ライフサイエンスコーパス: temperature-sensitive mutant

1 he cytoplasm by inactivation of Kap121p in a temperature-sensitive mutant.

2 M17, suppress the growth defect of a tim54-1 temperature-sensitive mutant.

3 r arrest, elutriation, and arrest of a cdc15 temperature-sensitive mutant.

4 of ERK2 for aggregation by creating an ERK2 temperature-sensitive mutant.

5 Schizosaccharomyces pombe U2AF(59) (prp2.1) temperature-sensitive mutant.

6 is sufficient to support anaphase in an esp1 temperature-sensitive mutant.

7 Sts1 suppression, we characterized sts1-2, a temperature-sensitive mutant.

8 utations that enhance the lethality of a ftz temperature-sensitive mutant.

9 ression profiles in the wild-type and abf1-1 temperature-sensitive mutant.

10 he cell-cycle-arrested phenotype of an HCF-1 temperature-sensitive mutant.

11 th of these promoters was also seen with the temperature-sensitive mutants.

12 hane sulfonate (MMS)-sensitive mutants and 5 temperature-sensitive mutants.

13 from an independently derived collection of temperature-sensitive mutants.

14 P and accumulate larger forms in the RNase P temperature-sensitive mutants.

15 lusion effects, we examined Z rings in dnaA (temperature sensitive) mutants.

16 The structure of the adenovirus type 2 temperature-sensitive mutant 1 (Ad2ts1) was determined t

17 e cells (A1-5) that constitutively express a temperature-sensitive mutant (135 AV) of mouse p53 also

18 Experiments with the p53 temperature sensitive mutant 143ala revealed that at 32

19 Temperature-sensitive mutant 2-20/32 of Mycobacterium sm

20 In one such temperature-sensitive mutant, a highly conserved asparag

21 ost following conformational shifting of the temperature-sensitive mutant A135V.

22 se (ACC1FAS3) in Saccharomyces cerevisiae, a temperature-sensitive mutant (acc1(ts)) was constructed.

23 second line of transgenic mice containing a temperature-sensitive mutant allele of p53 also exhibits

24 erformed in strains expressing a conditional temperature-sensitive mutant allele of the hsp82 gene, w

25 tinct from those of other yeast enzymes, and temperature-sensitive mutant alleles abolish enzymatic a

26 The temperature-sensitive mutant alp5-1134 contains a single

27 stress has been traditionally studied using temperature-sensitive mutants and chemical inhibitors.

28 export specificity, it was necessary to use temperature-sensitive mutants and establish whether flag

29 The temperature-sensitive mutants arrest as viable, large, u

30 uct is localized to the nucleus, and an oaf1 temperature-sensitive mutant arrests as large budded cel

31 ees C with those in the widely used pat1-114 temperature-sensitive mutant at 34 degrees C, a temperat

32 We have isolated a temperature-sensitive mutant (bir1-107) in the essential

33 3 mutants and after a temperature shift of a temperature-sensitive mutant, cdc12, defective in the ne

34 s to complement fission yeast profilin SpPRF temperature-sensitive mutant cdc3-124 cells.

35 We now describe a novel temperature-sensitive mutant, cdc42-6, that reveals a ro

36 Using the ts85 temperature-sensitive mutant cell line, which expresses

37 and poly(A)+ RNA in the tsBN2 cells, a RCC1 temperature-sensitive mutant cell line.

38 Indeed, in pkh-ts temperature-sensitive mutant cells and in cells expressi

39 shift to the restrictive temperature, cdc20 temperature-sensitive mutant cells block at the onset of

40 nt of Cdc6 fails to complement either cdc6-1 temperature-sensitive mutant cells or Deltacdc6 null mut

41 ormant cells and the complementation of cdc6 temperature-sensitive mutant cells.

42 opy expression of msc1 robustly suppresses a temperature-sensitive mutant (cnp1-1) in the centromere-

43 We searched a temperature-sensitive mutant collection for strains with

44 n analysis of mitochondrial RNAs in an atp25 temperature-sensitive mutant confirmed that Atp25p is re

45 The wbbL of the temperature-sensitive mutant contained a single-base cha

46 ion by the ClpP protease, because in a groES temperature-sensitive mutant, CRAG was stable and accumu

47 Both a temperature-sensitive mutant, Cts56, and an isatin-beta-

48 tivity on the functional conformation of the temperature-sensitive mutant cystic fibrosis channel (F5

49 ssc1--2 is a temperature-sensitive mutant defective in both transloca

50 The chitin ring does not form normally in temperature-sensitive mutants defective in any of four s

51 e we present the identification of three new temperature-sensitive mutants defective in localization

52 We identified 20 temperature-sensitive mutants defective in pre-mRNA spli

53 ring nonpermissive infections performed with temperature-sensitive mutants defective in the F10 kinas

54 We designed a screen for temperature-sensitive mutants defective in the process o

55 One temperature-sensitive mutant demonstrated a striking pH

56 Genetic analysis of the outer core in a temperature-sensitive mutant demonstrated this core func

57 tructural proteins and from the thermolabile temperature-sensitive mutants described for T4 lysozyme.

58 mutant cdc2-D217N, we have isolated a novel temperature-sensitive mutant, dim1-35.

59 missive conditions in extracts of the cdc9-2 temperature-sensitive mutant, DNA ligation in both BER a

60 Of the four temperature-sensitive mutants evaluated in mice, one (A-

61 by inactivation of a kinesin, FLA10, in the temperature-sensitive mutant, fla10, existing flagella r

62 involved in karmellae assembly, we screened temperature-sensitive mutants for karmellae assembly def

63 Temperature-sensitive mutants for reg6 have profound def

64 The dnaN159 allele encodes a temperature-sensitive mutant form of the beta sliding cl

65 A temperature-sensitive mutant form of the yeast alpha-fac

66 Steinberg observed that strains containing a temperature-sensitive mutant form of tryptophanyl-tRNA s

67 In WC5 cells, which express a temperature-sensitive mutant form of v-Src, the complex

68 gated this interaction in cells expressing a temperature-sensitive mutant form of v-Src.

69 sequences of depleting CgtAC, we generated a temperature-sensitive mutant, G80E.

70 ings was heterogeneously affected in an ftsI temperature-sensitive mutant grown at the nonpermissive

71 r TFIID, disrupts cell growth control in the temperature-sensitive mutant hamster cell line ts13.

72 -PstI fragment complemented E. coli RO138, a temperature-sensitive mutant harboring lpxA2.

73 YaeT in E. coli was investigated with a new temperature-sensitive mutant harboring nine amino acid s

74 The loss of Srp1 is lethal, although several temperature-sensitive mutants have been described.

75 We isolated a temperature-sensitive mutant, hrd4-1, deficient in ER-as

76 A temperature-sensitive mutant in HRP1 yields mRNAs with s

77 A conditional lethal, temperature-sensitive mutant in one of the Aspergillus P

78 We took advantage of a temperature-sensitive mutant in PMA1, encoding the plasm

79 We have generated the first temperature-sensitive mutant in the Cvt pathway, designa

80 In addition, an HSV-1 temperature-sensitive mutant in the UL15 gene (ts66.4) i

81 Disruption of the head module in a temperature-sensitive mutant in vivo leads to the releas

82 Temperature-sensitive mutants in both pUL25 and pUL36 do

83 classical genetics in zebrafish to identify temperature-sensitive mutants in caudal fin regeneration

84 We show that rescue of temperature-sensitive mutants in parE and parC (encoding

85 Temperature-sensitive mutants in SFI1 show a defect in S

86 Analysis of temperature-sensitive mutants in the choline acetyltrans

87 luenzae lpxA and lpxB are able to complement temperature-sensitive mutants in the equivalent genes in

88 SEC35 was identified in a novel screen for temperature-sensitive mutants in the secretory pathway o

89 Spores carrying temperature-sensitive mutants in the spoVA operon were a

90 gene previously identified by analysis of a temperature-sensitive mutant, in the transient expressio

91 vestigated lipid synthesis in several Sec(-) temperature-sensitive mutants, including sec13-1.

92 An enp1-1 temperature-sensitive mutant inhibited 35S pre-rRNA earl

93 Surprisingly, the pan1-20 temperature-sensitive mutant is constitutively defective

94 Indeed, the anaphase block in rsi1/apc2 temperature-sensitive mutants is overcome by removal of

95 dopsis thaliana radially swollen 4 (rsw4), a temperature-sensitive mutant isolated previously on the

96 e amide dynamics studies were conducted on a temperature-sensitive mutant (L75F-TrpR) of the tryptoph

97 We have isolated a temperature-sensitive mutant (MB1) harboring the S22C an

98 post-docking events, we generated a Munc18c temperature-sensitive mutant (Munc18c/TS) by substitutio

99 urther test the role of Myo5, we generated a temperature-sensitive mutant myo5 allele.

100 A temperature-sensitive mutant named Lud135 was isolated w

101 Characterization of two temperature-sensitive mutants, nse5-ts1 and nse5-ts2, de

102 Two temperature-sensitive mutants, ntf2-1 and ntf2-2, that e

103 otent haemopoietic cell line FDCP-Mix with a temperature sensitive mutant of Bcr-Abl we have develope

104 Through the use of a conditional temperature sensitive mutant of Myb, we show that in the

105 Using a temperature sensitive mutant of the p53 (Trp53) gene, we

106 h of fibroblasts reversibly transformed by a temperature sensitive mutant of v-Src (ts LA 29).

107 l interaction, and they are able to suppress temperature sensitive mutants of one another when overex

108 Utilizing temperature sensitive mutants of tor2 and tap42, we exam

109 ility of RPM2 alleles to suppress tom40-3, a temperature-sensitive mutant of a component of the mitoc

110 ROS is not observed in cells infected with a temperature-sensitive mutant of Ad2, ts1, which is defec

111 Additionally, a temperature-sensitive mutant of Ad5 unable to penetrate

112 A temperature-sensitive mutant of AspB shows phenotypic ab

113 per (Spk) in apical branching of ramosa-1, a temperature-sensitive mutant of Aspergillus niger.

114 The defect in a temperature-sensitive mutant of Autographa californica n

115 Using cell lines transformed by a temperature-sensitive mutant of BCR/ABL, these kinetic a

116 aphase-promoting complex (APC), cdc23(ts); a temperature-sensitive mutant of cdc20; and a cdh1-null m

117 We describe a new temperature-sensitive mutant of Chinese hamster cell fib

118 ream region of highly transcribed genes in a temperature-sensitive mutant of Clp1 at elevated tempera

119 ed immunity to coccidioidomycosis, we used a temperature-sensitive mutant of Coccidioides immitis to

120 2 is essential for cell viability and that a temperature-sensitive mutant of dpb2 arrests with a 1C D

121 Here we use a temperature-sensitive mutant of dynamin (G273D) to contr

122 significantly altered in cells expressing a temperature-sensitive mutant of dynamin under conditions

123 by the use of ts20TGR cells, which contain a temperature-sensitive mutant of E1, the ubiquitin-activa

124 We have identified a novel temperature-sensitive mutant of fission yeast alpha-tubu

125 ated tRNA in cells; and 3) in cells having a temperature-sensitive mutant of histidyl tRNA synthetase

126 We used a unique temperature-sensitive mutant of Hsp90 in Saccharomyces c

127 tion was carried out between the genome of a temperature-sensitive mutant of MHV-A59 (Alb4) and RNA t

128 S) of FVB/N mice that are infected by ts1, a temperature-sensitive mutant of Moloney murine leukemia

129 ts1 is a temperature-sensitive mutant of Moloney murine leukemia

130 omyelopathy caused by ts1, a neuropathogenic temperature-sensitive mutant of Moloney murine leukemia

131 Rat fibroblasts transformed by a temperature-sensitive mutant of murine p53 undergo a rev

132 o induce second-site reversion of the Val135 temperature-sensitive mutant of murine p53.

133 A temperature-sensitive mutant of Mycobacterium smegmatis

134 We have characterized SMEG53, a temperature-sensitive mutant of Mycobacterium smegmatis

135 We have isolated a UV-induced temperature-sensitive mutant of Mycobacterium smegmatis

136 and in 32D cells constitutively expressing a temperature-sensitive mutant of p53 (32Dtsp53), overexpr

137 By using a temperature-sensitive mutant of p53 where cytoplasmic-nu

138 in extracts of a cell line that expresses a temperature-sensitive mutant of p53.

139 n by VhD mouse fibroblast cells expressing a temperature-sensitive mutant of p53.

140 ysis by microarray (dSLAM) using PDIa' and a temperature-sensitive mutant of PDIa' as query mutations

141 Using a temperature-sensitive mutant of Pkc1p revealed a high le

142 tiple growth-inhibitory properties of pRb, a temperature-sensitive mutant of pRb has been produced.

143 ctor RCC1 in the tsBN2 cell line harboring a temperature-sensitive mutant of RCC1 blocked NF-kappaB a

144 We have isolated and characterized a temperature-sensitive mutant of Schizosaccharomyces pomb

145 We have used a transport-defective, temperature-sensitive mutant of Sindbis virus, ts23, whi

146 ) of Saccharomyces cerevisiae, we isolated a temperature-sensitive mutant of SSA1.

147 immortalized with a retrovirus expressing a temperature-sensitive mutant of SV40-large T antigen (ts

148 n of TAFII250 in ts13 cells, which express a temperature-sensitive mutant of TAFII250, leads to the i

149 With a temperature-sensitive mutant of the CDC23 subunit of the

150 rvals in filaments generated by the use of a temperature-sensitive mutant of the cell division gene d

151 n the study reported here, we used the tsO45 temperature-sensitive mutant of the G protein of vesicul

152 ested by overexpressing the SMF1 gene in the temperature-sensitive mutant of the mitochondrial proces

153 Using a temperature-sensitive mutant of the p210 BCR-ABL gene, t

154 ly, concomitant with maximal activation of a temperature-sensitive mutant of the PTK.

155 nactivation of the immortalizing oncogene, a temperature-sensitive mutant of the simian virus 40 larg

156 L75F-TrpR is a temperature-sensitive mutant of the tryptophan repressor

157 A temperature-sensitive mutant of the V. cholerae generali

158 By use of a temperature-sensitive mutant of twist, we show that acti

159 Using ts20 cells, which express a temperature-sensitive mutant of ubiquitin-activating enz

160 Use of a temperature-sensitive mutant of ubiquitin-conjugating en

161 Using normal rat kidney cells expressing a temperature-sensitive mutant of v-Src, we examined the r

162 lycoprotein E1-G or E2-G on the surface of a temperature-sensitive mutant of vesicular stomatitis vir

163 line expressing E2-G or E2DeltaHVR1-G with a temperature-sensitive mutant of VSV (VSVts045), displaye

164 s were induced in pre-B cells transformed by temperature-sensitive mutants of Abelson murine leukemia

165 end, we have used pre-B cells transformed by temperature-sensitive mutants of Abelson virus that unde

166 Temperature-sensitive mutants of ACA were selected from

167 Temperature-sensitive mutants of Actinobacillus pleuropn

168 ic spindle and to kinetochores and generated temperature-sensitive mutants of DAD2 and ASK1.

169 d silencing in S. pombe, we analyzed several temperature-sensitive mutants of DNA polymerase alpha.

170 In the nematode Caenorhabditis elegans, temperature-sensitive mutants of emb-1 arrest as one-cel

171 Functional analysis of temperature-sensitive mutants of hspd1 and mps1 revealed

172 Studies using temperature-sensitive mutants of Kin28 have provided the

173 After a jump to 32 degrees C, temperature-sensitive mutants of kinesin-2 (fla10) showe

174 In a screen for temperature-sensitive mutants of Saccharomyces cerevisia

175 We screened a collection of temperature-sensitive mutants of Saccharomyces cerevisia

176 A screen for temperature-sensitive mutants of Saccharomyces cerevisia

177 A screen was designed to identify temperature-sensitive mutants of Saccharomyces cerevisia

178 are small, we isolated and characterized 11 temperature-sensitive mutants of TAF90 and analyzed thei

179 Temperature-sensitive mutants of TFIIB that are defectiv

180 Cell lines expressing temperature-sensitive mutants of the tumor suppressor pr

181 e forms of TLK-1 suppresses the lethality of temperature-sensitive mutants of the yeast Aurora B kina

182 Using temperature-sensitive mutants of TIF34 we show that this

183 Here, using temperature-sensitive mutants of trz1 and trz2, we provi

184 Studies with temperature-sensitive mutants of v-abl show that this gr

185 Temperature-sensitive mutants of vaccinia virus, with ge

186 Temperature-sensitive mutants of Vti1p show a similar ca

187 Mitochondria isolated from an mmm1-1 temperature-sensitive mutant or from an mdm10 deletion m

188 We used temperature-sensitive mutants or a conditional degradati

189 (p92), was observed to form a complex with a temperature-sensitive mutant p53 (TSp53(Val-135)) in the

190 ivation in cultured human cells, either by a temperature-sensitive mutant p53 protein or by gamma-irr

191 sion of several genes was dependent on a non-temperature-sensitive mutant p53 suggesting altered tran

192 idney (BRK) cells transformed with E1A and a temperature-sensitive mutant p53 using a PCR-based subtr

193 Expression of a temperature sensitive mutant p53Val(135) sensitizes COS-

194 the wild-type gene by complementation of the temperature-sensitive mutant phenotype.

195 n mitotic spindle formation; (ii) the RanGEF temperature-sensitive mutant pim1-d1 has abnormal actin

196 not affected by thermal inactivation of the temperature-sensitive mutant Polalpha (pol1-17), but was

197 as reduced after thermal inactivation of the temperature-sensitive mutant Poldelta (pol3-1) or Polvar

198 hal mutant proteins only two, as well as one temperature-sensitive mutant protein, were incorporated

199 y proteins, peptides and antisense RNAs, and temperature-sensitive mutant proteins.

200 A Schizosaccharomyces pombe temperature-sensitive mutant, rae1-1, was previously ide

201 Loss of Ags1p function in a temperature-sensitive mutant results in cell lysis, wher

202 y a string motif whose disruption in a sec14 temperature-sensitive mutant results in destabilization

203 Sequencing of the temperature-sensitive mutant revealed a 21-bp duplicatio

204 Soluble fractions prepared from temperature-sensitive mutants revealed requirements for

205 Further analysis of these temperature-sensitive mutants revealed that each display

206 A temperature-sensitive mutant, sec34-2, is defective in t

207 Some of the temperature-sensitive mutants showed reduction in the mi

208 stantially in wild-type E.coli, but not in a temperature sensitive mutant strain in RNase P at the re

209 on of hydroxyurea or by genetic control in a temperature-sensitive mutant strain defective in DNA syn

210 for this phenotype, we found that shifting a temperature-sensitive mutant strain to 42 degrees C led

211 ME49 strain or, surprisingly, an attenuated temperature-sensitive mutant strain, ts4.

212 constructed double mutants of Deltamyp2 with temperature-sensitive mutant strains defective in cytoki

213 Temperature-sensitive mutant strains generated were used

214 pidly after loss of Grd20p function in grd20 temperature-sensitive mutant strains, indicating that Gr

215 er with the present characterization of A20R temperature-sensitive mutants suggested that the A20R, D

216 prAPI to the membrane and analysis of a cvt9 temperature-sensitive mutant supports a direct role of C

217 MC alpha-actin promoter was used to target a temperature-sensitive mutant SV40 T antigen (tsA58) to s

218 sms in Saccharomyces cerevisiae identified a temperature-sensitive mutant that displayed phenotypes c

219 The ts-4 strain of Toxoplasma gondii is a temperature-sensitive mutant that fails to grow at 40 de

220 We identified temperature-sensitive mutants that accumulated several d

221 DC55, PPH21, or PPH22 is also toxic to other temperature-sensitive mutants that display defects in mi

222 8+ was originally identified in a screen for temperature-sensitive mutants that exhibit a cell-divisi

223 s underlying age asymmetry, we have isolated temperature-sensitive mutants that have limited growth c

224 However, in the temperature-sensitive mutant, the insertion of the Sec-i

225 vity was blocked, either with a drug or in a temperature-sensitive mutant, the knotted recombination

226 Under nonpermissive conditions, temperature-sensitive mutants (ts2 and ts25) that map to

227 Temperature-sensitive mutant ts23 of Sindbis virus conta

228 antibody-neutralized wild-type virus and the temperature-sensitive mutant tsB7, the high level of JNK

229 e characterized the growth of a B1-deficient temperature-sensitive mutant virus (Cts2 virus) in U2OS

230 with wild-type poliovirus than in cells with temperature-sensitive mutant virus that contains a mutat

231 In the current study, a novel temperature-sensitive mutant virus was shown to contain

232 A lipB temperature-sensitive mutant was shown to produce a ther

233 We now report a temperature-sensitive mutant (WD2) with an A270T substit

234 oteins; again, because of lack of a suitable temperature-sensitive mutant, we were unable to test whe

235 ects found in specific strains of yeast vti1 temperature-sensitive mutants, we show that AtVTI1a can

236 Using this collection of temperature-sensitive mutants, we show that in all cases

237 Both temperature-sensitive mutants were also found to be mark

238 Two temperature-sensitive mutants were characterized in deta

239 Temperature-sensitive mutants were defective in RNA repl

240 Six temperature-sensitive mutants were isolated via random m

241 Temperature-sensitive mutants were obtained through sing

242 ep protein is inhibited in a short time in a temperature-sensitive mutant when shifted to the nonperm

243 duction of PINA levels exacerbates the nimA5 temperature-sensitive mutant, whereas overexpression of

244 We report here additional data on 17 other temperature sensitive mutants which are in the beta coil

245 sion of a previously isolated vaccinia virus temperature-sensitive mutant which forms multilayered en

246 YEF3 defect in a diploid null mutant and two temperature-sensitive mutants which have been shown prev

247 s is consistent with data from other exocyst temperature-sensitive mutants, which disrupt the integri

248 ween Ost3p and Stt3p in the presence of ost4 temperature-sensitive mutants, which indicates Ost4p, vi

249 not acquire TX-100 insolubility in ts 56 (a temperature-sensitive mutant with a defect in NP protein

250 Another variant FliN from a temperature-sensitive mutant with a different phenotype

251 actor for virion morphogenesis by studying a temperature-sensitive mutant with a lesion in A6.

252 A temperature-sensitive mutant with a lesion in the I7L ge

253 The p53 mutant 143Ala is a human temperature-sensitive mutant with two conformational sta

254 he accompanying report, which documents that temperature-sensitive mutants with lesions in the A20 ge

255 espite extensive efforts over many years, no temperature-sensitive mutants with mutations in the stru

256 Two BMV (temperature-sensitive) mutants with alterations in the 2

257 spatial and temporal control of Filamenting temperature sensitive mutant Z (FtsZ) Z-ring formation i

258 direct inhibitor of the E. coli filamenting temperature-sensitive mutant Z division protein.

259 eukaryotic tubulin and bacterial filamenting temperature-sensitive mutant Z protein (FtsZ) polymers.

260 thesized Gp0.4 binds to purified filamenting temperature-sensitive mutant Z protein and directly inhi