ライフサイエンスコーパス: tonoplast

1 hat like CAX1, CAX3 is also localized to the tonoplast.

2 STAR1 form an ABC transporter complex in the tonoplast.

3 derived from the cell wall, cytomembrane and tonoplast.

4 ux of potassium salt at both plasmalemma and tonoplast.

5 ws that these dynamic networks represent the tonoplast.

6 Both are located in the tonoplast.

7 ion gradients across the plasma membrane and tonoplast.

8 protein), and show that it is located in the tonoplast.

9 molecules across its limiting membrane, the tonoplast.

10 ure, and subsequent vesicle docking with the tonoplast.

11 role in the transport of glucose across the tonoplast.

12 omised, GFP-KOR1 tended to accumulate at the tonoplast.

13 ions as a Fru-specific uniporter on the root tonoplast.

14 tack, trans-Golgi network/early endosome and tonoplast.

15 e lumen using the proton gradient across the tonoplast.

16 ms for newly synthesized transporters to the tonoplast.

17 oid bases (LCBs) and are associated with the tonoplast.

18 Intriguingly, tonoplast accumulation of BAK1 was substantially increas

19 SAC2-SAC5 localize to the tonoplast along with PtdIns3P, the presumable product of

20 Rerouted INT4 is functionally active in the tonoplast and complements the growth phenotype of an int

21 e membrane-associated and cofractionate with tonoplast and denser endomembrane markers in subcellular

22 the released energy to pump H(+) across the tonoplast and endomembranes to create proton motive forc

23 that an ABC protein, ABCC1, localizes to the tonoplast and is involved in the transport of glucosylat

24 K(+)-Na(+)/H(+) antiporter localized in the tonoplast and its gene expression is induced by salt, dr

25 -induced PCD, involving proteasome-dependent tonoplast and plasma membrane fusion followed by dischar

26 ordinate regulation of the exchangers in the tonoplast and plasma membrane.

27 in abundant levels of aquaporins in both the tonoplast and plasma membrane.

28 of major groups of transporters both at the tonoplast and plasma membrane.

29 Changes in the cytoskeleton, tonoplast and plastids also occur in the colonized cells

30 CROSE TRANSPORTER2 (SUC2), or SWEET1, to the tonoplast and that the position of the motif relative to

31 the preliminary localization of CAX1 to the tonoplast and the molecular and biochemical characteriza

32 porins are integral membrane proteins of the tonoplast and the plasma membrane that facilitate the pa

33 VCL1, AtVPS11, and AtVPS33 are found on the tonoplast and the prevacuolar compartment (PVC) by immun

34 idopsis thaliana inositol transporters INT1 (tonoplast) and INT4 (plasma membrane), we performed doma

35 uaporins are found in the vacuolar membrane (tonoplast) and the plasma membrane.

36 functionally akin to the vacuole of plants (tonoplast) and the small electron-dense granules of some

37 are major components of the plasma membrane, tonoplast, and other endomembranes of plant cells.

38 Colocalization with a tonoplast annexin VCaB42 shows that these dynamic networ

39 (Arabidopsis thaliana), the highly abundant tonoplast AQP isoforms AtTIP1;1, AtTIP1;2, and AtTIP2;1

40 es involved in cell elongation and division (tonoplast aquaporin and ubiquitin-like protein SMT3), ox

41 ZmTIP1 shows 76% sequence identity with the tonoplast aquaporin gamma-TIP (tonoplast intrinsic prote

42 ion to examine the expression pattern of the tonoplast aquaporin ZmTIP1 in different organs of maize

43 a previously characterized mercury-sensitive tonoplast aquaporin.

44 d group also has 11 members and contains the tonoplast aquaporins, and the third group has only a sin

45 hat clearly distinguish plasma membrane from tonoplast aquaporins.

46 g the targeting of these transporters to the tonoplast are largely unknown.

47 h (86)Rb(+) identified the efflux across the tonoplast as the sensitive process, implying that protei

48 ration is consistent with the existence of a tonoplast-bound transporter.

49 e an H(+) gradient preestablished across the tonoplast by either vacuolar H(+)-ATPase or vacuolar H(+

50 slocating ATPase activity, a 36% increase in tonoplast Ca(2+)-ATPase activity, and increased expressi

51 hat these mutants exhibit a 50% reduction in tonoplast Ca(2+)/H(+) antiport activity, a 40% reduction

52 Thus, the tonoplast can sense an osmotic gradient and respond to w

53 In plants, high capacity tonoplast cation/H(+) antiport is mediated in part by a

54 In plants, high capacity tonoplast cation/H(+) antiport is mediated in part by a

55 clude that the negative control of SV and FV tonoplast channel activity in old leaves reduces Na(+) l

56 low-activating (SV) and fast-activating (FV) tonoplast channels, linking it with Na(+) accumulation i

57 Subcellular localization to the tonoplast, complementation of a manganese (Mn)-sensitive

58 results demonstrate that the storage vacuole tonoplast contains delta-TIP, protein storage vacuoles c

59 The copper-induced increase in tonoplast CsHMA5.2 is consistent with the increased acti

60 asma membrane, select multivesicular bodies, tonoplast, dense intravacuolar bodies, and maturing meta

61 The absence of the Arabidopsis tonoplast Dicarboxylate Transporter (tDT) in the tdt kno

62 r, localization of CHS and CHI to the ER and tonoplast did not appear to be affected, suggesting that

63 phoproteomic analyses of rice shoot and root tonoplast-enriched and plasma membrane-enriched membrane

64 2+) accumulation and altered activity of the tonoplast-enriched Ca(2+)/H(+) antiporter.

65 CX1-expressing plants demonstrated increased tonoplast-enriched Ca2+/H+ antiport activity as well as

66 o ensure maximum coverage of the proteome, a tonoplast-enriched fraction was also analyzed separately

67 exchanger using quantitative proteomics of a tonoplast-enriched membrane fraction.

68 Uptake of (86)Rb(+) increased in tonoplast-enriched membranes isolated from Arabidopsis l

69 In contrast, H+ pumping by the fruit tonoplast-enriched membranes was chloride-independent, l

70 report high-capacity uptake of (86)Rb(+) in tonoplast-enriched vesicles from yeast expressing AtCCX3

71 idopsis TIP1;1 (gammaTIP) is a member of the tonoplast family of aquaporins (AQP).

72 are essential for active K(+) uptake at the tonoplast, for turgor regulation, and for stomatal funct

73 MIP-A and MIP-B were found in tonoplast fractions and also in fractions distinct from

74 We conclude that SlCAT9 is a tonoplast Glu/Asp/GABA exchanger that strongly influence

75 oform in Populus tremulaxalba, PtaSUT4, is a tonoplast (Group IV) symporter.

76 diating the NaCl-stress-induced increases in tonoplast H+-translocating ATPase (V-ATPase) and Na+/H+

77 After tonoplast implosion, XCP1 and XCP2 remained associated w

78 cuole before mega-autolysis was initiated by tonoplast implosion.

79 AMPA and NMG) across the plasma membrane and tonoplast in a manner characteristic of ATP-binding cass

80 f higher plant INT1-type transporters to the tonoplast in Arabidopsis mesophyll protoplasts.

81 iculum, Golgi apparatus, plasma membrane, or tonoplast in Arabidopsis plants; furthermore, based on b

82 oltage regulation of the plasma membrane and tonoplast in coordinating transport between the differen

83 d PIN3, both proteins were mis-sorted to the tonoplast in lip5 root cells.

84 as observed at the endoplasmic reticulum and tonoplast in these cells, and also in electron-dense reg

85 effector, HaRxL17, that associated with the tonoplast in uninfected cells and with membranes around

86 olved in membrane fusion on both the PVC and tonoplast in vivo.

87 characterized role in membrane fusion at the tonoplast in yeast.

88 rotein that regulates membrane fusion at the tonoplast in yeast.

89 The retention of plasma membrane and tonoplast integrity during cell shrinkage supports the i

90 vacuoles coincident with insertion of a new tonoplast intrinsic protein (TIP), delta-TIP, into their

91 es (PSV) are marked by the presence of alpha-tonoplast intrinsic protein (TIP), whereas lytic vacuole

92 ization of a green fluorescent protein-delta tonoplast intrinsic protein fusion.

93 af vacuoles expressing fluorescently labeled tonoplast intrinsic protein isoforms reveal an altered t

94 ntal and tissue-specific localization of two tonoplast intrinsic protein isoforms, the small leaf vac

95 ss AtTIP1;3 and AtTIP5;1, two members of the Tonoplast Intrinsic Protein subfamily of aquaporins.

96 tity with the tonoplast aquaporin gamma-TIP (tonoplast intrinsic protein) from Arabidopsis.

97 a new Arabidopsis aquaporin, delta-TIP (for tonoplast intrinsic protein), and show that it is locate

98 lant cells; thus, the tonoplast marker delta-tonoplast intrinsic protein-green fluorescent protein de

99 moves there directly from the ER; a specific tonoplast intrinsic protein; and a novel receptor-like R

100 Tonoplast intrinsic proteins (TIPs) have been implicated

101 Plant aquaporins are categorized as either tonoplast intrinsic proteins (TIPs) or plasma membrane i

102 and of membrane containing alpha- and delta-tonoplast intrinsic proteins (TIPs), markers for protein

103 : plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), nodulin 26-like int

104 use antipeptide antibodies specific for the tonoplast intrinsic proteins alpha-TIP, gamma-TIP, and d

105 demonstrate that antibodies to two different tonoplast intrinsic proteins, alpha-TIP and TIP-Ma27, la

106 uish plasma membrane intrinsic proteins from tonoplast intrinsic proteins.

107 igin and are not present in any of the known tonoplast intrinsic proteins.

108 rgely controlled by membrane channels called tonoplast-intrinsic aquaporins (TIP-AQPs).

109 A comparison of the NIPs and tonoplast-intrinsic proteins (TIP) shows that the H2 res

110 e that both INT1 and SUC4 trafficking to the tonoplast is sensitive to brefeldin A.

111 levels required for activation of different tonoplast K(+) channels.

112 f a SlCAT9-YFP fusion in tobacco confirmed a tonoplast localisation.

113 The tonoplast localization of AtTIP5;1 was established by re

114 , like its orthologs in other plant taxa, is tonoplast localized and thought to mediate Suc export fr

115 Here we present the discovery of a tonoplast localized nitrate/peptide family (NPF) transpo

116 Suc transport by the tonoplast localized SbSUT4 could not be detected using y

117 les destined for vacuolar sequestration by a tonoplast-localized ATP-binding cassette pump.

118 t-related targets of PH4, we silenced PH5, a tonoplast-localized H(+) -ATPase that maintains vacuolar

119 report that, in Arabidopsis guard cells, the tonoplast-localized K(+)/H(+) exchangers NHX1 and NHX2 a

120 Proteins NHX1 and NHX2 are the two major tonoplast-localized NHX isoforms.

121 Together, these results show that tonoplast-localized NHX proteins are essential for activ

122 Here, we present evidence of four tonoplast-localized soluble N-ethylmaleimide-sensitive f

123 Tonoplast-localized transporters catalyze the import and

124 -acylation in plants, and reveal a Golgi and tonoplast located S-acylation mechanism that affects a r

125 Membrane protein analysis confirmed MIP-F as tonoplast located.

126 A GFP-TIP1;1 fusion protein indicated tonoplast location in spongy mesophyll cells, and high s

127 most of the volume of plant cells; thus, the tonoplast marker delta-tonoplast intrinsic protein-green

128 A tonoplast marker, delta-TIP::GFP, under a pollen-specifi

129 conclude that this carrier is located on the tonoplast membrane and that it may mediate sugar partiti

130 trifugations showed that it co-migrates with tonoplast membrane markers.

131 retargeting of the aquaporin TIP1g from the tonoplast membrane to the symbiosome membrane.

132 tion chromatography of detergent-solubilized tonoplast membranes and was specifically cross-reactive

133 es, which are separated from the delineating tonoplast membranes by a layer of cytosolic material.

134 of the ER, generating a network of collapsed tonoplast membranes that are resorbed.

135 Vma6p homolog from red beet (Beta vulgaris) tonoplast membranes.

136 ) bidirectional fluxes across the plasma and tonoplast membranes.

137 ated C3G is transported to the vacuole via a tonoplast Mg-ATP-requiring glutathione pump (GS-X pump).

138 The tonoplast MIP-F was found in all cells, while signature

139 intrinsic protein isoforms reveal an altered tonoplast morphology resembling an amalgamation of a LV

140 Characterization of tonoplast Na+/H+ exchange demonstrated that it represent

141 These results demonstrate that (i) the tonoplast Na+/H+ exchanger in Arabidopsis is a target of

142 ivated SOS2 protein added in vitro increased tonoplast Na+/H+-exchange activity in vesicles isolated

143 When compared with tonoplast Na+/H+-exchange activity in wild type, activit

144 ults suggest that the dynamic Rop-containing tonoplast networks represent a unique stage of vacuole d

145 of other clades identify interactions at the tonoplast, nuclear membrane, and pollen tube plasma memb

146 s of OsCBL2 and OsCBL3 were localized to the tonoplast of aleurone cell protein storage vacuoles and

147 the presence of CsHMA5.1 and CsHMA5.2 in the tonoplast of cucumber cells.

148 of the Na(+)/H(+) antiporters acting at the tonoplast of E. californica cells mediates this proton f

149 imilar to AtNHX1, AtNHX2 is localized to the tonoplast of plant cells.

150 ted splice isoform, ZIFL1.3, localize to the tonoplast of root cells and the plasma membrane of leaf

151 rmeating species across both plasmalemma and tonoplast of root cells under toxicity conditions.

152 in the cortex of roots and localizes to the tonoplast of root cells.

153 ating bidirectional Fru transport across the tonoplast of roots in response to metabolic demand to ma

154 e ion channel predominantly expressed in the tonoplast of small vacuoles, we generated overexpressing

155 atiotemporal control of TIP abundance in the tonoplast of the different LRP cells is pivotal to media

156 ll configurations, using plasma membrane and tonoplast of three different species.

157 ns, but biochemical markers specific for the tonoplasts of functionally distinct vacuoles are poorly

158 e Na+/H+ exchanger in the vacuolar membrane (tonoplast) of Arabidopsis is also a target for the SOS r

159 d also in fractions distinct from either the tonoplast or PM.

160 oplasmic reticulum, the Golgi apparatus, the tonoplast, peroxisomes, mitochondria, plastids and the p

161 (MVB), transitory late endosome/ tonoplast, tonoplast, plastids, mitochondria, peroxisomes, autophag

162 kD polypeptide was dissociated from isolated tonoplast preparations by mild chaotropic agents and thu

163 psis thaliana) Ca(2+)/H(+) exchanger (sCAX1) tonoplast protein in tomato fruit on cellular Ca partiti

164 t that vacuole biogenesis and trafficking of tonoplast proteins and lipids can occur directly from th

165 the absence of two DUF300 domain-containing tonoplast proteins, LAZARUS1 (LAZ1) and LAZ1 HOMOLOG1 (L

166 the trafficking of sterols and of two major tonoplast proteins, the vacuolar H(+)-pyrophosphatase an

167 In the case of RB treatment, the tonoplast remained intact and no complex was formed.

168 est that as the LV transitions to a PSV, the tonoplast remodels before the large vacuole lumen is rep

169 are located in the plasma membrane (PM) and tonoplast, respectively.

170 membranes, including the plasma membrane and tonoplast, retained integrity.

171 Cathepsin B may execute its function after tonoplast rupture and works in parallel with VPE.

172 During and following cell shrinkage, tonoplast rupture did not occur, and membranes, includin

173 Tonoplast rupture was not altered in the cathepsin B mut

174 To study tonoplast rupture, a plant PCD feature, both confocal an

175 did not contain the protein storage vacuole tonoplast-specific protein alpha-TIP, and the sequestere

176 717-1B4) plants with reduced expression of a tonoplast sucrose efflux transporter, PtaSUT4, exhibit r

177 A hypothesis is advanced that SUT4-mediated tonoplast sucrose fluxes contribute to the regulation of

178 Trafficking of tonoplast-targeted proteins in infected symbiotic cells

179 uctance, and malic acid transport across the tonoplast) that are subject to feedback control (e.g. st

180 for transporters at the plasma membrane and tonoplast, the salient features of osmolite metabolism,

181 of ZmMRP3 in vivo shows its presence in the tonoplast, the site at which anthocyanin transport occur

182 er influx across the plasma membrane and the tonoplast to maintain adequate turgor pressure.

183 facilitates rapid flow of water through the tonoplast to permit osmotic equilibration between the cy

184 trate that CYBASC1 is localized at the plant tonoplast (TO).

185 ular bodies (MVB), transitory late endosome/ tonoplast, tonoplast, plastids, mitochondria, peroxisome

186 anion channels and H(+)-ATPase and with the tonoplast TPK K(+) channel.

187 a RING U-box protein and possible effect of tonoplast transport on ABA accumulation.

188 ripening, we hypothesised the existence of a tonoplast transporter that exports GABA from the vacuole

189 ening, the activation of plasma membrane and tonoplast transporters results in solute accumulation in

190 o types of vanadate-sensitive, ATP-dependent tonoplast transporters.

191 Lemon fruit tonoplasts, unlike those of seedling epicotyls, contain

192 reviously observed that the B subunit of the tonoplast V-ATPase is modified by the photoactivated nuc

193 )/H(+) antiport activity, a 40% reduction in tonoplast V-type H(+)-translocating ATPase activity, a 3

194 f plants to salt, the activities of both the tonoplast (vacuolar) H(+)-ATPase (V-ATPase) and Na+/H+ a

195 t property: transport of Glu across isolated tonoplast vesicle membranes was trans-stimulated in coun

196 nhydrovinblastine, was characterized using a tonoplast vesicle system.

197 the kinetics of ATP-driven proton pumping by tonoplast vesicles from lemon fruits and epicotyls were

198 Since the V-ATPase activity of native fruit tonoplast vesicles is insensitive to inhibitors, membran

199 ivity of ATP-dependent copper transport into tonoplast vesicles isolated from roots of plants grown u

200 ar level by reduced H+ transport activity of tonoplast vesicles isolated from sos2-2 cells relative t

201 Ion transport analyses with tonoplast vesicles isolated from transgenic lines confir

202 Tonoplast vesicles isolated from transgenic plants showe

203 Cd(2+) and Mn(2+) transport in isolated root tonoplast vesicles.

204 of the Na(+),K(+)/H(+) antiport activity in tonoplast vesicles.

205 d aquaporin fusion protein TIP1;1-YFP to the tonoplast was blocked (leading to its accumulation in th

206 Again, the efflux of K(Rb) at the tonoplast was the sensitive process.

207 This tonoplast water channel is highly expressed in the root

208 fluorescent protein fusions localized to the tonoplast, which engulfs the major sugar storage compart

209 mbranes upon infection, in particular to the tonoplast, which was located close to the extra-haustori

210 complex, INT1 is correctly localized to the tonoplast, while sorting of the vacuolar sucrose transpo