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1 e exposure of live cells to the dyes without the necessity of washing.
2 kov models from noisy time sequences without the necessity of assuming a model architecture, thus all
3                                              The necessity of conducting a functional behavioural ass
4  in particle refractivity indices, delineate the necessity of creating a calibration curve to adjust
5          The differences might be related to the necessity of developing a free-living tadpole quickl
6 based models of nectar feeding and highlight the necessity of developing a new biophysical model for
7  previously, performance has been limited by the necessity of devoting a large fraction of the materi
8               The slow folding may be due to the necessity of forming a buried polar interaction in t
9 icient patients if it were possible to avoid the necessity of generating a separate line for each pat
10                     In a future perspective, the necessity of having a clear working definition of bi
11 tions (62.8+/-1.6 to 117.4+/-3.0 mug/kg) and the necessity of implementing a preconcentration method.
12            The present study also emphasizes the necessity of including a non-treated, baseline group
13 ical significance of CK isoenzyme shifts, 2) the necessity of maintaining a high CK reaction rate, an
14 have high quantum yields (up to 65%) without the necessity of overcoating a shell.
15 cientists, since it is expected to eliminate the necessity of replenishing a damaged material.
16 ule inhibitors has proved difficult owing to the necessity of targeting a protein-protein interaction
17 ral enemies on herbivorous insects, and thus the necessity of using a tri-trophic approach when study
18 iple inflammatory disorders, and underscores the necessity of using a wide range of investigative tec
19                   This study also highlights the necessity of having access to hospitalization to obs
20                                              The necessity of ascertaining actual facility capability
21                     The use of oligos avoids the necessity of preparing adaptors and the problems ass
22 ecies unrelated to B. anthracis alerts us to the necessity of identifying additional sequences that w
23 rative clinical staging of renal tumors, and the necessity of completing all the components of the ra
24 argets activated by NF-kappa B signaling and the necessity of differentiating among these genes for t
25 cyclophane 28 brought additional support for the necessity of having an E-hydrogen atom at the termin
26                                              The necessity of having an electron-withdrawing group al
27                                              The necessity of introducing an unexpected concept, name
28 l) flanked by A-rich sequences, underscoring the necessity of using an accurate tool for pA mapping.
29                         Also, it illustrates the necessity of using and developing models that handle
30            Discussion: This study highlights the necessity of validating and deploying vector control
31                                              The necessity of detecting antibodies to viral envelope
32  surface of HCV-specific T cells, supporting the necessity of ongoing antigenic stimulation of T cell
33 renes in the presence of TBAF in DMF without the necessity of adding any ligands or additives to give
34 cer etiology and progression become defined, the necessity of looking at multiple genes and their int
35                   Our results thus highlight the necessity of including backbone flexibility to corre
36  vivo or to produce immune responses without the necessity of cloning, bacterial propagation, or prot
37  the fierce public and political disputes on the necessity of controlling badgers to limit the spread
38          Specifically, these results suggest the necessity of optimizing biocide application at the l
39                       These results indicate the necessity of integrating both gene association and g
40  providing an internal mass standard without the necessity of mixing calibrant and analyte.
41 or quantification of the trace gases without the necessity of performing calibrations with authentic
42               This two-step procedure avoids the necessity of transporting catalysts through the aque
43 cell sensitivity to 5FU, clearly demonstrate the necessity of exercising caution in considering combi
44 andidate clones for further analysis without the necessity of screening cDNA libraries.
45                       This review highlights the necessity of smoking cessation as a cornerstone of c
46  characteristics of UHI effect and emphasize the necessity of considering city- and time-specific FP
47  disease burden, lengthening the time before the necessity of undertaking combination therapy with ot
48 r joint application, these results highlight the necessity of establishing combinatorial analytical a
49 at mimic discontinuous protein epitopes, and the necessity of combining complementary experimental ap
50 inbred genome as a source of ESTs eliminates the necessity of computing covariance on each polymorphi
51               These findings also underscore the necessity of screening current and future drugs at h
52                    These observations reveal the necessity of building disease-specific classifiers t
53 e kinetics of one-dimensional motion without the necessity of measuring DNA or ATP hydrolysis.
54                        They also alert us to the necessity of assessing drug effects at different lev
55                       Our findings highlight the necessity of considering drug exposure history when
56                      These results highlight the necessity of understanding electronic structure chan
57                             This study shows the necessity of considering epistatic effects in soybea
58 important because they minimize or eliminate the necessity of using expensive and complicated instrum
59 and beta3 assemble with alpha4 and beta2 and the necessity of avoiding formation of potentially toxic
60 ndistinguishable from AD, and they highlight the necessity of screening frontotemporal dementia genes
61 tinue to influence society's views regarding the necessity of testing germ cells for mutations to pre
62 azoles, amines, and carboxylic acids without the necessity of protecting groups.
63                     These findings highlight the necessity of developing guidelines and policies that
64                                              The necessity of examining human ES cells in culture, co
65 ers as heterodimers, it may also result from the necessity of altering independent biochemical pathwa
66 involvement of HSV1 and cytomegalovirus (and the necessity of taking into account any marked differen
67                     These findings emphasize the necessity of taking into account the variability of
68 , requiring substantial user interaction and the necessity of building intuition, all of which are ti
69 , switch status of an OMP gene), emphasizing the necessity of characterizing isolates before and afte
70 cular organization in device performance and the necessity of optimizing it to take full advantage of
71 several regions of the world has resulted in the necessity of performing keratoplasty with imported d
72 ally whether tunneling is important, without the necessity of measuring kT.
73 epidemiological data and modelling underline the necessity of addressing latent tuberculosis infectio
74 with DURC; and how to balance this risk with the necessity of fostering life sciences research for pu
75  system can be scaled as needed and obviates the necessity of using limited blood products obtained f
76 gs of human germline variation may challenge the necessity of sequencing matched tumor-normal pairs.
77 g messages electronically increases, so does the necessity of conveying messages more efficiently.
78 ons of the bronchial tree completely removes the necessity of examining multiple sections and perform
79                             Our data support the necessity of studying multiple clones when using iPS
80          They have also shed bright light on the necessity of designing multitasking ligands, display
81           Obesity is increasing, yet despite the necessity of maintaining muscle mass and function wi
82 les imposed by economic forces and driven by the necessity of finding new sources of energy, particul
83                  Meanwhile, the tests showed the necessity of using nucleic acid confirmatory tests t
84  protein in a given biological fluid without the necessity of determining or guessing which other pro
85                     These observations imply the necessity of subtyping patients considered for pepti
86                         Our understanding of the necessity of considering peripheral properties when
87          This study empirically demonstrates the necessity of examining phenotype-determining genomic
88  the treatment of bite victims, highlighting the necessity of using pooled venoms as a substrate for
89  the advantages of cumulative research; (ii) the necessity of aligning projects with research priorit
90 s on the protein kinase benchmark underscore the necessity of incorporating protein flexibility in bo
91 f PsbO in the SRM analysis, which highlights the necessity of validating protein changes by more than
92 n that enables analysis of the data, without the necessity of determining quenching parameters for di
93 our immune defenses must be balanced against the necessity of mounting rapid immune responses to an e
94 ch, and provides testimony to Congress about the necessity of furthering research activities related
95 ed for complex motion patterns and advocates the necessity of combining retinal and extraretinal sign
96  onset latencies is caused experimentally by the necessity of using reward reinforcement to motivate
97 lan that places strong and clear emphasis on the necessity of improving RI to achieve and sustain glo
98  from the literature and further demonstrate the necessity of incorporating RNA tertiary structural i
99 xicity pattern for plants exposed to Ag-NPs, the necessity of monitoring several markers to accuratel
100 ral network of single-step mutations without the necessity of invoking simultaneous mutations, low-fi
101                         Our results point to the necessity of developing site-specific drug carriers
102                   Thus, this study indicates the necessity of inhibiting SOCS1, an antigen presentati
103 ction, nevertheless, is controversial due to the necessity of using stabilizing chemicals to help ret
104 n early and evolving MS lesions, and suggest the necessity of ongoing studies of the mechanisms under
105 e earthquake cause, our research underscores the necessity of monitoring subsurface wastewater format
106 g and can guide clinical decisions regarding the necessity of obtaining such imaging.
107                           This method avoids the necessity of finding suitable primers for long PCRs
108                            We also highlight the necessity of using symmetric datasets for finding so
109                            Thus we find that the necessity of allowing the tetracationic ring to beco
110 es between young and old samples, suggesting the necessity of considering the age effects for persona
111 windows over alignment columns, illustrating the necessity of considering the local environment of po
112 ngaged in a variety of tasks; and (3) reveal the necessity of coupling the neuronal networks to chemi
113 ty of the Ad5 receptor in vivo and emphasize the necessity of demonstrating the nature of the recepto
114                           The results reveal the necessity of having the PEGylated lipids present dur
115                   This new approach obviates the necessity of heating the plastic substrate during th
116 rforms as a tumor suppressor and underscores the necessity of identifying the conditions that enable
117  roles in decoding AS patterns, highlighting the necessity of including the epigenetic properties whe
118                     Our analysis underscores the necessity of incorporating the effects of downstream
119  minimal effects on cAMP binding, suggesting the necessity of interpreting the cAMP-dependent alloste
120 D is similar to the standard RLD but without the necessity of matching the integration interval to th
121                     These findings highlight the necessity of optimizing the Fc domain for this class
122                                 In this way, the necessity of positioning the reactive hydroxyl group
123        The presence of this pathway obviates the necessity of postulating the existence of multiple "
124 n overall yield of 53% from 4 and eliminated the necessity of preparing the diamide 13.
125 to determine helix proximity in situ without the necessity of purifying the permease.
126 previous drawbacks, which were mainly due to the necessity of relocating the plants.
127 KCC1 was present in the reaction, indicating the necessity of scaffolding the phosphatase and kinase
128 tems based on a least action method, without the necessity of simulating the steady-state distributio
129 mpact of donating solvents was attributed to the necessity of stabilizing the aluminum cation formed
130 eurobiotin-filled cone bipolar cells without the necessity of impaling them with a microelectrode.
131  effective use of eDNA methods, highlighting the necessity of understanding these parameters for use
132 ought that the slow folding may be caused by the necessity of forming this unusual beta-sheet archite
133 bodies over bNAbs to E2 CD81bs, underscoring the necessity of rigidifying this antigenic region as a
134 nt responses to this crisis, and underscores the necessity of limiting threats at their source instea
135 risk to a number of common diseases, without the necessity of going through a medical practitioner.
136                              This highlights the necessity of continuing to assess cognitive function
137                                     However, the necessity of having to conduct multiple experiments
138              Findings from this study affirm the necessity of considering women's race/ethnicity when

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