ライフサイエンスコーパス: terminase

1 equivalents of L-terminase (1S-terminase:2L-terminase).

2 ase activity to a level comparable with FL-L-terminase.

3 rminal domain for interacting with the large terminase.

4 onucleolytic activity in the context of FL-L-terminase.

5 med capsids through the actions of the viral terminase.

6 nus and at 5.7 nm from the C terminus of the terminase.

7 al domains required for interaction with the terminase.

8 fic terminase and reduce packaging by lambda-terminase.

9 ocess that requires ATP hydrolysis by lambda terminase.

10 ty shell, prohead, and the packaging enzyme, terminase.

11 he concatemer-cutting endonuclease reside in terminase.

12 )28-pU(L)33 complex to form the likely viral terminase.

13 ical for structure and function of the small terminase.

14 odimeric viral DNA packaging protein, called terminase.

15 basis for a minimal kinetic model for lambda terminase.

16 inase and human cytomegalovirus (HCMV) small terminase.

17 binds specifically to the ATPase domain of L-terminase.

18 tor rather than the nuclease activity of the terminase.

19 nd between GFP portal and single dye-labeled terminases.

20 ic S-terminase bound to two equivalents of L-terminase (1S-terminase:2L-terminase).

21 bound to two equivalents of L-terminase (1S-terminase:2L-terminase).

22 A translocation is accomplished by the phage terminase, a powerful molecular motor consisting of larg

23 Genome maturation is carried out by the terminase, a protein complex that mediates both insertio

24 sence or absence of genes coding for a phage terminase (ACICU_02185), a sialic acid synthase (ACICU_0

25 We further show that lambda terminase adopts a heterogeneous mixture of higher-order

26 Genome maturation is carried out by the terminase, an enzyme complex that mediates both the inse

27 fied in herpes simplex virus 1 (HSV-1) large terminase and human cytomegalovirus (HCMV) small termina

28 r the specific endonuclease activity of HK97 terminase and is essential for phage head morphogenesis.

29 cause enhanced DNA packaging by 21-specific terminase and reduce packaging by lambda-terminase.

30 The Xfas53 terminase and structural genes are related at a protein

31 reported, revealing the coupling between the terminase and the connector forming a continuous channel

32 ive site, which are conserved in herpesvirus terminases and bear great similarity to the phage T4 gp1

33 data define the N-terminal ATPase center in terminases and show for the first time that subtle defec

34 The packaging machine consists of a "small terminase" and a "large terminase" component.

35 two genes are the two subunits of the HCMV "terminase" and are necessary for cleavage and packaging

36 d (gp23), portal (gp20), motor (gp17, large "terminase"), and regulator (gp16, small terminase), lead

37 8 kDa; small terminase), gp17 (70 kDa; large terminase), and gp20 (61 kDa; dodecameric portal).

38 er of proteins, including the integrase, the terminase, and two major structural proteins.

39 resolution provides the first glimpse of P22 terminase architecture and implies two distinct modes of

40 pUL6 and the associated viral terminase are required for processing of concatemeric vi

41 Terminases are enzymes common to complex double-stranded

42 The structure of the terminase assembled into the complex showed a different

43 Terminase assembly at the packaging initiation site is r

44 ite with which a molecular motor, termed the terminase, associates during the DNA packaging reaction.

45 dentify a portal protein domain critical for terminase association with the capsid and suggest that b

46 and C-terminal nuclease domain, suggest that terminase association with the prohead portal and commun

47 inase morphological change, we generated the terminase atomic model based on the crystallographic str

48 ng studies revealed that the N-terminus of L-terminase ATPase domain (residues 1-58) contains a minim

49 l HTH motifs of the Bacillus phage SF6 small terminase bind the packaging regions of SF6 and related

50 domain (residues 1-58) contains a minimal S-terminase binding domain sufficient for stoichiometric a

51 with residues 140-162 of S-terminase, the L-terminase binding domain.

52 eaving a binary complex consisting of lambda terminase bound to the head end of the adjacent genome.

53 predominantly of one copy of the nonameric S-terminase bound to two equivalents of L-terminase (1S-te

54 leavage and packaging functions of the viral terminase, but not for terminase complex assembly.

55 ave determined the structure of the T7 large terminase by electron microscopy.

56 own a decrease in distance from the phage T4 terminase C terminus to portal consistent with a linear

57 Mutations in the phage T4 terminase C-motif lead to loss of stimulated ATPase and

58 egion showed that all phages had related DNA terminase, C1 repressor and DNA recombinase genes.

59 This supports a model in which ES18 terminase can move substantial distances along the DNA b

60 ifferent functional classes of phage-encoded terminases can usually be predicted from their amino aci

61 taining two 21-bp direct repeats and a major terminase cleavage site in the phage genome.

62 alf of gp17 is thought to be involved in the terminase cleavage.

63 Thus we postulate that viral terminases cleave DNA by the canonical RuvC-like mechani

64 rst direct visualization of a purified viral terminase complex analyzed in the absence of DNA and pro

65 gp1 in formation of the packaging-competent terminase complex and assembly of the terminase with the

66 unctions of the viral terminase, but not for terminase complex assembly.

67 The human cytomegalovirus terminase complex cleaves concatemeric genomic DNA into

68 product 20 (gp20, 61 kDa) and an oligomeric terminase complex composed of gp16 (18 kDa) and gp17 (70

69 The human cytomegalovirus (HCMV) terminase complex consists of several components acting

70 mass spectrometry revealed that the purified terminase complex consists predominantly of one copy of

71 ication, indicating that drugs targeting the terminase complex could be safe and selective.

72 late time point, similarly to other reported terminase complex inhibitors.

73 The structure of the complete connector-terminase complex is also reported, revealing the coupli

74 The herpesviral terminase complex is part of the intricate machinery tha

75 The tripartite terminase complex of herpesviruses assembles in the cyto

76 ds milligram quantities of the S-terminase:L-terminase complex of the Salmonella phage P22.

77 In vivo assembled terminase complex was affinity-purified and stabilized b

78 One component of the human cytomegalovirus terminase complex, pUL89, provides the endonucleolytic a

79 known about the architecture of an assembled terminase complex.

80 mpered by the inability to purify the intact terminase complex.

81 nitiated through interaction between prohead-terminase complexes and transcription elongation complex

82 aracteristics of proteins that make up viral terminase complexes to identify or design additional ter

83 In this study, terminase complexes were isolated by tandem-affinity pur

84 n of codons 400 to 420 of U(L)15, encoding a terminase component, was analyzed.

85 consists of a "small terminase" and a "large terminase" component.

86 The varicella-zoster virus (VZV) terminase components (pORF25, pORF30, and pORF45/42) hav

87 conclude that interactions between putative terminase components are tightly linked to successful vi

88 portal protein encoded by UL6, the putative terminase components encoded by UL15, UL 28, and UL33, t

89 Whether putative terminase components localized in the nucleus or cytopla

90 kaging other than its interaction with other terminase components.

91 Terminases comprise essential components of molecular mo

92 The existence of two terminase conformations and its possible relation to the

93 The docking of the threaded model in both terminase conformations showed that the transition betwe

94 Terminases consisting of two to three packaging-related

95 The data suggest that the T4 terminase consists of at least two functional domains, a

96 The terminase contains a large subunit that is thought to cl

97 show that either ATP or ADP is required for terminase cutting at cos, to generate the active, DNA pa

98 ing on "endless" concatemeric DNA in vivo by terminase depends upon interaction with the DNA loaded g

99 th respect to the assembly and function of a terminase DNA-packaging motor are discussed.

100 veals a common scheme for oligomerization of terminase DNA-recognition components, and provides insig

101 The finding that the portal and terminase do eventually interact was supported by the ob

102 ion motors consist of a multimeric packaging terminase docked onto a unique procapsid vertex containi

103 The terminase docks onto the portal protein complex embedded

104 establish the function for each of the small terminase domains.

105 scuss the model in relation to proposals for terminase-driven DNA translocation in other phages.

106 A novel terminase-driven mechanism is proposed for translocation

107 Many dsDNA viruses encode DNA-packaging terminases, each containing a nuclease domain that resol

108 distinct, it appears that T4 and other phage terminases employ a common catalytic paradigm for phosph

109 revealed that numerous phage and viral large terminases encode a common Walker-B motif in the N-termi

110 These mutant enzymes retained the terminase endonuclease and helicase activities, but had

111 l vertex, and all components of a tripartite terminase enzyme are required to both cleave and package

112 Terminase enzyme complexes, which facilitate ATP-driven

113 bda genome into the viral capsid, the lambda terminase enzyme introduces symmetric nicks, 12 bp apart

114 As the terminase enzyme packages the genome into the phage caps

115 and U(L)28 are believed to form part of the terminase enzyme, a protein complex essential for the cl

116 In addition to the terminase enzyme, the packaging chaperone, FI protein (g

117 and herpes viruses require the activity of a terminase enzyme, which is comprised of large and small

118 DNA is packaged into a procapsid shell by a terminase enzyme.

119 preformed procapsid structure, catalyzed by terminase enzymes and fueled by ATP hydrolysis.

120 Terminase enzymes are common to both eukaryotic and prok

121 Terminase enzymes are common to both prokaryotic and euk

122 Terminase enzymes are common to double-stranded DNA (dsD

123 Terminase enzymes are responsible for "packaging" of vir

124 Terminase enzymes are viral motors that package DNA into

125 to an empty procapsid, and it is likely that terminase enzymes from disparate viruses utilize a commo

126 enomes linked in a head-to-tail fashion, and terminase enzymes perform two essential functions: 1) ex

127 Viral terminase enzymes serve as genome packaging motors in ma

128 This work is thus of relevance to all terminase enzymes, both prokaryotic and eukaryotic.

129 ntains the nuclease and ATPase activities of terminase, exists as a stable monomer with an alpha/beta

130 In contrast, small terminase exposes a classical NLS at the far C terminus

131 ined by in-drop proteolysis of full-length L-terminase (FL-L-terminase) reveals a central seven-stran

132 a monopartite NLS at the N terminus of large terminase, flanking the ATPase domain, that is conserved

133 Escherichia coli MltG (YceG) as a potential terminase for glycan polymerization that is broadly cons

134 We find that purified, recombinant lambda terminase forms a homogeneous, heterotrimeric structure,

135 The crystal structure of the large terminase from the Geobacillus stearothermophilus bacter

136 different oligomerization states for a small terminase from the T4 family of phages.

137 ed among herpesviruses are also conserved in terminases from DNA bacteriophage.

138 sis reveals that the role of HNH proteins in terminase function is widespread among long-tailed phage

139 y conserved region (region IX) essential for terminase function.

140 uence despite the selective pressure to keep terminase gene products active and localized in the nucl

141 The Sf6 terminase genes are unusual.

142 e (HNHE) next to their cohesive end site and terminase genes.

143 cularly permuted genome that begins with the terminase genes.

144 y a variety of factors; Mg, NaCl, ATP, small terminase gp16 and N-terminal ATPase domain.

145 nase motor (gp17), and 11- or 12-meric small terminase (gp16).

146 nuclease activity is modulated by the 'small terminase', gp16, by the N-terminal ATPase domain of gp1

147 An 18-kDa small terminase, gp16, is also essential, but its role in DNA

148 packaging assay employed purified proheads, terminase (gp17 + gp16), and ATP to encapsidate DNA resi

149 hydrolysis in the pentameric phage T4 large "terminase" (gp17) motor.

150 , present in the C-terminal domain of 'large terminase' gp17, has been defined by mutational, biochem

151 h resides in the C-terminal domain of large 'terminase' gp17, is a weak endonuclease and regulated by

152 ted by gene product 16 (gp16) (18 kDa; small terminase), gp17 (70 kDa; large terminase), and gp20 (61

153 e phage T4 motor, a pentamer of 70-kDa large terminase, gp17, is the fastest and most powerful motor

154 we show that residue K428 of a bacteriophage terminase gp2 nuclease domain mediates binding of the me

155 ucture and regulation of bacteriophage P22 L-terminase (gp2).

156 While the enzymology of lambda-terminase has been well described, the nature of the cat

157 The terminases have an ATPase center located in the N termin

158 packaging experiments show that these mutant terminases have lost the ability to discriminate between

159 eneral interest, given the parallels between terminases, helicases, and other motor proteins.

160 We report that the ATPase motors of terminases, helicases, translocating restriction enzymes

161 e defects in virion assembly, and lacked the terminase high-affinity ATPase activity.

162 Bacteriophage lambda terminase holoenzyme is a hetero-oligomer composed of th

163 results with respect to the two roles of the terminase holoenzyme, DNA maturation and DNA packaging,

164 nal on viral DNA and directs assembly of the terminase holoenzyme.

165 finger domain found in all herpesvirus ORF30 terminase homologs but also identified a novel, highly c

166 ease function of human cytomegalovirus (HCMV terminase) in vitro.

167 wne-infected cultures treated with the viral terminase inhibitor 2-bromo-5,6-dichloro-1-beta-d-ribofu

168 ch that combines treatment with an antiviral terminase inhibitor and purification by a simplified pro

169 rus drugs include viral helicase-primase and terminase inhibitors.

170 s are believed to encode two subunits of the terminase involved in cleavage and packaging of viral ge

171 The terminase is a powerful motor that converts ATP hydrolys

172 Herpes simplex virus (HSV) terminase is an essential component of the molecular mot

173 The HSV terminase is believed to consist of the UL15, UL28, and

174 Bacteriophage lambda terminase is composed of two subunits, gpA and gpNu1, in

175 During translocation, terminase is docked on the prohead's portal protein.

176 utant UL89 and UL56 proteins, since the HCMV terminase is likely to interact with the portal protein

177 aging of the viral genome, whereas the large terminase is responsible for the ATP-powered translocati

178 One of the functions of the small terminase is to initiate packaging of the viral genome,

179 Phage lambda with the chimeric terminase is unable to form plaques, but pseudorevertant

180 NA packaging enzyme of bacteriophage lambda, terminase, is a heteromultimer composed of a small subun

181 terminase (S-terminase) subunit and a large terminase (L-terminase) subunit, transiently docked at t

182 ocessing nuclease, both located in the large terminase (L-terminase) subunit.

183 em that yields milligram quantities of the S-terminase:L-terminase complex of the Salmonella phage P2

184 In addition, using active terminases labeled at the N- and C-terminal ends with a

185 We propose a scheme for the roles of P22 terminase large and small subunits in the recruitment an

186 of ectopically expressed and highly purified terminase large and small subunits.

187 ate for interaction with and cleavage by the terminase large subunit prior to DNA translocation into

188 ay structure of the C-terminal domain of the terminase large-subunit pUL15 (pUL15C) from HSV-1.

189 rge "terminase"), and regulator (gp16, small terminase), leading to precise orchestration of the pack

190 In HSV, the terminase likely comprises the U(L)15, U(L)28, and U(L)3

191 We show that the activity of gp74 in terminase-mediated cleavage of the phage cos site relies

192 To understand in molecular terms the terminase morphological change, we generated the termina

193 dodecameric portal (gp20), pentameric large terminase motor (gp17), and 11- or 12-meric small termin

194 Here, we present mechanistic studies on the terminase motor from bacteriophage lambda.

195 Many viruses use a powerful terminase motor to pump their genome inside an empty pro

196 face of the expanded capsid lattice, and the terminase motors tightly package DNA, generating up to a

197 cribed in this study represent the first VZV terminase mutants reported to date.

198 ntercalating dyes arrest packaging, but rare terminase mutations confer resistance.

199 nt herpesviruses to regulate the kinetics of terminase nuclear import, reflecting a mechanism of viru

200 Crystal structures of the large terminase nuclease from the thermophilic bacteriophage G

201 Terminase nucleases resemble the RNase H-superfamily nuc

202 The terminase of herpes simplex virus (HSV) is composed of t

203 zed the interactions between small and large terminases of T4-related phages.

204 The mechanism by which a ring-shaped small terminase oligomer binds viral DNA has not previously be

205 Packaging with a C-terminal fluorescent terminase on a GFP portal prohead, FRET shows a reductio

206 Bacteriophage terminases package DNA through the portal ring of a proc

207 etent for high affinity binding to the large terminase packaging protein, and a symmetric ring in the

208 conformation when compared with the isolated terminase pentamer.

209 residue, and that the large subunit of HK97 terminase physically interacts with gp74.

210 Viral terminases play essential roles as components of molecul

211 sembly and DNA packaging genes, which encode terminase, portal, scaffold, and coat proteins, are extr

212 Remarkably, terminases, portal proteins, and shells of tailed bacter

213 Bacteriophage lambda terminase possesses a site-specific nuclease activity, a

214 s-driven conformational changes of portal or terminase powering DNA motion.

215 substrate did not remain stably bound by the terminase-prohead.

216 teriophage T4 motor, a pentamer of the large terminase protein (gp17) assembled at the portal vertex

217 rmed between the empty prohead and the large terminase protein (gp17) that can capture and begin pack

218 g of only two components, proheads and large terminase protein (gp17; 70 kDa), is constructed.

219 oteins closely related to coliphage lambda's terminase protein (the large subunit).

220 The crystal structure of a full-length small terminase protein from the Siphoviridae bacteriophage SF

221 rminal ATPase site of bacteriophage T4 large terminase protein gp17 is critically required for DNA pa

222 Previous evidence suggests that the large terminase protein gp17, a key component of the T4 packag

223 The small terminase protein is essential for the initial recogniti

224 n phage T4, there is evidence that the large terminase protein, gene product 17 (gp17), assembles int

225 The small terminase protein, gp16 (18 kDa), is not only not requir

226 The small terminase protein, gp16, inhibited DNA binding, which wa

227 l terminase protein, gpl6 (18kDa), the large terminase protein, gp17 (70kDa), and the dodecameric por

228 The phage T4 motor is composed of the small terminase protein, gpl6 (18kDa), the large terminase pro

229 An essential part of this machine, the large terminase protein, processes viral DNA into constituent

230 tal protein and two non-structural packaging/terminase proteins assembled at the unique portal vertex

231 Biochemical studies of the terminase proteins have been hampered by the inability t

232 Viral pac site-targeting small terminase proteins possess an unusual architecture in wh

233 activity of the Terminase_1 family of large terminase proteins.

234 We show that IHF and the terminase protomer cooperatively assemble at the cos sit

235 The lambda-terminase protomer is composed of one large catalytic su

236 ion that resembles a "nutcracker" with two L-terminase protomers projecting from the C-termini of an

237 proteolysis of full-length L-terminase (FL-L-terminase) reveals a central seven-stranded beta-sheet c

238 tomers projecting from the C-termini of an S-terminase ring.

239 ows a fold resembling those of bacteriophage terminases, RNase H, integrases, DNA polymerases, and to

240 stranded DNA viruses by a complex of a small terminase (S-terminase) subunit and a large terminase (L

241 This chimeric terminase's ability to package lambdaDNA is reduced appr

242 Terminase's ATPase consists of a classic nucleotide bind

243 ich gpA residues 46 and 84 are important for terminase's high-affinity ATPase activity.

244 ATPase are highly conserved among >200 large terminase sequences analyzed, these may represent common

245 Sixteen T4-family and numerous phage small terminases show CCMs in the corresponding region of the

246 kaging initiation complex assembled from the terminase small subunit and the packaging region on vira

247 DNA-interacting region on Shigella virus Sf6 terminase small subunit gp1, which occupies extended sur

248 aPI interaction, int (integrase) terS (phage terminase small subunit homologue) and pif (phage interf

249 th phage DNA packaging by blocking the phage terminase small subunit.

250 e complexes to identify or design additional terminase-specific compounds.

251 The small terminase subunit (gp16) is inhibitory for packaging lin

252 headful packaging mechanism and encode small terminase subunit (TerS) homologs that recognize the SaP

253 nto the interaction between the larger pUL56 terminase subunit and the smaller pUL89 subunit.

254 e probed the mechanism of the phage T4 large terminase subunit gp17 by analyzing linear DNAs that are

255 The small terminase subunit has three domains, an N-terminal DNA-b

256 o study the effect of mutations in the large terminase subunit in bacteriophage lambda on packaging m

257 In bacteriophage lambda the catalytic terminase subunit is gpA, which is responsible for matur

258 assemble at the cos site and that the small terminase subunit plays the dominant role in complex ass

259 tein pUL25, the portal protein pUL6, and the terminase subunit pUL33.

260 n, we predicted a classical NLS in the third terminase subunit that is partially conserved among herp

261 que long region 15 (UL15; encoding the large terminase subunit), A374V in UL32 (required for DNA clea

262 only by cos phages, in addition to the large terminase subunit, for cos-site cleavage and melting.

263 eactive residues 46 and 84 of gpA, the large terminase subunit, were studied.

264 y provided by an ATPase present in the large terminase subunit.

265 viruses by a complex of a small terminase (S-terminase) subunit and a large terminase (L-terminase) s

266 -terminase) subunit and a large terminase (L-terminase) subunit, transiently docked at the portal ver

267 ease, both located in the large terminase (L-terminase) subunit.

268 s in elucidating the structure of individual terminase subunits and their domains, little is known ab

269 ell characterized, little is known about the terminase subunits and their molecular mechanism of DNA

270 The five terminase subunits assemble in a toroid that encloses a

271 of a dodecameric portal and small and large terminase subunits assembled at the special head-tail co

272 -driven conformational reorganization of the terminase subunits assembled on viral DNA, which is cent

273 ed that both location and potency of NLSs in terminase subunits evolved more rapidly than the rest of

274 We propose that swapping NLSs among terminase subunits is a regulatory mechanism that allows

275 t that both the pU(L)15- and pU(L)28-bearing terminase subunits mediate docking of the terminase with

276 Previous results indicated that the putative terminase subunits of herpes simplex virus 1 (HSV-1) enc

277 Bioinformatic analysis of large terminase subunits shows that the different functional c

278 al gene assignments included small and large terminase subunits, capsid and tail genes, an N6-DNA ade

279 n the specific DNA interactions of the small terminase subunits, which have support helix-turn-recogn

280 ts lacking functional 80alpha or SaPI1 small terminase subunits.

281 The large terminase (TerL) protein contains both enzymatic activit

282 ts of three components: portal, motor (large terminase; TerL) and regulator (small terminase; TerS).

283 (large terminase; TerL) and regulator (small terminase; TerS).

284 y a virally encoded molecular machine called terminase that consists of two protein components: A DNA

285 ovir is a small-molecule drug targeting HCMV terminase that is currently in phase III clinical trials

286 ith UL15 and UL33 to form a protein complex (terminase) that is presumed to cleave concatemeric DNA i

287 sphohydrolase and endonuclease activities of terminase, the function of gp3 may be to regulate specif

288 etric association with residues 140-162 of S-terminase, the L-terminase binding domain.

289 ture of the portal and its interactions with terminase, the packaging enzyme, are altered, thus signa

290 s a virally encoded molecular machine called terminase to package the viral double-stranded DNA (dsDN

291 y the portal protein, or connector, plus the terminase, which are located at an especial prohead vert

292 erful virally encoded molecular motor called terminase, which comprises large (gp2, 499 residues) and

293 ome by means of a protein complex called the terminase, which is comprised of the HSV-1 UL15, UL28, a

294 Bacteriophage lambda-terminase, which serves as a prototypical genome packagi

295 tiated by the small subunit of the packaging terminase, which specifically binds to the packaging sig

296 The small terminase, which stimulates gp17-ATPase, also stimulates

297 into empty viral procapsids by the phage T4 terminase with high efficiency in vitro.

298 ng terminase subunits mediate docking of the terminase with the portal vertex.

299 petent terminase complex and assembly of the terminase with the portal, in which ring-like protein ol

300 lambda-Terminase with the recognition helix of 21 preferentiall