ライフサイエンスコーパス: topoisomerase III

1 sors is strictly dependent on topB (encoding topoisomerase III).

2 uld explain its functional coordination with topoisomerase III.

3 e IIIalpha and Schizosaccharomyces pombe DNA topoisomerase III.

4 possess topoisomerase IB appear to lack DNA topoisomerase III.

5 We cloned cDNA encoding Drosophila DNA topoisomerase III.

6 ifth was sufficient for interaction with DNA topoisomerase III.

7 the protein it encodes has been denoted DNA topoisomerase III.

8 However, topoisomerase III alpha and Bloom helicase can dissolve

9 ses: reverse gyrase and a protein complex of topoisomerase III alpha and Bloom helicase.

10 larger protein complex ('BLMcx') containing topoisomerase III alpha, RMI1, RMI2 and replication prot

11 ad low amino acid sequence similarity to DNA topoisomerase III, an enzyme that relaxes DNA supercoils

12 s human BLM and yeast Sgs1 interact with DNA topoisomerase III and are thought to act on stalled repl

13 ginally identified in S.cerevisiae ) between topoisomerase III and DNA helicases of the RecQ family,

14 Rqh1 forms a complex with topoisomerase III and is proposed to process or disrupt

15 YjhX inhibits only topoisomerase I but not topoisomerase III and IV in vitro.

16 nlinking, we demonstrated directly that both topoisomerase III and topoisomerase IV were efficient at

17 PG0104, which is highly similar (57%) to DNA topoisomerase III, and PG0121, which has high similarity

18 ge site sequence analysis suggest that it is topoisomerase III, and therefore we named it "NeqTop3."

19 RecQ helicases and topoisomerase III are both required for genome stability

20 Helicases of the RecQ family and topoisomerase III are evolutionarily conserved proteins

21 Overexpression of topoisomerase III at the nonpermissive temperature was s

22 Escherichia coli DNA topoisomerase III belongs to the type IA family of DNA t

23 tween the appearance of chromosome-bound DNA topoisomerase III beta and Rad51, a protein known to be

24 es of wild-type mice also indicates that DNA topoisomerase III beta becomes prominently associated wi

25 hromosomal defects in germ cells lacking DNA topoisomerase III beta, and this interpretation is suppo

26 ion of the mouse TOP3 beta gene encoding DNA topoisomerase III beta, one of the two mammalian type IA

27 Additionally, the expression of topoisomerase III beta, which exhibited a clear pattern

28 lved crystal structures of wild-type E. coli topoisomerase III bound to an eight-base ssDNA molecule

29 d 33% sequence identity to Bacillus subtilis topoisomerase III (bsTopo III) and Escherichia coli topo

30 g the putative DNA binding cavity of E. coli topoisomerase III, creating a molecule that can be coval

31 merase III (bsTopo III) and Escherichia coli topoisomerase III (ecTopo III) respectively.

32 In budding yeast, loss of topoisomerase III, encoded by the TOP3 gene, leads to a

33 out physiological functions associated with topoisomerase III enzyme in other bacteria.

34 ficant sequence similarity to genes encoding topoisomerase III enzymes in other eukaryotic species.

35 RecQ-like DNA helicases pair with cognate topoisomerase III enzymes to function in the maintenance

36 protein is a member of the beta-subfamily of topoisomerase III enzymes.

37 hly conserved CXXC motifs not found in other topoisomerase III enzymes.

38 DNA topoisomerase III from Escherichia coli belongs to the t

39 The topoisomerase III gene ( top3 (+)) from Schizosaccharomy

40 ination of gene structure for a second human topoisomerase III gene.

41 The removal of topoisomerase III has no effect on the accumulation of c

42 A human homolog of topoisomerase III has previously been identified.

43 In sgs1 top3 cells devoid of DNA topoisomerase III, however, expression of full-length Sg

44 lution, of an inactive mutant of E. coli DNA topoisomerase III in a non-covalent complex with an 8-ba

45 These data are consistent with a role for topoisomerase III in disentangling recombination interme

46 omerase III/IV mutant, indicating a role for topoisomerase III in recombination.

47 nce points to the role of RecQ helicases and topoisomerase III in regulating homologous recombination

48 nd a bias against deleting the gene encoding topoisomerase III in ruvC53 or DeltaruvABC backgrounds c

49 We show here that the removal of topoisomerase III in temperature sensitive topoisomerase

50 dies on the interaction between Sgs1 and DNA topoisomerase III in vitro and in vivo.

51 ies can be catalyzed by Escherichia coli DNA topoisomerase III, indicating that this enzyme can act a

52 mbination, including the helicase domain and topoisomerase III interaction domain, are essential.

53 Topoisomerase III interacts with DNA helicase SGS1 and t

54 Whereas topoisomerase III is a more efficient decatenase than to

55 which the association of a RecQ helicase and topoisomerase III is important for facilitating decatena

56 The presence of more than one human topoisomerase III is reminiscent of mammalian topoisomer

57 The physiological role of topoisomerase III is unclear for any organism.

58 Because the DNA topoisomerase III isozymes are likely to interact with h

59 rome genes are likely to act in concert with topoisomerase III isozymes in human cells.

60 mice points to the possibility that the DNA topoisomerase III isozymes might be involved in the path

61 ion of ruvABC allows the construction of the topoisomerase III/IV double mutant.

62 escued the synthetic lethality of the double topoisomerase III/IV mutant, indicating a role for topoi

63 We postulate that topoisomerase III may be deregulated in A-T cells and th

64 type IA topoisomerases, including mammalian topoisomerase III, may also help link the enzyme activit

65 lly rescue the slow growth defect of a yeast topoisomerase III mutant.

66 resolution reaction is specific for the RecQ-topoisomerase III pair and is mediated by interaction of

67 e III; therefore, these results suggest that topoisomerase III participates in orderly and efficient

68 and in vivo data indicate that Sgs1 and DNA topoisomerase III physically interact and that this inte

69 Thus, mechanisms by which RecQ helicases and topoisomerase III proteins cooperate to maintain genomic

70 RecQ helicases and topoisomerase III proteins interact physically and funct

71 The cellular function of Escherichia coli topoisomerase III remains elusive.

72 eukaryotes are well established, the role of topoisomerase III remains poorly defined.

73 hromosome maintenance, including p53, BRCA1, topoisomerase III, replication protein A and DNA polymer

74 Shorter pauses for topoisomerase III result in a higher decatenation rate.

75 cerevisiae, loss of the TOP3 gene, encoding topoisomerase III, results in a phenotype of slow growth

76 The topoisomerase III RuvC double mutants that can be constr

77 In addition, topoisomerase III shows a strong dependence on the cross

78 IV and is unlikely to define a new role for topoisomerase III; therefore, these results suggest that

79 ll-length ORF for hTOP3, the first mammalian topoisomerase III to be identified.

80 residues 1-283, inhibited the binding of DNA topoisomerase III to single-stranded DNA.

81 uman BLM and yeast Sgs1, form a complex with topoisomerase III (Top3) and are thought to act during D

82 B activity prevents the proper regulation of topoisomerase III (Top3) function, disrupting a recombin

83 mportant and has been implicated in Sgs1-DNA topoisomerase III (Top3) interaction.

84 The topoisomerase III (Top3)-Rmi1 heterodimer, which catalyz

85 DNA helicases are known to interact with DNA topoisomerase III (Top3).

86 Together, RecQ helicase and topoisomerase III (Topo III) of Escherichia coli compris

87 has been identified in Escherichia coli DNA topoisomerase III (Topo III) that is essential for Topo

88 his activity specifically stimulates E. coli topoisomerase III (Topo III) to fully catenate dsDNA mol

89 ogy simplification activity, and for E. coli topoisomerase III (topo III), a type IA topoisomerase th

90 Escherichia coli topB, the gene encoding DNA topoisomerase III (Topo III).

91 The largest gene product of the novel topoisomerase III was expressed and shown to interact wi

92 Eukaryotic DNA topoisomerase III was first identified by studying the h

93 topB, encoding topoisomerase III, was identified as a high copy suppres

94 Using Escherichia coli RecQ and topoisomerase III, we demonstrate a second activity for

95 DNA-bound, catalytically inactive mutant of topoisomerase III where DNA binding realigns catalytic r