ライフサイエンスコーパス: terpene synthase

1 esyl diphosphate synthase and an appropriate terpene synthase.

2 ranscripts for eight genes encoding distinct terpene synthases.

3 shows some similarity to sequences of other terpene synthases.

4 es these enzymes in a noncanonical family of terpene synthases.

5 al products by providing a homologous set of terpene synthases.

6 lly related to the family of proteins termed terpene synthases.

7 ases and only distantly so to microbial-type terpene synthases.

8 scades and promiscuity mechanisms of class I terpene synthases.

9 acilitate the functional assignment of novel terpene synthases.

10 rase mechanism of UbiX resembles that of the terpene synthases.

11 istent with proposed evolutionary origins of terpene synthases.

12 o catalytic activity of 10 additional tomato terpene synthases.

13 r helices rather than six found in all other terpene synthases.

14 -component protein located downstream of the terpene synthases.

15 hetic properties in phylogenetically related terpene synthases.

16 ery and mechanistic analysis of golden larch terpene synthase 8 (PxaTPS8), an unusual diterpene synth

17 site moonlighting on it and the first time a terpene synthase active site is found moonlighting on an

18 y structure indicate the presence of a novel terpene synthase active site that is moonlighting on the

19 erpenes were correlated with total levels of terpene synthase activities, and negatively correlated w

20 lishes that this barrel is essential for the terpene synthase activity of CYP170A1 but not for the mo

21 3) whose members have a characteristic alpha terpene synthase alpha-helical fold.

22 well as structural comparisons with diverse terpene synthases and cyclases which clearly separate th

23 families of conifer defense metabolism, the terpene synthases and cytochrome P450s.

24 tandardized method to facilitate analysis of terpene synthases and diverse mutant enzyme libraries by

25 s, not related to previously described plant terpene synthases and only distantly so to microbial-typ

26 nscript levels of a gene encoding a putative terpene synthase are induced in mechanically- or insect-

27 Terpene synthases are a mechanistically intriguing famil

28 ween two distinct yet evolutionarily related terpene synthases based on the systematic identification

29 isoprene depends on whether or not it has a terpene synthase capable of using dimethylallyl diphosph

30 "Head-to-head" terpene synthases catalyze the first committed steps in

31 ugh advances in cereal genome annotation and terpene synthase characterization that likewise enable d

32 e gene level by RNA-blot hybridization using terpene synthase class-directed DNA probes.

33 thematical model in order to construct novel terpene synthases, each catalysing the synthesis of one

34 The terpene synthase encoded by the sav76 gene of Streptomyc

35 Here we report that only two terpene synthases, encoded by the florally expressed gen

36 s are produced in our system by changing the terpene synthase enzyme.

37 rgy surfaces, but also for the mechanisms of terpene synthase enzymes and their evolution.

38 ortance of inherent substrate reactivity for terpene synthase enzymes is discussed, with a focus on r

39 ases make the active site smaller than other terpene synthase enzymes, possibly conferring specificit

40 ied out with OsCPSsyn revealed that class II terpene synthases exhibit a sequence conservation patter

41 Mutations of residues outside of the alpha terpene synthase fold are important for acquisition of F

42 ach subunit adopts the alpha-helical class I terpene synthase fold with the active site in the "open"

43 The biochemical function of all four terpene synthases found in the S. avermtilis genome have

44 this gymnosperm do not very closely resemble terpene synthases from angiosperm species (52-56% simila

45 Interestingly, distantly related terpene synthases from fungi to plants all contain an in

46 dicates that other such class I and class II terpene synthase gene clusters may similarly catalyze co

47 T1, SlCPT2 and SlCPT6) are closely linked to terpene synthase gene clusters.

48 expressed exclusively in the flowers and one terpene synthase gene expressed almost exclusively in th

49 Phylogenetic analysis of the terpene synthase gene family indicated that isoprene syn

50 uction of any terpenoid compound for which a terpene synthase gene is available.

51 The maize (Zea mays subsp. mays) terpene synthase gene stc1 is part of that indirect defe

52 mRNA for a terpene synthase gene, phellandrene synthase 1 (PHS1), w

53 Class I terpene synthases generate the structural core of bioact

54 esources, we identified seven V. officinalis terpene synthase genes (VoTPSs), two that were functiona

55 to eight previously characterized angiosperm terpene synthase genes and to six putative terpene synth

56 , the phylogenetic analysis revealed the two terpene synthase genes as primitive genes that might hav

57 Two terpene synthase genes expressed exclusively in the flow

58 a HMMER search tool to identify 17 putative terpene synthase genes from M. polymorpha transcriptomes

59 on of P450 genes with their adjacent located terpene synthase genes in E. coli demonstrates that the

60 Of the 32 terpene synthase genes in the Arabidopsis genome, 20 wer

61 r natural products biosynthesis derived from terpene synthase genes involved in primary metabolism by

62 Three distinct classes of terpene synthase genes were discerned, from which assume

63 New terpene synthase genes were isolated from flowers of Nic

64 late E7406B, we were able to identify 11 new terpene synthase genes.

65 ra of rhizobia were found to have homologous terpene synthase genes.

66 m terpene synthase genes and to six putative terpene synthase genomic sequences from Arabidopsis thal

67 n of (S)-beta-citronellol commences with the terpene synthase GES1 catalyzing the irreversible conver

68 in many natural water supplies; however, no terpene synthases have been characterized from these org

69 Although these two terpene synthases have very similar catalytic properties

70 rly separate the terpene cyclases from other terpene synthases having highly alpha-helical structures

71 The second terpene synthase in N. punctiforme (NP2) is homologous t

72 c 'insertional' sequence element in class II terpene synthases, indicating that this region is import

73 wledge, this is the first documentation of a terpene synthase involved in the synthesis of a linear t

74 ic catalysis of abietadiene cyclization, and terpene synthases more broadly.

75 thase NS1) and Nostoc punctiforme PCC 73102 (terpene synthases NP1 and NP2).

76 Terpene synthases NP1 and NS1 are part of an apparent mi

77 es identified in Nostoc sp. strain PCC 7120 (terpene synthase NS1) and Nostoc punctiforme PCC 73102 (

78 and analysis of six genomic clones encoding terpene synthases of conifers, [(-)-pinene (C(10)), (-)-

79 Terpene synthases often catalyze complex cyclization rea

80 Terpene synthases often catalyze complex reactions invol

81 PS1ent in maize (Zea mays) than its class II terpene synthase paralogs involved in rice secondary met

82 high degree of structural relatedness among terpene synthases, previous studies suggest that no clea

83 The class II terpene synthase producing syn-copalyl diphosphate from

84 Substrate promiscuity of terpene synthases provides organism access to novel chem

85 Promiscuity of terpene synthases results in the enormous diversity of t

86 rgent evolution, mutational analysis of this terpene synthase revealed an active site asparagine crit

87 Accordingly, how terpene synthases specify product outcome has drawn a gr

88 me implications regarding the means by which terpene synthases specify product outcome.

89 sented for the evolutionary history of plant terpene synthases suggests that this superfamily of gene

90 urprising finding of an atypical class I (di)terpene synthase that acts on CPP to produce the abietan

91 natural products is catalyzed by the class I terpene synthase that converts syn-copalyl diphosphate t

92 Thus, the class II terpene synthase that converts the universal diterpenoid

93 ene synthase (ZIS) gene encoding a cytosolic terpene synthase that has been shown to possess both ses

94 challenges for the functional assignment of terpene synthases that construct the carbon skeletons of

95 (E)-beta-farnesene synthase (BFS), a pair of terpene synthases that produce cyclic or linear terpenes

96 lly the alpha, alphabeta, and alphabetagamma terpene synthases that produce plant terpenes, with many

97 ata were used to identify eight putative (di)terpene synthases that were then characterized for their

98 into the evolutionary relationship of fungal terpene synthases to those in plants and bacteria and fu

99 terpenes called rhizathalenes by the class I terpene synthase (TPS) 08 in roots of Arabidopsis thalia

100 s could be assigned to previously identified terpene synthase (TPS) activities that included members

101 Four of these putative trans-IDSs exhibited terpene synthase (TPS) activity when heterologously expr

102 trans-prenyltransferase (PT) and N-terminal terpene synthase (TPS) domains.

103 hase (GLS) belonging to the e/f clade of the terpene synthase (TPS) family and two Fabaceae GLSs that

104 4 (At1g61680), define a new subfamily of the terpene synthase (TPS) family designated the Tps-g group

105 Three cDNAs corresponding to terpene synthase (TPS) genes expressed in young leaves w

106 t induction of expression of seven of the 11 terpene synthase (TPS) genes identified through the micr

107 ed tomato (Solanum lycopersicum) contains 44 terpene synthase (TPS) genes, including 29 that are func

108 apple (Malus domestica) contains 55 putative terpene synthase (TPS) genes, of which only 10 are predi

109 sesquiterpene biosynthesis in sorghum, seven terpene synthase (TPS) genes, SbTPS1 through SbTPS7, wer

110 e show that all four selected genes, the two terpene synthases (TPS10 and TPS14) and the two cytochro

111 ism in the promoter of the gene encoding the terpene synthase TPS2 with this QTL Biochemical characte

112 Terpene synthases (TPSs) are pivotal enzymes for the bio

113 has been well studied, with most if not all terpene synthases (TPSs) being identified.

114 The extensive family of plant terpene synthases (TPSs) generally has a bi-domain struc

115 To identify terpene synthases (TPSs) involved in the production of t

116 In plants, a family of terpene synthases (TPSs) is responsible for the synthesi

117 lycopersicum; Solanaceae) contains genes for terpene synthases (TPSs) that specify the synthesis of m

118 he pivotal enzymes for terpene biosynthesis, terpene synthases (TPSs), had been described only in pla

119 contains over 30 genes potentially encoding terpene synthases (TPSs).

120 of a large gene family (with 20 members) for terpene synthases (TPSs).

121 -dependent cyclization cascades catalyzed by terpene synthases (TSs).

122 nd phase, and exon size) of these gymnosperm terpene synthases was compared to eight previously chara

123 and terpenoid compounds, including putative terpene synthases, were first identified by mining publi

124 taxadiene synthase (TXS), the model class I terpene synthase, which simulates the initial catalytic

125 Of particular interest are terpene synthases, which often mediate the committed ste

126 our knowledge, this is the first identified terpene synthase with this particular substrate stereose