ライフサイエンスコーパス: thermal denaturation

1 o their denaturation midpoint (e.g., T m for thermal denaturation).

2 Br(2) footprinting, circular dichroism, and thermal denaturation.

3 xes in a 1 M NaCl buffer using UV absorbance thermal denaturation.

4 n unusually low change in heat capacity upon thermal denaturation.

5 cal damage that leads to an earlier onset of thermal denaturation.

6 perature bisulfite ('LowMT') and, sometimes, thermal denaturation.

7 ATP increases the activation barrier towards thermal denaturation.

8 re determined by guanidine hydrochloride and thermal denaturation.

9 uclear magnetic resonance (NMR) during their thermal denaturation.

10 nd was found to stabilize the enzyme against thermal denaturation.

11 oteins, from those folding after chemical or thermal denaturation.

12 is sufficient to produce cold, pressure, and thermal denaturation.

13 analyze the effects of salt and pH on their thermal denaturation.

14 stabilizes this heavy chain fragment against thermal denaturation.

15 6 degrees C more stable than the parallel to thermal denaturation.

16 the stability of both serum albumins against thermal denaturation.

17 higher transition temperature for rhodopsin thermal denaturation.

18 scular blood and perivascular collagen after thermal denaturation.

19 nhibitors confer enhanced protection against thermal denaturation.

20 as been known to protect other proteins from thermal denaturation.

21 lcium-binding repeats to removal of Ca2+ and thermal denaturation.

22 ecific glycosylation sites were sensitive to thermal denaturation.

23 antitumor activity and is very resistant to thermal denaturation.

24 e ones traditionally explored in chemical or thermal denaturation.

25 h point peptide binding becomes resistant to thermal denaturation.

26 in vitro and was unable to protect PC1 from thermal denaturation.

27 e low stability of the 16mer hairpin against thermal denaturation.

28 d R17H show markedly enhanced sensitivity to thermal denaturation.

29 ition of a co-solute, recorded via a delayed thermal denaturation.

30 the double-helical structure of DNA against thermal denaturation.

31 ding protects myosin against heat stress and thermal denaturation.

32 gel electrophoresis, circular dichroism, and thermal denaturation.

33 e the original proteins against chemical and thermal denaturation.

34 lding stability in bulk solution, as well as thermal denaturation, aggregation state, and biological

35 Thermal denaturation analyses by CD and differential sca

36 Circular dichroism spectra in far UV and thermal denaturation analyses demonstrate that axial lig

37 terodimer was confirmed in both chemical and thermal denaturation analyses.

38 uadruplex was studied by circular dichroism, thermal denaturation, analytical ultracentrifugation, an

39 apo-rHb, rHb-R, and rHb-A were subjected to thermal denaturation and 1H/2H exchange.

40 r characterization, using fluorescence-based thermal denaturation and a crystal structure of the isol

41 nt proteins were significantly more prone to thermal denaturation and aggregation.

42 y of the protein variants, as judged by both thermal denaturation and an unseeded in vitro oligomeriz

43 Biochemical properties such as reversible thermal denaturation and analytical gel filtration data

44 Together, the thermal denaturation and CD studies provide evidence tha

45 Thermal denaturation and chemical denaturation were not

46 Thermal denaturation and circular dichroism (CD) spectro

47 heir physical properties were studied via UV thermal denaturation and circular dichroism spectroscopy

48 Thermal denaturation and circular dichroism spectroscopy

49 Thermal denaturation and circular dichroism studies show

50 Using circular dichroism (CD), thermal denaturation and dimethyl sulfate (DMS) footprin

51 mase sequences undergo cooperative two-state thermal denaturation and display very large denaturation

52 ivity of each compound were evaluated in DNA thermal denaturation and DNase I footprinting assays, an

53 We tested six candidates by thermal denaturation and found two highly stabilizing mu

54 N370S GCase was more stable to thermal denaturation and had an increased lysosomal half

55 Thermal denaturation and imino proton solvent exchange e

56 The enzymes were characterized for their own thermal denaturation and inactivation, and they exhibite

57 and ispinesib, we report here the results of thermal denaturation and isothermal titration calorimetr

58 ty of the mutant proteins was assessed using thermal denaturation and limited digestion with protease

59 Thermal denaturation and NMR show that the RNA-1 TABS 8m

60 onA can overcome the instability issues from thermal denaturation and nonspecific electrostatic bindi

61 rand conformation using UV- and CD-monitored thermal denaturation and on nucleoside conformation usin

62 allel structure by HTS DNA in the DES, after thermal denaturation and quick cooling to room temperatu

63 Thermal denaturation and refolding monitored by CD ellip

64 Thermal denaturation and refolding on cooling appeared t

65 Thermal denaturation and refolding studies indicated tha

66 structure was formed on the DNA template by thermal denaturation and renaturation, and this hairpin

67 gel electrophoresis, circular dichroism, and thermal denaturation and show that their intra- versus i

68 Using two microbial sequence categories, thermal denaturation and target titration analyses demon

69 that are extremely resistant to chemical and thermal denaturation and that resist proteolysis by comm

70 bic cores exhibit increased stability toward thermal denaturation and unfolding by guanidinium chlori

71 rylamide gels, stabilized the domain against thermal denaturation, and induced docking to PC membrane

72 rotein, as determined by circular dichroism, thermal denaturation, and NMR studies.

73 structure as measured by circular dichroism, thermal denaturation, and size determination.

74 Chemical cross-linking, thermal denaturation, and size fractionation analyses su

75 4DNA has been obtained from CD spectroscopy, thermal denaturation, and UV-vis titration studies.

76 Residues most stable under thermal denaturation are part of a core, which is assume

77 th selenium and showed a distinct pattern of thermal denaturation as compared with GPx-1 encoded by t

78 A miniaturized thermal denaturation assay was used to screen chemical l

79 ry of cyclic polyamides were measured by DNA thermal denaturation assays and compared to the correspo

80 ate that, at pH 4.0, plasmepsin II undergoes thermal denaturation at 63.3 degrees C.

81 resistant to denaturation by low pH, but its thermal denaturation at pH 3.5 revealed a biphasic trans

82 ed to aqueous solution, pushing the solution thermal denaturation beyond the boiling point of water.

83 Results from thermal denaturation, binding energy, and recognition ex

84 Crystal structures, MANT-GTPgammaS binding, thermal denaturation, biochemical assays and sequence ho

85 nt protein is very stable under chemical and thermal denaturation but shows abnormal Ca(2+) buffering

86 cocerebrosidase that stabilize the enzyme to thermal denaturation by up to 20 K.

87 ent is consistent with stabilization against thermal denaturation caused by additional hydrogen bondi

88 We compare the thermal denaturation characteristics of double-stranded

89 UV thermal denaturation, circular dichroism, and fluorescen

90 namic analyses of gpW reveal probe dependent thermal denaturation, complex coupling between two denat

91 also significantly stabilize rhodopsin under thermal denaturation conditions, even after lipids are r

92 A thermal denaturation curve recorded at 216 nm showed a m

93 the mass spectral data is used to construct thermal denaturation curves for the detected proteins.

94 Data were compared to simulated thermal denaturation curves to determine estimates for b

95 ein-ligand binding interactions by comparing thermal denaturation data obtained in the absence and in

96 The thermal denaturation data point shows the disulfide brid

97 NMR and thermal denaturation data showed that the replacement wa

98 The thermal denaturation data were analyzed according to the

99 Based on the thermal denaturation data, GB1 stability is affected by

100 ngle-molecule force spectroscopy or existing thermal denaturation data.

101 rmediate states detectable from chemical and thermal denaturation differences in the unfolding free e

102 sized and their DNA interaction evaluated by thermal denaturation, DNA footprinting, and in vitro tra

103 CH1-Zn(2+) undergoes noncooperative thermal denaturation, does not have a near-UV elliptical

104 +)-HIF-1 alpha complex undergoes cooperative thermal denaturation, does produce a near-UV signal, and

105 hich were then characterized with respect to thermal denaturation, enzymatic stability, and fluoresce

106 Use of dynamic light scattering and thermal denaturation experiments delineates the compacti

107 Thermal denaturation experiments demonstrate that T. mar

108 Thermal denaturation experiments find that Sp significan

109 ed by means of surface plasmon resonance and thermal denaturation experiments finding that the positi

110 Thermal denaturation experiments indicate high enthalpic

111 Thermal denaturation experiments indicate surprisingly h

112 corporated in oligonucleotide sequences, and thermal denaturation experiments indicate that it is des

113 DSC and UV-monitored thermal denaturation experiments indicate that the bival

114 Moreover, thermal denaturation experiments indicated that the melt

115 Results of the thermal denaturation experiments monitored by far-ultrav

116 Thermal denaturation experiments of coiled-coil forming

117 Thermal denaturation experiments of oligonucleotide sequ

118 Analyses of solvent and thermal denaturation experiments provide the cardinal th

119 Thermal denaturation experiments provided similar result

120 Thermal denaturation experiments revealed that hm(5)rC i

121 Thermal denaturation experiments show broad transitions

122 (2) FID assay and UV thermal denaturation experiments show that 3 has a highe

123 Circular dichroism spectra and thermal denaturation experiments show that the secondary

124 Thermal denaturation experiments showed that the ternary

125 Thermal denaturation experiments showed Y131A and Y131L

126 Results from thermal denaturation experiments using circular dichrois

127 Their DNA affinity was evaluated by thermal denaturation experiments using salmon sperm DNA.

128 Thermal denaturation experiments were consistent with a

129 ONs and Invader probes are characterized by thermal denaturation experiments, analysis of thermodyna

130 f these structures was evaluated by means of thermal denaturation experiments, finding that the natur

131 folding efficiency, we performed kinetic and thermal denaturation experiments, the results of which d

132 impacting dramatically the interpretation of thermal denaturation experiments, these experimental val

133 rcalator displacement), and UV (ultraviolet) thermal denaturation experiments.

134 z8 was demonstrated by trypsin digestion and thermal denaturation experiments.

135 for DNA which was evaluated by means of DNA thermal denaturation experiments.

136 Thermal-denaturation experiments using differential scan

137 roximately linearly related to those from CD thermal denaturation for a series of four-helix bundle h

138 degrees C, spectral heterogeneity indicates thermal denaturation for the Na(+)-bound form, whereas s

139 e alpha PNAs with ssDNA has been examined by thermal denaturation, gel electrophoresis, and circular

140 turally occurring protein, with midpoints of thermal denaturation greater than 99 degrees C.

141 Thermal denaturation has irreversible destabilizing effe

142 Differential scanning calorimetry and UV thermal denaturation have been used to determine a compl

143 By thermal denaturation, HII proved to be the least stable

144 tabilized the mutants A104T and M26I against thermal denaturation, improved their ability to scavenge

145 Thermal denaturation in 150 mM KCl indicates that the CR

146 artokinase III is further stabilized against thermal denaturation in the hybrid bifunctional enzyme a

147 d, in part, by ConA's instability due to its thermal denaturation in the physiological environment (3

148 enaturant shows no heat capacity peak during thermal denaturation, indicating that the transition fro

149 uoresces upon binding to molten globules and thermal denaturation intermediates.

150 bunit analysis reveal that the first step of thermal denaturation involves dissociation of subunits I

151 Rhodopsin thermal denaturation is consistent with the two-state ir

152 displayed even by the repeat proteins whose thermal denaturation is highly cooperative, provided tha

153 itation of several individual domains due to thermal denaturation is reduced upon their fusion into m

154 Using reversible, two-state thermal denaturation, it was found that as these enzymes

155 However, close inspection of chemical and thermal denaturation kinetic experiments in fast-folding

156 Thermal denaturation kinetics demonstrated significant i

157 Thermal denaturation measurements and structural compari

158 We have carried out thermal denaturation measurements on the WT and eight mu

159 CD thermal denaturation measurements revealed that OmpC unf

160 e the experimental thermodynamics of barnase thermal denaturation: melting temperature, width of ther

161 The method is based on the commonly used thermal denaturation method in which ligand binding is r

162 The thermal denaturation method provides a means of measurin

163 hermal titration calorimetry and equilibrium thermal denaturation methods and were found to be signif

164 A wide dispersion of thermal denaturation midpoints that was observed for an

165 Helices were characterized by thermal denaturation, mixing data, and circular dichrois

166 e synthesized, purified, and investigated by thermal denaturation monitored by circular dichroism spe

167 Thermal denaturation monitored by far-UV circular dichro

168 Thermal denaturation, monitored by circular dichroism (C

169 substituted pseudoknots, using UV-monitored thermal denaturation, native gel electrophoresis, and ci

170 log interference with chemical footprinting, thermal denaturation, NMR spectroscopy, and biochemical

171 Above its melting temperature, complete thermal denaturation occurs in an activated process.

172 he hairpin starting from their ends, whereas thermal denaturation occurs stochastically.

173 udies were extended to model early stages of thermal denaturation of (Pro-Pro-Gly)10.

174 ar dichroism (CD) spectroscopy monitored the thermal denaturation of 36 heterodimers that generate si

175 The results of these experiments showed that thermal denaturation of activase in vivo occurred at tem

176 form that can be cooled efficiently to avoid thermal denaturation of antigen.

177 The reversible thermal denaturation of apo alpha-lactalbumin (alpha-LA)

178 del to describe the complex mechanism of the thermal denaturation of CcO, the obtained results were u

179 s molecular dynamics (MD) simulations of the thermal denaturation of chymotrypsin inhibitor 2 (CI2) h

180 vious molecular dynamics (MD) simulations of thermal denaturation of chymotrypsin inhibitor 2 (CI2) h

181 cluding pectin depolymerization, by reducing thermal denaturation of depolymerizing enzymes during da

182 ns of targeted DNA sequences purely based on thermal denaturation of DNA heteroduplexes without the n

183 Thermal denaturation of dodecyl maltoside solubilized Cc

184 ble unfolded (denatured) state, we study the thermal denaturation of hydrated lysozyme that occurs wh

185 ng than the pure types, the amplitude of the thermal denaturation of intracellular proteins was pract

186 In the presence of urea, the thermal denaturation of P61A FIS became concentration de

187 ere we show that, in contrast to WT FIS, the thermal denaturation of P61A FIS was incomplete and yiel

188 The thermal denaturation of proteins in skeletal muscle was

189 wnhill folding have typically focused on the thermal denaturation of proteins that fold near the spee

190 The thermal denaturation of PT S1 was inhibited by its inter

191 In vitro thermal denaturation of purified protein indicated that

192 The thermal denaturation of ribonuclease S and its fragment,

193 Thermal denaturation of the designed dimeric OmpF mutant

194 T4 bacteriophage DNA was investigated using thermal denaturation of the DNA titrated with varying co

195 ility of 1 is demonstrated by monitoring the thermal denaturation of the following double and triple

196 tions leading to stronger networks following thermal denaturation of the globular protein.

197 Thermal denaturation of the human telomerase RNA (hTR) D

198 phenotype of a specific mutation arises from thermal denaturation of the mutant enzyme, the possibili

199 The averaged free energies of urea and thermal denaturation of the paired fragments, (R16)(00)

200 van't Hoff analysis indicated that thermal denaturation of the peptide in 50% TFE containin

201 Upon thermal denaturation of the peptide, the amide-I band sh

202 Monitoring fluorescence during thermal denaturation of the protein in the presence of t

203 eaction at temperatures high enough to cause thermal denaturation of the protein substrate.

204 in homogeneous PCR screening assays in which thermal denaturation of the resulting probe-amplicon hyb

205 We found, however, that thermal denaturation of unlabelled wild-type BBL was hig

206 Q, and F92N, with increased stability toward thermal denaturation of which the F71N mutant also showe

207 ular dichroism spectroscopy, used to monitor thermal denaturations of a heterodimerizing leucine zipp

208 to individual ribbons is realized either via thermal denaturation or by addition of a DNA separator s

209 of method is then established by analysis of thermal denaturation or melting of the amplicons.

210 The thermal denaturation parameters convey the changes in st

211 vely little is known about the nature of the thermal denaturation pathway for SA.

212 Data from thermal denaturation plots and CD spectra were less conc

213 sulfoxide which, in conjunction with a short thermal denaturation, prevents renaturation of the duple

214 These results enabled us to profile the thermal denaturation process of these structures to eluc

215 lus (FdTt), we investigated its chemical and thermal denaturation processes in solution.

216 The thermal denaturation profile of MB-FB hybridized with it

217 l electrophoresis, DNA sequence analysis and thermal denaturation profile.

218 CD, NMR, and thermal denaturation profiles are consistent with the fa

219 UV thermal denaturation profiles at different pH values sup

220 Thermal denaturation profiles monitored at 450 nm (P-dC

221 Thermal denaturation profiles of several model oligonucl

222 in vitro using fluorescence polarization and thermal denaturation profiling.

223 Thermal denaturation provides additional support that Ly

224 Thermal denaturation resulted in the gradual destruction

225 n at 298 K and extrapolation to 298 K of the thermal denaturation results at high temperature.

226 o-state transition model to the chemical and thermal denaturation results, ranged from 9.4 to 148 kJ

227 Circular dichroism (CD) spectroscopy and RNA thermal denaturation revealed an increased order and sta

228 mall molecule inhibitors to protect GST from thermal denaturation revealed that compounds with differ

229 Thermal denaturation revealed that lipoprotein stability

230 Protein endothermic transitions (thermal denaturation), rheological properties (protein g

231 re as determined by near UV CD spectroscopy, thermal denaturation, sedimentation equilibrium and velo

232 On the basis of thermal denaturation, sensitivity to chemical denaturant

233 tivation by AdoMet, limited proteolysis, and thermal denaturation share a common mechanism involving

234 inding K(d) = 3.8 +/- 1.0 muM and reversible thermal denaturation showed that 5 mM calcium stabilized

235 ly by dialysis from 8 M urea and progressive thermal denaturation shows the close apposition and stru

236 tate ensemble identified in a previous 498 K thermal denaturation simulation were quenched under the

237 ding in urea was similar to that observed in thermal denaturation simulations above the protein's T(m

238 Thermal denaturation studies and footprinting experiment

239 (3) UV thermal denaturation studies and ITC experiments show th

240 Thermal denaturation studies and spectroscopic studies w

241 According to the thermal denaturation studies by UV spectroscopy, the eff

242 Thermal denaturation studies conducted optically and by

243 of these compounds by circular dichroism and thermal denaturation studies confirmed their binding mod

244 Furthermore, thermal denaturation studies detected no significant dif

245 The use of thermal denaturation studies in conjunction with UV spec

246 Circular dichroism thermal denaturation studies indicated that P6981 binds

247 Thermal denaturation studies of the SP-containing oligon

248 Thermal denaturation studies of these normal and damaged

249 Thermal denaturation studies on short DNA hairpins showe

250 Thermal denaturation studies reveal that the SR12813 lig

251 Thermal denaturation studies reveal that these hairpins

252 Circular dichroism thermal denaturation studies show that the B-SREBP-1.A-S

253 Thermal denaturation studies showed that at pH 5.0, all

254 Thermal denaturation studies showed that the collagen-li

255 Ultraviolet thermal denaturation studies showed that the duplexes de

256 However, thermal denaturation studies suggest that the originally

257 puter hardware have allowed us to extend our thermal denaturation studies to much lower temperatures.

258 ding for these two solutes are different, CD thermal denaturation studies were employed to dissect th

259 In UV thermal denaturation studies with the poly(dA) x [poly(d

260 sulting from fluorescence binding assays and thermal denaturation studies, demonstrating the ability

261 e core; this conclusion is also supported by thermal denaturation studies.

262 ivity of selected compounds was confirmed by thermal denaturation studies.

263 in different duplexes that were analyzed by thermal denaturation studies.

264 However, a thermal denaturation study using CD and differential sca

265 The midpoint of thermal denaturation (T(m)) of Fos-p1 (10 microM) is 30

266 M129C did not affect stability (midpoints of thermal denaturation, T(m) = 65-66 degrees C), whereas t

267 ociation constants determined by alternative thermal-denaturation techniques: TdF or TdCD, and also c

268 c protein directly contributes to its higher thermal denaturation temperature (Tm).

269 and DNA strand orientation can increase the thermal denaturation temperature of 17 base-pair duplexe

270 NA-binding affinity, as measured by enhanced thermal denaturation temperature of calf thymus DNA ( T

271 ine nucleosides has been synthesized and the thermal denaturation temperature of its complexes with c

272 approximately 15 degrees C difference in the thermal denaturation temperatures (T(m)) of rhodopsin an

273 ve clones were obtained that showed midpoint thermal denaturation temperatures 10-16 degrees C higher

274 o- and holo-Lf in aqueous solution displayed thermal denaturation temperatures of 71+/-0.2 and 91+/-0

275 tion characteristics (average differences in thermal denaturation temperatures of matched vs mismatch

276 The reduced thermal denaturation temperatures of the T61A, S93A, and

277 CPI was more susceptible to thermal denaturation than GCPI as determined by turbidit

278 ubstantially more stable toward chemical and thermal denaturation than Mc cytochromes b5.

279 (apo) E4 is less susceptible to chemical and thermal denaturation than the apoE3 and apoE2 domains.

280 fibres were more sensitive to oxidation and thermal denaturation than those from beta-red fibres.

281 Here we show by thermal denaturation that complementary GNA and TNA mixe

282 Further, thermal denaturation that revealed a higher stability of

283 By thermal denaturation, the MOSP(N) and MOSP(C)-like domai

284 During thermal denaturation, the mutant proteins exhibited lowe

285 large-scale simulations of protein folding, thermal denaturation, thermodynamic scan, simulated anne

286 bulk experiments (ultraviolet absorption for thermal denaturation) to analyze the differential stabil

287 d domains were higher than those selected by thermal denaturation (under both neutral and acidic cond

288 xperimental techniques (limited proteolysis, thermal denaturation, urea denaturation followed by puls

289 ween folded and unfolded conformations using thermal denaturation, urea titration, and cation-mediate

290 pecific three-stranded structure is based on thermal denaturation UV-vis and fluorescence studies.

291 '-aminouridine monomers and demonstrate, via thermal denaturation, UV-vis absorption and fluorescence

292 cutive modifications, reflected in decreased thermal denaturation values (DeltaTm, ca. 2.5-11.5 degre

293 Thermal denaturation was used to establish molecular bin

294 The various stages of collagen thermal denaturation were investigated in rat-tendon cry

295 tophan fluorescence, circular dichroism, and thermal denaturation whereas the Q67A and S247A mutants

296 so show a diverse behavior when subjected to thermal denaturation, which is exceptional as all lasso

297 Thermal denaturation, which is used to measure the globa

298 assays: specific activity and resistance to thermal denaturation, which showed that structural chang

299 In this paper, the method of thermal denaturation will be described as it has been ap

300 uations of duplex formation using ITC and UV thermal denaturation with RNA duplexes containing intern