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1 capacity to incorporate iron, and were less thermostable.
2 are monomeric, photochromic, and remarkably thermostable.
3 ow-salt buffer at pH 5.7-7.7, HDL are highly thermostable.
4 SsMTAPII is extremely thermoactive and thermostable.
5 alized by atomic force microscopy) which are thermostable.
6 nase-C for fructose but is considerably more thermostable.
7 atalytic domain of TbCet1 is extraordinarily thermostable.
8 activity, has NADH-specific activity, and is thermostable.
9 nerated enzymes that were substantially less thermostable.
10 This activity was found to be remarkably thermostable.
11 gth F508del-CFTR was about 10 degrees C less thermostable.
12 mostability and the DGK peptide is the least thermostable.
15 pt, we have created a fusion consisting of a thermostable aldo-keto reductase, two alpha-helical leuc
18 ty predictably into a completely de novo and thermostable alpha-helical barrel, which comprises seven
23 and biophysical characterization of the most thermostable amylosucrase identified so far, the amylosu
24 y, we show CagY(rpt2) to comprise two highly thermostable and acid-stable alpha-helical structural mo
28 and fatty-acid glycerol esters are extremely thermostable and retain internal RNA and DNA oligonucleo
29 d a stabilizing salt bridge cluster, (ii) is thermostable and shows a folding mechanism similar to th
31 This enzyme was also found to be remarkably thermostable and was resistant to H2O2 at concentrations
32 n headpiece (HP67) harbors a highly helical, thermostable, and autonomously folding subdomain in the
33 rcSso7d-based binders are highly expressed, thermostable, and monomeric, suggesting that the hyperst
35 nstrate that the Pa-UDGa-Pa-PCNA1 complex is thermostable, and two hydrophobic amino acid residues on
36 icles that are monodispersed, biocompatible, thermostable, and water-soluble, allowing diverse membra
38 olfataricus P2 DNA polymerase IV (Dpo4) is a thermostable archaeal enzyme and a member of the error-p
39 Here the authors show a highly efficient and thermostable artificial enzyme that catalyzes a diverse
40 tion of a highly efficient, promiscuous, and thermostable artificial enzyme that catalyzes a diverse
41 plex II overproduced in Escherichia coli are thermostable as isolated, but moderately sensitive to re
42 des 1 to 754 were dimeric, with a linkage as thermostable as that between dimers of intact genomic RN
44 The 1D and 2D RIDC3 assemblies are highly thermostable as well, remaining intact at up to approxim
47 showed that folding of the smaller and more thermostable Azoarcus ribozyme is well described by two
51 expression of fusions of a coding gene for a thermostable beta-galactosidase to a number of heat shoc
54 neous catalysis, yielding high-surface area, thermostable biochemical structures readily adaptable to
55 hich retains actin-binding activity, is more thermostable but, interestingly, does not exhibit the pa
63 nucleases and proteases, this reconstituted thermostable cell-free protein synthesis system may enab
65 . fusca is a model organism for the study of thermostable cellulases and is a major degrader of plant
67 nto a set of general rules for the design of thermostable CHA circuits with high signals and low nois
68 ication processes and demonstrating superior thermostable characteristics and immunogenicity, this st
69 Biochemical and X-ray scattering studies of thermostable CheA from Thermotoga maritima determine tha
72 eries, both cell types express predominantly thermostable class I and are critically dependent on TAP
74 ficant thermal resilience assessed as a high thermostable "coked" fraction in thermal carbon analysis
76 of free DNA and a second corresponding to a thermostable complex with a transition temperature, T(m(
77 ranslated trespin forms detergent-stable and thermostable complexes with plasmin and elastase but not
81 those in natural collagen and are remarkably thermostable, despite the absence of higher-order associ
84 ar beta-hemolysin phenotype, we surmise that thermostable direct hemolysin (TDH) plays a limited role
86 of the template, oligonucleotides, salt, and thermostable DNA ligase concentrations increased the spe
89 arious length initiating primers, with three thermostable DNA ligases: Thermus thermophilus (Tth), Th
90 hundreds to thousands of nucleotides by the thermostable DNA polymerase from Thermococcus litoralis
91 ion, we attempted to evolve a high-fidelity, thermostable DNA polymerase to use RNA templates efficie
92 s method to construct genes encoding a small thermostable DNA polymerase, a highly repetitive DNA seq
93 s, we previously reported the evolution of a thermostable DNA polymerase, SFM4-3, that more efficient
96 ately, the relatively high fidelities of the thermostable DNA polymerases commonly used for PCR resul
99 fined specificities, Ptr1 and Ptr2 provide a thermostable DNA-binding platform for analysis of effect
100 II mutants suggest that, in contrast to the thermostable domains, the thermolabile domains are able
101 ification and characterization of five novel thermostable Dpo4-like enzymes from Acidianus infernus,
103 the consequences of a method that utilizes a thermostable duplex-specific nuclease for reducing the h
104 ller version of N35CCG-N13), and the minimal thermostable ectodomain core of gp41 in its six-helix bu
105 il is stabilized both by fusion to a minimal thermostable ectodomain of gp41 and by engineered inters
107 f gp41 fused in helical phase to the minimal thermostable ectodomain of gp41, while the latter compri
111 ped a mutation scanning method that combines thermostable EndonucleaseV (Endo V) and DNA ligase.
112 (C)-like domains of TprC and TprI are highly thermostable, endowing the full-length proteins with imp
113 omologue of ht-ADH, ps-A25Y, leads to a more thermostable enzyme and a change in the rate-determining
114 capable of enhancing the folding yield of a thermostable enzyme at elevated temperature (55 degrees
115 rmus aquaticus DNA polymerase I (Taq Pol), a thermostable enzyme commonly used for DNA amplification
121 s of Dr-OPH and have found it to be a highly thermostable enzyme, remaining active after 3 h of incub
124 f between enzyme stability and activity with thermostable enzymes being less efficient catalysts that
128 g is a promising source of new organisms and thermostable enzymes that may be helpful in environmenta
129 ission maximum of 546 nm, providing a set of thermostable enzymes whose bioluminescence maxima were s
130 e from representing a potential reservoir of thermostable enzymes, thermophilic fungi are amenable to
137 e on the isolation and characterization of a thermostable F420: NADPH oxidoreductase (Tfu-FNO) from T
138 , Lam16A, from T. maritima comprise a highly thermostable family 4 CBM that binds beta-1,3- and beta-
140 study, we first created a mutant (mtfl) of a thermostable firefly luciferase (tfl) bearing the peroxi
141 ngle soluble fusion proteins consisting of a thermostable firefly luciferase variant that catalyze ye
145 be grouped into either a thermosensitive or thermostable fraction for both egg- and MDCK-grown virus
146 the proteolytic activity, in particular the thermostable fraction, should be considered as a target
148 for the first time, we engineered CHA to be thermostable from 37 to 60 degrees C and in consequence
150 h an increased lifetime in human plasma, the thermostable GeoCas9 provides the foundation for improve
152 ratures was developed by utilizing a "wired" thermostable glucose-6-phosphate dehydrogenase (tG6PDH)
153 ill allow high-throughput screening of other thermostable glucose-producing enzymes, including those
156 regulates transcription of mgfp5-ER encoding thermostable green fluorescent protein (GFP) or with a s
157 dth of cellular ligands for IFIT5 by using a thermostable group II intron reverse transcriptase for R
159 ve base methylations and a highly processive thermostable group II intron reverse transcriptase to ov
160 odes DMS modifications as mismatches using a thermostable group II intron reverse transcriptase.
161 loits a novel template-switching activity of thermostable group II intron reverse transcriptases (TGI
162 ermined a crystal structure of a full-length thermostable group II intron RT in complex with an RNA t
164 g a premature termination codon in ORF1 or a thermostable hairpin in the inter-ORF spacer reduces ORF
165 backbone conformational fluctuations in the thermostable helical subdomain, HP36, of the F-actin-bin
166 functional in isolation, but together form a thermostable heterooligomeric Rca that can use both alph
167 ophilus (BstHPr) was established as the most thermostable homologue and also the homologue with highe
168 we demonstrate that lipids render ISVPs less thermostable in a virus concentration-dependent manner.
169 esting that the GP64 trimers were relatively thermostable in the absence of the intermolecular disulf
170 alian guanylyltransferases are intrinsically thermostable in vitro and they are unaffected by Cet1(23
171 ature isothermal amplification method with a thermostable invertase, we can directly transduce Middle
173 in the target gene and were ligated using a thermostable ligase enzyme only when the genomic DNA car
174 er require tedious purification steps or use thermostable ligases that can generate side products dur
176 ilus P1 lipase provides a template for other thermostable lipases, and offers insight into mechanisms
179 and -2, respectively) were induced to form a thermostable (melting temperature, approximately 90 degr
181 (WT) enzyme, several active site mutants, a thermostable mutant (12X-PTDH), and a thermostable mutan
182 ning with a previously reported red-emitting thermostable mutant and using mutagenesis techniques, we
184 nts, a thermostable mutant (12X-PTDH), and a thermostable mutant with dual cofactor specificity (NADP
185 es, including ISVPs of a previously reported thermostable mutant, provided further insights into the
188 of states profiles are similar overall, both thermostable mutants show increased populations of the v
190 sient transfection and the identification of thermostable mutants using a thermostability assay that
191 Incubation of excess benzyl alcohol with thermostable N-benzyl-N-nitrosoamides at ambient tempera
192 aryotic thermophiles as source for purifying thermostable native macromolecular complexes with an emp
193 t of thermostability, and the HN gene from a thermostable NDV could be engineered into a thermolabile
196 have cloned, expressed, and characterized a thermostable NOS homolog from the thermophilic bacterium
198 The ligand-free receptor, solubilized in thermostable octyl glucoside micelles, exhibited a coope
199 ) dependent glucose dehydrogenase (GDH) is a thermostable, oxygen insensitive redox enzyme used in bi
200 rge aromatic network found in the only other thermostable P450, CYP119, thought to contribute to ther
201 I), have been analyzed in the reaction of a thermostable P450, CYP119, with meta-chloroperoxybenzoic
202 ictions, we constructed a family of 44 novel thermostable P450s with half-lives of inactivation at 57
203 ility between the A4V mutant protein and its thermostable parent (HSOD-AS), in which free cysteine re
204 activation temperatures higher than the most thermostable parent CBH II, from Humicola insolens, and
211 led triggers in less than 10 min by means of thermostable polymerase and nicking endonuclease activit
214 imal to best support PCR amplification using thermostable polymerases of a six-letter alphabet that i
215 erase-evolution system and its use to evolve thermostable polymerases that efficiently interconvert C
217 as accessible to enzymatic removal using the thermostable protease isolated from Pyrococcus furiosus.
221 icates disulfide bonding in stabilizing many thermostable proteins and points to novel chemical envir
222 ein mass spectrometric methods to explore if thermostable proteins could be used to identify rendered
226 n more thermally altered samples, so-called 'thermostable' proteins such as osteocalcin which has bee
227 s well as four other cocrystal structures of thermostable PTDH and its variants with different ligand
229 promise to be a source of highly functional, thermostable Rca candidates for thermal fortification of
230 an acid metabolism (CAM) plants must possess thermostable Rca to support Calvin-Benson-Bassham cycle
231 es C exposure, the transgenic lines with the thermostable RCA1 variants exhibited higher photosynthet
234 Using structural information as a guide, a thermostable recombinant chaperonin subunit was modified
235 resent studies on the production of a set of thermostable red- and green-emitting luciferase mutants
236 electrocatalytically reoxidized to NAD by a thermostable redox mediator, osmium (1,10-phenanthroline
237 modification of PCR-based methods, employing thermostable restriction enzymes to block the synthesis
238 dapters for next-generation sequencing using thermostable RNA ligase from Methanobacterium thermoauto
241 neered a thermostable xylanase (XynB) with a thermostable self-splicing bacterial intein to control t
242 The enzyme was found to be comparatively thermostable showing maximum activity at 60 degrees C fo
243 as good solvent and pH stability and is very thermostable, showing no loss of activity after incubati
245 0977 in E. coli resulted in high levels of a thermostable single-stranded DNase activity with propert
246 The engineered biocatalyst was shown to be a thermostable, solvent tolerant, and effective cofactor-r
249 ructure of a high fidelity polymerase from a thermostable strain of Bacillus stearothermophilus (Baci
251 ding domain (TBD) into the distantly related thermostable Taq DNA polymerase at an analogous position
254 o engineer active P450 enzymes that are more thermostable than any previously made by chimeragenesis,
257 Ruminococcus lactaris is significantly more thermostable than CdiA-CT(EC536), and its intrinsic tRNA
259 beta2 CP assembled at 105 degrees C was more thermostable than either the alpha+beta1+beta2 version a
263 a mutant receptor that is 31 degrees C more thermostable than the wild-type receptor in dodecylmalto
266 roteins from thermophiles are generally more thermostable than their mesophilic homologs, but little
267 uses bearing the TS09-C HN protein were more thermostable than those containing LaSota HN protein.
270 47C, which in the absence of calcium is more thermostable than wild type CjXyn10A bound to the divale
272 s conducted at seven conserved motifs of the thermostable Thermotoga maritima endonuclease V to probe
273 on-PCR (qRT-PCR) assay using the recombinant thermostable Thermus thermophilus (rTth) enzyme was deve
274 NA structural motifs for the construction of thermostable three-dimensional nano-architectures that d
276 97M SOSIP.664 trimers as being appropriately thermostable (Tm of 63.4 degrees C and 62.7 degrees C, r
278 models the final fusion state thought to be thermostable trimers with six-helix bundle structure in
279 iruses by exchanging viral genes between the thermostable TS09-C strain and thermolabile LaSota strai
282 rmance of HDA, we have cloned and purified a thermostable UvrD helicase (Tte-UvrD) and the mutL homol
283 he keys to success were the development of a thermostable vaccine and the application of participator
285 ent the cocrystal structure of an engineered thermostable variant of PTDH bound to NAD(+) (1.7 A reso
289 Room-temperature activities of the evolved thermostable variants range from 3.5 times greater to 4.
291 -nitrobenzyl esterase (484 residues) and two thermostable variants that were generated by laboratory
292 ides and biomolecules in the design of their thermostable variants where the shielding mechanism can
294 Chimeras bearing the HN protein derived from thermostable virus exhibited a thermostable phenotype, a
296 ernix bacilliform virus 1) is among the most thermostable viruses known; it infects a hyperthermophil
298 Moreover, crosslinked SCRII was much more thermostable with its denaturation temperature (Tm) incr
300 To overcome this problem, we engineered a thermostable xylanase (XynB) with a thermostable self-sp
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