ライフサイエンスコーパス: thermolysin

1 entration of proteolytic enzymes (trypsin or thermolysin).

2 r structures from different X-ray studies of thermolysin.

3 is of fragments produced on proteolysis with thermolysin.

4 at of an insect metalloprotease inhibitor in thermolysin.

5 C1 and C2 collagenases and ChNP in place of thermolysin.

6 th the active-site region related to that of thermolysin.

7 rial zinc metalloendopeptidases, typified by thermolysin.

8 e, human serum albumin, gamma-globulins, and thermolysin.

9 similar to those of metalloproteases such as thermolysin.

10 f the general zinc-dependent metalloprotease thermolysin.

11 ), an inhibitor bound to human rhinovirus 14 thermolysin.

12 eptidase pepsin, and with the zinc peptidase thermolysin.

13 metalloamidases, like carboxypeptidase A and thermolysin.

14 on binding Tva, as assayed by sensitivity to thermolysin.

15 tion identical to that of the zinc peptidase thermolysin.

16 rocrystals of Geobacillus stearothermophilus thermolysin (2.2-A structure), Thermosynechococcus elong

17 e enzyme, with a Ki of 3 x 10(-9) M, whereas thermolysin and a metalloproteinase from A. flavus were

18 ates of archetypical zinc hydrolases such as thermolysin and carboxypeptidase A.

19 nd chemoselective: various proteases such as thermolysin and chymotrypsin catalyze amine acyl exchang

20 d statistically better results (P < 0.01 for thermolysin and P < 0.05 for GP datasets) than the CoMFA

21 e hydrodabcyl-Ser-Phe-EDANS by the proteases thermolysin and papain.

22 Its structural comparison with thermolysin and the available pool of LC structures reve

23 l results, within this series, of binding to thermolysin and to a lesser extent to NEP was observed.

24 step enzymatic modification method involving thermolysin and transglutaminase.

25 approaches use the X-ray structures of ZFpLA-thermolysin and ZGpLL-thermolysin structures.

26 equally susceptible to digestion by trypsin, thermolysin, and proteinase K.

27 man leukocyte elastase, pancreatic elastase, thermolysin, and Pseudomonas elastase.

28 For example, carboxypeptidase A, thermolysin, and sonic hedgehog possess the same ligand

29 143 catalytic base in B. thermoproteolyticus thermolysin, and the E181A VanX mutant has no detectable

30 rotein are readily hydrolyzed by trypsin and thermolysin, around residues 127-128, indicating that th

31 Inhibition of thermolysin by the silanediol (K(i) = 41 nM) was compara

32 s released during digestion of repolymerized thermolysin-casein hydrolysates had no immuno-reactivity

33 In kinesin, a thermolysin cleavage at L154 in L8 is protected in AMPPN

34 conformational changes, it can increase the thermolysin cleavage resistance up to 50-fold depending

35 We show that thermolysin-cleaved MccJ25 and MccJ25 lacking amino acid

36 ometry and N-terminal sequencing showed that thermolysin cleaves the peptide bond between Thr92 and A

37 To address this question, we utilized a thermolysin-derived product of the alpha1alpha2alpha1 ne

38 r data and structural analysis of intact and thermolysin-digested MccJ25 suggest that distinct region

39 mutations in the first epitope identified by thermolysin digestion (Ssa2128-132A3) significantly redu

40 Thermolysin digestion and immunoprecipitation experiment

41 astellin binds to (III)11, where it enhances thermolysin digestion of (III)11.

42 ovel molecular strain typing assay that used thermolysin digestion of caudal medulla samples to produ

43 ronger than actin-binding domain produced by thermolysin digestion of native alpha-actinin (residues

44 Thermolysin digestion of the isolated omeprazole-labeled

45 Proteomic analysis using thermolysin digestion of three predominant non-native mo

46 f the Rieske iron-sulfur protein, removed by thermolysin digestion, into PL vesicles together with mi

47 Maltose binding protein, a survivor from thermolysin digestion, was characterized by in vitro bio

48 reased the sensitivity of the polypeptide to thermolysin digestion.

49 of Delta G(proteolysis), we determined that thermolysin digests this protein through a local fluctua

50 mbinant leptospiral metalloprotease from the thermolysin family cleaved C3 in serum and could be one

51 metric similarity to the active sites of the thermolysin family.

52 family and a 42-kDa metalloproteinase of the thermolysin family.

53 some non-specific enzymes such as pepsin or thermolysin for proteolysis in proteomic study, in that

54 We have mapped thermolysin, for which experimental mapping results are

55 ive binding mode for ordinary substrates and thermolysin, forces reinterpretation of previous mechani

56 l coordination identical to that observed in thermolysin from Bacillus thermoproteolyticus.

57 ease (pepsin at pH>2) and metalloproteinase (thermolysin) have strong prospects for use in simultaneo

58 Ile39Glu and Ile39Lys were cleaved by thermolysin in both the absence and presence of Tva.

59 A tight-binding thermolysin inhibitor, Cbz-Phe-psi[PO2NH]-Leu-Ala (ZFpLA

60 dentified by comparing this structure with a thermolysin-inhibitor complex structure.

61 A previously studied congeneric series of thermolysin inhibitors addressing the solvent-accessible

62 investigate the thermodynamic fingerprint of thermolysin inhibitors featuring terminal charged ammoni

63 ifference between conformationally different thermolysin inhibitors ZFpLA and ZGpLL is estimated usin

64 /or inhibitors of hen egg-white lysozyme and thermolysin interact with the same side chains identifie

65 een applied to hen egg-white lysozyme and to thermolysin, interacting with eight and four different l

66 rates, which indicates that their binding to thermolysin is largely productive, unlike normal peptide

67 temperatures using the thermophilic protease thermolysin led to selective removal of the zeins, where

68 rity with MPs of the gluzincin tribe such as thermolysin, leukotriene A4 hydrolase relatives, and cow

69 Bacillus anthracis lethal toxin contains the thermolysin-like active-site and zinc-binding consensus

70 ight chain structures, including a conserved thermolysin-like core inserted between structurally dist

71 The first (designated Npr599) is a thermolysin-like enzyme highly homologous to bacillolysi

72 di-chain protein composed of an N-terminal, thermolysin-like metalloprotease light chain domain (LC/

73 rotease (Mpl) of Listeria monocytogenes is a thermolysin-like protease that mediates the maturation o

74 predicted preproenzyme structure typical of thermolysin-like proteases and is distantly related to B

75 boxypeptidase A makes only Ndelta1 contacts, thermolysin makes only Nepsilon2 contacts, and sonic hed

76 Collagenase I, II, Liberase MTF C/T, thermolysin/neutral protease share similar cleavage site

77 the novel peptide substrate is collagenase, thermolysin/neutral protease specific and can be applied

78 , II, Liberase MTF C/T, collagenase NB1, and thermolysin/neutral protease, which was significantly en

79 ent with five different proteases: Alcalase, Thermolysin, Neutrase, Flavourzyme and PTN.

80 Cleavage by thermolysin of N-(4-methoxyphenylazoformyl)-L-leucyl-L-l

81 NP) from Bacillus thermoproteolyticus rokko (thermolysin) or Ch (ChNP).

82 In this work, we show that thermolysin, or an endogenous activity present in R. cap

83 t by clostripain, trypsin, neutral protease, thermolysin, or elastase.

84 es, including trypsin, chymotrypsin, pepsin, thermolysin, papain, and calpain.

85 te calcium, or magnesium or zinc ions [e.g., thermolysin (PDB code: 3tln), mannose-binding protein (2

86 Data presented for four thermolysin phosphonamidate inhibitors demonstrate that

87 teolysis of purified S. aureus DNA ligase by thermolysin produced products with apparent molecular ma

88 alysis of isolated chloroplasts treated with thermolysin protease.

89 aspartic (HIV protease) and metallo (ACE and thermolysin) proteases.

90 ormation of the correct membrane-associated, thermolysin-protected assembly is strictly dependent of

91 5 between residues Gln197-Arg198 and, unlike thermolysin, recognizes an extended region of SNAP25 for

92 within a standard triple helix, trypsin and thermolysin resistance indicated a tightly packed struct

93 The combined analyses of C2 fragments and thermolysin-resistant PrP species within caudal medulla,

94 y, pea and wheat, with both chymotrypsin and thermolysin, resulted in hydrolysates, which are efficie

95 ligase from Bacillus stearothermophilus with thermolysin results in two fragments which were resistan

96 ly hydrolyzed with chymotrypsin, trypsin, or thermolysin retained about 80%, 30%, and 20% of the orig

97 Temperature-dependent proteolysis with thermolysin revealed a stable core region within the N-

98 ntial role of apo(a) O-glycans in protecting thermolysin-sensitive regions of the polypeptide.

99 change in that domain that exposed a cryptic thermolysin-sensitive site.

100 Soybean protein hydrolysate digested by thermolysin showed an IC50 value of 53.6 mug/mL, decreas

101 ay structures of ZFpLA-thermolysin and ZGpLL-thermolysin structures.

102 placing the presumed tetrahedral carbon of a thermolysin substrate with a silanediol group, resulting

103 oth of these strategies to produce imprinted thermolysin, subtilisin, and lipase TL possessing up to

104 cking studies were carried out in a model of thermolysin (TLN) using the MACROMODEL and QXP modeling

105 Thermolysin treatment also dissociates F185 from the det

106 HA2 (residues 24-185), which is analogous to thermolysin treatment of HA in the low-pH-induced confor

107 Thermolysin treatment of the aggregated protein removed

108 titions, sensitivity to pre- and post-import thermolysin treatment, photochemical cross-linking and o

109 es: (k(cat)/K(m))(SSP) > (k(cat)/K(m))(THP) (thermolysin, trypsin, and MMP-13) or (k(cat)/K(m))(THP)

110 However, a 140-kD thermolysin TSP fragment, containing the carboxy-termina

111 o acid sequences revealed the HEXXH motif of thermolysin-type metalloproteases and an HHH polyhistidi

112 ase 1), MMP-13 (collagenase 3), trypsin, and thermolysin using triple-helical peptide (THP) and singl

113 f the silanediol bound to the active site of thermolysin was found to have a conformation very simila

114 Proteolytic digestion of MutY using thermolysin was found to produce two relatively stable f

115 Also, one type of HEXXH-containing thermolysin was inactive in the C. albicans assay, where

116 Potent inhibition of both NEP and thermolysin was obtained.

117 silanediol inhibitor of the metalloprotease thermolysin was prepared for comparison to a known phosp

118 idate inhibitors (1-3) to the zinc peptidase thermolysin was probed by varying the solvent compositio

119 Thermolysin was the enzyme which yielded the hydrolysate

120 s released during digestion of repolymerized thermolysin-whey protein hydrolysate had less than 5% im

121 proteases (e.g., trypsin, proteinase K, and thermolysin) while obeying Michaelis-Menten kinetics.

122 1' specificity pocket of the metalloprotease thermolysin with purposefully designed ligands using hig

123 oMFA with the use of ILP on the well-studied thermolysin zinc protease inhibitor dataset and a glycog