse taste cells and elicits a robust intracellular calcium r

66 e and suggests subsequent synaptic spread to taste cells and epithelial cells via peripheral synapses

67 addition, the immunocytochemical profile of taste cells and gustatory behavior were examined in wild

68 involved in sugar and amino acid sensing in taste cells and in the gastrointestinal tract.

69 in the apical membrane of PKD2L1-expressing taste cells and is not affected by targeted deletion of

70 s well as by GAD67 in presynaptic (type III) taste cells and is stored in both those two cell types.

71 In taste cells and macrophages, CD36 signaling was recently

72 The ultrastructure of contacts between taste cells and nerve fibers is also normal in the DKO m

73 we did not observe synapses between type II taste cells and nerve fibers.

74 Synaptic contacts between taste cells and nerve processes have been found to exist

75 tions between the plasma membrane of type II taste cells and nerve processes.

76 as secreted only from presynaptic (type III) taste cells and not receptor (type II) cells.

77 heterogeneous population of bitter-sensitive taste cells and signaling pathways within this insect fa

78 s (SCCs) have a structure similar to lingual taste cells and strongly express alpha-gustducin.

79 ial role in fatty acid transduction in mouse taste cells and suggest that fatty acids are capable of

80 ne was mediated directly by the desensitized taste cells and that the adapted aversive response did n

81 ncreases in intracellular Ca(2+) in isolated taste cells and that the source of the Ca(2+) is release

82 ecific transfer of taste information between taste cells and the central nervous system.

83 citatory responses from the bitter-sensitive taste cells and then used these responses to formulate p

84 Using calcium imaging on single isolated taste cells and with biosensor cells to identify neurotr

85 markedly reduced cytokeratin 8 expression in taste cells, and a high incidence of a filiform-like spi

86 bud placodes, fungiform papillae, and mature taste cells, and low levels in filiform papillae.

87 nsory neurons, solitary chemoreceptor cells, taste cells, and Merkel cells.

88 evalence of poorly differentiated or missing taste cells, and the incidence of ectopic filiform-like

89 Although gustducin-immunoreactive taste cells appear similar in overall shape to the pyrif

90 Taste cells are continuously renewed throughout life via

91 Moreover, they show that individual taste cells are dedicated to the transduction of only on

92 the synapses associated with syntaxin-1-LIR taste cells are from type III cells onto nerve processes

93 transgenic mice in which PLCbeta2-expressing taste cells are labeled with green fluorescent protein.

94 As placodally derived taste cells are lost, we used Wnt1Cre mice to show that

95 B-LIR, and p75-LIR elongated, differentiated taste cells are present within all lingual taste buds, w

96 within taste buds, and like epidermal cells, taste cells are regularly replaced throughout adult life

97 bpopulation of serotonin-immunoreactive (IR) taste cells are related by lineage to a restricted set o

98 These studies demonstrate that taste cells are selectively tuned to different taste mod

99 These studies demonstrate that taste cells are tuned by taste category and are hardwire

100 ng pathways within the same bitter-sensitive taste cell (aristolochic acid).

101 buds: GABA is synthesized by GAD65 in type I taste cells as well as by GAD67 in presynaptic (type III

102 , beta-gal (BDNF) is present in long slender taste cells, as well as pyriform taste cells.

103 ence of a reduced number of bitter-activated taste cells, as well as reduced sensitivity.

104 ify genes that were selectively expressed in taste cells at different maturation stages.

105 urs in anteriorly placed taste buds, however taste cells at the back of the tongue respond to umami c

106 ype I (blood group H antigen immunoreactive) taste cells but is present in differentiated type II and

107 cocious differentiation of Type I glial-like taste cells, but not other taste cell types.

108 t to gate TRPM5-dependent currents in intact taste cells, but only intracellular Ca2+ is able to acti

109 We recorded activation of taste cells by bitter stimuli using Ca2+ imaging in ling

110 e buds without affecting the excitability of taste cells by taste stimuli.

111 y, acid sensitivity is not conferred on sour taste cells by the specific expression of Kir2.1, but by

112 ing pathway within the same bitter-sensitive taste cell (caffeine).

113 In response to inflammatory challenges, taste cells can increase IL-10 expression both in vivo a

114 Similarly, Type II taste cells come in at least two varieties: those immuno

115 ificantly larger and have a larger number of taste cells compared with controls.

116 We isolated mouse taste buds and taste cells, conducted functional imaging using Fura-2,

117 This communication between taste cells could represent a potential convergence of t

118 data suggest a model where continued natural taste cell death, paired with temporary interruption of

119 These are all characteristics of Type III taste cells described previously in rabbits.

120 In bdnf(-/-) mice taste cell development failed because of sparse gustator

121 ts taste perception without interfering with taste cell development or integrity.

122 ntify multiple signaling pathways underlying taste cell differentiation and taste stem/progenitor cel

123 Strikingly, placodally descendant taste cells disappear early in adult life.

124 cal features, that both type II and type III taste cells display synaptobrevin-2-LIR.

125 e populations of AI salt-responsive type III taste cells distinguished by their sensitivity to anion

126 approximately 27% of the synaptobrevin-2-LIR taste cells do not display IP3R3-LIR.

127 uggesting that ACh is normally released from taste cells during taste stimulation.

128 croscopy also showed the calcium activity of taste cells elicited by small-sized tastants in the bloo

129 PGP 9.5-immunoreactive taste cells exhibit two morphological varieties.

130 on of the whole taste bud, only a select few taste cells exhibited Ca2+ responses.

131 nscription (RT)-PCR, we show that individual taste cells express either phospholipase C beta2 (PLCbet

132 aste; our starting point was to determine if taste cells express glucose transporters (GLUTs) and met

133 All IP3R3-LIR taste cells express synaptobrevin-2-LIR.

134 These taste cells express the epithelial sodium channel ENaC,

135 ning showed that nearly all rodent and human taste cells express this channel.

136 Alpha-gustducin, a taste cell-expressed G-protein alpha subunit closely rel

137 Taste cell-expressed glucose sensors and K(ATP) may serv

138 A recent study showed that taste cells expressing bitter-responsive taste receptors

139 nt protein, we previously reported that sour taste cells from circumvallate papillae in the posterior

140 from DKO mice, as from wild-type (WT) mice, taste cells from DKO mice fail to release ATP when stimu

141 nion sodium salts, respectively) in isolated taste cells from mouse circumvallate papillae.

142 the current is present in PKD2L1-expressing taste cells from mouse circumvallate, foliate, and fungi

143 ells isolated from TRPM5 knockout mice or in taste cells from wild type mice where current through TR

144 This demonstrates a direct taste cell function for BDNF.

145 however, the molecular mechanisms governing taste cell generation are not well understood.

146 1) different populations of bitter-sensitive taste cells (Grindelia extract and Canna extract) or (2)

147 The other type of PGP 9.5-immunoreactive taste cell has a large round nucleus and the apical end

148 Each bilateral pair of bitter-sensitive taste cells has a different molecular receptive range (M

149 proton conductance that is specific to sour taste cells has been shown to be the initial event in so

150 ha-gustducin in bitter taste transduction in taste cells has not been demonstrated in situ at the cel

151 els encoded by the TRPM5 gene, we found that taste cells have a second type of Ca2+-activated nonsele

152 The serotonin-immunoreactive taste cells have an invaginated nucleus, synaptic contac

153 Gustatory receptors and peripheral taste cells have been identified in flies and mammals, r

154 Notably, mature taste cells have life spans of only 5-20 days and, conse

155 expressing these molecules are distinct from taste cells having receptors for bitter, sweet, or umami

156 NGF, pro-NGF, and trkA coexist in type II taste cells, i.e., those expressing phospholipase Cbeta2

157 t that fatty acids are capable of activating taste cells in a manner consistent with other GPCR-media

158 hypothesis of purinergic signalling between taste cells in a more integral preparation.

159 we undertook an immunohistochemical study of taste cells in BDNF(LacZ) gene targeted "knock-in" adult

160 The T1r3 gene is expressed in a subset of taste cells in circumvallate, foliate and fungiform tast

161 ses in cells isolated from taste buds and in taste cells in lingual slices to acetic acid titrated to

162 We show that PYY is expressed in subsets of taste cells in murine taste buds.

163 aste qualities by distinct subpopulations of taste cells in peripheral gustatory sensory organs in mi

164 -LIR) is present in approximately 35% of the taste cells in rat circumvallate taste buds.

165 sent in the morphologically defined Type III taste cells in rats.

166 act tongue mucosa and functional activity of taste cells in response to topically administered tastan

167 Cell lineage analysis of serotonin-IR taste cells in such mixed taste buds suggests that this

168 Whereas 82% of the taste cells in the clOFC respond to glucose, in the mOFC

169 f carbonation as well as the contribution of taste cells in the orosensory response to CO2.

170 To examine the role of PKD2L1-expressing taste cells in vivo, we engineered mice with targeted ge

171 Activation of these taste cells, in turn, engages neural circuits in the cen

172 the mechanisms underlying integration of new taste cells into the taste bud.

173 hological taste cell types, but the type III taste cell is the only cell type that has synapses onto

174 is constitutively open, the cytosol of sour taste cells is acidified.

175 In this study, we use taste cells isolated from mouse vallate and foliate papi

176 Nevertheless, some taste cells lacking alpha-gustducin responded to bitter

177 s reveal that acids activate a unique set of taste cells largely dedicated to sour taste, and they in

178 Moreover, siRNA knockdown of WT1 in cultured taste cells leads to a reduction in the expression of Le

179 ning with antibodies against type II and III taste cell markers validated the presence of KCNQ1 in th

180 multiple progenitors, the different types of taste cells may represent distinct lineages.

181 Modulating GLP-1 secretion in gut "taste cells" may provide an important treatment for obes

182 Modulating hormone secretion from gut "taste cells" may provide novel treatments for obesity, d

183 In contrast, pharyngeal taste cells mediate the egg-laying attraction to lobelin

184 expressed in the alpha-gustducin lineage of taste cells mediate these responses.

185 roton conductance to directly depolarize the taste cell membrane.

186 onium, which interacts with K(+) channels in taste cells, most likely binds to and blocks Kir6.2.

187 ors are specified and produce differentiated taste cells normally, in the absence of gustatory nerve

188 ease after development but continues in some taste cells of adult mice.

189 The identification in sour taste cells of an acid-sensitive K(+) channel suggests a

190 aled significantly stronger BDNF labeling in taste cells of high BDNF-expressing mouse lines compared

191 e responses to umami tastants persist in the taste cells of T1R3-knockout mice.

192 We recorded whole-cell responses of 120 taste cells of the rat fungiform papillae and soft palat

193 rs (VPAC1, VPAC2) were identified in type II taste cells of the taste bud, and VIP knockout mice exhi

194 se structures in the tongue, neuroepithelial taste cells of the taste bud, and, possibly, epithelial

195 glucose through the same mechanisms used by taste cells of the tongue.

196 on and that sugar-sensing inhibition affects taste cells on the proboscis and the legs.

197 Sour taste is detected by a subset of taste cells on the tongue and palate epithelium that res

198 could underlie AI salt responses in type III taste cells, one of which may contribute to the anion ef

199 mediated by three pairs of bitter-sensitive taste cells: one responds vigorously to aristolochic aci

200 All of the synapses that we observed from taste cells onto nerve processes express synaptobrevin-2

201 ggest that epithelial cells, neuroepithelial taste cells, or olfactory sensory neurons at chemosensor

202 Sweet taste cells play critical roles in food selection and fe

203 Previous studies have identified one taste cell population marked by the gustatory receptor g

204 p75-LIR also is present in both BDNF and NGF taste cell populations.

205 Taste cells possess vesicles containing various neurotra

206 To determine which types of taste cells produce BDNF, we undertook an immunohistoche

207 and Hh signaling pathways are necessary for taste cell proliferation, differentiation and cell fate

208 e data define a functional signature for the taste cell proton current and indicate that its expressi

209 In contrast, Receptor (Type II) taste cells rarely (4%) responded to 0.1 mum CGRP.

210 o molecularly distinct functional classes of taste cells: receptor cells and synapse-forming cells.

211 ry and inhibitory effects often differs when taste cell recording changes from the NST to the PBN.

212 Type III taste cells release 5-HT directly in response to acidic

213 In response to taste stimuli, taste cells release ATP, activating purinergic receptors

214 d on cell processes that often envelop other taste cells, reminiscent of type I cells.

215 is in part responsible for the dependence of taste cell renewal on gustatory innervation, neurotrophi

216 de a local supply of Hh ligand that supports taste cell renewal.

217 re used to discriminate type II and type III taste cells, respectively.

218 onin (5HT) as markers of type I, II, and III taste cells, respectively.

219 For instance, whether and how individual taste cells respond to multiple chemical stimuli is stil

220 Calcium imaging revealed that isolated taste cells responded with a transient elevation of cyto

221 onded to multiple taste qualities, with some taste cells responding to both appetitive ("sweet") and

222 T1R3 as a coreceptor in taste cells responds to sweet compounds and amino acids;

223 Inosine monophosphate enhanced taste cell responses to L-glutamate, a characteristic fe

224 tively inhibits behavioural, taste nerve and taste cell responses to sweet compounds.

225 Approximately 5% of taste cells selectively responded to L-glutamate when it

226 identifying their patterns of expression in taste cells sheds light on coding of taste information b

227 VIP knockout mouse taste cells show a significant decrease in leptin recept

228 The results demonstrate that only type III taste cells show significant depolarization-induced incr

229 TrkB transcripts in taste buds and elevated taste cell-specific TrkB phosphorylation in response to

230 TPs, many (66%) AI salt-responsive type III taste cells still exhibited the anion effect, demonstrat

231 comprehensive map of gene expression for all taste cell subpopulations and will be particularly relev

232 localization suggest the presence of several taste cell subtypes.

233 These results support the proposition that taste cell synapses use classical SNARE machinery such a

234 We hypothesize that taste cell synapses utilize the SNARE protein machinery

235 ol synaptic vesicle fusion and exocytosis at taste cell synapses.

236 is associated with synaptic vesicles at rat taste cell synapses.

237 icle docking and neurotransmitter release at taste cell synapses.

238 ary, we postulate that aminergic presynaptic taste cells synthesize only 5-HT, whereas NE (perhaps se

239 sion in two distinct subpopulations of mouse taste cells: Tas1r3-expressing type II cells and physiol

240 Isolated taste cells, taste buds and strips of lingual tissue fro

241 salty, sour, umami) are sensed by dedicated taste cells (TCs) that relay quality information to gust

242 Z) mice indicate that BDNF is not present in taste cells that are younger than 3 days postmitotic.

243 In Drosophila, taste cells that contain the Gr5a receptor are necessary

244 ession is mostly restricted to the subset of taste cells that detect sour.

245 and L-amino acids is exclusively mediated by taste cells that express one or pair-wise combinations o

246 blished that sour is detected by a subset of taste cells that express the TRP channel PKD2L1 and its

247 cAMP and Ca(2+) signalling in a subclass of taste cells that form synapses with gustatory fibres and

248 hannels and calcium regulatory mechanisms in taste cells that functions to keep cytosolic calcium lev

249 that a constitutive calcium influx exists in taste cells that is regulated by mitochondrial calcium t

250 t has approximately eight bilateral pairs of taste cells that respond selectively to bitter taste sti

251 In taste cells that responded to multiple stimuli, there we

252 not well understood, and the identity of the taste cells that secrete NE is not known.

253 e information in the taste bud, resulting in taste cells that would respond broadly to multiple taste

254 s review discusses the functional classes of taste cells, their cell biology, and current thinking on

255 desensitized all of the caffeine-responsive taste cells to caffeine but not to aristolochic acid.

256 llular stores while other stimuli depolarize taste cells to cause calcium influx through voltage-gate

257 ough the apical pore, and allowing excitable taste cells to maintain a hyperpolarized resting membran

258 ATP further stimulates other taste cells to release a second transmitter, serotonin.

259 amines in taste, we evaluated the ability of taste cells to synthesize, transport, and package 5-HT a

260 How taste cells transduce sour taste is controversial becaus

261 In taste cells, Trpm5 was coexpressed with taste-signaling

262 taste bud, and resolve the paradox of broad taste cell tuning despite mutually exclusive receptor ex

263 cellular and molecular mechanisms governing taste cell turnover.

264 Which taste cell type(s) (Type I/glial-like cells, Type II/rec

265 ma receptor IFNGR1, are coexpressed with the taste cell-type markers neuronal cell adhesion molecule

266 nts in the three principal elongate types of taste cells: type I, II, and III.

267 To determine which of the three taste cell types expresses this enzyme, double-label ass

268 unohistochemistry using markers of different taste cell types in brain-derived neurotrophic factor (B

269 To identify the taste cell types that express proteins involved in PLC s

270 ch give rise to at least two different adult taste cell types, but do not contribute to taste papilla

271 lian buds contain a variety of morphological taste cell types, but the type III taste cell is the onl

272 The ectopic buds are composed of all taste cell types, including support cells and detectors

273 To identify taste cell types, mice expressing green fluorescent prot

274 Type I glial-like taste cells, but not other taste cell types.

275 calizes with markers of type II and type III taste cells: ubiquitin carboxyl terminal hydrolase (PGP

276 Taste cells undergo constant turnover throughout life; h

277 To investigate whether taste cells undergo regulated exocytosis, we used the co

278 Taste cells use multiple signalling mechanisms to genera

279 1 as the acid-sensitive K(+) channel in sour taste cells using pharmacological and RNA expression pro

280 stinct functions for PYY produced locally in taste cells vs. that circulating systemically.

281 Glutamate-stimulated cobalt uptake in taste cells was antagonized by the non-NMDA receptor ant

282 the total outward current of mouse fungiform taste cells was composed of K(ATP) channels.

283 In contrast, the number of differentiated taste cells was not significantly reduced until 7 dpi.

284 ing Fura-2 imaging of isolated mouse vallate taste cells, we explored how elevating cAMP alters Ca(2+

285 on studies on isolated taste buds and single taste cells, we have postulated that ATP secreted from r

286 Using calcium imaging of isolated mouse taste cells, we identify two separate populations of AI

287 e transcriptase (RT)-PCR on isolated vallate taste cells, we show that many Receptor cells express th

288 differentiate into different types of mature taste cells, we sought to identify genes that were selec

289 Most bitter-responsive taste cells were activated by only one out of five compo

290 clusively by three pairs of bitter-sensitive taste cell, which are located in the medial, lateral, an

291 -10 is produced by only a specific subset of taste cells, which are different from the TNF-producing

292 ation generates excitatory responses in sour taste cells, which can be attributed to block of a resti

293 TRPM5 is believed to depolarize taste cells, which leads to the release of ATP, which ac

294 logic responses to umami stimuli in isolated taste cells, which suggests that cAMP may have a modulat

295 ld elicit depolarization of sweet-responsive taste cells, which would transmit their signal to gustat

296 examined was heterogeneously distributed in taste cells with notably more GABA positive cells presen

297 Previous studies have shown that taste cells with synapses display SNAP-25- and synaptobr

298 Because this SNAP-25 antibody can label taste cells with synapses, it may also serve as a useful

299 at immunoreactivity to SNAP-25 is present in taste cells with synapses.

300 express synaptobrevin-2-LIR, as well as some taste cells without synapses.