ライフサイエンスコーパス: toeprint

1 In vitro translation studies and toeprint analyses also indicated that fst expression is

2 Gel mobility shift and toeprint analyses demonstrate that CsrA binds cooperativ

3 Toeprint analyses performed to map the positions of stal

4 Toeprint analysis demonstrated that purified RNA II was

5 Toeprint analysis revealed that in vitro formation of a

6 Additionally, toeprinting analysis of miRNA-targeted mRNAs demonstrate

7 tro translation by E. coli cell extracts and toeprinting analysis of transcripts encoded by the chlor

8 Toeprinting analysis shows that different positions with

9 Toeprinting analysis shows that pre-translocation comple

10 Primer extension inhibition (toeprint) analysis to measure ribosome binding demonstra

11 Primer-extension inhibition (toeprint) analysis with 30 S ribosoma subunits, tRNAMet,

12 Results from toeprint and cell-free translation experiments indicate

13 Toeprint and cell-free translation results indicated tha

14 Toeprint and cell-free translation studies demonstrate t

15 om S. lividans were isolated and included in toeprint and filter binding assays with leadered and lea

16 Additional toeprint and in vitro translation analyses demonstrated

17 Furthermore, the results from toeprint and in vitro translation experiments indicate t

18 Results from gel shift, footprint, toeprint and in vitro translation experiments indicate t

19 Results from toeprint and RNA-directed cell-free translation experime

20 Here we use toeprinting and polysome profiling assays to delineate r

21 Using primer extension inhibition (toeprint) and filter binding assays to measure ribosome

22 By using extension inhibition analysis (toeprinting) and RNA electrophoretic mobility shift assa

23 Results from cell-free translation, ribosome toeprint, and RNA structure mapping experiments demonstr

24 40S subunit binding generated the same toeprint as 80S ribosomes but also additional ones near

25 e of interactions that are not stable in the toeprint assay.

26 79 of the 23 S rRNA, respectively, using the toeprint assay.

27 pseudoknot upstream from the AUG codon, the toeprinting assay revealed 40S ribosomal subunits trappe

28 Here, we used a toeprinting assay to how that streptomycin, neomycin, ka

29 The refined toeprinting assay was used to confirm context-dependent

30 When the toeprinting assay was used with mRNAs that initiate tran

31 chanism using a primer-extension inhibition (toeprint) assay in a homologous N. crassa cell-free tran

32 A primer extension inhibition (toeprint) assay was developed using ribosomes and riboso

33 Here we use a primer extension inhibition (toeprinting) assay to delineate ribosome positioning and

34 bed for using a primer extension inhibition (toeprinting) assay to study the initiation step of prote

35 A primer extension (toeprinting) assay was used to monitor selection by ribo

36 Toeprinting assays showed that CsrA competes effectively

37 Toeprinting assays suggested that CsrA binding causes ch

38 somes, based on primer extension inhibition (toeprint) assays and reporter synthesis assays, a window

39 gal extracts by primer extension inhibition (toeprint) assays showed that these AAPs acted similarly

40 ested as a 2 nucleotide forward shift of the toeprint attributed to pretermination complexes that lea

41 the polypyrimidine residues and generated a toeprint consistent with binding to this region.

42 e-specific regulation was retained revealed "toeprints" corresponding to ribosomes positioned at the

43 ad3.7 and ad3.10, but not wt MetNS3, formed toeprints downstream of the initiator AUG codon in an as

44 Results from ribosome and TRAP toeprint experiments indicated that the ribosome and TRA

45 identical band was also detected in in vitro toeprinting experiments after the addition of YoeB to th

46 identical band was also detected in in vitro toeprinting experiments when YafO was added to the react

47 s unusual regulatory element, we adapted the toeprinting (or reverse transcriptase extension inhibiti

48 repeats were protected by bound TRAP, while toeprint results suggest that nine triplet repeats contr

49 However, extension inhibition experiments (toeprinting) showed that IF3 retained its ability to dis

50 0 nucleotides upstream of this site; and the toeprint signal corresponding to ribosomes at the downst

51 At high arginine concentrations, the toeprint signal corresponding to ribosomes at the uORF t

52 Generally, the IFs reduced the toeprint signal on leadered mRNA; however, incubation of

53 iator tRNA reactions that yielded weak or no toeprint signals still formed complexes in filter bindin

54 while coupled-transcription-translation and toeprint studies demonstrated that CsrA regulates CstA s

55 nation codon rapidly increased; a new, broad toeprint that represents additional ribosomes stalled on

56 analyzed by primer extension) analysis, and toeprinting, we found that (i) 40S subunits bind to BTE

57 independently; mRNA movement was assayed by toeprinting, while tRNA and ASL movement was monitored b