ライフサイエンスコーパス: to demonstrate

1 selectively binds different non-haem metals to demonstrate 30-fold and 11-fold enhancements in the o

2 osphere radiocarbon disequilibrium estimates to demonstrate a approximately 689+/-53 (14)C-yr increas

3 cal studies and pharmacological interference to demonstrate a central role of Ptpn11 in postnatal myo

4 Taken together, these results are the first to demonstrate a clear role for SP1 in the up regulation

5 This is the first study to demonstrate a cognitive role related to alERC volume

6 istry, and the Daun02 inactivation procedure to demonstrate a critical role of dorsomedial striatum n

7 This study seeks to demonstrate a dynamic correlation between two such af

8 To demonstrate a functional ES-TUBB3 axis in cutaneous h

9 with preprocedural CT angiography that helps to demonstrate a lack of attenuation in the cervical ICA

10 These results are the first to demonstrate a link between changes in the conserved i

11 Cbl/Cbl-b DKO) using Lgr5-EGFP-IRES-CreERT2, to demonstrate a mammary epithelial cell-autonomous requ

12 ate, no randomized studies have been powered to demonstrate a mortality or stroke reduction benefit o

13 s of YARS2-related mitochondrial disease and to demonstrate a new Scottish founder variant.

14 The solver is utilized to demonstrate a novel type of leaky-guided acoustic wav

15 To demonstrate a potential application of the paper-base

16 device with real-time analysis capabilities to demonstrate a potential point-of-care application.

17 The first aim was to demonstrate a previously hypothesized increased sensi

18 FE is unknown, and prior studies have failed to demonstrate a primary abnormality in the eosinophil l

19 lectric nanorobot is designed and fabricated to demonstrate a proof-of-concept integrated device, whi

20 These sensitive ion detectors are used to demonstrate a proof-of-concept low-cost, amplifier-fr

21 lesteryl ester transfer protein) have failed to demonstrate a reduction in risk for coronary heart di

22 e, we used recipient mice deficient in STAT6 to demonstrate a requirement for this critical component

23 strongly coupled to a nanophotonic resonator to demonstrate a significant cavity protection effect at

24 Here, we deploy CRISPR/Cas9 technology to demonstrate a variety of sophisticated genetic modifi

25 hat is, human immunoglobulin G, with the aim to demonstrate a virtually universal protein detection p

26 pt by using a nanoelectromechanical platform to demonstrate active tuning.

27 This case is the first to demonstrate allosensitization following minor surgery

28 MicroRNAs have previously been reported to demonstrate altered expression in several aging pheno

29 This is the first investigation to demonstrate an acute level trade-off between these tw

30 The findings are the first to demonstrate an association between ketamine's clinica

31 , patients in the PEX group were more likely to demonstrate an ECG abnormality than in the non-PEX gr

32 sional array of these devices was sufficient to demonstrate an efficient Hamming distance comparator

33 sions of the LC and the noradrenergic system to demonstrate an exacerbation of cognitive deficits, ne

34 theory, experiments, and numerical analysis to demonstrate an inherent trade-off between the informa

35 ommonly used today.The aim of the article is to demonstrate and compare the effects of two IR algorit

36 The aim of this work was to demonstrate and to evaluate the analytical performanc

37 This is the first study to demonstrate associations between the gut microbiota a

38 which apelin signaling is downregulated, and to demonstrate attenuation of PAH severity with exogenou

39 evels, but further studies will be necessary to demonstrate benefit with this technology.

40 ting of newly identified variants is crucial to demonstrate causality.

41 2017) use elegant MADM-based lineage tracing to demonstrate cell-intrinsic and global functions for L

42 ponds to plate bending and an internal scale to demonstrate changes in the pin position on plain film

43 t least 1 of the confirmatory studies failed to demonstrate clinical benefit in 2 (8%), were terminat

44 Metaanalyses failed to demonstrate clinical benefits of beta lactam plus ami

45 e NW and SE Elysium lava fields is the first to demonstrate compositional variation within a single v

46 y ligation assays in endothelial cells (ECs) to demonstrate compound-induced inhibition of Pfn1-actin

47 a self-accelerating optical pump wave-packet to demonstrate controlled shaping of one type of general

48 Additionally, solid-state NMR was used to demonstrate correlation between the crystal structure

49 To demonstrate critical techniques and clinical outcome

50 opy Decomposition and ZetaOtu classification to demonstrate cryptic diversity between closely related

51 We apply this strategy to demonstrate differential mRNA trafficking behaviors g

52 overexpression with recombination substrates to demonstrate direct consequences of BCL11A/RAG modulat

53 We applied atomic force microscope (AFM) to demonstrate directly that on-surface aggregation take

54 determinant quantum Monte Carlo calculations to demonstrate dynamical stripe correlations in the thre

55 In order to demonstrate EDL behaviour, a sputtered 200 nm-thick S

56 in observed a short half-life, but were able to demonstrate effective FcgammaR blockade.

57 anemia (FA) gene therapy studies have failed to demonstrate engraftment of gene-corrected hematopoiet

58 e authors develop a witness which allow them to demonstrate entanglement between millions of atoms in

59 However, attempts to demonstrate enzyme-free, template-directed synthesis

60 tes and humans; thus it has been challenging to demonstrate experimental proof of causality of these

61 Early anti-VEGF administration seems to demonstrate favorable results.

62 To demonstrate feasibility and protective efficacy, nucl

63 , and humanized NOD-scid IL-2Rgammanull mice to demonstrate for the first time that: i) mouse and hum

64 is is the first bivariate GWAS meta-analysis to demonstrate genetic factors with pleiotropic effects

65 With this in mind, we sought to demonstrate how deep mutational scanning (DMS) could

66 n 587 Agta collected during recent fieldwork to demonstrate how multiple partial age ranks coming fro

67 to restore expression in two null cell lines to demonstrate how our system confirms causality through

68 Examples are also shown to demonstrate how phenotype paths in phenotype ontology

69 hetic polypeptide-grafted comb macromolecule to demonstrate how the in situ modulation of interaction

70 T/CT scanner is currently under construction to demonstrate how this step change in sensitivity affec

71 The primary objective was to demonstrate improvement in progression-free survival

72 We used the engineered Fc domains to demonstrate in vitro and in mouse models that for the

73 ase 2A (PDE2A) inhibitors have been reported to demonstrate in vivo activity in preclinical models of

74 To demonstrate independent reading and writing, we built

75 ed through protein A affinity chromatography to demonstrate industrial applicability at large scale.

76 lifetime microscopy (FLIM)-based biosensing to demonstrate intratumoral heterogeneity of EGFR activi

77 (CD3xHER2, CD3xEGFR, EGFRxHER2) synthesized to demonstrate its broad applicability.

78 To demonstrate its efficiency, DMRfinder is benchmarked

79 Moreover, to demonstrate its generality, the method is used to cor

80 r age-related macular degeneration (AMD) and to demonstrate its use to model the impact of specific c

81 To demonstrate its utility, we applied the framework to

82 To demonstrate its utility, we used ME-Class to analyze

83 PMPC is the only congener within this series to demonstrate micromolar antihuman cytomegalovirus (HCM

84 n Ca(2+)/calcineurin and Cdc42 signaling and to demonstrate modulation of Cdc42 activity through a GA

85 chottky analysis on the same sensor platform to demonstrate multi-configurable modes.

86 Here, whole-genome sequencing was used to demonstrate nosocomial acquisition of antimicrobial-r

87 imulated and recorded epileptic seizure data to demonstrate our system's effectiveness.

88 gy metabolism can be repeatedly investigated to demonstrate penumbra tissue, which can benefit from t

89 We use transplant experiments to demonstrate plasticity in the fate choice between col

90 ecipitation with menin or ubiquitin was used to demonstrate post-translational modification of menin.

91 s of melanoma and non-melanoma skin cancers, to demonstrate potential clinical utility.

92 To demonstrate practical utility, we analyzed the proteo

93 To demonstrate PRINCESS' performance and feasibility, we

94 tized triangular sizes, but they have failed to demonstrate production and control of isolated defect

95 To demonstrate proof-of-principle, we used the TRBO-sgRN

96 We aim to demonstrate real-time optical sectioning using a near

97 downstream purification process is designed to demonstrate robust removal of these impurities.

98 Different terms were found to demonstrate seasonality depending on the country - na

99 ning Electron Microscopy (SEM) has been used to demonstrate selective immobilization of each AuNP on

100 These results are the first to demonstrate sex differences in BLA neuronal activity

101 5 decades, making it increasingly difficult to demonstrate significant benefits of new therapies in

102 owever, prospective clinical trials have yet to demonstrate significant efficacy.

103 ack-athletic stereotypical association fails to demonstrate similar modulations by cardiac cycle.

104 t, Nd/Sm and Nd/Dy are used as binary models to demonstrate solid/aqueous and solid/solid separation

105 ht scattering, and impedimetric spectroscopy to demonstrate stability in detection of analytes, Inter

106 Here, we have used a genetic approach to demonstrate that activation of the SHH pathway is nec

107 ns following myelosuppressive injury in mice to demonstrate that aged ECs impair the repopulating act

108 eening and genetic analysis in our new model to demonstrate that alpha-synuclein expression promotes

109 ed ex vivo spinal cord slice cultures (SCSC) to demonstrate that anti-inflammatory mechanisms may als

110 and tomography of staged C. elegans embryos to demonstrate that BBs remodel to support ciliogenesis

111 and machine learning approaches were applied to demonstrate that by integrating the electrical and ki

112 e further generated Casp2(C320S) mutant mice to demonstrate that caspase-2 catalytic activity is esse

113 cally defined to synthesize only desmosterol to demonstrate that cholesterol is important at stages f

114 we use a structure-based mutagenic approach to demonstrate that cobalamin riboswitches have a broad

115 The purpose of this work is to demonstrate that decreasing the strength of this HBI,

116 d animal models and cultured mammalian cells to demonstrate that disruption of the methylation pathwa

117 Finally, we use a genetic approach to demonstrate that dLDH and L-2HG influence position ef

118 amily design of the sample, we are also able to demonstrate that epigenetic aging and white matter tr

119 nd transposon-mediated somatic gene transfer to demonstrate that expression of either the endogenous

120 se several genetically modified mouse models to demonstrate that expression of the multifunctional BR

121 We used a proteomic approach to demonstrate that fibroblasts selectively increase deg

122 Our work is the first to demonstrate that genetic mixture can alter the course

123 n disease traits and experimental validation to demonstrate that genetic variation affects expression

124 homogeneous but smaller cohort, we were able to demonstrate that GG-CTLA4-CBU was associated with inc

125 rmore, we used chromatin immunoprecipitation to demonstrate that H3 histones at the Rgs10 proximal pr

126 In conclusion, we are the first to demonstrate that HIF-1alpha is a key regulator of glu

127 re we use functional and structural analyses to demonstrate that HLA-C expression is modulated not ju

128 g both in vitro and in vivo models, we go on to demonstrate that hydralazine has neuroprotective prop

129 We use agent-based numerical simulations to demonstrate that if average lengths of actin filament

130 ic, tamoxifen-activated, PKP2 knockout mouse to demonstrate that in addition to its role in cell adhe

131 t autochthonous mouse model of breast cancer to demonstrate that in vivo TMP195 treatment alters the

132 diated GABAergic-specific GCaMP6f expression to demonstrate that inhibitory neurons in ferret visual

133 pacemaker and its response to light was used to demonstrate that Irregular vs. Regular group differen

134 analytical ultracentrifugation was utilized to demonstrate that isolated TM domains of Hendra virus

135 Experiments are then used to demonstrate that it is indeed possible to create stab

136 and used a cell-surface biotinylation assay to demonstrate that it traffics to the plasma membrane,

137 take advantage of the high resolution of TEM to demonstrate that laser-pulled quartz nanopipets repro

138 the intervening chromatin loops, and use it to demonstrate that malignant transformation compromises

139 lectrophysiological, and behavioral measures to demonstrate that metabotropic glutamate receptor-indu

140 ertion measurements complement these studies to demonstrate that mVP40 does not appreciably insert in

141 article dismisses all prior work purporting to demonstrate that number is a conceptual primitive.

142 This system was used to demonstrate that oscillations are a collective proper

143 arthritis model was established enabling us to demonstrate that pain was blocked by a cyclooxygenase

144 the first, to our knowledge, direct evidence to demonstrate that PCL proteins are crucial for PRC2 re

145 studies employ stringent clinical standards to demonstrate that perceptual learning on a computerize

146 ic kidney cell line HEK293T, it was possible to demonstrate that PGC1alpha alone can support viral bi

147 More significantly, we were able to demonstrate that polar secretion of substrates is cri

148 oped modular reporter system proved possible to demonstrate that PpMmsR/P mmsA and PpHpdR/P hpdH are

149 or research conducted in the learning domain to demonstrate that predictability moderates the effects

150 Till date, studies have failed to demonstrate that preventing gastroesophageal reflux w

151 ined empirical data with computer simulation to demonstrate that RAG-2 mice intravenously infected wi

152 transfer, pulldown, and in vitro GEF assays to demonstrate that regulation of GEF activity is achiev

153 rs use an amputation assay in Xenopus laevis to demonstrate that removal of the brain early in develo

154 We have done this to demonstrate that reptarenaviruses cause inclusion bod

155 We used single-molecule imaging to demonstrate that Saccharomyces cerevisiae condensin i

156 We use a numerical landscape evolution model to demonstrate that short-wavelength deformation causes

157 netics with calcium imaging and pharmacology to demonstrate that stimulation of NPVF cells suppresses

158 of rats with chronic temporal lobe epilepsy to demonstrate that subsets of cells discharge in a high

159 These findings are the first to demonstrate that T can rapidly (within 60 minutes) po

160 assays and heterologous expression in planta to demonstrate that TcADH2 encodes an enzyme that oxidiz

161 tion and chemical and biochemical approaches to demonstrate that tellurite inhibits heme biosynthesis

162 Here, we use a series of biochemical assays to demonstrate that the 3'-terminal portion of the nucle

163 th deuterated substrates are also shown here to demonstrate that the 6'-pro-R-hydrogen atom of GenX2

164 avenous methamphetamine self-administration, to demonstrate that the activation of monosynaptic gluta

165 Here, we use thermostability assays to demonstrate that the carrier is regulated by calcium

166 We were able to demonstrate that the compounds have a clear effect on

167 we use both synthetic and experimental data to demonstrate that the determination of epsilon-values

168 Here we use a 'multi-omics' approach to demonstrate that the duration of polymerase pausing g

169 and population receptive field (pRF) mapping to demonstrate that the fine-grained functional architec

170 opulation structure, and coalescent modeling to demonstrate that the golden-crowned manakin is geneti

171 This well-conducted trial failed to demonstrate that the intervention kept children carie

172 First, we use molecular simulations to demonstrate that the IRMOF-74 series undergoes a defo

173 Together, these results are the first to demonstrate that the left angular gyrus is critical f

174 and deuterated lipid products, we were able to demonstrate that the magnitude of the PKIE measured i

175 To demonstrate that the mass resolution was truly increa

176 ics, proteomics, and whole-genome sequencing to demonstrate that the MMAR_0039 gene is not required f

177 The goal of this study was to demonstrate that the NSP4 VPD forms an ion channel an

178 with mu-Raman spectroscopy have been applied to demonstrate that the obtained microparticles consist

179 We used optogenetics to demonstrate that the pedunculopontine tegmental nucle

180 ssays with fluorescently labeled polymerases to demonstrate that the Pol III* complex (holoenzyme lac

181 We capitalized on these interactions to demonstrate that the Pro33 coding variation in the mu

182 e then utilize atomic force microscopy (AFM) to demonstrate that the redox state of the [4Fe4S] clust

183 We used Random Forests modelling to demonstrate that the site and salinity of a sample co

184 ady-state oxic and anoxic chemostat cultures to demonstrate that the switch from aerobic to anaerobic

185 direct cleavage of their target transcripts to demonstrate that the tRF-AGO1 complex acts to specifi

186 f biomechanics, fluid dynamics, and robotics to demonstrate that there is a fundamental difference be

187 centrations and high numbers of ion channels to demonstrate that there is an upper limit of quantific

188 efield calcium imaging in mouse visual areas to demonstrate that these seizures start as local synchr

189 er et al. (2017) use in vivo lineage tracing to demonstrate that these two possibilities may occur do

190 tein genes, and piggyBac transposase vectors to demonstrate that this can be a reliable, efficient, a

191 This is the first field experiment to demonstrate that this modified ISCO approach coupled

192 s, cell biological and pharmacological tools to demonstrate that TMEM16A, an evolutionarily conserved

193 we use comparative and transgenic approaches to demonstrate that two differentially transcribed, co-d

194 To demonstrate that use of RPD does not increase the inc

195 ng, immunofluorescence, and confocal imaging to demonstrate that virtually all NTS-->lPBN and lPBN-->

196 t a compilation of precipitation proxy data, to demonstrate that while asymmetric extratropical forci

197 subsequent click chemistry-based detection, to demonstrate that zDHHC enzymes have marked difference

198 This blinded image evaluation (BIE) sought to demonstrate that, after limited training, readers nai

199 arcoding of macaque hematopoietic stem cells to demonstrate that, after transplantation, HSCs are ver

200 Labeling the entire cell membrane allows one to demonstrate that, by contrast with fluorescence, ECL

201 dividual, using a mechanistic learning model to demonstrate that, perhaps surprisingly, this populati

202 Small-angle X-ray scattering was used to demonstrate that, upon complex formation with target

203 the human alpha1beta2gamma2L GABAA receptor to demonstrate that: 1) curvilinear isoboles of additivi

204 We performed analyses to demonstrate that: 1) These filters impacted variants

205 xperimental, acute, and persistent phenomena to demonstrate the advantages of adopting a statistical

206 However, clinical data have yet to demonstrate the analgesic efficacy of inhibitors of e

207 a backend with a novel streaming.mzML parser to demonstrate the API-based serving of MS data to the v

208 ions with the 2D and 3D memristive crossbars to demonstrate the applicability of such 3D CMOL hybrid

209 To demonstrate the applicability of the proposed strateg

210 To demonstrate the capabilities of our method, we design

211 has allowed us to validate the new cell and to demonstrate the capabilities of this technique to obt

212 well as anthropogenic systems, as an example to demonstrate the capability of Formularity with IPA.

213 fferent polarities is used as a model sample to demonstrate the capability of the developed ionizatio

214 The primary objective was to demonstrate the clinical applicability of ZTE in osse

215 patients with indications for a thyroid scan to demonstrate the clinical safety and diagnostic effica

216 To our knowledge, this is the first study to demonstrate the clinical utility of predictive geneti

217 To demonstrate the clinical value of our platform, human

218 To demonstrate the concept, a 0.5 numerical aperture (NA

219 been hampered by the lack of biomarkers able to demonstrate the course of the disease.

220 st of our knowledge, this is the first study to demonstrate the crucial role of reactive oxygen speci

221 This is the first report to our knowledge to demonstrate the dose-related efficacy of R/S-ketamine

222 the form of nanoporous gold as a case study to demonstrate the dynamic behaviour of bimetallic syste

223 To demonstrate the effectiveness of MS-FLO, we processed

224 riments in humans and animals were conducted to demonstrate the effects of modifying the TFS in natur

225 To demonstrate the efficacy and safety of once-daily nep

226 (A Clinical Trial to Demonstrate the Efficacy of Cangrelor [PCI]: NCT00305

227 gram-negative waterborne pathogens were used to demonstrate the efficacy of the microchip based DNA e

228 Purpose To demonstrate the feasibility of contrast material-enha

229 Purpose To demonstrate the feasibility of imaging-guided cathete

230 man transferrin, and bovine fetuin as models to demonstrate the feasibility of the method.

231 rotein (AFP) and cancer antigen 125 (CA125)] to demonstrate the feasibility of the proposed method.

232 denreich (TF) antigen conjugate was obtained to demonstrate the feasibility of this methodology, whic

233 The objective of this study was to demonstrate the feasibility of using micro-CT scan of

234 sly we developed an in vitro assembly system to demonstrate the formation of filament structures with

235 phores in different domains were constructed to demonstrate the function of light harvesting in a pol

236 Our study is the first to demonstrate the functional impact of a drug of abuse

237 Our study is the first to demonstrate the involvement of DNA methylation-associ

238 clear picture of the defect physics involved to demonstrate the local modulation of the electronic pr

239 rable states and basic photon interferometry to demonstrate the locally-correlated quantum advantage.

240 blinded independent investigators are needed to demonstrate the long-term safety, efficacy, and durab

241 To demonstrate the potential applicability of the techni

242 Finally, this work serves to demonstrate the potential for naphthazarin as a build

243 In order to demonstrate the potential of CCS prediction, the stra

244 To demonstrate the potential of FIMTrack we provide an e

245 A red wine sample was analyzed to demonstrate the potential of the longitudinal on-colu

246 Moreover, to demonstrate the potential of this approach for integr

247 ity, three independent studies are performed to demonstrate the potential of this platform for: i) tr

248 ions that have little experimental precedent to demonstrate the potential utility of the model to pre

249 To demonstrate the power of AmpSeq-SSR, we adopted it in

250 To demonstrate the power of image-based pooled screening

251 This has allowed us to demonstrate the presence of longitudinal and transver

252 , we report a DNA-based genotyping test used to demonstrate the presence of the resistant (SfABCC2mut

253 The aim of this study was to demonstrate the proviral role of PLK1 in HBV biosynth

254 t blood glucose concentrations were utilized to demonstrate the quick response of the patterned FRET

255 of the mechanical competence of the hip and to demonstrate the reproducibility of the tool.

256 ability in tropical forests, and use a model to demonstrate the resulting impacts on regional air qua

257 To demonstrate the robustness of [(3)H]RO6957022 binding

258 detecting disease and guiding treatment and to demonstrate the role of cardiovascular imaging in fac

259 Cryo-EM reconstruction was used to demonstrate the structure of T = 1 assemblies templat

260 The aim of this study is to demonstrate the superior optical and mechanical prope

261 on, and measurement data have been presented to demonstrate the superior performance of the 3D micro

262 filed 68k peripheral blood mononuclear cells to demonstrate the system's ability to characterize larg

263 -scale perfusion cultures (350 mL) were used to demonstrate the technology, which can be scaled-out w

264 d nickel were generated on copper substrates to demonstrate the underlying CL dynamics.

265 The purpose of this article is to demonstrate the universality of this quantum phase tr

266 To demonstrate the usability of the tool, three examples

267 To demonstrate the usefulness of this device and the pow

268 To demonstrate the utility of CLAM, we applied it to a w

269 To demonstrate the utility of our software, we subjected

270 To demonstrate the utility of the algorithm, we also app

271 To demonstrate the utility of this system, we show that

272 To demonstrate the value of the model, we extracted a va

273 eptide (GIP), as proof-of-principle peptides to demonstrate the value of thioamide substitution.

274 To demonstrate the versatility of the approach we have f

275 In addition to demonstrating the potential for consensus design of g

276 In vitro studies were instrumental to demonstrating the role of ang II in inducing podocyte

277 d single-chain nanoparticles (Pt(II) -SCNPs) to demonstrate their application as a recyclable homogen

278 ing, despite a lack of high-quality evidence to demonstrate their efficacy.

279 To demonstrate their practical applicability, the sensor

280 g assays with unlabeled competitors in order to demonstrate their suitability for drug discovery purp

281 utions as well as simulated seawater samples to demonstrate their usefulness as sensors in a deep-sea

282 Finally, to demonstrate this applicability, glucose could be dete

283 To demonstrate this concept, planar and 3D-structured sh

284 To demonstrate this technique's generality, we exemplify

285 We generate double-gene deletion libraries to demonstrate this technology, targeting antifungal eff

286 To demonstrate this, we de novo-created promoters for bi

287 e use the reconfigurability of these devices to demonstrate three prominent QKD protocols-BB84, Coher

288 erial compositions is presented, enabling us to demonstrate TPA-free CMOS-compatible waveguides with

289 al growth factor (VEGF) pathways have failed to demonstrate tumor control.

290 l intense stimulus (high-intensity exercise) to demonstrate two classic features of a habituated resp

291 se data on over 50,000 Drosophilid fly wings to demonstrate unexpectedly strong positive relationship

292 Thus, this is the first to demonstrate use of Salmonella aptamers for developmen

293 To demonstrate utility beyond quantum light sources we s

294 ussion of clinical study designs, approaches to demonstrate vaccine effectiveness, and regulatory pat

295 2 malignant lesions, color Doppler US failed to demonstrate vascularization in four (33.3%) lesions,

296 capacitive sensing method has been reported to demonstrate very promising performance on rapid and s

297 To demonstrate viability, the PAD was used to detect bet

298 ease outcomes, warranting additional studies to demonstrate whether [(18)F]-FDG-PET/CT can identify o

299 Therefore, the goal of this study was to demonstrate whether L1 peptides conjugated to FHs pro

300 of molecular semiconductors and is also used to demonstrate why common textbook approaches fail in de