ライフサイエンスコーパス: thermal unfolding

1 s = 11.8 kcal/mol) and undergoes cooperative thermal unfolding.

2 t cell and which can be mimicked in vitro by thermal unfolding.

3 d also from the conformations sampled during thermal unfolding.

4 strains to both guanidine hydrochloride and thermal unfolding.

5 s elegans and also subjected this protein to thermal unfolding.

6 within the MT-binding domain of SNPH against thermal unfolding.

7 the possibility of an intermediate state in thermal unfolding.

8 structures, and provided resistance against thermal unfolding.

9 a different mechanism that does not involve thermal unfolding.

10 stallography, fluorescence spectroscopy, and thermal unfolding.

11 tiary structure, as indicated by cooperative thermal unfolding and a well-dispersed NMR spectrum.

12 small heat-shock protein family, suppresses thermal unfolding and aggregation of the myosin II molec

13 NaCl concentrations, were stabilized against thermal unfolding and aggregation triggered by high HOCl

14 , we combine equilibrium NMR measurements of thermal unfolding and long molecular dynamics simulation

15 kinetic stability of discoidal lipoproteins, thermal unfolding and refolding studies were carried out

16 A scan rate dependence and hysteresis in the thermal unfolding and refolding was observed for all pro

17 With respect to thermal unfolding and the stabilization by APS, PPS-1 be

18 i has been determined using a combination of thermal unfolding and urea denaturation experiments.

19 All of the mutants display sigmoidal thermal unfolding and urea-induced unfolding curves.

20 stability of the cross-linked structures to thermal unfolding, and the sites of peptide cross-linkin

21 anning calorimetry (DSC) curves for the HisJ thermal unfolding are well described by a scheme of equi

22 The role of thermal unfolding as it pertains to thermodynamic proper

23 Apo MMP3 is remarkably stable to thermal unfolding as monitored by CD; thus the metal ion

24 s of secondary and tertiary structures after thermal unfolding at 90 degrees C, as well as preventing

25 s, undergo a relatively sharp and reversible thermal unfolding at approximately 60 degreesC.

26 pectroscopy to investigate the site-specific thermal unfolding at seven different locations in the de

27 We conclude that (1) a three-state thermal unfolding behavior appears to be conserved among

28 omplexes by sparse-matrix screening of their thermal unfolding behavior in the presence of various bu

29 t mutants are practically identical, and the thermal unfolding behavior is very similar.

30 CD studies show no difference in thermal unfolding between beta-SmTm and DeltaN-betaSmTm;

31 is well-folded and highly resistant against thermal unfolding but aggregates at elevated temperature

32 nge in negative ellipticity at 222 nm during thermal unfolding, but in the near-UV circular dichroism

33 nding stabilizes the protein as monitored by thermal unfolding by CD (50.7-54.8 degrees C) and by dif

34 , the compact folding intermediate formed by thermal unfolding can be protected against proteolysis a

35 content by 5-20% and to a large increase in thermal unfolding cooperativity.

36 ing temperature and the reversibility of the thermal unfolding curve (as measured by CD spectroscopy)

37 Further, BHBox showed a sigmoidal thermal unfolding curve with a per-residue van't Hoff en

38 ree C shift in the far UV circular dichroism thermal unfolding curve.

39 ence of helical structure and to measure the thermal unfolding curve.

40 he method includes the automated analysis of thermal unfolding curves based on a biophysical unfoldin

41 In contrast, thermal unfolding curves determined via CD indicate that

42 2,2, 2-trifluoroethanol) back to water, the thermal unfolding curves have been measured by circular

43 The change in the apparent cooperativity of thermal unfolding curves in concentrated TFE solutions r

44 folding predicts some dispersion of measured thermal unfolding curves of individual residues at pH5.3

45 Thermal unfolding curves of the five 13-residue peptides

46 halpy of helix formation calculated from the thermal unfolding curves varies widely among the five pe

47 um chloride induces cold denaturation in the thermal unfolding curves, providing a reasonably well-de

48 ure is formed cooperatively, as indicated by thermal unfolding curves.

49 d not only to be comparable to previous FTIR thermal unfolding data but also to have a denatured-stat

50 delta Cp, was measured by global analysis of thermal unfolding data collected at a number of guanidin

51 s fit should be applicable to isothermal and thermal unfolding data for all proteins with similar com

52 Thermal unfolding data on 158 backbone and side-chain pr

53 o-state model to far- and near-UV CD and DSC thermal unfolding data.

54 se determined from the two-state analysis of thermal unfolding data.

55 are assigned by inspection and used to study thermal unfolding equilibria over the entire transition

56 Thermal unfolding experiments indicate that a hydrophobi

57 Thermal unfolding experiments monitored by steady state

58 Thermal unfolding experiments monitored by ultraviolet s

59 e effects (KIEint) on the chemical step, and thermal unfolding experiments of both l- and h-DHFR show

60 nge the overall secondary structure, whereas thermal unfolding experiments revealed that fluorescent

61 Thermal unfolding experiments show that the complex has

62 Thermal unfolding experiments suggest that this process

63 Thermal unfolding experiments suggested that removal of

64 Urea denaturations and thermal unfolding experiments were used to measure the f

65 In vitro thermal unfolding experiments, as well as assessment of

66 elix bundle by -0.8 kcal/mol, evaluated from thermal unfolding experiments.

67 g structure is -1.2 kcal/mol, evaluated from thermal unfolding experiments.

68 tants were as stable as wild-type decorin in thermal unfolding experiments.

69 peptide coiled-coil trimer, as evaluated by thermal unfolding experiments.

70 tence of a thermodynamic intermediate of the thermal unfolding/folding process, termed III h, which i

71 Thermal unfolding followed by differential scanning calo

72 Aggregation accompanies thermal unfolding for both proteins under most condition

73 troscopy over a range of temperature through thermal unfolding has been applied to the low-spin, ferr

74 Thermal unfolding has been studied as a function of W0,

75 Some thorough studies of thermal unfolding have been carried out; however, protei

76 dynamical regime is attained in proximity of thermal unfolding in all solvents that we tested.

77 ly identical, indicating that stabilized MDH thermal unfolding intermediates are not determined by th

78 havior of human versus murine TNF-alpha upon thermal unfolding is due to differences in the solubilit

79 for both proteins under most conditions, but thermal unfolding is reversible and two-state for EP3 at

80 Thermal unfolding is stochastic, whereas mechanical unfo

81 luctuations of proteins exists, beyond which thermal unfolding is triggered.

82 Thermal unfolding measurements by circular dichroism ind

83 Equilibrium thermal unfolding measurements of the D-Arg peptide moni

84 Thermal unfolding of 9+ ions of cytochrome c proceeds th

85 The midpoint of thermal unfolding of a 1 M protein solution (T degree va

86 The thermal unfolding of a 40-residue helix-turn-helix subdo

87 -cell NMR to follow at atomic resolution the thermal unfolding of a beta-barrel protein inside mammal

88 ifferent functional domains, we analyzed the thermal unfolding of a homopentameric LGIC, the 5-hydrox

89 We study the thermal unfolding of a protein, probed by two protein na

90 The thermal unfolding of a series of 6-, 10-, and 14-mer cyc

91 The enthalpy for thermal unfolding of AcWL(5) beta-sheets in the membrane

92 In addition, we analyze the thermal unfolding of alpha-t, IgE, and their complex.

93 ll as real experimental amide I' spectra for thermal unfolding of an alpha-helical peptide.

94 or coordination in MCO proteins, we assessed thermal unfolding of apo and metallated forms of Fet3p b

95 At protein concentrations <5 mg/ml, thermal unfolding of apoA-1 is resolved as an extended p

96 Here, we investigate the thermal unfolding of beta2S-PDZ at different pH and urea

97 The kinetics of thermal unfolding of both of these enzymes were consiste

98 Analysis of the chemical and thermal unfolding of both proteins indicates a value of

99 We show here that thermal unfolding of BSF occurs in two distinct steps co

100 Thermal unfolding of discoidal complexes of apolipoprote

101 The thermodynamic parameters of the thermal unfolding of each of the labeled segments were o

102 s including the volume change resulting from thermal unfolding of each protein.

103 structure was also suggested by studying the thermal unfolding of g3p: the TolA binding site on D1, w

104 f N-linked carbohydrate increases the Tm for thermal unfolding of gOMCHI1 over rOMCHI1 by 2-4 degrees

105 In the virion the thermal unfolding of gp4 is prevented by the interaction

106 We reexamined the thermal unfolding of lipid-free apoA-1 in low-salt solut

107 The thermal unfolding of MW is reversible with a melting tem

108 The thermal unfolding of Nkx2.5(C56S) at pH 6.0 or 7.4 is a

109 CD spectroscopy measuring the thermal unfolding of NKX3.1 constructs showed a 2 degree

110 Thermal unfolding of PE, as evidenced by changes in alph

111 ay represent the first example of reversible thermal unfolding of peptides in membranes, help to defi

112 We studied the thermal unfolding of recombinant ERalpha by circular dic

113 We simulate the thermal unfolding of rhodopsin by breaking the native-st

114 Two mechanisms have been proposed for the thermal unfolding of ribonuclease S (RNase S).

115 ins within rNK1 was tested by monitoring the thermal unfolding of rNK1/K1 when in the presence of the

116 uctural features, at an atomic level, of the thermal unfolding of Ros87 and compared them to the beha

117 Thermal unfolding of the 5-hydroxytryptamine receptor oc

118 We here compare thermal unfolding of the apo and holo forms of Desulfovi

119 The best-fitted values for the thermal unfolding of the apo-protein were 60.9 +/- 0.2 d

120 Thermal unfolding of the C-terminal domain occurred over

121 The thermal unfolding of the cleaved ribozyme was also exami

122 tinct and independent transitions during the thermal unfolding of the enzyme.

123 protein-ligand complex (NL<==>N + L) and the thermal unfolding of the free protein (N<==>U).

124 these sites contribute to stability against thermal unfolding of the isolated VWF A2 domain.

125 Chemical and thermal unfolding of the L93A monomer followed by circul

126 Thermal unfolding of the monomeric subunits occurred wit

127 l scanning calorimetry (DSC) showed that the thermal unfolding of the myosin II rod is reversible and

128 Reversible thermal unfolding of the nonphosphorylated N-terminal do

129 We investigated the thermal unfolding of the peripheral subunit binding doma

130 In contrast, the thermal unfolding of the proposed major and highly stabl

131 Thermal unfolding of the T domain, measured by circular

132 Concurrently, thermal unfolding of these forms of EI has been monitore

133 Moreover, the thermal unfolding of these proteins does not result in t

134 The thermal unfolding of these proteins is > 90% reversible,

135 The guanidine-HCl induced unfolding and thermal unfolding of these proteins were studied to char

136 h alpha-helical proteins and the cooperative thermal unfolding of these proteins.

137 ism and infrared data also indicate that the thermal unfolding of these two monomeric coiled-coils ca

138 quilibrium IR measurements indicate that the thermal unfolding of this beta-hairpin is fairly broad.

139 We studied the thermal unfolding of ubiquitin in the presence of differ

140 nd transient conformational changes upon the thermal unfolding of ubiquitin were investigated with no

141 Thermal unfolding of variant apoA-I/DMPC complexes monit

142 cence-based dye binding assay to measure the thermal unfolding of wild-type (wt), DeltaLid, and S17D

143 The thermal unfolding of zwittergen-solubilized class 2 trim

144 ough the protein does not exhibit reversible thermal unfolding, one can determine its stability param

145 l features at 37 degrees C, with significant thermal unfolding only occurring at temperatures >or=50

146 e thermal stability and the cooperativity of thermal unfolding over a wide pH range (0.9-7.5).

147 The site-specific thermal unfolding probed by (13)C isotopically edited IR

148 69 of the 75 interresidue sites, shows that thermal unfolding proceeds via a diversity of intermedia

149 The global thermal unfolding process and the partial or local struc

150 in CP thermal stability, we have probed the thermal unfolding process as a function of scan rate of

151 The thermal unfolding reactions are irreversible and, for th

152 Thermal unfolding reactions at pH 7.4 are reversible and

153 (MBP-(85-99)) showed increased stability to thermal unfolding relative to the empty DR2-derived RTLs

154 explain these observations we used atomistic thermal unfolding simulations to identify ensembles whos

155 roscopy, analytical ultracentrifugation, and thermal unfolding studies indicate that the A and B pept

156 Thermal unfolding studies show that the CPmac mutations

157 Urea denaturation and thermal unfolding studies show that the N136A mutant enz

158 A thermal unfolding study of the 45-residue alpha-helical

159 at pH 3.5, with a > or = 20 degrees C higher thermal unfolding temperature at all pHs.

160 The midpoint thermal unfolding temperature of the fluorinated peptide

161 gimes whose breakpoint occurs near the first thermal unfolding temperature of the mAb domain structur

162 CR decreased the apparent thermal unfolding temperature of the protein.

163 observed little ionic strength effect on the thermal unfolding temperature, Tm, values in these syste

164 revealed that A2-VicCC was more resistant to thermal unfolding than A2-DeltaCC.

165 e sensitive to acid-induced denaturation and thermal unfolding than sbR(WT).

166 sensitive fluorescent dye to monitor protein thermal unfolding, the ligand-binding affinity can be as

167 SD) matrix of the conformations sampled in a thermal unfolding trajectory, have also been used to ide

168 in embedded in different environments as the thermal unfolding transition is approached.

169 However, the cooperativity of the thermal unfolding transition is reduced by the presence

170 dominated by alpha-helices and a cooperative thermal unfolding transition of 49 degrees C, features c

171 es from that of the holoenzyme: (1) the main thermal unfolding transition of the apo-HCA II is lowere

172 The thermal unfolding transition temperature (T(m)) of rhodo

173 The thermal unfolding transition temperature of this protein

174 label was exploited to probe the equilibrium thermal unfolding transition using temperature-dependent

175 beta-sheet undergoes a broad but cooperative thermal unfolding transition with a midpoint at approxim

176 this domain, psbd41, undergoes a cooperative thermal unfolding transition with a tm of 54 degrees C.

177 ding a tendency to aggregate, noncooperative thermal unfolding transition, and a loosely packed core.

178 e proteins exhibited a cooperative two-state thermal unfolding transition, and DR2-derived RTLs with

179 000 s-1 near the midpoint of the equilibrium thermal unfolding transition.

180 ed it to be folded with a weakly cooperative thermal unfolding transition.

181 ure with a reversible and highly cooperative thermal unfolding transition.

182 ry (DSC), several laboratories find that the thermal unfolding transitions of alpha alpha and beta be

183 Consistent with this data, the shape of thermal unfolding transitions varies from asymmetric for

184 ees C) at the approximate midpoints of their thermal unfolding transitions were found to be similar.

185 resonance spectroscopy, we demonstrate that thermal unfolding under amyloidogenic solution condition

186 The T(m) for thermal unfolding was inversely related to acyl chain pa

187 To probe the transition state of ubiquitin thermal unfolding, we examine unfolding dynamics and kin

188 The energetics of thermal unfolding were also evaluated by differential sc

189 Conformational dynamics during thermal unfolding were probed by hydrogen/deuterium (H/D

190 ncomplete, cooperative, partially reversible thermal unfolding with increased unfolding temperature a

191 the protein undergoes reversible, two-state thermal unfolding with Tm = 70.0 +/- 0.3 degrees C and a