ライフサイエンスコーパス: terminator

1 emonstrated that one of these functions as a terminator.

2 nes, like NANOG, also have high Ssu72 at the terminator.

3 rth stem-loop functions as a rho-independent terminator.

4 preferred SHM target upstream of the active terminator.

5 art sites and an intervening transcriptional terminator.

6 ynthase 1 and subsequently served as a chain terminator.

7 nator Override (TOV) genes that operate this terminator.

8 serotype-specific repeat unit domain, and a terminator.

9 ze polyubiquitin promoter and a heterologous terminator.

10 at the Rho utilization site of the lambdatR1 terminator.

11 ed ruthenium initiator and a DAN-based chain-terminator.

12 region just upstream of the 3' transcription terminator.

13 ma(S)-dependent promoters but share the same terminator.

14 olor are seen on extended areas close to the terminator.

15 the transposon functions as a transposition terminator.

16 -antiterminator, antiterminator or intrinsic terminator.

17 etic block to invasion of the aptamer by the terminator.

18 reverse transcriptase to act as a DNA chain terminator.

19 ly fewer transcripts are able to bypass this terminator.

20 nd the other downstream of the transcription terminator.

21 function, acting as a non-obligate RNA chain terminator.

22 cture that functions as a factor-independent terminator.

23 hose operating at constitutive Rho-dependent terminators.

24 n the reverse orientation--and 265 synthetic terminators.

25 tly more strong and reliable transcriptional terminators.

26 read-through transcription at Sen1-mediated terminators.

27 th Rho-dependent, as well as Rho-independent terminators.

28 onstraint than promoters and Rho-independent terminators.

29 direction predicted by the disparity of the terminators.

30 only be attributed to the disparity of line terminators.

31 nator during transcription through intrinsic terminators.

32 sh constitutive from regulated Rho-dependent terminators.

33 ased on the study of a small number of model terminators.

34 previous work with photochemically cleavable terminators.

35 fficiently at intrinsic and factor-dependent terminators.

36 sites, coding sequences and transcriptional terminators.

37 incorporate both reversible and irreversible terminators.

38 petition between reversible and irreversible terminators.

39 were after the Rho-independent transcription terminators.

40 te and define a broad class of transcription terminators.

41 ormation of 3'-structural elements acting as terminators.

42 and indirectly via readthrough of suboptimal terminators.

43 d display Pol II pausing downstream from NRD terminators.

44 performance compared to two other reversible terminators, 3'-O-amino-TTP and 3'-O-azidomethyl-TTP.

45 e qPCR SYBR(R)Green technology targeting the terminator 35S pCAMBIA element.

46 Segments from the 5'-UTR, including the terminator 5'-stem-loop and Shine-Dalgarno blocking hair

47 core terminator stem are likely to increase terminator activity.

48 novel two-piece assay that competes the anti-terminator against the aptamer, we directly monitor the

49 ion of 3 previously uncharacterized lncRNAs, TERMINATOR, ALIEN, and PUNISHER, specifically expressed

50 -density small dust particles near the lunar terminators, although later orbital observations yielded

51 polymerase III recognizes and pauses at its terminator, an oligo(dT) tract in non-template DNA, term

52 Deletion of the PapH terminator/anchor resulted in increased OM permeability,

53 of CstF (cleavage stimulatory factor) to the terminator and also the recruitment of the CstF and CPSF

54 it possible to assemble parts with repeated terminator and insulator sequences, and thereby create i

55 sequence redundancies--for example, repeated terminator and insulator sequences--that complicate reco

56 anscription that links the gene promoter and terminator and triggers initiation by RNA pol II.

57 ficity that favors cleavage at promoters and terminators and accounts for some of the correlation bet

58 ranscriptional read-through of selected gene terminators and because it physically interacts with the

59 es), from those that remain refractory (gene terminators and centromeres).

60 tructuring protein (H-NS) with Rho-dependent terminators and genetic interactions between hns and rho

61 ility in the structure of factor-independent terminators and identifies a mechanism for generation of

62 xtent of sequence diversity among functional terminators and the extent of mechanistic variation as a

63 binding dual-hairpin structures that overlap terminators and thus prevent transcription termination.

64 whose flanking regions including promoters, terminators and untranslated sequences could drive stabl

65 rminator clones compared with an inactivated terminator, and this region showed more single-stranded

66 ossovers increased toward gene promoters and terminators, and hot spots were associated with active c

67 Pcf11 localizes downstream from Nrd1 on NRD terminators, and its recruitment depends on Nrd1.

68 acity, requirement of multiple promoters and terminators, and variable transgene expression levels.

69 The refolding kinetics of a bistable terminator antiterminator segment involved in the gene r

70 Most terminators are designed with 3'-O-blocking groups but a

71 Promoters or terminators are excluded from functional TUs by read-thr

72 Promoters and terminators are generally located between genes and toge

73 Two types of bacterial transcriptional terminators are known to control gene expression.

74 Rho-dependent terminators are sites of dissociation mediated by an RNA

75 pletion strain, we show that weak suboptimal terminators are the principle NusA substrates.

76 gh a few intrinsic prokaryotic transcription terminators are used routinely, termination efficiencies

77 53/37 and C11 to slow elongation and prevent terminator arrest.

78 The results reveal the RNAP III terminator as an information-rich control element.

79 of the entire Rho-independent transcription terminator associated with the proK, proL and proM prima

80 TUs each have their own promoters; (iii) the terminators associated with the 3' ends of TUCs tend to

81 ct regulatory elements such as promoters and terminators associated with the novel expressed regions

82 ator, which allows formation of an intrinsic terminator (attenuator).

83 Switching of this terminator between active and inactive states dictates t

84 ay variability, and were seen at the morning terminator but not at the evening limb, which indicates

85 scripts ended at intrinsic (rho-independent) terminators, but most of the other transcripts seemed to

86 GT modules are separated from the GT99 chain terminator by a coiled-coil structure that forms a molec

87 to promoter sequences or as a transcription terminator by blocking the running RNAP.

88 Nascent pre-tRNAs released from a terminator by C37 mutants have shorter 3'-oligo(U) tract

89 t base-selective incorporation of reversible terminators by DNA polymerases.

90 ecially important for understanding how such terminators can be regulated in response to specific sig

91 Readthrough of NusA-dependent terminators caused misregulation of genes involved in es

92 One of the two major terminator classes described so far is the Co-Transcript

93 ly(A) site were almost doubled in the active terminator clones compared with an inactivated terminato

94 on units (TUs), using different promoter and terminator combinations, that underlie state-switching.

95 Intrinsic transcription terminators consist of an RNA hairpin followed by a U-ri

96 wed that plants expressing gfp with the rrnB terminator contained 4 times more gfp transcripts than p

97 These findings prove that terminators contribute to stereo matching, and constrain

98 We discovered that the terminator deletion mutant is highly resistant to neutro

99 Unlike Rho-dependent terminators described previously, where termination occu

100 at might be responsible for the detection of terminator disparity.

101 Intrinsic terminators dissociate transcription complexes without t

102 A later study demonstrated that if the terminator DNA is partially unwound, the resulting melte

103 rial replication intermediates, an RNA chain terminator does not.

104 ough of transcription at the Rho-independent terminators downstream of araD and araE, leading to sign

105 Finally, terminators drive vergence even when the aperture is def

106 that the trp attenuator is a weak intrinsic terminator due to low GC content of the hairpin stem and

107 on, presumably through its action as a chain terminator during DNA replication.

108 ve copy number information from standard dye-terminator electropherograms has been little explored, y

109 ents formation of an intrinsic transcription terminator element located within the intercistronic reg

110 s within the human beta-globin CoTC-mediated terminator element play a critical role in Pol II termin

111 th transcription termination at the oligo(T) terminator element, which forms a 3' oligo(U) tract on t

112 uired for gene loops that juxtapose promoter-terminator elements in a transcription-dependent manner.

113 n RNA processing signals as well as specific terminator elements located downstream of the poly(A) si

114 ithin seemingly random sequences, are strong terminator elements, and bioinformatics analysis confirm

115 -loops are particularly enriched over G-rich terminator elements.

116 either at the pA site or in transcripts from terminator elements.

117 Deletion of hasS or of the terminator eliminates CovR-binding sequences, relieving

118 By excluding terminators encoding such context-confounding elements,

119 id aggregation of the eukaryotic translation terminator eRF3/Sup35p, the [PSI (+)] prion, empowers ye

120 e, we demonstrate that these 3'-OH unblocked terminators exhibit superior enzymatic performance compa

121 condary structures that might interfere with terminator folding kinetics or impact termination activi

122 ific anti-herpetic drug, acts as a DNA chain terminator for several human herpesviruses (HHVs), inclu

123 lopment of a complete set of four reversible terminators for application in NGS technologies.

124 nylation after Rho-independent transcription terminators for both mono- and polycistronic transcripts

125 2'-C-Me-DAPN-TP and 2'-C-Me-GTP were chain terminators for genotype 1b HCV-pol, and single nucleoti

126 tor I/II complexes function as transcription terminators for human snRNA genes with little, if any, r

127 model of phase variation based on intrinsic terminator formation in the OFF transcript.

128 conformation that is more accessible to anti-terminator formation.

129 in the context of aptamer collapse and anti-terminator formation.

130 P are utilized in the processing of proL The terminator fragment derived from the endonucleolytic cle

131 lear which enzymes degrade the proK and proM terminator fragments.

132 e) removes the Rho-independent transcription terminator from the leuX transcript without requiring th

133 t removing the Rho-independent transcription terminators from the primary valU and lysT transcripts.

134 on of anti-antiterminator, antiterminator or terminator function by competitor oligonucleotides in vi

135 tion of bacterial sequence features, such as terminators, functional sRNAs, and operons.

136 rovided with a Rho-independent transcription terminator, gene regulation was no longer PNPase-depende

137 n of transcriptional elements (promoters and terminators) generates new operons, restores the coordin

138 into the trigger helices and contacting the terminator hairpin after invasion of the hairpin in the

139 ing, an equivalent role for the disparity of terminators has not been established.

140 stabilizes the Rho-RNA interactions at many terminators having suboptimal rut sites, thus making Rho

141 dicted to function as archaeal transcription terminators, identically positioned between a constituti

142 ercoming an otherwise stable transcriptional terminator if the bound tRNA is uncharged.

143 ts as an alternative substrate and RNA-chain terminator in primer-extension assays using a surrogate

144 t was reduced by a potential transcriptional terminator in promoter region 298 to 397 with a DeltaG =

145 o effect on antisense or sense Rho-dependent terminators in vivo.

146 yces pombe that cause pol III to readthrough terminators in vivo.

147 have measured the strengths of a library of terminators, including 227 that are annotated in Escheri

148 Template-directed dye-terminator incorporation assay with fluorescence polariz

149 e substitutions that cause disruption of the terminator interfere with the regulatory function of UTR

150 uired for SHM, we knocked-in a transcription terminator into an Ig gene variable region in DT40 chick

151 ongation of prgQ transcripts past a putative terminator (IRS1) may be controlled by the interaction o

152 This terminator is based on 5-hydroxymethyl-2'-deoxyuridine t

153 The RNA polymerase III transcription terminator is flexibly accommodated within the transcrip

154 ated immediately upstream from the intrinsic terminator is necessary for subsequent degradation to oc

155 shed association of TFIIB E62K with the PMA1 terminator is restored by the Ssl2 H508R suppressor.

156 The function of this terminator is strongly attenuated by upstream mcc sequen

157 egions suggests that nucleosome depletion at terminators is not simply associated with passage of pol

158 In addition, nucleosome occupancy at terminators is strongly affected by growth conditions, i

159 at promoter regions, nucleosome occupancy at terminators is strongly correlated with the orientation

160 sm of action of this hexameric transcription terminator, its regulation by different cis and trans fa

161 presence of a rho-independent transcription terminator just 28 bp upstream of the main translation s

162 acrophages and also predicts that rNTP chain terminators lacking a 3'-OH should inhibit HIV-1 reverse

163 h precise mutations in promoters, genes, and terminators, leading to altered carotenoid levels.

164 ase terminates transcription at an intrinsic terminator located in the intergenic region between the

165 ctinomycetemcomitans strain and identified a terminator located in the promoter region extending from

166 y expressed, is regulated by a Rho-dependent terminator located within its 5' leader region.

167 ages is unique and that ribonucleoside chain terminators may be a new class of anti-HIV-1 agents spec

168 A)) that conforms to the bacterial intrinsic terminator motif reduced TK1761 expression approximately

169 ily, the human mitochondrial transcriptional terminator MTERF1, bound to dsDNA containing the termina

170 that the sequences at the distal tip of the terminator not directly involved in alternative secondar

171 disrupt interactions with transcription anti-terminator, NusA.

172 stages of axial elongation, not solely as a terminator of axial growth.

173 tract 5'-rUUUUUAU-3' from the tR2 intrinsic terminator of bacteriophage lambda.

174 osphate (EFdA-MP) acted mainly as a de facto terminator of further RT-catalyzed DNA synthesis because

175 -containing protein 2 (PDLIM2), an essential terminator of NF-kappaB activation, is repressed in both

176 Here, we report that PDLIM2, an essential terminator of NF-kappaB activation, is repressed in vari

177 Consistent with a structural role as terminators of secondary structure elements, substitutio

178 g hydrogen-bonding capabilities act as chain terminators of translesion DNA replication while analogs

179 t terminators, substantially more often than terminators of TUs that end inside a TUC; and (iv) the f

180 The availability of many terminators of varying strength, as well as an understan

181 the biologically active forms, act as chain terminators of viral DNA synthesis.

182 context of motion detection, the endings (or terminators) of 1-D features can be detected as 2-D feat

183 >4 mM) shift the equilibrium toward the anti-terminator on-state even in the presence of SAM.

184 Further, it contains transcriptional terminators on both sides of the cloning site to minimiz

185 larity (i.e. aptamer 'off-state' versus anti-terminator 'on-state').

186 The current study describes Terminator Operon Reporter (TOR), a new gene assembly te

187 genes through the tRNA-binding formation of terminator or antiterminator structures.

188 norbornene octyl ester with a CA-based chain-terminator or by the reaction of poly(ethylene oxide) wi

189 on or deletion of DNA encoding transcription terminators or a promoter modulates transcription rates.

190 either at specific DNA sequences, called the terminators, or by a nascent RNA-dependent helicase, Rho

191 The intrinsic trp leader terminator overlaps the hairpin-dependent U144 pause sit

192 ad-through assay, we identified trans-acting Terminator Override (TOV) genes that operate this termin

193 We found strong co-variation support for the terminator, P1, and anti-terminator stems in the purine

194 ssembly to construct repeating promoter-gene-terminator parts, we systematically varied gene expressi

195 cted a systematic mutational analysis of the terminator/pause region.

196 The Escherichia coli replication terminator protein (Tus) binds to Ter sequences to block

197 modulated by asymmetric contacts between the terminator protein and its cognate DNA sequence.

198 ns between the replication fork helicase and terminator protein are the primary mechanism for polar f

199 The replication terminator protein Fob1 of Saccharomyces cerevisiae is m

200 Here, we show that the replication terminator protein Fob1 of Saccharomyces cerevisiae prom

201 : elophase exit) complex and the replication terminator protein Fob1.

202 In E. coli and Bacillus subtilis, the bound terminator protein makes protein-protein contacts with t

203 propose that XA10 is an inducible, intrinsic terminator protein that triggers programmed cell death b

204 A hexamer of the bacteriophage T4 tail terminator protein, gp15, attaches to the top of the pha

205 sulting melted DNA could bind tightly to the terminator protein, suggesting a mechanism for polar arr

206 is process requires the binding of the polar terminator protein, Tus, to specific DNA sequences calle

207 However, recent evidence suggests that the terminator protein-DNA contacts are not sufficient for p

208 nal communications between RNA pol I and the terminator protein.

209 terference by bi-directional transcriptional terminators proven to be highly efficient in in vitro tr

210 9, n = 31) better than models trained on all terminators (r = 0.67, n = 54).

211 While a DNA chain terminator rapidly blocks the labeling of mitochondrial

212 Using a sensitive terminator read-through assay, we identified trans-actin

213 3 and C37 and isolated many C37 mutants with terminator readthrough but no comparable C53 mutants.

214 Mutant RNA confirmed the presence of terminator readthrough transcripts.

215 No such terminator readthrough was observed in the mutant at the

216 ctivities: RNA oligo(U) 3'-end cleavage, and terminator readthrough.

217 of water as the chain transfer and/or chain terminator reagent, which is added at the end of the sal

218 ategy of creating 3'-OH unblocked reversible terminator reagents that, upon photochemical cleavage, t

219 tinct activities, that differentially affect terminator recognition and RNA 3' cleavage.

220 The terminator reduced mutations downstream of the poly(A) s

221 last rbcL, psbA, petD and rpoA genes and the terminator region of the Escherichia coli rrnB operon we

222 ex and facilitating their recruitment to the terminator region.

223 bryonic stem cells, with a minor peak in the terminator region.

224 nce of such sequences in 70% of the putative terminator regions (PTRs) genome-wide.

225 TFIIB also cross-links to terminator regions and is required for gene loops that j

226 However, the distinct behavior of terminator regions and their corresponding coding region

227 ongly depleted of nucleosomes, but find that terminator regions are much less depleted than expected.

228 ase (MNase) suggests that yeast promoter and terminator regions are very depleted of nucleosomes, pre

229 IB cross-linked to both the promoter and the terminator regions during the transcriptionally activate

230 d as the interaction of the promoter and the terminator regions of a gene during transcription, requi

231 onserved genic hotspots such as promoter and terminator regions of poly(A)-dependent genes.

232 DNA loops that juxtapose the promoter and terminator regions of RNA polymerase II-transcribed gene

233 D18 associates with the promoter, coding and terminator regions of target genes suggesting its functi

234 ation, Ess1 associates with the promoter and terminator regions of the PMA1 and PHO5 genes.

235 ce (UAS) and promoter elements, promoter and terminator regions, and regulatory and coding regions (g

236 l2, like TFIIB, associates with promoter and terminator regions, and the diminished association of TF

237 physical interaction of the promoter and the terminator regions.

238 RNase E, G and Z are not involved in terminator removal.

239 y removing the Rho-independent transcription terminator represents a previously unidentified function

240 induced by the formation of transcriptional terminators represents a prime example for the coupling

241 ound that a bacterial protein, transcription terminator Rho of Clostridium botulinum (Cb-Rho), could

242 eporters, repressors, activators, promoters, terminators, ribosome binding sites, signaling devices,

243 Bacterial Rho-independent terminators (RITs) are important genomic landmarks invol

244 tion mechanisms and the relationship between terminator sequence and steps in the termination mechani

245 A DNA replication terminator sequence blocks an approaching replication fo

246 We used these data to determine how the terminator sequence contributes to its strength.

247 poly(A) (pA) signal and, often, a downstream terminator sequence.

248 In yeast, terminator sequences are present in rDNA non-transcribed

249 t of the HasS variations were located in the terminator sequences, suggesting that this region is und

250 iboswitch by extending alignments to include terminator sequences.

251 monly used Hsp70A-RBCS2 (AR) hybrid promoter/terminator sequences.

252 Sanger dideoxy terminator sequencing allows for rapid development and i

253 tion followed by Illumina NextSeq reversible terminator sequencing with DNA selection by amplificatio

254 ulting systematically measured collection of terminators should improve the engineering of synthetic

255 Lightning Terminators show maximum incorporation rates (k(pol)) th

256 hybrids from the tR2 intrinsic transcription terminator site of phage lambda.

257 ltifunctional proteins that bind to specific terminator sites (Ter) and cause polar termination of tr

258 nd that structures extending beyond the core terminator stem are likely to increase terminator activi

259 eotide CAUAGC to form either a pseudoknot or terminator stem.

260 ion support for the terminator, P1, and anti-terminator stems in the purine riboswitch by extending a

261 II) causes increased nucleosome occupancy at terminators, strongly suggesting a transcription-based m

262 a specific UTR1 conformation that favors the terminator structure in Mn(2+)-replete condition.

263 binds to trp operon leader RNA, generating a terminator structure that promotes transcription termina

264 required for the full formation of the anti-terminator structure, and that higher concentrations of

265 RNA fragments that contain the transcription terminator structure.

266 e 3' ends of TUCs tend to be Rho-independent terminators, substantially more often than terminators o

267 e-subunit complexes except for the chaperone-terminator subunit (PapDH) and has a catalytic role in d

268 NusA-dependent terminators tend to have weak hairpins and/or distal U-t

269 ptic (kissing) interactions between pairs of terminator (Ter) sites that initiated recombination in r

270 917 insertions accumulate around the central terminators, terI and terII, in wild-type cells with or

271 mall RNA, HasS, an intrinsic transcriptional terminator that inefficiently terminates HasS, permittin

272 hese errors are monitored by a transcription terminator that is placed between the synthetic gene and

273 upstream of the TH or point mutations in the terminator that preserve TH stability affect termination

274 led a collection of 61 natural and synthetic terminators that collectively encode termination efficie

275 ence elements such as standard promoters and terminators that interfere with homologous recombination

276 arts (promoters, ribosome binding sites, and terminators) that are functionally separated by spacer p

277 xtremely high-altitude plumes at the Martian terminator (the day-night boundary) at 200 to 250 kilome

278 coassociates with both the promoter and the terminator, the inactivation of Ssu72 leads to increased

279 p structure of rho-independent transcription terminators, thus avoiding intact genes.

280 oeae functions as an intrinsic transcription terminator to generate non-stop mRNA.

281 ) transporter, which forms a Rho-independent terminator to implement transcription termination with a

282 ression of the mitochondrial transcriptional terminator transcription factor 3 (MTERF3).

283 /IL-24, we created a tropism-modified cancer terminator virus (Ad.5/3-CTV), which selectively replica

284 EG-E1A-mda-7; also called Ad.5/3-CTV (cancer terminator virus)].

285 We found that the human beta-globin terminator was an efficient inhibitor of downstream tran

286 The presence of TFIIB on the terminator was dependent on the Rna15 component of CF1 3

287 on downstream of the predicted transcription terminator was dose dependent and specific to c-di-GMP b

288 Moreover, a subclass of weak non-canonical terminators was identified that completely depend on Nus

289 sampling on purine riboswitch sequences with terminators we found that these sequences appear to have

290 V) was identified as an efficient reversible terminator, whereby, sequencing feasibility was demonstr

291 t, a mutation within the rho transcriptional terminator, which defines an alternative adaptive pathwa

292 '-OH unblocked nucleotides, called Lightning Terminators, which have a terminating 2-nitrobenzyl moie

293 ent that GraL arrays arise from an intrinsic terminator with an 11 bp stem followed by an AU(7)GCU(2)

294 n interacted with the 5' splice site and the terminator with the 3' splice site.

295 describe a novel 3'-OH unblocked reversible terminator with the potential to improve accuracy and re

296 %) of both antisense and sense Rho-dependent terminators with lower C/G ratio sequences.

297 We synthesized reversible terminators with tethered inhibitors for next-generation

298 I subunits or insertion of an ectopic Pol I terminator within the adjacent rDNA gene.

299 es, where each gene has its own promoter and terminator, within the same T-DNA.

300 Within this library we found 39 strong terminators, yielding >50-fold reduction in downstream e