ライフサイエンスコーパス: tissue culture

1 antagonizing replication of some viruses in tissue culture.

2 embryogenesis or experimentally in in vitro tissue culture.

3 C, were further characterized against HCV in tissue culture.

4 y sequencing when the virus is propagated in tissue culture.

5 of recent human H3N2 viruses upon passage in tissue culture.

6 zed transfection of C. parvum sporozoites in tissue culture.

7 28 aids in promoting experimental latency in tissue culture.

8 luorene 11 as a potent DNA cleavage agent in tissue culture.

9 expression resulting in enhanced shooting in tissue culture.

10 d not convert back to tachyzoites readily in tissue culture.

11 gh these two cell lines migrate similarly on tissue culture.

12 killing induced by E4orf4 in normal cells in tissue culture.

13 ecificity compared to other methods, such as tissue culture.

14 ected cells and neutralized VZV infection in tissue culture.

15 BRAFV600E/PTENNull melanomas in vivo and in tissue culture.

16 Most transgenic crops are produced through tissue culture.

17 n antigen expressed by malignant B cells, in tissue culture.

18 ced into 523-3 can be efficiently induced by tissue culture.

19 cytoskeletal structures in cells growing in tissue culture.

20 ormal plant tissue but can be reactivated by tissue culture.

21 pergillus fumigatus and evaluated ex vivo in tissue culture.

22 om a single transformed plant regenerated in tissue culture.

23 onocytogenes invasion of epithelial cells in tissue culture.

24 venting the outgrowth of aneuploid clones in tissue culture.

25 as historically been refractory to growth in tissue culture.

26 rical (Mayinga) EBOV strains was observed in tissue culture.

27 r and to prevent the replication of HIV-1 in tissue culture.

28 ood culture bottle system for periprosthetic tissue culture.

29 thelial cell lines were grown in vivo versus tissue cultures.

30 ion system for the quantitation of thiols in tissue cultures.

31 ll-cell adhesion, and formation of crypts in tissue cultures.

32 mix-ups and contamination in human cell and tissue cultures.

33 nce that the spontaneous mutation T544I is a tissue culture adaptation in certain cell lines and that

34 Tissue culture adaptation of cytomegaloviruses rapidly s

35 a single passage, consistent with it being a tissue culture adaptation.

36 the interaction between alphavbeta6 and two tissue culture adapted FMDV strains by cryo-electron mic

37 Infection can also occur in tissue culture adapted virus in the absence of integrin

38 d to the wild-type (WT) PoSaV Cowden strain, tissue culture-adapted (TC) PoSaV has two conserved amin

39 highly virulent U.S. PEDV strain PC21A, the tissue culture-adapted PC177 (TC-PC177) contains a 197-a

40 The tissue culture-adapted porcine sapovirus Cowden strain i

41 n gnotobiotic pigs than those induced by the tissue culture-adapted strain.

42 Because the wild-type MERS-CoV contains a tissue culture-adapted T1015N mutation in the S glycopro

43 BTV serotypes are incorporated into a common tissue-culture-adapted backbone.

44 exogenous fluorescent reporter gene in both tissue culture and a live animal model, as well as light

45 bited growth of lung adenocarcinoma cells in tissue culture and abolished growth of lung adenocarcino

46 In tissue culture and animal models, sex hormones regulate

47 at lyses single adherent cells from standard tissue culture and captures the contents to perform sing

48 A in trans rescued KLKI MHV68 replication in tissue culture and enabled detection of KLKI MHV68 react

49 bility than CD24(-)CD29(-)/CD49f(-) cells in tissue culture and enhanced metastatic potential in allo

50 gy pathway caused more rapid viral spread in tissue culture and greater pathogenicity in mice.

51 G-to-A mutations in the HIV genome, both in tissue culture and in HIV positive patients undergoing K

52 ing a recombinant virus-expression system in tissue culture and in mice, we demonstrate that gentamic

53 e resulting mutant viruses were evaluated in tissue culture and in mice.

54 Both in tissue culture and in mouse models, these imaging biocon

55 In tissue culture and in the liver colonies derived from ca

56 Combined tissue culture and in vivo data support that environment

57 Both tissue culture and in vivo experiments in mice demonstra

58 (miRNAs) have been widely used in mammalian tissue culture and model organisms to selectively silenc

59 ifested as a decrease in infectivity in both tissue culture and murine infection models.

60 reduced glycoprotein expression to growth in tissue culture and pathogenesis in BALB/c mice.

61 ll, of the capsid protein is rapidly shed in tissue culture and primary target cells, independent of

62 lacking a rigid cell wall and is observed in tissue culture and single-celled organisms, as well as i

63 with the drug 4-hydroxy-tamoxifen (4-OHT) in tissue culture and transgenic zebrafish.

64 Intraoperative fluid and tissue cultures and pre- and postoperative synovial flui

65 go by passaging wild-type JEV strain SA14 in tissue cultures and rodents, with intermittent tissue cu

66 e peptide cleavage sites also varied between tissue-cultured and primary cells, with 5- and 8-residue

67 an enhanced ability to scatter and invade in tissue culture as was observed in mutant p53 cells.

68 lly active and stimulates ErbB3 signaling in tissue culture assays.

69 a derivative strain passaged extensively in tissue culture (BTV8H) in in vitro and in vivo studies.

70 netics similar to that of wild-type virus in tissue culture but caused a dramatically more aggressive

71 imeric viruses were attenuated for growth in tissue culture but could be propagated to high titers wi

72 Oncolytic viruses obliterate tumor cells in tissue culture but not against the same tumors in vivo.

73 ukemia virus (MLV) can efficiently spread in tissue cultures by polarizing assembly to virological sy

74 Virus growth in tissue culture can be attenuated by introduction of muta

75 cid-derived ammonia, most mammalian cells in tissue culture cannot proliferate or even survive in an

76 embryonic brain tissue, as well as in human tissue culture cell lines using RNA in situ hybridizatio

77 eltaSHDeltaV indicated that it was stable in tissue culture cell lines.

78 enzymatic behavior between primary cells and tissue-cultured cell lines.

79 Tissue culture cells and Caenorhabditis elegans embryos

80 We show that 'EC-tagging' occurs in tissue culture cells and Drosophila engineered to expres

81 inase A (PKA) activity stabilizes PER, in S2 tissue culture cells and in fly circadian neurons.

82 difficile toxin TcdA activates p38 kinase in tissue culture cells and mouse ilium, resulting in inter

83 thodology to measure microtubule assembly in tissue culture cells and Xenopus egg extracts using two-

84 spindle assembly, we combined experiments in tissue culture cells and Xenopus laevis egg extracts wit

85 Similar rings are observed in Xenopus tissue culture cells at a lower frequency but are enrich

86 cells and had less of a cytopathic effect on tissue culture cells but caused severe disease in mice.

87 ways compromises bipolar spindle assembly in tissue culture cells but not in X. laevis egg extracts.

88 Human tissue culture cells have long been a staple of molecula

89 glycoproteins and protected mucus-producing tissue culture cells in vitro.

90 ction, and when expressed alone in mammalian tissue culture cells it induces protein phosphatase 2A (

91 outine procedure used to dissociate adherent tissue culture cells prior to RNA extraction.

92 ne dyes malachite green and brilliant green, tissue culture cells recruit beta-arrestins to clathrin

93 In vitro, depletion of FL2 from mammalian tissue culture cells results in a more than twofold incr

94 However, our experiments in tissue culture cells show that viral RNA synthesis and n

95 In vitro studies of tissue culture cells with and without surface mucus demo

96 filamentous structures, or rodlets, in human tissue culture cells, after gene transfer to adult mice,

97 vivo, both in Xenopus embryos and mammalian tissue culture cells, also impacts nuclear size.

98 y(A) tail shortening and mRNA degradation in tissue culture cells, and we detect a reduced number of

99 Therefore, in tissue culture cells, input and progeny genomes are not

100 racts with several proteins in Drosophila S2 tissue culture cells, the majority of which are splicing

101 Using human tissue culture cells, we found that hEndoU localizes to

102 Using semi-intact tissue culture cells, we performed a polysome solubiliza

103 croscopy (STORM) to Xenopus egg extracts and tissue culture cells, we report various distribution pat

104 d exchange at the frequency of 5% in primary tissue culture cells, whereas higher levels were seen in

105 tore the mutant's ability to form plaques in tissue culture cells.

106 tor), which inhibits several HECT ligases in tissue culture cells.

107 bserved enhancer activity of this element in tissue culture cells.

108 hen ectopically expressed in jump muscles or tissue culture cells.

109 swarming motility, and form small plaques in tissue culture cells.

110 otein in the control of centrosome number in tissue culture cells.

111 n autonomously in single-cell eukaryotes and tissue culture cells; however, within the context of a m

112 ntry and that of infectious EBOV and MARV in tissue cultured cells.

113 bit cell proliferation when overexpressed in tissue-culture cells, and they can function as tumor sup

114 n activator Chorion factor 2 in flies and in tissue-culture cells.

115 thereby negatively regulate YAP1 activity in tissue-cultured cells.

116 an be overcome by adaptation of the virus in tissue-cultured cells.

117 e have been able to overcome these blocks in tissue-cultured cells.

118 l currents of fetal mouse AChRs expressed in tissue-cultured cells.

119 stabilizes microtubules both in vitro and in tissue-cultured cells.

120 can be precisely controlled in standard cell/tissue culture conditions and in vivo.

121 s to inhibition of cell proliferation, under tissue culture conditions as well as in vivo.

122 We find that under standard serum-containing tissue culture conditions, ATSP-7041 achieves intracellu

123 f a spontaneous mutation (T544I) specific to tissue culture conditions, suggesting that it has no rol

124 rosis compared to cells cultured in standard tissue culture conditions.

125 strain with no discernible growth defect in tissue culture, contained a 2,158-bp deletion in open re

126 oss of Karma methylation and of small RNA in tissue culture contributes to the origin of mantled, whi

127 plate-reader enzyme immunoassay, and direct tissue culture cytotoxicity.

128 ts to produce clinically relevant numbers of tissue-culture-derived platelets for transfusion therapi

129 In tissue culture, DLL1 induced proliferation of human peri

130 Transfer to a tissue culture environment resulted in rapid and extensi

131 cells in vitro and in three-dimensional lung tissue cultures ex vivo.

132 togenic components in three-dimensional lung tissue cultures ex vivo.

133 ion of this glycoproteomic platform to human tissues cultured ex vivo.

134 etaOs stems largely from rodent-derived cell/tissue culture experiments or from transgenic models of

135 Mammalian tissue-culture experiments suggest that downstream of MS

136 Pulldown assays from Arabidopsis thaliana tissue culture extracts with the mitochondrial MORF1 and

137 have a small effect on virus replication in tissue culture, few studies have confirmed this mechanis

138 rage-dependent cells (OV-90AD) were grown in tissue culture flasks, whereas anchorage-independent cel

139 NA prepared from 10-fold serial dilutions of tissue culture fluid containing DENVs suggested that the

140 exing and sonication was more sensitive than tissue culture for prosthetic joint infection (PJI) diag

141 is sonication samples is more sensitive than tissue culture for the microbiologic diagnosis of prosth

142 We initiated tissue culture from sliced graft junctions and selected

143 ielded to the emerging tools for in vitro 3D tissue cultures, functional brain-like tissues have not.

144 ric and nonpolymeric fractions from roots of tissue culture-grown plants, (2) the suberin-associated

145 ted transformation through the generation of tissue culture had limited success for Setaria viridis,

146 e VZV genome when the virus is propagated in tissue culture.IMPORTANCE Viruses isolated from clinical

147 er time, mutations accumulate more slowly in tissue culture, in part because of the inefficiency of r

148 e strains replicate with WT-like kinetics in tissue culture, in vivo infections reveal a strong corre

149 ransformation in addition to those caused by tissue culture-induced somaclonal variation.

150 approach could be used for quantification of tissue culture-induced variation and provided direct evi

151 lymorphism analysis, was used to investigate tissue culture-induced variation in triticale regenerant

152 genetic background of donor plants affected tissue culture-induced variation.

153 didate vaccine (low dose [1.5 x 10(4) median tissue culture infection doses (TCID50) per 0.05 mL], me

154 In a tissue culture infection model, pretreatment of C. turic

155 ment and pathogen-mediated cytotoxicity in a tissue culture infection model.

156 chnique's efficacy, we performed time course tissue culture infections using Rift Valley fever virus

157 An optimal dose of 10(7) tissue culture infectious dose 50 was reached that cause

158 se-dependent reduction (0.7 to 1.5 log10 50% tissue culture infectious dose [TCID50]) in nasal wash v

159 nd intranasal inoculation with 7 x 10(6) 50% tissue culture infectious dose of the MERS-CoV isolate H

160 amuscular injection at a dose of 1 x 109 50% tissue culture infectious dose on treatment days 22, 36,

161 tive after 16 weekly vaginal exposures to 50 tissue culture infectious dose SHIV162p3.

162 EBOV-Kikwit, between 4 and 27 times the 50% tissue culture infectious dose, were sufficient to cause

163 developed a human herpesvirus 8 (HHV-8) 50% tissue culture infective dose (TCID50) assay using the T

164 BOV dilution from 4 x 10(7) to 4 x 10(2) 50% tissue culture infective dose (TCID50)/ml, as well as th

165 No surviving virus was revealed by a 50% tissue culture infective dose assay after the combined t

166 t serum (EBOV concentration of 1 x 10(8) 50% tissue culture infective dose per milliliter [TCID50 . m

167 particles) or MVA-BN-Filo (1 x 10(8) median tissue culture infective dose) and boosted with the alte

168 LD50) was determined to be 0.015 50% TCID50 (tissue culture infective dose) of MARV/Ang-MA in SCID mi

169 eductions in viral shedding, based on median tissue culture infective dose, were observed in patients

170 Doses ranging from 10(7) to 10(9) 50% tissue culture infective doses (TCID50) consistently inf

171 cts received vaccine containing 10(7) median tissue culture infective doses (TCID50) of MVA-BN, 10 su

172 a 50% lethal dose (LD50) of 5.3 x 10(-2) 50% tissue culture infective doses (TCID50), was developed.

173 ic range of 8 log units and 1.3 x 10(-3) 50% tissue culture infective doses (TCID50)/ml of cultured M

174 sages ranged from 6 x 10(2) to 2 x 10(5) 50% tissue culture infective doses (TCID50)/ml.

175 We report detection limits of 12 50% tissue culture infective doses (TCID50s) for HIV-1 and 8

176 ferrets intranasally at a dose of 10(6) 50% tissue culture infective doses in a range of inoculum vo

177 s ( approximately 10(6) genome copies or 50% tissue culture infective doses/ml).

178 Our results demonstrate that, at least in tissue culture, influenza virus receptor-binding activit

179 from colon carcinoma grown on semipermeable tissue culture inserts, we determined that infection wit

180 co-cultured with CD14(-) cells separated by tissue culture inserts.

181 one to reconcile conflicting observations in tissue culture, intact isolated ganglia and living anima

182 dification methods require regeneration from tissue culture, involving complicated, long and laboriou

183 sis (Arabidopsis thaliana) using plate-based tissue culture is a very common and relatively rapid ass

184 In tissue culture, it showed about a 6-fold increase in its

185 lternative model for early drug development, tissue culture, lacks both the architecture and, usually

186 e, we describe the generation of Arabidopsis tissue culture lines in which the expression of PRX33 an

187 Using a tissue culture M cell model, we examined S Typhi strains

188 n, a phenomenon in which plants derived from tissue culture manifest phenotypic variability.

189 only are the levels of glutamine in standard tissue culture media at least ten-fold higher than other

190 n hepatoma cell line Huh7.5 by supplementing tissue culture media with human serum (HS) and examined

191 12 mouse myotubes were incubated in standard tissue culture media, or media supplemented with 28 mM g

192 The tissues were preserved for 7 days in tissue culture medium at 31 degrees C.

193 the presence of AGS cells or in purine-free tissue culture medium that has been conditioned by AGS c

194 aid in a sterile basin partially filled with tissue culture medium.

195 ith 1 mL mounted syringe was filled with the tissue culture medium.

196 Costly, genotype-dependent tissue culture methods are used in many crops, while see

197 Plant tissue culture methods need optimization and simplificat

198 influenza virus replication in a human lung tissue culture model and observed strong inhibition of v

199 anges of signature miRNAs were examined in a tissue culture model of HCV in hepatoma cells: HCV infec

200 5 genetic variants in HEK 293 cells and in a tissue culture model of HCV infection revealed that the

201 We have developed a tissue culture model to study the epigenetic basis for l

202 nged Drp1 inhibition reduces DA closure in a tissue culture model.

203 While tissue culture models are extremely useful in understand

204 3D tissue culture models are utilized to study breast cance

205 Whereas studies in 2-dimensional (2D) tissue culture models have suggested that vinculin negat

206 r data showed that despite the fact that all tissue culture models lack a functional adaptive immune

207 Standard tissue culture models lack critical emergent properties

208 ein has multiple immunomodulatory effects in tissue culture models of infection, including NF-kappaB

209 A libraries in loss-of-function screening in tissue culture models provides an effective means to ide

210 eactivation, and we show here that indeed in tissue culture models this cellular transcription factor

211 roviding new routes toward advanced cell and tissue culture models to better understand human biology

212 Using two established tissue culture models, we demonstrate here that adaptive

213 hotgun mass spectrometry analysis founded on tissue culture models, we identified a candidate SIRT2 d

214 By combining in vivo and tissue culture models, we show here that VEGF165-induced

215 functional impact of physical symmetry in 3D tissue culture models.

216 ons and is more similar to that of classical tissue culture models.

217 essing the relevance of observations made in tissue culture models.

218 ructure in APPswe/PS1E9 transgenic mouse and tissue culture models.

219 cation, following bile duct ligation, and in tissue culture models.

220 restriction on PCO using human lens cell and tissue culture models.

221 In fact, reducing levels of SF3B2 in tissue-cultured neurons was effective against neurotoxic

222 those with sonicate fluid and periprosthetic tissue culture obtained at revision or resection arthrop

223 However, tissue cultures of cardiomyocyte monolayers currently re

224 Sensitivities of tissue culture, of sonicate fluid culture, and of PCR we

225 tic treatments used to dissociate cells from tissue culture or fresh tumour specimens cause destructi

226 Transformation procedures required no tissue culture or selectable marker genes.

227 xperiment is carried out in model organisms, tissue culture, or in vitro; typically addresses only a

228 ed vaccines (chicken embryo origin [CEO] and tissue culture origin [TCO]), although effective, can re

229 Three-dimensional organotypic tissue culture (OTC) is an innovative three-dimensional

230 Sonicate fluid PCR was more sensitive than tissue culture (P = 0.04).

231 asis, but has not been studied using dynamic tissue culture paradigms.

232 a deletion that arose during the process of tissue culture passage can be repaired, with subsequent

233 44I mutation in the Makona GP1,2 gene during tissue culture passage in certain cell lines.

234 primary isolate that arose after 21 weeks of tissue culture passage in the presence of inhibitor.

235 set of secondary adaptation mutations after tissue culture passage.

236 ssue cultures and rodents, with intermittent tissue culture plaque purifications, to produce a virus

237 varying substrate compliance (4-71 kPa) and tissue culture plastic (TCP) (> 1 gigapascal [GPa]).

238 oncede that standard cell culture materials (tissue culture plastic and glass) do a poor job of recap

239 oth, micro, and nano) and a control surface (tissue culture plastic) with or without osteogenic suppl

240 PDL cells were plated for various times on tissue culture plastic, PDL-derived ECMs, collagen Type

241 ng-term propagation of hPS cells on uncoated tissue culture plastic.

242 brin gels prepared in advance in a multiwell tissue culture plate.

243 ptides were synthesized and used to pre-coat tissue culture plates before lung derived ASM cells and

244 uential delivery of the mixed solutions into tissue culture plates is actuated by a novel mechanism b

245 as NIH3T3 cells that express it form foci in tissue culture plates, colonies in soft agar, and tumors

246 ifferent induction periods and using various tissue culture plates.

247 ion and cell proliferation between brands of tissue culture plates.

248 We describe herein a three-dimensional (3D) tissue culture platform using a polydimethylsiloxane (PD

249 Biomaterial-based tissue culture platforms have emerged as useful tools to

250 C-NSCs) cultured on PEG hydrogels or treated tissue culture polystyrene (TCP) surfaces.

251 kPa) and glaucomatous meshworks (75 kPa), or tissue culture polystyrene (TCP; >1 GPa).

252 ) depended on previous culture time on stiff tissue culture polystyrene (TCPS; E ~ 3 GPa).

253 ated when VICs are cultured on stiff gels or tissue culture polystyrene compared with freshly isolate

254 em cells to densities comparable to those of tissue culture polystyrene controls (TCPS).

255 col) hydrogels, poly(dimethyl siloxane), and tissue culture polystyrene.

256 4T1 murine breast cancer cells derived from tissue culture, primary mammary tumors, and pulmonary me

257 iewpoint were used to compare periprosthetic tissues culture processes using conventional techniques

258 We compared the standard tissue culture protocol, using media supplemented with 1

259 could be purified and expanded in number in tissue culture, Richard Bunge in 1975 envisioned that th

260 ethod can be easily implemented in a typical tissue culture room by personnel with standard mammalian

261 NAs products in virus-free transgenic papaya tissue culture seedlings.

262 analysis in combination with ex vivo insect tissue culturing shows that two generalist isolates of t

263 We made use of stable isotope labeling in tissue culture (SILAC) to identify ISGylated proteins th

264 healthy tissue from tumor tissue, and basic tissue culture skills.

265 After serial passage in tissue culture, some viruses partially recovered fitness

266 methylation is stochastically induced at the tissue culture step.

267 duction time (which is in most cases free of tissue culture steps), and the implementation of confine

268 Both ex vivo stimulation and in vitro tissue culture studies demonstrated that the activation

269 Tissue culture studies demonstrated that the S224A, S224

270 Additional tissue culture studies showed a PDGFR-dependent regulati

271 l system for three-dimensional (3D) cell and tissue culture, studies of fibrillogenesis, and investig

272 lls were seeded on the AuNRs compared to the tissue-culture surface.

273 Using a tissue culture system that supports the development of h

274 Here we use a novel tissue culture system to study a signaling pathway that

275 e infected cells in a two-chamber trans-well tissue culture system.

276 on of models for SAC regulation developed in tissue culture systems and demonstrate that several fund

277 burden to society, yet the lack of reliable tissue culture systems has hampered the development of a

278 dies conducted in humans, animal models, and tissue culture systems have enhanced our understanding o

279 cell line can be used in long-term (8 wk) 3D tissue culture systems.

280 chanistic inferences remains to be tested in tissue culture systems.

281 ood culture bottle system for periprosthetic tissue culture [T.

282 ssaged stocks of GPCMV are pathogenic, while tissue culture (TC) passage in fibroblasts results in at

283 For conventional periprosthetic tissue culture techniques, the greatest accuracy was obs

284 we used a BTV8 strain minimally passaged in tissue culture (termed BTV8L in this study) and a deriva

285 hmallenberg virus (SBV) serially passaged in tissue culture (termed SBVp32) unexpectedly displayed in

286 rmality is a somaclonal variant arising from tissue culture that drastically reduces yield, and has l

287 larized light backscattered from GNRs within tissue culture, the ensemble-averaged translational self

288 In tissue culture, TNC was superior amongst several extrace

289 We used three-dimensional tissue culture to build an organotypic model of bronchia

290 After passaging these viruses in tissue culture to select for functional variants, we use

291 ined medium and in traditional conditions on tissue culture treated (TCT) plastic in fetal bovine ser

292 level of adherence as those cells seeded on tissue culture-treated surfaces, by 4 d cell numbers and

293 ype compared with those cultured on standard tissue culture-treated surfaces.

294 ge of transgenic lines through two different tissue culture treatments resulted in Tnt1 transposition

295 in hamster cheek pouch topically exposed to tissue culture trypomastigotes (TCTs).

296 In tissue culture, VZV-infected human neurons remain viable

297 Results of tissue cultures were negative.

298 This effect was duplicated in tissue culture, where coculture of cancer cells with tum

299 Human corneal tissue culture with 100 microg/ml concentrations of SiNP

300 bine the facile manipulation and readouts of tissue culture with the virus-relevant complexity of ani