ライフサイエンスコーパス: thermostability

1 d catalytic activity, substrate binding, and thermostability.

2 ated dimer interface possibly reflecting its thermostability.

3 glycosylation events determined increases in thermostability.

4 le others such as the K34Q mutant reduce its thermostability.

5 bB possesses a unique fold that imparts high thermostability.

6 were shown to possess significantly enhanced thermostability.

7 site lid structure, while fully maintaining thermostability.

8 ated dimer interface possibly reflecting its thermostability.

9 of 3-7 to assess changes in C(H)2 and C(H)3 thermostability.

10 vitro engineering of proteins with improved thermostability.

11 s in using D-amino acids to increase protein thermostability.

12 roperties and dramatically increased kinetic thermostability.

13 t changes in secondary structure content and thermostability.

14 cium for binding to calreticulin and reduces thermostability.

15 , which is in contrast with the consensus on thermostability.

16 protein's energy landscape that lead to such thermostability.

17 tiary structure leading to a decrease of its thermostability.

18 ting in approximately 14 kJ/mol of increased thermostability.

19 eds more CBH II chimeras share this superior thermostability.

20 sequence that adds 8.5 degrees C to chimera thermostability.

21 eases IDE oligomerization, and decreases IDE thermostability.

22 taining satisfactory specific activities and thermostability.

23 y inefficient turnover of 5FC and/or limited thermostability.

24 wed reduced recognition of CD4 and increased thermostability.

25 hown to decrease K(d) surface expression and thermostability.

26 salt and temperature optima for activity and thermostability.

27 that contribute to TFPA's increased kinetic thermostability.

28 (short isoform) variants exhibiting improved thermostability.

29 ries where 50% of the variants have improved thermostability.

30 e LacY with respect to substrate binding and thermostability.

31 adaptation on "sequence-based" mechanism of thermostability.

32 gree, two fundamental physical mechanisms of thermostability.

33 ructural features as responsible for protein thermostability.

34 er number of interactions is responsible for thermostability.

35 for ligand binding, enzyme processivity, and thermostability.

36 fold decrease in k(cat) and a severe loss in thermostability.

37 shion consistent with the slight decrease in thermostability.

38 polyclonal immune sera without altering its thermostability.

39 as calcium often play a key role in protein thermostability.

40 loop that confers proteinase resistance and thermostability.

41 ts have interesting implications for protein thermostability.

42 butions of electrostatic interactions to the thermostability.

43 peptide repertoire demonstrated by increased thermostability.

44 he importance of hydrophobic interactions in thermostability.

45 on the surface have little effect on protein thermostability.

46 ved vaccine immunogenicity, dose sparing and thermostability.

47 hout affecting VcDsbA secondary structure or thermostability.

48 especially if the desired scaffold is of low thermostability.

49 e discovery of mutations that enhance enzyme thermostability.

50 mino acids or positions that likely underlie thermostability.

51 rrelated with their ability to increase NBD1 thermostability.

52 of K(m), k(cat), buffer/serum half-life, and thermostability.

53 binding affinity, proteolytic stability, and thermostability.

54 a molecular basis of NTR contribution to its thermostability.

55 es C to 70 degrees C, indicating fairly high thermostability.

56 ing, and within the affibody to increase its thermostability.

57 can form a B-form structure and exhibit high thermostability.

58 er-onset mutations which exhibited increased thermostability.

59 hroughout SERT, 10 were found to improve its thermostability.

60 of the presence of CG, EPI and/or AA on PPO thermostability.

61 n a mixture of subpopulations with different thermostabilities.

62 ed receptor (StaR), minimally engineered for thermostability, additional single mutations are then ad

63 riods revealed the presence of a gradient of thermostability along the collagen molecule with thermol

64 We also present thermostability analysis of GCase at pH 7.4 and 5.2 usin

65 five homologous RNase H proteins of varying thermostabilities and enzymatic activities from organism

66 on pairing and, in return, leads to enhanced thermostability and a dramatically reduced (up to 10-30

67 We also observed an improvement in thermostability and a reduction in CD4 affinity.

68 The high thermostability and activity of agave Rca mapped to the

69 This increase in thermostability and alkali tolerance translates to a 4,0

70 h) and spiroiminodihydantoin (Sp) reduce the thermostability and alter the folding of telomeric quadr

71 1984Val variant showing a 2-fold increase in thermostability and an approximately 4- to 8-fold increa

72 des the structural basis for the increase in thermostability and an understanding how N268D and N239Y

73 Our thermostability and crystal structure studies indicate t

74 quantitative prediction of cellulase chimera thermostability and efficient identification of stabiliz

75 loid strains that are characterized by lower thermostability and higher frangibility in vitro and str

76 analysis of F45betaY showed that it enhanced thermostability and increased affinity by 60-fold.

77 Correlation of thermostability and inhibition potency suggests that bet

78 CysK significantly increases CdiA-CT(EC536) thermostability and is required for toxin interaction wi

79 ins retained tetravalent streptavidin's high thermostability and low off-rate.

80 rmed small plaques, had dramatically reduced thermostability and lower infectivity.

81 n vitro, the mutant actins exhibited altered thermostability and nucleotide exchange rates, indicatin

82 ally modified starch showed a lower range of thermostability and recrystallization temperatures.

83 N-linked glycans of the VWF A2 domain affect thermostability and regulate both the exposure of the AD

84 ific molecules in Escherichia coli, improved thermostability and resistance to proteases.

85 Correspondingly, K(d) thermostability and surface expression were increased by

86 peptides, whereas the EGK peptide has lower thermostability and the DGK peptide is the least thermos

87 the sequence and structural basis of protein thermostability and the mechanistic principles of protei

88 The thermostability and the overall secondary structure cont

89 nteraction between the intrinsic property of thermostability and the reactivity of buried free-cystei

90 ift unfolding revealed a correlation between thermostability and the severity of hyperglycemia in the

91 is proposed to be a basis for the disparate thermostabilities, and the corresponding free energy dif

92 Reptin mutants with altered oligomerization, thermostability, and AGR2 binding properties.

93 SdAb possess good solubility, thermostability, and can refold after heat and chemical

94 In this study, the hygroscopicity, thermostability, and density of alkylaminium sulfates (A

95 es that contribute to extreme halophilicity, thermostability, and high detoxification capacity, sugge

96 The improved antigenicity, thermostability, and immunogenicity of DS-SOSIP.4mut sug

97 n dependence for cell surface expression and thermostability, and its enhanced susceptibility to path

98 cluding well-established chemical synthesis, thermostability, and low production cost.

99 positional bias has resulted in reduced tRNA thermostability, and may have altered aminoacyl-tRNA syn

100 body formats maintain high expression level, thermostability, and protease resistance.

101 ered substrate specificity profile, enhanced thermostability, and remarkable reactivity toward the de

102 ectious virions that were similar in number, thermostability, and sedimentation characteristics to th

103 ts suggest that NTR plays a role in XynCDBFV thermostability, and the Cys-4/Cys-172 disulfide bond is

104 N protein of NDV is a crucial determinant of thermostability, and the HN gene from a thermostable NDV

105 ownstream of PET, increases protein folding, thermostability, and tolerance to limited proteolysis.

106 there was a correlation between fitness and thermostability, and we observed that the correlation wa

107 The variations in the protein thermostability are correlated with those in the near-UV

108 dings for engineering proteins with enhanced thermostability are discussed.

109 While the overall structure and thermostability are not altered, a subtle increase in th

110 ealed that its overall protein structure and thermostability are not compromised.

111 mber of structural features that can enhance thermostability are revealed.

112 mesophile Escherichia coli revealed that the thermostability arises in part from an unusually low cha

113 plexes with RNA showed slightly lower duplex thermostability as compared to that of the more rigid 3'

114 These dimers have the same thermostability as MLV genomic RNA dimers; like them, th

115 McjD resulted in reduced ATPase activity and thermostability as shown by circular dichroism, both of

116 on of metal ions to reach the same levels of thermostability as the wild-type protein.

117 A thermostability assay indicated that purified tAlv-a1-pH

118 A thermostability assay suggested the modified viruses wer

119 mutagenesis coupled to a radioligand-binding thermostability assay that can be applied to receptors,

120 entification of thermostable mutants using a thermostability assay that is based on binding of an (12

121 ed their stability with a fluorescence-based thermostability assay that monitors protein unfolding wi

122 ocols and advice are given on how to develop thermostability assays for MPs and how to combine mutati

123 Here, we use thermostability assays to demonstrate that the carrier i

124 monstrated by reconstitution experiments and thermostability assays, indicating that the lipid has an

125 favorable kinetic properties, and excellent thermostability at 37 degrees C.

126 yruvate dehydrogenase complex assay; (ii) in thermostability at 37 degrees C; (iii) in the mechanism

127 bilisation process resulted in higher enzyme thermostability at 45 and 50 degrees C.

128 y structure, which is required for increased thermostability but which also prevents active modulatio

129 of T(m) = 52 degrees C, enhanced the protein thermostability by 36 degrees C (T(m) = 88 degrees C), w

130 his site optimizes both antigen affinity and thermostability by modulating the interdomain conformati

131 st provide the general background of protein thermostability by specifically focusing on the structur

132 ucture also indicates a structural basis for thermostability by Taq FBP aldolase.

133 Here we show that a chimera's thermostability can be predicted from the additive contr

134 However, thermostability can vary between isolates regardless of

135 or specific properties of an enzyme, such as thermostability, catalytic activity and substrate specif

136 e enzyme performance based on differences in thermostability, cellulose-binding domain targeting, and

137 These thermostability changes are sufficiently divergent to al

138 t GCK proteins were analyzed for kinetic and thermostability characteristics and assigned a relative

139 53C-T142C/T46P mutant xylanase had excellent thermostability characteristics and could be a prospecti

140 ly higher pH of HA activation and reduced HA thermostability compared to the corresponding wild-type

141 HA3-SS has increased thermostability compared to wild-type HA, and binding of

142 ermine whether the underlying mechanisms for thermostability correlate with the changes in T(m), we m

143 We found a large loss in thermostability correlating with the loss of the lipid b

144 For the chloride salts, protein thermostability decreases as the size of the peptide inc

145 biocatalysts featuring dramatically enhanced thermostability (DeltaTm = +18.0 degrees C and DeltaT50

146 However, AAO presented different thermostability depending on the electric field strength

147 n with the starch fraction of maize kernels, thermostability desirable in maize processing, and resis

148 ealed impaired DNA binding affinity, reduced thermostability, diminished exonuclease activity, defect

149 pical pharmacokinetics in rodents and retain thermostability, enabling efficient knobs-into-holes bis

150 nce properties, including functional status, thermostability, enzyme activity, and ligand binding aff

151 and the application of selection methods to thermostability evolution have enabled the screening of

152 A thermostability experiment revealed the two enzymes to b

153 Therefore, improving chABC thermostability facilitates minimally invasive, sustaine

154 signed based on mathematical modeling of the thermostabilities for the first set of chimeras.

155 We also observed an increase in thermostability for a looped promoter structure.

156 al hybridization without compromising duplex thermostability has proven challenging.

157 Studies of the structural basis of protein thermostability have produced a confusing picture.

158 ve state among which two exhibit an apparent thermostability higher than WT and M23 (a receptor varia

159 ion concerning structure, stoichiometry, and thermostability; how pRNA studies have led to the genera

160 sylation at the catalytic domain affects its thermostability; however, glycosylation of M2 had no eff

161 , D371A, was found to confer (i) substantial thermostability, (ii) an infectivity defect that followe

162 to be in its antagonist conformation and its thermostability improved by earlier limited mutagenesis.

163 n structures enabled the analysis of protein thermostability in a network context.

164 MfdN has large effects on MfdC activity and thermostability in cis, these effects are not observed i

165 expressed in P. pastoris presented increased thermostability in comparison with their unglycosylated

166 actions provides a simple means for evolving thermostability in functional RNAs.

167 r results provide a clear picture of protein thermostability in one species, and a framework for futu

168 or Lys mutations in PsbO-Asp157 modify PsbO thermostability in solution, which is consistent with th

169 gp13 exhibits enhanced thermostability in the DNA-filled capsid.

170 cular disulfide bond contributes significant thermostability in vitro.

171 e been applied to the evolution of increased thermostability, including the use of combinatorial desi

172 Although duplex thermostability increases with CG content, the enthalpic

173 The extent to which thermostability influences the location of protein fragm

174 idues that influence antibody expression and thermostability is often needed to move promising therap

175 owever, the genetic basis underlying the NDV thermostability is poorly understood.

176 ss, providing evidence that metabolic enzyme thermostability is rate-limiting at superoptimal tempera

177 yB-Cph1, which based on their small size and thermostability may be useful as cell biological reporte

178 properties such as enzyme activity, protein thermostability, mutation position or degree of change i

179 model accurately (R(2) = 0.88) describes the thermostabilities of 54 characterized members of a famil

180 In this study, we analyzed the thermostabilities of 7 H2N3 viruses isolated from mallar

181 ere we attempt to understand how the diverse thermostabilities of bacterial ribonuclease H1 (RNH) pro

182 Whereas the thermostabilities of HjCel3A and Pp-HjCel3A are the same

183 ntributions of various loop sequences to the thermostabilities of parallel-stranded G-quadruplexes.

184 Assays of thermostabilities of the Arg75Cys and Arg789Cys collagen

185 NMR structures indicated that the different thermostabilities of the two 1:2:1 parallel c-MYC G-quad

186 ate the ability of our method to improve the thermostability of a well-behaved native protein.

187 The thermostability of A. fulgidus AlaDH was increased by mo

188 The changes in enzyme kinetics and thermostability of AAO in carrot puree could be related

189 Phe-79 or Tyr decreased the thermostability of actin and increased its nucleotide ex

190 not change, which may be related to greater thermostability of actin and myosin in FPH-8 as observed

191 The thermostability of an integral membrane protein (MP) in

192 The aim of this study was to improve the thermostability of Aspergillus sulphureus acidic xylanas

193 stigated the effects of glycosylation on the thermostability of Bacillus subtilis xylanase A (XynA) e

194 wo most successful strategies to improve the thermostability of beta(1)AR-m23 were an engineered salt

195 This arm substructure may underlie the thermostability of BstYI.

196 O-Mannosylation also increases the thermostability of CBM glycoforms up to 16 degrees C, an

197 Here we show that the average thermostability of class I molecules isolated from cells

198 the R789C mutation causing a decrease in the thermostability of collagen not only alters individual c

199 ea buckthorn extract revealed a satisfactory thermostability of compounds at high temperatures.

200 esearch was to study the enzyme kinetics and thermostability of endogenous ascorbic acid oxidase (AAO

201 biophysical analyses of the pH stability and thermostability of Fcepsilon and IgG1-Fc (Fcgamma).

202 is increasing interest in mechanisms for the thermostability of functional RNA molecules.

203 actions significantly contribute to the high thermostability of HDL in low-salt solutions.

204 Features important for the thermostability of htADH are suggested by a comparison w

205 Therefore, variation of thermostability of influenza virus isolates begins at th

206 s spectrometry), despite also decreasing the thermostability of Mb by >10 degrees C.

207 In an effort to increase the thermostability of Moloney Murine Leukemia Virus reverse

208 disulfide bond reduction does not affect the thermostability of monomer SOD1 as significantly as the

209 The thermostability of more than 25 phylogenetically dispers

210 l scanning calorimetry demonstrated that the thermostability of N26D/N131D/N139D SOD1 is lower than W

211 of these chimeric NDVs demonstrated that the thermostability of NDV was dependent on the origin of HN

212 should provide insight for understanding the thermostability of other thermophilic proteins.

213 nome organization contribute to the enhanced thermostability of PH75 relative to mesophilic filamento

214 ikewise proposed as contributors to the high thermostability of PH75 vis-a-vis fd, Pf1, and Pf3.

215 understand the structural basis for the high thermostability of phytase from Aspergillus fumigatus, i

216 etween strain replication efficiency and the thermostability of prion protein aggregates.

217 High thermostability of proteins is a prerequisite for their

218 he detected positive correlation between the thermostability of PrP(Sc) aggregates and disease transm

219 e the impact of DNA methylation marks on the thermostability of regulatory regions.

220 The thermostability of RNA molecules bred in the laboratory

221 The enhanced thermostability of the archaeal enzyme as compared to th

222 These results indicate that the thermostability of the bacterial collagen is dominated b

223 The thermostability of the biosensors after incubation at 60

224 A remarkable convergence between the thermostability of the capsid and those of the other vir

225 -dependent Raman spectra to characterize the thermostability of the Delta-domain secondary structure

226 Each HR mutation enhanced the thermostability of the endogenous 6HB, potentially allow

227 as been established that a difference in the thermostability of the ends of an siRNA duplex dictate w

228 mino acid substitutions without altering the thermostability of the entire collagen molecule.

229 r ceftazidime to purified PBP3 increases the thermostability of the enzyme significantly and is assoc

230 ced loop and the A80T mutation increased the thermostability of the enzyme.

231 --> Ser mutation is modeled by the relative thermostability of the Gly-X-Y triplet on the carboxy si

232 faces, and likely contributes to the extreme thermostability of the helical capsid.

233 as defined by NMR; (ii) it induces increased thermostability of the isolated N-terminal domain; (iii)

234 the redox-sensitive toggle that adjusts the thermostability of the linker region to the cell redox s

235 We speculate that low thermostability of the MARTX effector domains correlates

236 Alterations in the overall conformation and thermostability of the metal-oxidized protein were inves

237 Here, the effect of DNA and ATP on the thermostability of the Methanothermobacter thermautotrop

238 We demonstrate that thermostability of the model enzymes haloalkane dehaloge

239 tation increases, rather than decreases, the thermostability of the N-terminal domain of MDM2 in the

240 ents of PH75 that may be related to the high thermostability of the native virion assembly.

241 SOD1 zinc derivatives also showed increased thermostability of the protein due to zinc binding.

242 ramer were observed to drastically alter the thermostability of the protein.

243 from those of the native form, although the thermostability of the recombinant form was dramatically

244 o proteases, possibly explaining the unusual thermostability of the resulting protease-stalk complexe

245 Structure analysis suggests that the higher thermostability of the Rhodococcus enzyme may be attribu

246 he mutation I137M, previously shown to lower thermostability of the six-helix bundle, resulted in an

247 cal catalyst for the HER, combining the high thermostability of the surfaces and high catalytic activ

248 leading to dimer formation resulted in poor thermostability of the system, design success was facili

249 Studies of the chemical stability and thermostability of these enzymes thus become important.

250 The thermostability of these membranes is consistent with th

251 sed to account for the dramatically enhanced thermostability of this archaeal enzyme.

252 cally improve expression yields and in vitro thermostability of two therapeutically relevant antibodi

253 e femtomolar affinity, low off-rate and high thermostability of wild-type streptavidin.

254 nit that retained the affinity, off rate and thermostability of wild-type streptavidin.

255 Trends in the thermostability of yeast ADP/ATP carrier AAC2 and ovine

256 study of homologous proteins with differing thermostabilities offers an especially useful approach f

257 es (their dipole moment), confers structural thermostability on double-stranded sequences, and decrea

258 a broad activity range (pH 6-9) and moderate thermostability (optimum ca. 70 degrees C).

259 sulfide-crosslinked enzyme displays improved thermostability, particularly when combined with previou

260 The loop structure and its high thermostability preclude pseudoknot formation.

261 all avidins, shwanavidin also displays high thermostability properties.

262 increased amount of secondary structure and thermostability, reduction in exposed hydrophobic surfac

263 chrome P450 2C9 (CYP2C9), including elevated thermostability relative to that of CYP2C9, as well as a

264 ve primary defects in enzymatic activity and thermostability, respectively.

265 The pH dependence and thermostability reveal that the enzymes are highly adapt

266 To study the determinants of thermostability, several structural features including s

267 ified scaffolds determined on the basis of a thermostability shift assay, calorimetric binding data,

268 gns on proteins with experimentally measured thermostability show it is beneficial to compute both th

269 Global exchange experiments, supported by thermostability studies, demonstrate that fructose 1,6-b

270 ge rates, affinity for metarhodopsin II, and thermostability suggest that the K345L Galphai1 variant

271 The presence of subpopulations with high thermostability suggests that avian viruses can persist

272 c rod displays an exceedingly high intrinsic thermostability (T(m) approximately 85 degrees C), which

273 mately 90% alpha-helix), exhibits diminished thermostability (T(m)=40 degrees C; DeltaH(m)=27+/-2 kca

274 es suggest that porcine rotavirus has higher thermostability than human rotavirus.

275 tragonal superstructure exhibits much higher thermostability than its close-packed hexagonal counterp

276 articles with AASs show comparable or higher thermostability than that of AS.

277 , Ago loading of siRNAs is less sensitive to thermostability than that of their shRNA equivalents.

278 show that the Rhodococcus enzyme has higher thermostability than the naphthalene dioxygenase from Ps

279 s information have been shown to have higher thermostability than their biological counterparts.

280 nt of the remaining mutations showed reduced thermostability, the exceptions being the two later-onse

281 red electrophoretic mobility, relatively low thermostability, the presence of atypical disulfide bond

282 , increased mechanical strength and improved thermostability; this resilience should enable diverse a

283 ickly screen mutant structures for increased thermostability through optimization of the protein's el

284 ic technology to improve enzyme function and thermostability through sortase A-mediated crosslinking

285 uble protein expression levels (up 60%), and thermostability (Tm, 12 degrees C higher).

286 ein surface is an efficient method to confer thermostability to a mesophilic protein.

287 steines and secondary mutations that enhance thermostability to achieve a substantial gain in functio

288 shed by in vivo assembly at 70 degrees C and thermostability to at least 90 degrees C.

289 riations in one gene, allowing adaptation to thermostability to be studied in molecular detail as bac

290 re, random nonamer peptides conferred higher thermostability to HLA-B*4405 than to HLA-B*4402.

291 eening of mutant candidates likely to confer thermostability to mesophilic proteins through optimizat

292 ary structures, DtxR(E175K) gains additional thermostability upon activation with metal ions, which m

293 TCEP thermostability was equivalent in the presence of 5 or 5

294 the stability, experimental measurements of thermostability were done using differential scanning ca

295 Changes in thermostability were monitored using differential scanni

296 in, and demonstrated significantly increased thermostability while preserving protein activity.

297 the rational design of enzymes with enhanced thermostability while retaining full enzymatic activity.

298 type hAChE and exhibited 20 degrees C higher thermostability with no change in enzymatic properties o

299 N1-(2-aminoethyl)-hypoxanthine has a reduced thermostability with no particular pairing preference to

300 eavage-site mutant viruses displayed reduced thermostability, with double-cleavage-site mutants exhib