ライフサイエンスコーパス: taurocholate

1 etrograde pancreatic duct infusion of sodium taurocholate).

2 urolithocholate), and hepatocyte ballooning (taurocholate).

3 res with greater apparent affinity than does taurocholate.

4 ure to glycine and certain bile acids, e.g., taurocholate.

5 difficile spores in response to cholate and taurocholate.

6 id palmitate, and the bile acids cholate and taurocholate.

7 e and the apical-to-basolateral transport of taurocholate.

8 nalicular membrane by cAMP and the other, by taurocholate.

9 onfers functional transport of the bile salt taurocholate.

10 est natural substrates being isethionate and taurocholate.

11 vered from explants after exposure to sodium taurocholate.

12 Na+-dependent transcellular transport of [3H]taurocholate.

13 nin was administered prior to treatment with taurocholate.

14 induced TcpP dimerization in the presence of taurocholate.

15 saturable, exhibiting a Km of 63 microM for taurocholate.

16 tides to MTP was enhanced in the presence of taurocholate.

17 n bile duct, and 3) intravenous infusions of taurocholate.

18 red with their redox state in the absence of taurocholate.

19 of cerulein or pancreatic infusion of sodium taurocholate.

20 an abiotic degradation of biofilm matrix by taurocholate.

21 rowth factor, interleukin-6, and then sodium taurocholate.

22 n or by intraductal administration of sodium taurocholate.

23 ne of these was revealed to be the bile salt taurocholate.

24 rats by intraductal infusion of 3.5% sodium taurocholate.

25 nt mice were protected from these effects of taurocholate.

26 r network formation similar to the effect of taurocholate.

27 The intraduodenal infusion of taurocholate (24 hours) in biliary-diverted rats resulte

28 g(-1) x min(-1)) on biliary recovery of (3)H-taurocholate ((3)H-TC) and (3)H-cholate administered int

29 [(3)H]Taurocholate ([(3)H]TC) uptake decreased in WIF-B cells

30 Intraduodenal infusion of taurocholate (36 micromol/h. 100 g rat(-1)) decreased SA

31 cile Ger receptors have not been identified, taurocholate (a bile salt) and glycine (an amino acid) h

32 Taurocholate (a bile salt) and glycine (an amino acid) h

33 the other hand, C. difficile spores require taurocholate (a bile salt) and glycine (an amino acid) t

34 films to physiologic levels of the bile salt taurocholate, a host signal for the virulence gene induc

35 porters from intrahepatic sites with cAMP or taurocholate, a significant increase in the amount of AB

36 Taurocholate accelerated canalicular network formation a

37 re germination kinetics to determine whether taurocholate acts as a specific germinant that activates

38 us BS (50%-80%, P < 0.05) as well as that of taurocholate administered in choleretic or trace radiola

39 Perfusion of liver with wortmannin before taurocholate administration blocked accumulation of all

40 ey further investigated the mechanism of how taurocholate affects V. cholerae virulence determinants.

41 only able to induce virulence in response to taurocholate after exit from the biofilm.

42 lesterol, 22.9% egg yolk lecithin, and 68.6% taurocholate (all mole %) were vitrified at 2 min to 20

43 -dependent induction, some bile salts (e.g., taurocholate) also activated the expression of cmeABC by

44 Uptake of [3H]taurocholate, an endogenous substrate of oatp1, was comp

45 Finally, a taurocholate analog with an m-aminobenzenesulfonic acid

46 ent study, we tested a series of glycine and taurocholate analogs for the ability to induce or inhibi

47 Testing of taurocholate analogs revealed that the 12-hydroxyl group

48 arly, C. difficile spores are able to detect taurocholate analogs with shorter, but not longer, alkyl

49 marked increases in tauro-beta-muricholate, taurocholate and 12-hydroxyeicosatetraenoic acid (12-HET

50 trafficking in rat liver induced by cAMP or taurocholate and [(35)S]methionine metabolic labeling fo

51 ectrokinetic chromatography system of sodium taurocholate and chromatographic measurements using an i

52 ase also regulate ATP-dependent transport of taurocholate and dinitrophenyl-glutathione directly in c

53 ATP-dependent transport of taurocholate and dinitrophenyl-glutathione in isolated c

54 n canalicular membrane vesicles and enhanced taurocholate and dinitrophenyl-glutathione transport in

55 ested the presence of distinct receptors for taurocholate and glycine.

56 levels of taurine, lathosterol, bile acids (taurocholate and glycodeoxycholate), nicotinamide, and a

57 constant intraduodenal infusion of micellar taurocholate and lecithin.

58 Of significance, oatp1-mediated taurocholate and LTC4 uptake was cis-inhibited and trans

59 nd cycloserine-cefoxitin mannitol broth with taurocholate and lysozyme (CCMB-TAL) were compared for r

60 o the plasma membrane; however, transport of taurocholate and other bile acids was abolished.

61 ATP-dependent transport of taurocholate and PI 3-kinase activity were reduced in ca

62 ease in sensitivity to the bile salts sodium taurocholate and sodium deoxycholate and significantly i

63 greater for the more hydrophilic bile salts taurocholate and tauroursodeoxycholate, for EYPC/Ch vesi

64 SPGP cells had decreased uptake of taurocholate and vinblastine compared with LLC-PK1 cells

65 mic response was blocked on co-infusion with taurocholate and was absent for infusion of the conjugat

66 d from rat liver that had been perfused with taurocholate and wortmannin.

67 -arginine, the retrograde infusion of sodium taurocholate, and another novel model with concomitant a

68 er progenitor cells (LPCs) were treated with taurocholate, and key events in DR evolution were assess

69 the monoanionic bile acids glycocholate and taurocholate, and methotrexate.

70 clodextrin as the chiral selector and sodium taurocholate as the micelle-forming agent.

71 more extensively reduced in the presence of taurocholate, as compared with their redox state in the

72 with conjugated bile salts (glycocholate and taurocholate) being less active than unconjugated bile s

73 Taurocholate bound to VcDsbA with a KD of 40 +/- 2.5 muM

74 ctose agar (CCFA), CCFA with horse blood and taurocholate (CCFA-HT), and cycloserine-cefoxitin mannit

75 whether it is the recently described sodium-taurocholate co-transporter polypeptide (NTCP), encoded

76 imulates taurocholate (TC) uptake and sodium taurocholate co-transporting polypeptide (Ntcp) transloc

77 ncipal hepatic bile acid importer, the Na(+)/taurocholate co-transporting polypeptide (Ntcp, Slc10a1)

78 ed in enterohepatic recirculation, the Na(+)-taurocholate co-transporting polypeptide (NTCP; also kno

79 secretion by studying the in vivo effect of taurocholate, colchicine, and wortmannin on bile acid se

80 able cell and heat-resistant spore counts on taurocholate-containing media.

81 The Na+-taurocholate cotransport polypeptide (ntcp) is the prima

82 The BA transporter high-affinity Na+ /taurocholate cotransporter (NTCP) and the BA synthesizin

83 l functions of the cytoplasmic tail of Na(+)/taurocholate cotransporter (Ntcp) and to determine the b

84 Expression and function of the hepatic Na+/taurocholate cotransporter (ntcp) are down-regulated in

85 the hepatocyte basolateral sodium-dependent taurocholate cotransporter (Ntcp) to decrease by more th

86 the circulation by the liver-specific sodium/taurocholate cotransporter (SLC10A1).

87 In contrast, expressions of the Na(+) taurocholate cotransporter and multispecific organic ani

88 or basolateral bile acid transporters sodium taurocholate cotransporter protein and organic anion tra

89 ther hepatocyte membrane transporters (Na(+) taurocholate cotransporter, multispecific organic anion

90 nd required TUDC uptake by way of the Na(+) /taurocholate cotransporting peptide.

91 rus and hepatitis delta virus use the sodium/taurocholate cotransporting polypeptide (a bile acid tra

92 sion of the HBV entry receptor, human sodium-taurocholate cotransporting polypeptide (hNTCP), on maca

93 host factors like the receptor human sodium taurocholate cotransporting polypeptide (hNTCP).

94 in HuH7 cells stably transfected with sodium taurocholate cotransporting polypeptide (HuH-NTCP cells)

95 polypeptide (OATP) 1B1 and sodium-dependent taurocholate cotransporting polypeptide (NTCP) allelic v

96 interact with the HBV entry receptor sodium taurocholate cotransporting polypeptide (NTCP) and impai

97 ation of plasma membrane localization of Na+-taurocholate cotransporting polypeptide (NTCP) and multi

98 Identification of sodium taurocholate cotransporting polypeptide (NTCP) as an HBV

99 The discovery of sodium taurocholate cotransporting polypeptide (NTCP) as the he

100 derlying the upregulation of the hepatic Na+/taurocholate cotransporting polypeptide (ntcp) by prolac

101 Sodium taurocholate cotransporting polypeptide (Ntcp) from the

102 Although sodium taurocholate cotransporting polypeptide (NTCP) has recen

103 tracellular Na(+) and the presence of sodium-taurocholate cotransporting polypeptide (NTCP) indicate

104 The sodium taurocholate cotransporting polypeptide (Ntcp) is the ma

105 The Na(+) -taurocholate cotransporting polypeptide (NTCP) mediates

106 ssion, protein mass, and function of the Na+/taurocholate cotransporting polypeptide (Ntcp), common b

107 porters (bile salt export pump (BSEP), Na(+)/taurocholate cotransporting polypeptide (NTCP), OATP1, O

108 Moreover, the Na+-taurocholate cotransporting polypeptide (NTCP), responsi

109 ity in HepG2 cells reconstituted with sodium taurocholate cotransporting polypeptide (NTCP), the curr

110 larity on short term regulation of the Na(+)-taurocholate cotransporting polypeptide (Ntcp), the majo

111 SLC10A1 codes for the sodium-taurocholate cotransporting polypeptide (NTCP), which is

112 e L protein subsequently binds to the sodium taurocholate cotransporting polypeptide (NTCP, encoded b

113 of bile acids from portal circulation is Na+-taurocholate cotransporting polypeptide (NTCP, SLC10A1).

114 The Na(+) -taurocholate cotransporting polypeptide (NTCP/SLC10A1) i

115 curred through the human HBV receptor sodium taurocholate cotransporting polypeptide but could not be

116 The Na(+)-taurocholate cotransporting polypeptide SLC10A1 (NTCP) p

117 lls were stably transfected with human Na(+)-taurocholate cotransporting polypeptide, BSEP, MDR3, and

118 peptide (Oatp) 1a1, Oatp1a4, Oatp1b2, sodium taurocholate cotransporting polypeptide, multidrug resis

119 he liver basolateral membrane protein, Na(+)/taurocholate cotransporting polypeptide, with the 14-mer

120 m-dependent bile acid transporter and Na(+) -taurocholate cotransporting polypeptide, within the ente

121 he farsenoid X receptor and sodium-dependent taurocholate cotransporting polypeptide; new insights in

122 conjugated bile acids, mediated by the Na(+)-taurocholate cotransporting protein (NTCP).

123 ted alterations of the hepatocellular sodium-taurocholate-cotransporting polypeptide (ntcp).

124 primers for both the rat liver Na+-dependent taurocholate-cotransporting polypeptide and rat ileal ap

125 orters OATP1B2, OATP1A1, OATP1A4, and Na(+) -taurocholate-cotransporting polypeptide only in central

126 We determined the increment in taurocholate-dependent bile flow and biliary lipid secre

127 to HCO3- extrusion, we compared the rates of taurocholate-dependent HCO3- efflux from alkali-loaded n

128 rt the model bile acid transporter substrate taurocholate (despite the pronounced sensitivity of both

129 Tbeta also were able to mediate transport of taurocholate, digoxin, and prostaglandin E2 but not of e

130 Conjugated cholate and taurocholate directly and secondary to primary BA ratio

131 In mice given taurocholate, DNase I administration also reduced expres

132 -dependent PKA inhibitor did not prevent the taurocholate effect.

133 ownstream targets, Rap1 and MEK, blocked the taurocholate effect.

134 Here we show that cholate and taurocholate elicit more dramatic Ca(2+) signals and nec

135 ulfophthalein or probenecid was observed for taurocholate, estrone sulfate, and para-aminohippurate i

136 ut not each separately, were able to take up taurocholate, estrone sulfate, digoxin, and prostaglandi

137 The stoichiometry of GSH/taurocholate exchange was 1:1.

138 Cell-free media from taurocholate-exposed biofilms contains a larger amount o

139 antibiotics, including the primary bile acid taurocholate for germination, and carbon sources such as

140 Furthermore, taurocholate, glycine, and chenodeoxycholate seem to bin

141 occurring bile salts: cholate, glycocholate, taurocholate, glycochenodeoxycholate, and taurochenodeox

142 of bilirubin, sulfobromophthalein (BSP), and taurocholate, had any influence on 55Fe-heme uptake.

143 ptone no. 2, and the concentration of sodium taurocholate has been reduced from 0.1% to 0.05%.

144 sis in animal models, whereas the bile acid, taurocholate, has protective effects in animal models of

145 ganic anion uptake, we examined transport of taurocholate in a HeLa cell line stably transfected with

146 Everted membrane vesicles accumulated taurocholate in an energy-dependent manner, apparently c

147 This study describes a unique function of taurocholate in bile canalicular formation involving sig

148 hus, we performed transport studies with [3H]taurocholate in confluent polarized monolayers of normal

149 As with Oatp1, uptake of 10 microM [(3)H]taurocholate in Oatp2-expressing Xenopus laevis oocytes

150 ecules are significantly more effective than taurocholate in promoting the intestinal absorption of a

151 Sodium-dependent uptake of (3)H-taurocholate in renal brush border membrane vesicles was

152 presence of the bile salts glycocholate and taurocholate in the small intestine causes dimerization

153 hosphate enhanced ATP-dependent transport of taurocholate in these vesicles above control levels.

154 of cholangiocytes, we examined the effect of taurocholate (in the absence or presence of wortmannin o

155 Taurocholate increased protein content of ATP-dependent

156 Infusion of taurocholate increased the transcriptional activity of N

157 y acid (propionate) and major rat bile acid (taurocholate) indicating a fundamental position for GLUT

158 stimulated by intracellular 0.2 mM unlabeled taurocholate, indicating bidirectional transport.

159 ng of LDL was enhanced by preincubation with taurocholate, indicating that partial delipidation of ap

160 additional administration of cAMP but not by taurocholate, indicating two distinct intrahepatic pools

161 Taurocholate induced a time-dependent increase in LPC pr

162 Sodium cholate and taurocholate induced cytosolic Ca(2+) elevations in stel

163 Infusion of taurocholate induced formation of NETs in pancreatic tis

164 Taurocholate induced LPCs to release MCP-1, MIP1alpha, a

165 Although taurocholate induced similar changes in circular dichroi

166 Inhibition of NFAT with A-285222 blocked taurocholate-induced activation of NFAT in all organs.

167 groups of rats with either mild or moderate taurocholate-induced AP and sham controls.

168 Wortmannin blocked taurocholate-induced bile acid secretion.

169 deoxycholate, another bile salt, can inhibit taurocholate-induced C. difficile spore germination.

170 Colchicine prevented taurocholate-induced changes in all proteins studied, as

171 Neutrophil depletion prevented taurocholate-induced deposition of NETs in the pancreas.

172 A-285222 also reduced taurocholate-induced increases in levels of amylase, mye

173 ve compounds were extracted and screened for taurocholate-induced necrosis in mouse pancreatic acinar

174 oinflammatory genes in vivo in the course of taurocholate-induced necrotizing pancreatitis in rats an

175 derivatives have been prepared that inhibit taurocholate-induced spore germination.

176 feres with bile acid secretion in rat liver; taurocholate induces recruitment of ATP-dependent transp

177 A taurocholate influx assay and membrane biotinylation ana

178 , or sodium taurocholate (the bile-depleted, taurocholate-infused group).

179 In bile-depleted, taurocholate-infused rats, cholangiocyte proliferation a

180 In addition, the bile acid taurocholate inhibited the uptake of 3H-MPP+ in oocytes

181 Taurocholate inhibited VcDsbA reductase activity without

182 Infusion of Na-taurocholate into the pancreatic duct induced necrotizin

183 P was induced in C57BL/6 mice by infusion of taurocholate into the pancreatic duct or by intraperiton

184 er induction of AP by retrograde infusion of taurocholate into the pancreatic ducts of wild-type, NFA

185 C transporter trafficking induced by cAMP or taurocholate is a physiologic response to a temporal dem

186 epatocyte cholate transport, suggesting that taurocholate is also taken up by an alternative system n

187 This study suggests that taurocholate is involved in initiating functional LPC bi

188 alogs revealed that the 12-hydroxyl group of taurocholate is necessary, but not sufficient, to activa

189 In the absence of extracellular Cl- and taurocholate, isohydric reduction of superfusate HCO3- c

190 iency leads to impaired biliary excretion of taurocholate, lecithin, and water, while cholesterol tra

191 sulin and glycaemic pathways, and related to taurocholate levels in blood.

192 ated with hepatic fibrosis stage and biliary taurocholate levels.

193 associated with higher grades of steatosis (taurocholate), lobular (glycocholate) and portal inflamm

194 Efflux of [3H]taurocholate mediated by CBATP in the cotransfected COS

195 These results indicate that taurocholate-mediated changes involve a microtubular sys

196 nodeoxycholate is a competitive inhibitor of taurocholate-mediated germination and appears to interac

197 enodeoxycholate, another bile acid, inhibits taurocholate-mediated germination.

198 e-sensitive uptake of [(3)H]cholesterol from taurocholate micelles.

199 yte containing gamma-cyclodextrin and sodium taurocholate micelles.

200 [(3)H]sn-2-monoolein was examined by adding taurocholate-mixed sn-2-18:1 and albumin-bound sn-2-18:1

201 e sn-2-18:1 was incorporated into TG from AP taurocholate-mixed sn-2-18:1, whereas more phospholipid

202 The position of the taurocholate molecule, together with the molecular archi

203 ependent process that tightly companied with taurocholate movement.

204 port measured and its inability to transport taurocholate, MRP3, like MRP1 and cMOAT, is concluded to

205 ered sequential progression of binding where taurocholate must be recognized first before detection o

206 Na+/taurocholate (Na+/TC) cotransport in hepatocytes is medi

207 Bile salts such as sodium taurocholate (NaTC) are routinely used to induce the exc

208 ar GSH failed to cis-inhibit uptake of [(3)H]taurocholate or [(3)H]digoxin in Oatp2-expressing oocyte

209 rats by either retrograde infusion of sodium taurocholate or by direct trauma.

210 hours, followed by intraduodenal infusion of taurocholate or fluid/electrolytes.

211 strain but only marginally more sensitive to taurocholate or glycocholate.

212 as enhanced in the presence of extracellular taurocholate or sulfobromophthalein, indicating that GSH

213 Infusion of either taurocholate or taurochenodeoxycholate for 12 hours also

214 ancreatic AR42J acinar cells stimulated with taurocholate or TNF-alpha.

215 fected by brefeldin A, dibutyryl cyclic AMP, taurocholate, or PI 3-kinase inhibitors.

216 ispersed in mixed micelles containing sodium taurocholate, phosphatidylcholine, and cholesterol remai

217 ling endosome, whereas recruitment from the "taurocholate-pool" involves a hepatocyte-specific mechan

218 M) structure was captured with the substrate taurocholate present, bound between the core and panel d

219 Taurocholate rapidly activated MEK, LKB1, and AMPK, whic

220 icroscopy micrographs of biofilms exposed to taurocholate revealed an altered, perhaps degraded, appe

221 en shown that a set of bile salts, including taurocholate, serve as host signals to activate V. chole

222 of rats or perfusion of isolated liver with taurocholate significantly increased PI 3-kinase activit

223 The inhibitory effect of wortmannin on taurocholate stimulation of cholangiocyte proliferation

224 In vitro, taurocholate stimulation of DNA replication and secretin

225 ncrease in bile flow and bile salt output in taurocholate-supplemented isolated perfused livers but h

226 ple and mixed micelles prepared using sodium taurocholate (TA) alone or with egg lecithin (LE) were t

227 cted cells showed virtually no uptake of [3H]taurocholate, taurocholate uptake by induced cells was N

228 Taurocholate (TC) and dibutyryl cAMP (DBcAMP), stimulato

229 Hepatic uptake of intravenously infused taurocholate (TC) and taurochenodeoxycholate (TCDC) were

230 Other natural bile acids, taurocholate (TC) and taurodeoxycholate (TDC), were also

231 By this mechanism, taurocholate (TC) and taurolithocholate (TLC) increase c

232 We have shown that taurocholate (TC) and taurolithocholate (TLC) interact i

233 nt bile flow and biliary lipid secretion and taurocholate (TC) biliary transit time during high ASBT

234 yclic AMP has been proposed to stimulate Na+/taurocholate (TC) cotransport in hepatocytes by transloc

235 P-mediated translocation of sinusoidal Na(+)/taurocholate (TC) cotransporter (Ntcp) to the plasma mem

236 ate (cAMP) stimulates translocation of Na(+)-taurocholate (TC) cotransporting polypeptide (Ntcp) and

237 tic bile acid uptake by translocating sodium-taurocholate (TC) cotransporting polypeptide (Ntcp) from

238 activity of the bile salt transporters Na(+)/taurocholate (TC) cotransporting polypeptide (Ntcp), and

239 d transport (ASBT)-mediated uptake of [(14)C]taurocholate (TC) in H14 cells.

240 by examining sodium-dependent uptake of [3H]-taurocholate (TC) into brush border membrane vesicles (B

241 We examined the effect of taurocholate (TC) on the basolateral uptake of [3H]TC in

242 Support livers were provoked with taurocholate (TC) to enhance bile aqueous and hydrophobi

243 ed by Northern blotting, immunoblotting, and taurocholate (TC) transport studies in isolated short-te

244 cell swelling- and cAMP-induced increases in taurocholate (TC) uptake and Ntcp translocation in hepat

245 Cyclic AMP stimulates taurocholate (TC) uptake and sodium taurocholate co-tran

246 bile acid clearance increased from 15% when taurocholate (TC) was infused alone to 46% (P=0.007) whe

247 ells, taurochenodeoxycholate (TCDC), but not taurocholate (TC), induced endocytosis of Ntcp.

248 d the effects of three different bile salts, taurocholate (TC), tauroursodeoxycholate (TUDC), and tau

249 ic acid (OA) together with either albumin or taurocholate (TC).

250 ne triphosphate (ATP)-dependent transport of taurocholate (TC, 1 micromol/L) in membrane vesicles was

251 er RNA (mRNA) levels in the following order: taurocholate (TCA) (288 +/- 36%, P <.005) = taurodeoxych

252 In primary rat hepatocyte cultures, taurocholate (TCA) strongly activated JNK in a time- and

253 e receptor 2 (S1P(2) ) as being activated by taurocholate (TCA).

254 P after administration of cerulein or sodium taurocholate than control mice; pancreata from the Bcl3(

255 nseleit (the bile-depleted group), or sodium taurocholate (the bile-depleted, taurocholate-infused gr

256 When wortmannin was given after taurocholate, the protein levels of each ATP-dependent t

257 en insulin was co-infused with the bile salt taurocholate, this was followed by a marked hypoglycaemi

258 ficile spores recognize both amino acids and taurocholate through multiple interactions that are requ

259 s show that when IBAT mediates uptake of [3H]taurocholate to a level 20-fold higher than that achieve

260 Administration of cAMP or taurocholate to rats increased amounts of SPGP, MDR1, an

261 Addition of 25 microM taurocholate to the superfusate led to a rapid fall in p

262 little skate Raja erinacea, was screened for taurocholate transport activity by using Xenopus laevis

263 of the C terminus of Ostbeta abolished [(3)H]taurocholate transport activity, but reinsertion of two

264 7G, G982R, R1153C, and R1268Q also abolished taurocholate transport activity, possibly by causing Bse

265 plasma membrane and generate cellular [(3)H]taurocholate transport activity.

266 Taurocholate transport by two assessed mutants, N490D an

267 lonal antibody that partially protects (26%) taurocholate transport from inhibition by DIDS in hepato

268 assessed by adenosine triphosphate-dependent taurocholate transport in canalicular membrane vesicles,

269 These data indicate that oatp-mediated taurocholate transport is Na+-independent, saturable, an

270 were identified as functionally important by taurocholate transport studies, substrate inhibition ass

271 cept is provided by the observation that the taurocholate transport system is almost completely prote

272 talpha(-/-) vs. wild-type mice; the residual taurocholate transport was further reduced to near-backg

273 Taurocholate transport was measured in isolated ileal br

274 Apical phospholipid secretion and taurocholate transport were assayed to investigate the e

275 ted from HTC-R cells exhibited ATP-dependent taurocholate transport, which was many-fold greater than

276 ate-stimulated ATPase activity as well as on taurocholate transport.

277 r plasma membrane fractions from control and taurocholate-treated rats correlated positively with ade

278 e mutated Ntcps followed by determination of taurocholate uptake and Ntcp expression, translocation a

279 ylation and in 2.5 and 3.2-fold increases in taurocholate uptake and Ntcp retention in the plasma mem

280 in postligation time accompanied by reduced taurocholate uptake by basolateral membrane.

281 wed virtually no uptake of [3H]taurocholate, taurocholate uptake by induced cells was Na+-independent

282 Interestingly, [(3)H]taurocholate uptake in Oatp2-expressing oocytes was also

283 [14C]-Taurocholate uptake into cholangiocytes was determined.

284 ere assessed by a combination of functional (taurocholate uptake into crude brush border membrane ves

285 Similarly, sodium-dependent [3H]taurocholate uptake into membrane vesicles from cholic a

286 bile duct ligation (CBDL) from 1-7 days, and taurocholate uptake was determined in isolated hepatocyt

287 Cells were analyzed for Myrcludex B binding, taurocholate uptake, HBV covalently closed circular DNA

288 in rapid down-regulation of Na(+)-dependent taurocholate uptake, ntcp transcription, and posttranscr

289 ll alanine insertions in H3 and H8 abolished taurocholate uptake, suggesting that both these regions

290 e mutants displayed active, sodium-dependent taurocholate uptake.

291 ontrast, the A171S mutation had no effect on taurocholate uptake.

292 Taurocholate, used to solubilize the fatty acids, did no

293 transfected with IBAT and CEA, efflux of [3H]taurocholate was detected in COS cells cotransfected wit

294 lein, and 2.6 mm [(14)C]cholesterol in 19 mm taurocholate was infused into the duodenum of lymph fist

295 A Na+-dependent saturable uptake of taurocholate was present in large but not small cholangi

296 gut sac experiments, transileal transport of taurocholate was reduced by >80% in Ostalpha(-/-) vs. wi

297 Sodium-dependent transport of taurocholate was shown to be dependent on the expression

298 lower than in WT mice, but the clearance of taurocholate was similar in the two genotypes.

299 Water-soluble vitamins B2, B5, B6, and taurocholate were also detected with high fold change in

300 closed saturable Na+-dependent uptake of [3H]taurocholate, with apparent Km and Vmax values of 209+/-