ライフサイエンスコーパス: tombusvirus

1 rified RdRp preparation of Cucumber necrosis tombusvirus.

2 IRV, but not against the related peroxisomal tombusviruses.

3 cation enhancer on the minus-stranded RNA of tombusviruses.

4 s mutant also exhibits strong suppression of tombusvirus accumulation in yeast and in virus-infected

5 between fish nodaviruses and members of the tombusvirus and calicivirus groups provide significant n

6 ecrosis virus (CNV) is a member of the genus Tombusvirus and has a monopartite positive-sense RNA gen

7 ecrosis Virus (CNV) is a member of the genus Tombusvirus and has a monopartite positive-sense RNA gen

8 Tombusviruses and a yeast model host are used to identif

9 tion occurs frequently during replication of tombusviruses and carmoviruses, which are related small

10 In this review, we discuss that tombusviruses and nodaviruses are capable of exploiting

11 cuses on our current knowledge obtained with tombusviruses and other plant viruses.

12 This motif is highly conserved among tombusviruses and related carmoviruses, and it is simila

13 Altogether, Rsp5 is a CIRF against a tombusvirus, and it possibly has a regulatory function d

14 ls, similar in ultrastructural appearance to tombusvirus- and togavirus-induced membrane structures.

15 imilar to other positive-strand RNA viruses, tombusviruses are replicated by the membrane-bound viral

16 s in the family should be sought even though tombusviruses are reputed to be soil transmitted without

17 ed broad-spectrum protection against related tombusviruses but remained susceptible to a distantly re

18 cellular Rpn11 is a critical host factor for tombusviruses by regulating viral replication and geneti

19 In summary, evidence is presented that a tombusvirus can use the recombination signal of BMV.

20 ed replication of Carnation Italian ringspot tombusvirus (CIRV) in yeast.

21 In case of Carnation Italian ringspot tombusvirus (CIRV), the mitochondrial membranes supporte

22 ed replication of Carnation Italian ringspot tombusvirus (CIRV).

23 ly, this work has revealed new functions for tombusvirus cis-acting RNA elements and provided insight

24 st proteins present in the cucumber necrosis tombusvirus (CNV) replicase, we affinity purified functi

25 dent replicase complex for Cucumber necrosis tombusvirus (CNV), which is a plus-stranded RNA virus, f

26 monstrate that the minus-strand templates of tombusviruses contain a replication enhancer, which can

27 f cell-intrinsic restriction factors against tombusviruses contains tetratricopeptide repeat (TPR) do

28 eplicase of Cucumber necrosis virus (CNV), a tombusvirus, contains the viral p33 and p92 replication

29 This regulatory mechanism might explain how tombusviruses could adjust the efficiency of RNA replica

30 hese regulatory mechanisms might explain how tombusviruses could adjust the efficiency of RNA replica

31 Surprisingly, tombusviruses could also exploit expanded ER membranes,

32 We suggest that tombusviruses could sense the status of the infected cel

33 Tomato bushy stunt virus (TBSV) and other tombusviruses encode a p19 protein (P19), which is a sup

34 Tombusviruses express a 19 kDa protein (p19), which has

35 ements, RII(+)-SL (located internally in the tombusvirus genome) and RIV (located at the 3'-terminus)

36 dium bornovanus As with other members of the Tombusvirus genus, the CNV capsid swells when exposed to

37 As expected, being a member of the Tombusvirus genus, the core structure of CNV is highly s

38 yeast as a model host with bromoviruses and tombusviruses have facilitated the identification of rep

39 lated subcellular membranes, suggesting that tombusviruses have the ability to utilize alternative or

40 ribes the viral replication process based on tombusviruses, highlighting common strategies with other

41 ther, these advances in our understanding of tombusvirus-host interactions are broadly applicable to

42 ndrial CIRV, but not against the peroxisomal tombusviruses in yeast and plants.

43 ron microscopic imaging revealed the lack of tombusvirus-induced spherule-like structures in ESCRT-I

44 ing of vps23Delta yeast revealed the lack of tombusvirus-induced spherule-like structures, while cres

45 NA-dependent RNA polymerase preparation from tombusvirus-infected plants, revealed that this interact

46 ere miR168 is up-regulated, such as during a tombusvirus infection.

47 transgenic Nicotiana benthamiana plants from tombusvirus infections.

48 The p19 protein from the tombusvirus is such a viral suppressor of RNA silencing

49 necrosis virus (CNV), a member of the genus Tombusvirus, is transmitted in nature via zoospores of t

50 of Tomato bushy stunt virus (TBSV), a small tombusvirus of plants, we have developed a cell-free sys

51 of Tomato bushy stunt virus (TBSV), a small tombusvirus of plants, we used N-terminal truncated reco

52 ecrosis virus (CNV) is a member of the genus Tombusvirus, of which tomato bushy stunt virus (TBSV) is

53 mensional structure of an siRNA bound to the tombusvirus p19 protein--a suppressor of gene silencing-

54 Expression of the tombusvirus p33 replication protein in the absence of ot

55 The multifunctional tombusvirus p33 replication protein not only interacts w

56 inhibitory roles of various phospholipids in tombusvirus RdRp activation, we propose that the lipid c

57 Hsp70 function inhibits RNA synthesis by the tombusvirus RdRp in vitro.

58 Demonstration of the ability of carmo- and tombusvirus RdRps to switch RNA templates in vitro suppo

59 llular proteasomal protein, called Rpn11, in tombusvirus recombination in a yeast model host, in plan

60 This suggests that Rpn11p can suppress tombusvirus recombination via facilitating the recruitme

61 gether, the role of multiple RNA elements in tombusvirus replicase assembly could be an important fac

62 repRNA accumulation and the activity of the tombusvirus replicase by up to fivefold.

63 The tombusvirus replicase complex contains heat shock protei

64 and ESCRT-III proteins and the viral RNA in tombusvirus replicase complex formation using in vitro,

65 ement for these co-opted cellular factors in tombusvirus replicase formation.

66 w that the in vitro activity of the purified tombusvirus replicase from gef1Delta yeast was low and t

67 Altogether, tombusvirus replicase in the cell-free system showed fea

68 We found that the template activity of the tombusvirus replicase preparation was stimulated in tran

69 paper reports the first purified recombinant tombusvirus replicase showing high activity and template

70 s a decrease in the in vitro activity of the tombusvirus replicase when isolated from APB-treated yea

71 e viral replicase, (iii) the activity of the tombusvirus replicase, and (iv) the ratio of plus- versu

72 al RNA could not be used as templates by the tombusvirus replicase.

73 and degradation of proteasome substrates, in tombusvirus replication and recombination in Saccharomyc

74 und that the cellular Rpn11 is subverted for tombusvirus replication and Rpn11 has a proteasome-indep

75 eveloped ER and mitochondrion-based in vitro tombusvirus replication assays.

76 teins act as CIRFs and also as regulators of tombusvirus replication by inhibiting the assembly of ne

77 all, the current work provides evidence that tombusvirus replication could occur in vitro in isolated

78 re involved in determining the efficiency of tombusvirus replication in the two subcellular membranes

79 4), Vps20p, and Vps24p ESCRT-III proteins in tombusvirus replication in yeast and in vitro.

80 ligase and its WW domain in plant-infecting tombusvirus replication in yeast cells and in vitro usin

81 yeast cell extract and purified recombinant tombusvirus replication proteins to show that RII(+)-SL,

82 opsis homologs of Vps23p and Bro1p inhibited tombusvirus replication to greater extent than individua

83 n yeast or plant leaves led to inhibition of tombusvirus replication, confirming that CypA is a restr

84 our understanding of the role of sterols in tombusvirus replication, in this work we showed that the

85 ew focuses on virus-host interactions during tombusvirus replication.

86 a systematic deletion approach with a model tombusvirus replicon RNA in Saccharomyces cerevisiae, wh

87 d down or a mutated Rpn11 is expressed, then tombusvirus RNA goes through rapid viral recombination a

88 ast genes) that affected the accumulation of tombusvirus RNA.

89 A comparison of different tombusviruses showed, in each case, conservation for pot

90 By using tombusviruses, small model viruses of plants, we dissect

91 An emerging concept based on tombusviruses, small plant viruses, is that viruses migh

92 Previous studies with tomato bushy stunt tombusvirus (TBSV) in a yeast model host have revealed t

93 Previous work with Tomato bushy stunt tombusvirus (TBSV) in model host yeast has revealed esse

94 Tomato bushy stunt tombusvirus (TBSV) is a model virus that can replicate a

95 ave been found to inhibit Tomato bushy stunt tombusvirus (TBSV) replication in a Saccharomyces cerevi

96 Previous works with Tomato bushy stunt tombusvirus (TBSV) revealed the recruitment of either pe

97 een shown to affect RNA-RNA recombination in tombusviruses; this opens an opportunity to study the ro

98 d subcellular membranes might be utilized by tombusviruses to regulate new VRC assembly during the co

99 In this study, we used a plant RNA virus, tombusvirus, to examine the role of a cellular proteasom

100 Using the prototypical tombusvirus, Tomato bushy stunt virus (TBSV), we show th

101 me AMMECR1, the siRNA silencing repressor of tombusviruses, tRNA Wybutosine biosynthesis enzyme Tyw3p

102 ated with Tomato bushy stunt virus (TBSV), a tombusvirus, undergoes frequent recombination in plants

103 also functions in the proper assembly of the tombusvirus VRCs.

104 Replication of the RNA genomes of tombusviruses, which are small plus-sense RNA viruses of

105 oped an in vitro trans-replication assay for tombusviruses, which are small plus-strand RNA viruses.

106 Tomato bushy stunt virus (TBSV), a tombusvirus with a nonsegmented, plus-stranded RNA genom