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1 regression plot of input copy number versus threshold cycle by using HIV-1 RNA transcripts at copy n
4 RNA qPCR exhibited a significant decrease in threshold cycle (C(T)) time values (C(T) control - C(T)
7 tive by the CDC rRT-PCR by virtue of showing threshold cycle (C(T)) values only with universal InfA a
8 t in clinical samples and EQA samples with a threshold cycle (CT ) value of <30 were detected correct
9 ve quantities of the transgene by taking the threshold cycle (Ct) of both the transgene and an intern
10 se polymerase chain reaction (qRT-PCR)-based threshold cycle (Ct) value and the presence of infectiou
12 orming units, and respiratory specimens with threshold cycle (CT) values of <34 typically produced go
13 As expected, WGA products with low (<16.0) threshold cycle (CT) values yielded mostly Cryptosporidi
14 ime polymerase chain reaction (RT-qPCR) uses threshold cycles (Ct values) for measuring relative gene
15 y Prodesse ProFlu+ PCR (a mean real-time PCR threshold cycle [CT] value of 31.9 +/- 2.0), which inclu
16 le data, the standard curve constructed from threshold cycle data had a multiple R2 value of 1.00 and
17 actin gene, the coefficient of variation for threshold cycle data was 1.6%, whereas it increased to 1
18 d curves obtained from band densitometry and threshold cycle data, the standard curve constructed fro
23 tarting copy numbers were amplified, and the threshold cycle showed an increase for decreasing amount
25 c children, we defined a cutoff point in the threshold cycle value (26.7) that predicts rotavirus-att
26 calculated for each pathogen by plotting the threshold cycle value against the bacterial number (log
27 e Method, for analysis of qPCR data based on threshold cycle values (C q ) and efficiencies of reacti
28 reaction (PCR) amplification showed similar threshold cycle values as those obtained from a commerci
30 ot detect HAdV with either low viral burden (threshold cycle values of >30) or nonrespiratory species
31 eal-time PCR (linear correlation between the threshold cycle versus log DNA copy number, >0.982).
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