ライフサイエンスコーパス: terbium

1 al probing method using the lanthanide metal terbium.

2 Experiments using terbium, a fluorescent calcium analogue, confirm the spe

3 Terbium, a lanthanide ion, blocked motility even when ca

4 hate interference 1000-fold compared to free terbium alone.

5 trypsin; loss of time-gated FRET between the terbium and QD signaled the activity of trypsin.

6 ubstrate shows a high degree of FRET between terbium and YFP, whereas DUB-dependent cleavage leads to

7 The properties of the gadolinium, terbium, and dysprosium complexes of these ligands were

8 his TnT isoform was first investigated using terbium as a calcium analogue due to its more readily de

9 encoded lanthanide-binding tag (LBT) to bind terbium as a LRET donor and a fluorophore-labeled iberio

10 A terbium-based complex that displays a water exchange CES

11 strophin monoclonal antibody conjugated to a terbium-based resonance energy transfer donor and anti-a

12 Thus, a strategy of using terbium-based TR-FRET can be applied to develop kinase a

13 Using this three-channel terbium-based, TR-FRET assay system, we show in one expe

14 In an intact probe, the average terbium-BHQ distance is short, and Tb --> BHQ energy tra

15 terbium/BHQ probes is very dependent on the terbium-BHQ distance.

16 Luminescence quenching efficiency within terbium/BHQ probes is very dependent on the terbium-BHQ

17 Terbium binding and isothermal titration calorimetry dat

18 nds 4 mol of calcium/mol of protein and that terbium-binding stoichiometry is similar to that of calc

19 unprocessed serum, by taking advantage of a terbium chelate complex with long luminescence lifetime

20 After a brief excitation pulse, terbium chelates emit for milliseconds after the intrins

21 ntage of the long phosphorescent lifetime of terbium chelates, a property that enables the accurate d

22 iC stem minihelix, as evidenced by increased terbium cleavage in this domain.

23 At low concentrations of NC, the strong terbium cleavage observed in the core region of the tRNA

24 ntroduced into P4 cause local changes in the terbium cleavage pattern due to alternate metal ion-bind

25 In light of the alternate terbium cleavage pattern in P4 caused by bulge deletion,

26 nformation at low Mg(2+) concentrations, and terbium-cleavage assays suggest that this increase is du

27 The resulting terbium coiled coil displays luminescent properties cons

28 onstituents of the FRET relay, a luminescent terbium complex and fluorescent dye, were assembled to Q

29 LRET between the eDHFR-bound terbium complex and green fluorescent protein (GFP) was

30 A stable and highly luminescent terbium complex based on a tetraisophthalamide (TIAM) ch

31 he four luminescent lanthanides studied, the terbium complex exhibits the greatest dipicolinate bindi

32 ied with the aid of the W220Y mutant and the terbium complex of the apoenzyme.

33 s to reversal of the magnetization, and a di-terbium complex that displays magnetic hysteresis up to

34 A luminescent terbium complex, TMP-Lumi4, was introduced into cultured

35 residues were employed to anchor luminescent terbium complexes and biotin groups based on orthogonal

36 Luminescent terbium complexes are an example of such a material, and

37 of the particle, as an energy acceptor, and terbium complexes as an energy donor.

38 ss large energy barriers, and dysprosium and terbium complexes bridged by an N2(3-) radical ligand ex

39 While terbium complexes were used as luminescent signaling gro

40 d to a number of different proteins, and the terbium complexes' exceptional photophysical properties

41 of single-molecule-magnet behavior, with the terbium congener exhibiting magnetic hysteresis at 14 K

42 re linked to metal ion interactions, we used terbium-dependent cleavage of the phosphate backbone to

43 Our calculations also show that terbium dioxide has a high electronic entropy and thus c

44 Moreover, TprC(Fl) increased efflux of terbium-dipicolinic acid complex from large unilamellar

45 Using terbium-dipicolinic acid complex-loaded large unilamella

46 the three acceptors at the four channels for terbium donor emission, we demonstrate that any of these

47 organic acceptor fluorophores paired with a terbium donor fluorophore, we have developed the first e

48 uorescent protein (GFP) can be paired with a terbium donor in a TR-FRET assay, we have developed TR-F

49 cent protein (YFP) and a chemically attached terbium donor.

50 magnitude of the three FRET signals in this terbium-donor triple-acceptor system with minimal bleedt

51 rth elements (yttrium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium

52 is lanthanum, cerium, samarium, gadolinium, terbium, dysprosium, thulium, or calcium.

53 raacetic acid) based on a highly fluorescent terbium-EDTA-salicylic acid complex formation was develo

54 y transfer is efficient, decreasing both the terbium emission intensity and lifetime.

55 hree repeats using changes in tryptophan and terbium fluorescence and perturbation of [1H-15N]-HSQC N

56 ophan fluorescence and tryptophan-sensitized terbium fluorescence indicate that the calcium binding s

57 T assays involve a TRE sequence labeled with terbium (fluorescence donor), TRbeta.RXRalpha heterodime

58 Since terbium has been used extensively as a probe for the det

59 the basis of enhancement of the emission of terbium (III) ions.

60 luorescence emission of the lanthanide metal terbium(III) (Tb(3+)) when it interacts with the aromati

61 Terbium(III) [Tb(III)] was shown to inhibit the hammerhe

62 s regard by using lanthanide centres such as terbium(III) and dysprosium(III), whose anisotropy can l

63 ecursor and product we observe high-affinity terbium(III) binding sites in joining sequence J4/2 (Tb(

64 eled with either a long lifetime luminescent terbium(III) complex (Tb) or a fluorescent dye, Alexa Fl

65 otors, based on the sensitized emission of a terbium(III) complex.

66 We show, using terbium(III) footprinting and sensitized luminescence sp

67 Terbium(III) footprinting detects conformations for the

68 d subtle, yet significant differences in the terbium(III) footprinting pattern between the precursor

69 uorescence spectroscopy, circular dichroism, terbium(III) footprinting, and X-ray crystallography of

70 rosine kinase-inducible domain peptides bind terbium(III) in a phosphorylation-dependent manner, show

71 Here, we have used the lanthanide metal ion terbium(III) to footprint the precursor and product solu

72 Here, we augment these studies by using terbium(III) to probe the structure of the trans-acting

73 ynergizing the strong magnetic anisotropy of terbium(III) with the effective exchange-coupling abilit

74 aqueous complex of the lanthanide metal ion terbium(III), Tb(OH)(aq)(2+), reversibly inhibit the rib

75 nosalicylic acid ethylenediaminetetraacetate terbium(III), were evaluated for the analysis of carboni

76 Terbium ion (Tb3+), a fluorescent trivalent cation that

77 e, as revealed by sensitivity to cleavage by terbium ion and by the ability of the internal loop to d

78 combined the favorable characteristics of a terbium-ion-containing lanthanide-binding peptide (Tb(3+

79 ranes coupled with anomalous scattering from terbium ions (Tb3+) titrated into presumed Ca2+ binding

80 Studies of hypothetical praseodymium(IV) and terbium(IV) analogues suggest the inverse-trans-influenc

81 d such that each myosin molecule contained a terbium-labeled (luminescent donor) RLC on one head and

82 gged ERRgamma ligand-binding domain (LBD), a terbium-labeled anti-GST antibody, a fluorescein-labeled

83 ion or phosphorylation using a unique set of terbium-labeled antibodies in a time-resolved Forster re

84 an increase in TR-FRET when incubated with a terbium-labeled antibody that specifically recognizes th

85 estrogen from the ligand binding pocket of a terbium-labeled estrogen receptor, at the same time caus

86 rt a powerful alternative (GlycoFRET), where terbium-labeled fluorescent reporters are irreversibly a

87 rresponding to a final concentration of 6 pM terbium-labeled probes detectable by ICP-MS after elutio

88 kinase assays, and the unique properties of terbium lead to a high degree of flexibility with regard

89 onal cation binding site as assessed by both terbium luminescence and electrospray ionization mass sp

90 adhesion site (MIDAS) motif, was assessed by terbium luminescence to evaluate conformational perturba

91 um luminescence when phosphorylated but weak terbium luminescence when not phosphorylated.

92 phorylation-dependent manner, showing strong terbium luminescence when phosphorylated but weak terbiu

93 phatase YOP resulted in a large reduction in terbium luminescence.

94 h Abl kinase resulted in a large increase in terbium luminescence.

95 Full-length calbindin D28K bound 4 mol of terbium/mol of protein, while calbindin delta2 and delta

96 Terbium offers 4 clinically interesting radioisotopes wi

97 Since terbium often substitutes at calcium-binding sites, radi

98 l usefulness of the combined use of laccase, terbium oxide nanoparticles (Tb4O7NPs) and 8-hydroxypyre

99 e-molecule magnets: recent studies show that terbium phthalocyanine complexes possess large energy ba

100 opy with a selectively targeted, luminescent terbium protein label affords improved speed and sensiti

101 proof-of-concept study was to produce all 4 terbium radioisotopes and assess their diagnostic and th

102 Carrier-free terbium radioisotopes were obtained after purification,

103 greater than 96% radiochemical yield for all terbium radioisotopes.

104 For the first time, to our knowledge, 4 terbium radionuclides have been tested in parallel with

105 -Tb separation of 3.9020(10) A for the major terbium sites.

106 ffect of lanthanide cofactors, in particular terbium (Tb(3+)), for DNA-catalyzed synthesis of 2',5'-b

107 content along with another REE, particularly terbium (Tb).

108 We have employed 45CaCl2 binding studies, terbium (Tb3+) luminescence spectroscopy, and electrospr

109 ly complex lanthanide ions and can sensitize terbium (Tb3+) luminescence.

110 ng proteins, we also examined the binding of terbium to the three proteins under the same conditions.

111 c acid did not form fluorescent complex with terbium under the same conditions.

112 Using the fluorescent calcium analog terbium, we found that heparinase I tightly bound divale

113 and TnT N-terminal fragment (TnT N47) bound terbium with high affinity indicating that the N-termina

114 All five mutants bound terbium with unaltered affinities.