ライフサイエンスコーパス: tegument

1 p2) are expressed in the Schistosoma mansoni tegument.

2 ovel drugs targeting TEMs in the schistosome tegument.

3 connecting the inner tegument with the outer tegument.

4 the activated form of ERK2 (pERK2) into the tegument.

5 ICP0 results in the absence of ICP0 from the tegument.

6 have the potential to reach all parts of the tegument.

7 rtant for the structural organization of the tegument.

8 d contribute to the deformable nature of the tegument.

9 oteins of the herpes simplex virus 1 (HSV-1) tegument.

10 ion of Ov-TSP-2 and TSP-3 to the adult fluke tegument.

11 t-parasite interface, a structure called the tegument.

12 model of the protein organization inside the tegument.

13 to cytoplasmic membranes and into the virion tegument.

14 esent in mature virions as components of the tegument.

15 The major tegument 150-kDa phosphoprotein (pp150) of HCMV binds to

16 eins, most of which are contained within the tegument, a complex structural layer between the nucleoc

17 hin a proteinaceous capsid surrounded by the tegument, a layer of viral and cellular proteins.

18 the oral sensory papillae, acetabular ducts, tegument, acetabular glands, and nervous system.

19 sights into the poorly understood process of tegument acquisition.

20 importance of pUL36 in the initial stages of tegument addition and provides new insights into the pro

21 nses specific to soluble worm antigen (SWA), tegument allergen-like 1, and 28-kDa glutathione-S-trans

22 dral nucleocapsid surrounded by an amorphous tegument and a lipoprotein envelope.

23 ts icosahedral symmetry, but the surrounding tegument and envelope layers lack regular architecture.

24 An analysis of 11 tegument and envelope proteins defined the composition a

25 ly investigated, the precise organization of tegument and envelope proteins remains elusive.

26 ORF9 localized to the virion tegument and formed complexes with glycoprotein E, which

27 had frequent responses to glycoproteins and tegument and immediate-early (IE) proteins of HSV-2, T c

28 To clarify the structure of the tegument and its attachment to the capsid, we used elect

29 fected ECs inefficiently acquired the virion tegument and secondary envelope.

30 of connecting capsid and membrane across the tegument and that the ability to switch between monomeri

31 urring via numerous interactions between the tegument and the capsid, within the tegument, and betwee

32 capsid, within the tegument, and between the tegument and the envelope.

33 a process that is normally overcome by viral tegument and/or immediate-early proteins.

34 ween the tegument and the capsid, within the tegument, and between the tegument and the envelope.

35 ich were previously localized to the capsid, tegument, and envelope layers using traditional biochemi

36 structure consisting of a DNA-filled capsid, tegument, and envelope.

37 in 13/14 (VP13/14), also known as UL47, is a tegument antigen targeted by CD8(+) T cells from HSV-ser

38 to extend across the entire thickness of the tegument (approximately 50 nm).

39 y could serve as organizing features for the tegument, as they have the potential to reach all parts

40 our observations suggest that while complete tegument assembly may not be necessary for VZV replicati

41 The EBV tegument BNRF1 is a DAXX-interacting protein required fo

42 ccurs in the cytoplasm, where the capsid and tegument bud into host cell membranes.

43 SmAQP) that is found largely in the parasite tegument by immunolocalization and is most highly expres

44 is hypothesized to facilitate the budding of tegumented capsid into glycoprotein-embedded membrane du

45 protein, pUL36, which occupies the layer of tegument closest to the capsid, is essential for formati

46 cterization of a multicomponent glycoprotein-tegument complex found in herpes simplex virus 1 (HSV-1)

47 pp71 is a tegument component delivered directly to cells by infect

48 The alphaherpesvirus UL51 protein is a tegument component that interacts with the viral glycopr

49 face of the capsid to VP13/14 and associated tegument components.

50 The tegument comprises at least 20 proteins destined for del

51 The tegument consists of multiple, virus-encoded protein spe

52 nvelope layer, which encloses the capsid and tegument, contains viral transmembrane proteins anchored

53 respectively) in the KSHV capsid-associated tegument cryo-EM structure.

54 us cell fusion experiments demonstrated that tegument-delivered pp71 found in the cytoplasm of undiff

55 V infects incompletely differentiated cells, tegument-delivered pp71 remains in the cytoplasm, allowi

56 y in CD34(+) cells infected in vitro because tegument-delivered pp71 remains in the cytoplasm.

57 ifferentiated cells block the trafficking of tegument-delivered pp71 to the nucleus or whether differ

58 fferentiated fibroblasts infected with HCMV, tegument-delivered pp71 traffics to the nucleus and degr

59 ed cells facilitate the nuclear transport of tegument-delivered pp71.

60 Lytic infection is not initiated because the tegument-delivered transactivator protein pp71 fails to

61 e organized in a gammaherpesvirus, with five tegument densities capping each penton vertex, a pattern

62 Each KSHV tegument density can be divided into three prominent reg

63 As the tegument does not have a regular structure, the question

64 Viral tegument, envelope, and some nonstructural proteins loca

65 The disruption in tegument formation from ORF52 suppression, therefore, pr

66 xport by budding into the perinuclear space, tegument formation, and envelopment to complete de novo

67 (DNA polymerase auxiliary subunit) and UL46 (tegument) had no measurable influence, while two indepen

68 ts, the icosahedral capsid and the amorphous tegument, has been extensively studied, but the identity

69 specially with respect to acquisition of the tegument; however, it is thought to involve the stepwise

70 The function of tegument ICP0 is unknown.

71 some the blocked entry of virions containing tegument ICP0, including ICP0 mutants that are defective

72 s domain contained greatly reduced levels of tegument ICP0, suggesting that the domain influences the

73 etofore unrecognized role of the schistosome tegument in controlling water and drug movement into the

74 arasites and highlight the importance of the tegument in parasite osmoregulation and drug uptake.

75 virus was treated in the same way; (ii) the tegument in TX-100-treated extracellular virions was asy

76 ut the protein is also packaged in the viral tegument, indicating that BPLF1 may function both early

77 36 stabilizes other components of the vertex-tegument interface.

78 f assembly, during which the majority of the tegument is acquired and final envelopment occurs.

79 The herpes simplex virus 1 (HSV-1) tegument is known to contact the capsid at its vertices,

80 irus, the interaction between the capsid and tegument is limited to the capsid vertices and involves

81 Assembly of the herpesvirus tegument is poorly understood but is believed to involve

82 undergo a time-dependent change in which the tegument is transformed from a state in which it is symm

83 nal is sent from the virion surface into the tegument is unknown.

84 hich the US3 kinase is incorporated into the tegument layer (between the capsid and envelope) in HSV

85 d is, in turn, surrounded by a proteinaceous tegument layer and a lipid envelope.

86 DNA-containing nucleocapsid surrounded by a tegument layer and host-derived lipid envelope studded w

87 ent protein were of smaller size because the tegument layer between capsid and viral envelope was red

88 ICP0 that is present in the tegument layer has not been well characterized.

89 The tegument layer of herpesviruses comprises a collection o

90 ntry, since ICP0 is a component of the inner tegument layer of the virion.

91 that the ORF7 protein is a component of the tegument layer of VZV virions.

92 Herpesviral virions contain a tegument layer that consists primarily of viral proteins

93 ant enveloped particles with thicker, oblong tegument layers.

94 sviruses, contain a protein layer termed the tegument localized between the capsid and the envelope.

95 It is well known that proteins in the tegument (located between the viral capsid and envelope

96 resides in the innermost layer of the viral tegument, lying between the capsid and the envelope.

97 aptive evolutions in homeostasis regulation, tegument maintenance and lipid uptakes, and differential

98 n fluorescent protein (GFP) and contains the tegument multifunctional ORF45 protein as a fusion prote

99 tracellular particles, having neither a full tegument nor an envelope.

100 Confocal studies on sections of tegument, nucellus, endosperm, and embryo showed that th

101 The tegument of all herpesviruses contains a capsid-bound la

102 The tegument of all herpesviruses contains a high-molecular-

103 UL21 is a conserved protein in the tegument of alphaherpesviruses and has multiple importan

104 croscopy and protein analysis to examine the tegument of herpes simplex virus type 1 (HSV-1).

105 The tegument of herpesviruses is a highly complex structural

106 und prominently in the dorsal surface of the tegument of males.

107 sid proteins, suggesting its presence in the tegument of the HSV-1 virion.

108 found that VZV ORF12 protein, located in the tegument of virions, enhances AP-1 reporter activity.

109 k in maturation limbo, unable to acquire the tegument or outer (envelope) layers.

110 The tegument, or middle layer, of herpesviruses comprises bo

111 It was observed that while the amount of tegument present in virions was not affected by time of

112 Epstein-Barr virus (EBV) BPLF1 encodes a tegument protein (3,149 amino acids) that exhibits deubi

113 cytomegalovirus (HCMV) high-molecular-weight tegument protein (HMWP, pUL48; 253 kDa) and the HMWP-bin

114 We identified most capsid proteins, a tegument protein (VP22), a glycoprotein (gD), and a cell

115 There results indicate that ORF52 is a tegument protein abundantly present in extracellular vir

116 (KSHV) is an immediate-early phosphorylated tegument protein and has been shown to play important ro

117 , we show that the viral protein 1/2 (VP1/2) tegument protein associates with the dynein/dynactin mic

118 In summary, the EBV tegument protein BGLF2, which is delivered to the cell a

119 Here, we demonstrate that the EBV tegument protein BNRF1 binds the histone H3.3 chaperone

120 We show that the EBV tegument protein BNRF1 functions to regulate chromatin a

121 We propose that EBV tegument protein BNRF1 replaces ATRX to reprogram Daxx-m

122 We report that EBV tegument protein BNRF1, discovered by other investigator

123 A herpes simplex virus tegument protein brought into the cell during infection

124 We find that HCMV virion binding and tegument protein delivery are insufficient for HCMV-medi

125 For example, the pp71 tegument protein encoded by the UL82 gene of human cytom

126 PTAP motif within pp150/ppUL32, an essential tegument protein involved in the last steps of viral mat

127 xpression and localization of MDV pUL47-EGFP tegument protein is potentially important for the unique

128 s of both the major capsid protein (M86) and tegument protein M25 were reduced in the absence of the

129 on and extends our understanding of the HCMV tegument protein network that is required to interface t

130 The UL16 tegument protein of herpes simplex virus (HSV) is conser

131 UL16 is a tegument protein of herpes simplex virus (HSV) that is c

132 The UL16 tegument protein of herpes simplex virus 1 (HSV-1) is co

133 The UL11 tegument protein of herpes simplex virus plays a critica

134 sly shown that ORF45, an immediate-early and tegument protein of Kaposi's sarcoma-associated herpesvi

135 The ORF75c tegument protein of murine gammaherpesvirus 68 (MHV68) p

136 a KSHV immediate-early protein as well as a tegument protein of virions, interacts with IRF-7 and in

137 se (DUB) activity, embedded within the large tegument protein open reading frame (ORF)64, gained the

138 , we show that the gammaherpesvirus-specific tegument protein ORF52 is critical for maturation of RRV

139 tease (USP) domain embedded within the large tegument protein ORF64, as do all other herpesviruses.

140 The HCMV virion tegument protein pp150 (ppUL32) is an essential protein

141 ) of infected cells co-localizing with virus tegument protein pp150 and the formation of vAC was comp

142 P, Tri1, Tri2, and SCP and the HCMV-specific tegument protein pp150-totaling 4000 molecules and 62 d

143 seen in the absence of the immunomodulatory tegument protein pp65 (pUL83).

144 s (HCMV) immediate early protein IE1 and the tegument protein pp71 are required for efficient infecti

145 E protein IE1 targets PML and Sp100, and its tegument protein pp71 targets hDaxx and ATRX.

146 Tegument protein pp71 was cytoplasmic, and immediate-ear

147 start of lytic infection by the HCMV virion tegument protein pp71, which upon viral entry traffics t

148 d the conserved, multifunctional HCMV virion tegument protein pUL103 as important for cVAC biogenesis

149 We previously showed that the HCMV tegument protein pUL103 is required for cVAC biogenesis.

150 capsid protein pUL25 and the capsid-tethered tegument protein pUL36.

151 oteins, pUL17 and pUL25, and the large inner tegument protein pUL36.

152 cruited to HSV-1 capsids by the capsid-bound tegument protein pUL37 to promote efficient cytoplasmic

153 cent protein (EGFP) to the C terminus of the tegument protein pUL47 (vUL47-EGFP) or pUL49 (vUL49-EGFP

154 The HCMV tegument protein pUL83 inhibits this response by interac

155 The virion host shutoff (VHS) RNase tegument protein released into cells by infecting virus

156 rus (HCMV) UL99-encoded pp28 is an essential tegument protein required for envelopment and production

157 have now revealed this species to be UL21, a tegument protein that has been implicated in the transpo

158 gammaherpesvirus 68 (MHV68), which encodes a tegument protein that induces PML degradation.

159 ific mAb for VZV ORF9, a membrane-associated tegument protein that interacts with glycoprotein E (gE)

160 consistent with an enzymatic function of the tegument protein that is beneficial to the virus during

161 Human cytomegalovirus UL103 encodes a tegument protein that is conserved across herpesvirus su

162 We demonstrate here that UL94 is a tegument protein that is expressed with true-late kineti

163 herpesvirus (KSHV) is an immediate-early and tegument protein that plays critical roles in antagonizi

164 pp150 is a capsid-proximal tegument protein that preserves the integrity of nucleoc

165 ated herpesvirus, ORF52 is a highly abundant tegument protein tightly associated with the capsid.

166 elopment and that it interacts with the UL37 tegument protein to facilitate cytoplasmic virion envelo

167 The HSV-1-encoded tegument protein UL16 is involved in multiple events of

168 Inner tegument protein UL37 is conserved among all three subfa

169 detected by the colocalization of the virion tegument protein UL37, with dynein required for loading

170 s gD and gB and the novel T cell antigen and tegument protein UL40, and we compared this vaccine to a

171 We found that (i) HSV-1 tegument protein UL46 interacts with and colocalizes wit

172 ne of the least-studied proteins of HSV, the tegument protein UL46, and that function involves the ev

173 l proteins interacting with PABPC1 including tegument protein UL47 and infected-cell protein ICP27.

174 The human cytomegalovirus tegument protein UL69 has been shown to be required for

175 The human cytomegalovirus (HCMV) tegument protein UL69 is important for efficient viral r

176 rated that UL99 interacts with the essential tegument protein UL94 in infected cells as well as in th

177 ic assembly complex shows that the essential tegument protein UL99 (pp28) exhibits aberrant localizat

178 The HCMV tegument protein UL99 (pp28) is essential for viral repl

179 ionally, a deletion of the gene encoding the tegument protein Vhs ablated most of the depletion of AT

180 The herpes simplex virus (HSV) tegument protein VP1-2 contains an N-terminal nuclear lo

181 e of the herpes simplex virus type 1 (HSV-1) tegument protein VP1-2 originated from the analysis of t

182 enuated by antibodies specific for the viral tegument protein VP1/2 (UL36 gene) but not by similar an

183 fic to three epitopes derived from the HSV-1 tegument protein VP13/14 (VP13/14286-294,VP13/14504-512,

184 nity and UL19 (capsid protein VP5) and UL47 (tegument protein VP13/14) as T cell immunogens.

185 munogens UL19 (capsid protein VP5) and UL47 (tegument protein VP13/14) would enhance the protection p

186 ents and fluorescence detection of the HSV-1 tegument protein VP16 were used to analyze the mechanism

187 We demonstrate that the tegument protein VP22 bridges a complex between glycopro

188 However, levels of the tegument protein VP22 were also dramatically reduced in

189 while expression of the nonglycosylated IE62 tegument protein was unchanged.

190 Virions lacking the M25 tegument protein were of smaller size because the tegume

191 virus 8 replication and uptake of the ORF45 tegument protein were tested in human retinal pigment ep

192 These results indicate that a viral tegument protein which is delivered to cells upon infect

193 IG-I)-deficient cells and that KSHV ORF64, a tegument protein with deubiqutinase (DUB) activity, supp

194 virus 1 (HSV-1) U(L)21 gene encodes a 62-kDa tegument protein with homologs in the alpha-, beta-, and

195 to regulate not just the interaction of this tegument protein with its viral binding partners but als

196 s report, we further characterize ORF52 as a tegument protein with vital roles during KSHV lytic repl

197 ream of ORF70 (thymidylate synthase), ORF19 (tegument protein), and ORF47 (glycoprotein L) uncovered

198 UL23 (thymidine kinase), UL25 (DNA packaging tegument protein), and UL52 (helicase-primase primase su

199 t by similar antibodies specific for UL37 (a tegument protein), the major capsid protein (VP5), or VP

200 H), ORF25 (major capsid protein) and ORF64 (tegument protein).

201 e most frequently recognized L Ag, the BNRF1 tegument protein, also recognized latently infected, gro

202 e 52 (ORF52), a multifunctional and abundant tegument protein, as being the only virally encoded comp

203 We identified a viral tegument protein, BGLF2, which activates members of the

204 As might be expected for a tegument protein, interactions were identified that sugg

205 Through its interactions with pUL47, another tegument protein, it spares from degradation viral mRNAs

206 g trafficking to the AC of another essential tegument protein, pp28, or the viral glycoprotein comple

207 ons in the genes for pUL36 and another inner tegument protein, pUL37, to analyze the contributions of

208 lex in infected cells with another conserved tegument protein, pUL7.

209 ORF52, an abundant gammaherpesvirus-specific tegument protein, subverts cytosolic DNA sensing by dire

210 ucture of the N-terminal half of a conserved tegument protein, UL37, from HSV-1.

211 re--the first for any alphaherpesvirus inner tegument protein--reveals an elongated molecule of a com

212 During primary infection, tegument protein-specific B cells expressed an activated

213 h and similar frequencies of gB-specific and tegument protein-specific B cells following primary HCMV

214 equency and the phenotype of gB-specific and tegument protein-specific B cells were studied in a coho

215 hat a late 3.1 kb mRNA encodes a 130 kDa M25 tegument protein.

216 ding partner of the membrane-associated UL11 tegument protein.

217 ivity of the viral virion host shutoff (vhs) tegument protein.

218 ely due to its roles in virion assembly as a tegument protein.

219 a gammaherpesvirus-specific immediate-early tegument protein.

220 ore, genes encoding latent, early lytic, and tegument proteins and glycoproteins were found to contai

221 teins, including 13 novel interactions among tegument proteins and one novel interaction between caps

222 lieved to involve interactions between outer tegument proteins and the cytoplasmic domains of envelop

223 prehensive investigation of the functions of tegument proteins and their roles in viral replication m

224 protein interactions involving virus-encoded tegument proteins are critical for virus assembly and ar

225 However, several tegument proteins are known to be essential for proper p

226 s, for the first time, how capsid-associated tegument proteins are organized in a gammaherpesvirus, w

227 As no other tegument proteins are retained in significant amounts, i

228 ed to bind directly to the capsid with other tegument proteins bound indirectly by way of UL36.

229 Certain tegument proteins can also regulate viral processes.

230 Simultaneous tracking of capsids and tegument proteins demonstrated that the composition of a

231 a hub protein and play a role in recruiting tegument proteins during tegumentation and virion assemb

232 , little is known about the function of many tegument proteins during virus replication.

233 The tegument proteins encoded by ORF11 and ORF9 of varicella

234 d but lacking a membrane and depleted of all tegument proteins except UL36 and a second high-molecula

235 Whether and how such tegument proteins exist in gammaherpesviruses have been

236 Here we focus on two tegument proteins from herpes simplex virus 1 (HSV-1), p

237 Our study focuses on two tegument proteins from herpes simplex virus 1 that are c

238 However, no such tegument proteins have been identified for gammaherpesvi

239 Capsid-associated tegument proteins have been identified in alpha- and bet

240 Several capsid-associated tegument proteins have been identified in the alpha- and

241 Tegument proteins have important roles in assembling vir

242 Systematic exploration of the roles of tegument proteins in capsid trafficking requires detaile

243 the U(L)49 gene, is one of the most abundant tegument proteins in HSV-1 virions.

244 mensional structure of the capsid-associated tegument proteins in the prototypical member of gammaher

245 ORF64 was found to interact with several tegument proteins including ORF11, ORF21, ORF33, ORF45,

246 The incorporation of tegument proteins into the herpes simplex virus 1 (HSV-1

247 support the notion that the incorporation of tegument proteins into the herpesviruses is a very compl

248 eins required for cellular entry, as well as tegument proteins involved in regulating lytic replicati

249 ral mutants, which showed that each of these tegument proteins is critical for processing, transport,

250 t the organization of KSHV capsid-associated tegument proteins is highly similar to that in alphaherp

251 gG responses were frequently detected to the tegument proteins KSHV ORF38 and EBV BBRF and BGLF2 and

252 first time that these conserved herpesvirus tegument proteins localize to focal adhesions in additio

253 The tegument proteins of herpesviruses, including Kaposi's s

254 UL46 is one of the most abundant tegument proteins of HSV-1, but a well-established funct

255 Transient expression of the viral tegument proteins of pp71 and UL26 reduced NICD1 and Jag

256 may provide flexibility in interacting with tegument proteins or the DNA-packaging machinery at the

257 posi's sarcoma-associated herpesvirus (KSHV) tegument proteins ORF33 and ORF45 is crucial for progeny

258 Tegument proteins play both structural and regulatory fu

259 These tegument proteins play essential roles in viral propagat

260 Viral tegument proteins play important roles in maintaining th

261 Tegument proteins pp150 and pUL96 function at a late ste

262 intense investigation, the function of many tegument proteins remains unknown.

263 ail, virion packaging of UL11, but not other tegument proteins such as VP22 and VP16, was reduced by

264 mechanism of reactivation in the absence of tegument proteins that enable gene expression in product

265 systematically evaluated the ability of KSHV tegument proteins to modulate the activation of an integ

266 s function of gE requires the cooperation of tegument proteins UL11, UL16, and UL21.

267 Tegument proteins UL16 and UL11 of herpes simplex virus

268 The tegument proteins UL36 and UL37 are known to be transpor

269 the direct interaction between two essential tegument proteins VP1/2 and VP16 is required for connect

270 major HSV-1 capsid protein VP5; and the HSV tegument proteins VP11/12 (pU(L)46) and VP13/14 (pU(L)47

271 d analysis of the radial distribution of the tegument proteins VP16, VP1/2 and pUL37, and envelope pr

272 In addition, several tegument proteins were found in high-molecular-weight co

273 ruses (alpha, beta, and gamma) encode unique tegument proteins with specialized functions.

274 mpared with production of antibodies against tegument proteins, and this likely reduces the control o

275 iminate envelope-anchored glycoproteins from tegument proteins, both in purified virions and in virio

276 providing a flexible scaffold to which other tegument proteins, including UL37, bind.

277 new protein-protein interactions among HCMV tegument proteins, some of which are likely important fo

278 ding genes encoding latent, early lytic, and tegument proteins, such as substitutions within transmem

279 the incorporation of ORF45, as well as other tegument proteins, suggesting that ORF52 is important fo

280 Using 33 known capsid and tegument proteins, we tested 1,089 pairwise combinations

281 implex virus 1 UL11 and cytomegalovirus UL99 tegument proteins, which are involved in the final envel

282 e viruses is controlled by capsid-associated tegument proteins, yet their specific roles have not yet

283 y shrouding the pentons that we attribute to tegument proteins.

284 carboxyl termini of viral glycoproteins and tegument proteins.

285 iated with capsids, features common to inner tegument proteins.

286 rpesviruses, two capsid-associated, or inner tegument, proteins, UL37 and UL36, control cytosolic tra

287 -TSP-3) that are abundantly expressed in the tegument proteome of O. viverrini.

288 he UL16-gE interaction may play roles in the tegument signaling mechanism, virus budding, and the gE-

289 ern of immunogold-labeled SmNPP-5 within the tegument; some immunogold particles are scattered throug

290 Despite the high level of tegument structural similarities between KSHV and alphah

291 tudies confirm the importance of CD63 family tegument tetraspanins in parasitic flukes and support ef

292 esviruses have a layer of protein called the tegument that lies between the virion membrane and the c

293 nstrated that, compared to capsids lacking a tegument, these capsids (called T36 capsids) had tufts o

294 flukes resulted in phenotypes with increased tegument thickness, increased vacuolation (tsp-2) and re

295 stone deacetylases (HDACs) because pp71, the tegument transactivator that travels to the nucleus and

296 The HSV type 1 tegument virion phosphoprotein (VP) 11/12 (VP11/12) is a

297 of intact virions revealed that whereas the tegument was asymmetrically distributed around the capsi

298 strated that: (i) in extracellular virus the tegument was resistant to removal with Triton X-100 (TX-

299 unextracted virus; and (iii) in some images, tegument was seen to be linked to the capsid by short, r

300 e CDK1 and cyclin B1 were present in the VZV tegument with IE62 and were sensitive to detergent treat

301 nd VP16 is required for connecting the inner tegument with the outer tegument.