ライフサイエンスコーパス: thiolase

1 ogen biosynthesis and peroxisomal 3-ketoacyl thiolase.

2 shed by deleting the first 16 amino acids of thiolase.

3 and reduction of p46Shc expression activates thiolase.

4 ent of the peroxisomal matrix, e-oxoacyl-CoA thiolase.

5 ted whether Peb1p interacts with the PTS2 of thiolase.

6 ifunctional enzyme, and 3-oxoacyl-coenzyme A thiolase.

7 -CoA hydratase and long-chain 3-ketoacyl-CoA thiolase.

8 ith the mango, cucumber, and rat peroxisomal thiolases.

9 thylglutaryl-CoA synthases, and biosynthetic thiolases.

10 with amino acid sequence homology to type II thiolases.

11 2 acx2-1 double mutants and the ketoacyl-CoA thiolase-2 (kat2) mutant exhibit a sucrose-independent g

12 l coenzyme A (acetyl-CoA) acetyltransferase (thiolase), 3-hydroxybutyryl-CoA dehydrogenase, and acyl-

13 te could be partially restored by expressing thiolase (a PTS2-containing enzyme) fused to the PTS1.

14 studies showed colocalization of HsPMP20 and thiolase, a bona fide peroxisomal protein.

15 Acetoacetyl CoA thiolase (AACT, EC 2.3.1.9) catalyzes the condensation o

16 is the lipid oxidation enzyme 3-ketoacylCoA thiolase ACAA2, to which p46Shc binds directly and with

17 g the cysteine, considered to be part of the thiolase active site, the kiwifruit protein shows approx

18 AcAc-CoA thiolase activities also paralleled HMG-CoA reductase an

19 cytosolic acetoacetyl coenzyme A (AcAc-CoA) thiolase activities.

20 hromatography was shown to have both ACT and thiolase activities.

21 y to regulatory stimuli than acetoacetyl-CoA thiolase activity but usually less than HMG-CoA reductas

22 es its expression and eliminates most of the thiolase activity in seedlings.

23 nificantly lower (k(cat) 3 min(-1)) than the thiolase activity of FadA (k(cat) 2170 min(-1)).

24 uggest p46Shc to be a negative mitochondrial thiolase activity regulator, and reduction of p46Shc exp

25 gher lipid oxidation capacity, and increased thiolase activity.

26 ocated downstream from yfcY, a gene encoding thiolase activity.

27 greater than 99% decrease in k(cat) for the thiolase activity.

28 indicating that the mutant lacks long-chain thiolase activity.

29 f a protein that directly binds and controls thiolase activity.

30 Mature thiolase alone, lacking the PTS2 signal, was not importe

31 sely affected the activity of 3-ketoacyl-CoA thiolase although not enough to become rate-limiting.

32 structural homology to aldolases, acts as a thiolase, an activity previously undescribed for this fa

33 e mevalonate pathway, acetoacetyl-coenzyme A thiolase and 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-

34 Similarly, induction of hepatic thiolase and bifunctional enzyme also required expressio

35 e product catalyzed both the acetoacetyl-CoA thiolase and HMG-CoA reductase reactions.

36 The catalytic properties of 3-ketoacyl-CoA thiolase and l-3-hydroxyacyl-CoA dehydrogenase of the mu

37 Under conditions at which acetoacetyl-CoA thiolase and long-chain thiolase were completely inactiv

38 ith ACP has not previously been observed for thiolases and in the case of the S. collinus FadA is sig

39 roxisomes or for the import of 3-oxoacyl-CoA thiolase (and at least two other peroxisomal matrix prot

40 cyl-CoA oxidase, L-bifunctional protein, and thiolase, and (b) a second noninducible pathway catalyzi

41 Moreover, the stabilized ICL, thiolase, and an ICL-GFP reporter remained peroxisome as

42 tor-activated receptor alpha (PPARalpha) and thiolase, and an up-regulation of PPARgamma, a positive

43 hase, acyl-CoA dehydrogenase, 3-ketoacyl-CoA thiolase, and many proteins involved in chaperone activi

44 At this stage, the basal mRNA levels of HD, thiolase, and other peroxisome proliferator-induced targ

45 me A (CoA), acyl CoA synthetase, oxoacyl CoA thiolase, and ubiquitin also were underexpressed in NASH

46 drial aldehyde dehydrogenase, 3-ketoacyl-CoA thiolases, and adenosine triphosphate synthase.

47 e and reduced accumulation of 3-ketoacyl-CoA thiolase, another PTS2-containing protein; both defects

48 e 2 peroxisomal targeting sequence (PTS2) of thiolase are defective, whereas the biogenesis of protei

49 n chains and a coenzyme moiety-unusual for a thiolase-are unknown.

50 lso related to the E. coli and mitochondrial thiolases, as well as to the acetoacetyl-CoA thiolases o

51 In the thiolase assay kinetic analyses revealed similar K(m) va

52 oxisomal Arabidopsis thaliana 3-ketoacyl-CoA thiolase (AtKAT), an enzyme of fatty acid beta-oxidation

53 Our results expand the utility of thiolase-based pathways and provide biosynthetic access

54 PTS2 protein imports increased the level of thiolase bound and imported into the organelles.

55 Although the basics of thiolase chemistry are precedented, the mechanism by whi

56 nase deficiency, medium-chain 3-ketoacyl-CoA thiolase deficiency, 3-hydroxy-3-methylglutaryl-CoA synt

57 In competition experiments, mature thiolase did not affect the import of a PTS1 protein, bu

58 The import of the PTS2 protein thiolase differed from PTS1 protein import in several wa

59 Thiolase differs from these other peroxisomal proteins i

60 onate is virtually nil because acetoacyl-CoA thiolase does not favor the formation of beta-ketopentan

61 mature protein competes with the full-length thiolase during assembly of an import complex at the sur

62 t was missing the peroxisomal 3-ketoacyl-CoA thiolase encoded by the PEROXISOME DEFECTIVE1 (PED1/At2g

63 in ciliated neurons and by a 3-ketoacyl-coA thiolase (encoded by kat-1) that acts in fat storage tis

64 The catalytic properties of 3-ketoacyl-CoA thiolase, enoyl-CoA hydratase, and delta 3-cis-delta 2-t

65 In this regard, synthetic pathways based on thiolase enzymes to catalyze the initial carbon-carbon b

66 the previously characterized CoA-ligase and thiolase enzymes, provides evidence that the whole pathw

67 Features distinguish it from members of the thiolase family, suggesting that it carries out a relate

68 , like other KAS-II enzymes, mtKasB adopts a thiolase fold but contains unique structural features in

69 om Enterococcus is a member of the family of thiolase fold enzymes and, while similar to the recently

70 leaves, as does PED1 (encoding a 3-keto-acyl-thiolase for beta-oxidation).

71 n fluorescent protein chimera of peroxisomal thiolase, formation of peroxisomes, and peroxisome funct

72 oleate-induced PTS2-dependent import of the thiolase Fox3p into peroxisomes is conducted by the solu

73 SNO-CoA reductase, protects acetoacetyl-CoA thiolase from inhibitory S-nitrosylation and thereby aff

74 ivity is consistent with a beta-ketoacyl-CoA thiolase function in cholesterol beta-oxidation that is

75 ndogenous clostridial promoters: that of the thiolase gene (thlP) and that for the clostridial transc

76 -acetyl CoA, and KAT2 appears to be the only thiolase gene expressed at significant levels during ger

77 t loss-of-function mutations of the ketoacyl thiolase gene kat-1 result in an increased accumulation

78 An inhibitor of long-chain 3-ketoacyl-CoA thiolase has been developed as a tool for probing the co

79 se/3-hydroxyacyl-CoA dehydrogenase (HD), and thiolase, has been examined in mice by disrupting ACOX g

80 y using HMG-CoA synthase and acetoacetyl-CoA thiolase/HMG-CoA reductase from E. faecalis.

81 Kinetic studies of acetoacetyl-CoA thiolase implicated a ping-pong mechanism.

82 Thiolase import was slower than typical PTS1 protein imp

83 irming that the PTS2 signal is necessary for thiolase import.

84 sing p46Shc stimulates enzymatic activity of thiolase in vitro Thus, we suggest p46Shc to be a negati

85 Pex7p is functional, resulting in import of thiolase into peroxisomes and improved growth of the yea

86 at is selectively defective in the import of thiolase into peroxisomes but has a normal ability to pa

87 The activity of 3-ketoacyl-CoA thiolase involved in mitochondrial beta-oxidation of fat

88 Peb1p is found in peroxisomes whether thiolase is expressed or not.

89 t the peroxisomal packaging of PTS2 targeted thiolase is lacking.

90 Thiolase is the last enzyme of the mitochondrial fatty a

91 The subunit, a typical thiolase, is a combination of two similar alpha/beta dom

92 mosome 2 (KAT2), which encodes a peroxisomal thiolase, is activated in early seedling growth.

93 3-ketoacyl-CoA thiolase (KAT) (EC: 2.3.1.16) catalyses a key step in fa

94 Reduced fertilization was also observed in thiolase (kat2-1) and peroxisomal acyl-Coenzyme A synthe

95 Of the two major 3-ketoacyl coenzyme A thiolases, KAT2 plays the primary role in BA synthesis.

96 cation and biochemical characterization of a thiolase-ketoreductase pair involved in production of br

97 The fusion protein was expressed in a thiolase knockout strain.

98 id residues were fused in front of truncated thiolase lacking the NH2-terminal 16-amino acid PTS2.

99 lectively inhibits long-chain 3-ketoacyl CoA thiolase (LC 3-KAT), thereby reducing fatty acid oxidati

100 thiolases, as well as to the acetoacetyl-CoA thiolases of prokaryotes.

101 The thiolase-p46Shc connection shown here in vitro and in or

102 Finally, a thiolase-Peb1p complex was isolated by immunoprecipitati

103 ies of the mutant strains indicated that the thiolase reaction is the limiting step in PHB biosynthes

104 were completely inactivated, 3-ketoacyl-CoA thiolase retained some activity.

105 lity of residues within the motif, rat liver thiolase (rthio) and various chimeric chloramphenicol ac

106 carrier protein synthases are members of the thiolase superfamily and are key regulators of bacterial

107 Degradative thiolases, which are part of the thiolase superfamily and naturally function in the beta-

108 that is critical for this process is OleA, a thiolase superfamily enzyme that condenses two fatty acy

109 OleA is a thiolase superfamily enzyme that has been shown to catal

110 In thiolase superfamily enzymes, catalysis is achieved via

111 udies of the HMG-CoA synthase members of the thiolase superfamily have shown that the catalytic loop

112 OleA is the first characterized thiolase superfamily member that has two long-chain alky

113 rmation can be catalysed by enzymes from the thiolase superfamily, including beta-ketoacyl-acyl-carri

114 etabeta fold, which is characteristic of the thiolase superfamily.

115 substrates of this particular member of the thiolase superfamily.

116 sidue five, a change that is known to reduce thiolase targeting in vivo.

117 ha-branched products even when paired with a thiolase that highly prefers unbranched linear products.

118 erved a strong interaction between Peb1p and thiolase that was abolished by deleting the first 16 ami

119 rease the amount of radiolabeled full-length thiolase that was imported.

120 546) gene, annotated as a lipid-metabolizing thiolase, the expression of which is upregulated by chol

121 ed genes including peroxisomal 3-oxoacyl-CoA thiolase (THIO), peroxisomal enoyl-CoA hydratase/3-hydro

122 ofluorescence, localization of 3-oxoacyl-CoA thiolase to peroxisomes was unchanged whether Pal1 was p

123 Thiolases typically utilize a ping-pong mechanism center

124 ld lower and the Km for the substrate of the thiolase was 6-fold higher.

125 de consisting of the first 16 amino acids of thiolase was sufficient for the affinity binding of Peb1

126 Thiolase was thought previously only to be regulated by

127 hich acetoacetyl-CoA thiolase and long-chain thiolase were completely inactivated, 3-ketoacyl-CoA thi

128 ICL and thiolase were mislocalized to the cytosol but only ICL w

129 ICL, MLS, and the beta-oxidation enzyme thiolase were stabilized in the pex4-1 pex22-1 double mu

130 Of all beta-oxidation enzymes, only thiolases were inactivated by the inhibitor.

131 on enzymes (CYP4A3, bifunctional enzyme, and thiolase) were observed in the livers of HNF1alpha-null

132 dehydrogenase, and long-chain 3-ketoacyl-CoA thiolase, were determined with substrates having acyl ch

133 ty acyl-CoA oxidase, bifunctional enzyme, or thiolase, which accompanies peroxisome proliferation in

134 Degradative thiolases, which are part of the thiolase superfamily an

135 related to the peroxisomal beta-ketoacyl-CoA thiolases, which catalyze the CoA-dependent degradative