ライフサイエンスコーパス: total RNA

1 tide specificity, and processes only 5 ng of total RNA.

2 mining only polyadenylated RNA and the other total RNA.

3 d well with profiles representing undepleted total RNA.

4 A libraries from a minute amount (500 pg) of total RNA.

5 tion of global microRNA (miRNA) abundance in total RNA.

6 leted of ribosomal RNA from only 1 microg of total RNA.

7 iological stains using as little as 50 pg of total RNA.

8 itative method for single-cell sequencing of total RNA.

9 on average once every 35 uridine residues in total RNA.

10 collagen was examined by real-time RT-PCR on total RNA.

11 , H. pylori produced abundant ppGpp and less total RNA.

12 within a range of 5 ng to 50 pg of starting total RNA.

13 ion, microarray analysis was also done using total RNA.

14 confirmed by primer extension sequencing of total RNA.

15 in vitro transcripts were spiked with plant total RNA.

16 ty of sample types including human serum and total RNA.

17 protocol requires a minimum of 20 microg of total RNA.

18 MMP-2 was examined by real-time RT-PCR with total RNA.

19 nscriptome sequencing compared to 21% in the total RNA.

20 s verified by reverse transcription-PCR with total RNA.

21 methods require 5-40 microg of high-quality total RNA.

22 xon junction in complementary DNA from blood total RNA.

23 tect most isoacceptors from minute amount of total RNA.

24 sativa L.) by high-throughput sequencing of total RNA.

25 erformed tunable hybrid capture of mRNA from total RNA.

26 lightly higher (by 1.6-fold) in mRNA than in total RNA.

27 P-dependent increase in rRNAs but not in the total RNA 24 h after 5-HT treatment.

28 core, surgical, and LCM specimen, sufficient total RNA (3 to 6 microg) was extracted for cDNA array a

29 , cDNA probes can be prepared with much less total RNA (5 microg or less) without amplification produ

30 This protocol uses as little as 10 ng of total RNA, allows multiplex sequencing of up to 96 sampl

31 We have developed a total RNA amplification and labeling strategy for use wi

32 ned by RT-PCR of human TM (HTM) cell-derived total RNA and by PCR amplification of HTM cell-derived a

33 Total RNA and double-stranded RNA (dsRNA) from mycelia a

34 milar expression profiles were obtained with total RNA and enriched small RNA species (R(2) >or= 0.97

35 Total RNA and immunoprecipitated dsRNA from Escherichia

36 nanoliter volumes, using sequence from bulk total RNA and multiplexed quantitative PCR as benchmarks

37 We found that the total RNA and protein content, as well as the size of th

38 C activation has also been shown to increase total RNA and protein production in many tissue and dise

39 han afforded by the analysis of steady-state total RNA and should be useful in many biological settin

40 erase chain reaction signals per nanogram of total RNA and using NucleoSpin and mirVana columns is pr

41 synthetic mixtures of human genomic DNA and total RNA and with total RNA isolated from cells.

42 Protein, total RNA, and DNA were extracted from postmortem tempor

43 CR and Western blotting were performed using total RNA, and protein extracted from mouse CECs and hum

44 Genomic DNA, total RNA, and protein were isolated from mouse tissues

45 R gene expression with as little as 1 mug of total RNA, and using it, we profiled three human bone ma

46 ed PA sorbent extracted sufficient mRNA from total RNA at concentrations as low as 5 ng muL(-1) in aq

47 ve amounts of messenger RNA as a fraction of total RNA between the two independent samples.

48 studies using a titration of mouse universal total RNA, BIIB outperformed commercially available kits

49 developed a method of isolating dsRNAs from total RNA by immunoprecipitation with a ds-RNA specific

50 eling and detection of small RNAs present in total RNA by splinted ligation.

51 nriched preparations were then obtained from total RNA by subtracting eucaryotic ribosomal and messen

52 at which activity was detected and increased total RNA cleavage at high Mg(2+) concentrations suffici

53 ibits a linear signal across a wide range of total RNA concentrations and agrees well with standard c

54 acutely increases polyribosome occupancy of total RNA, consistent with an increase in mRNA translati

55 rial, methods were developed to: (1) isolate total RNA containing amplifiable mRNA from human skin an

56 ession (-64 +/- 5% vs. IGF-1; P < 0.001) and total RNA content (-16 +/- 2% vs. IGF-1; P < 0.001) in I

57 was associated with significant increases in total RNA content and protein metabolism.

58 ate with differences in proteasome activity, total RNA content, mRNA content, or cell division rate.

59 se transcription-PCR using G. sulfurreducens total RNA demonstrated that the genes encoding these thr

60 d be reisolated in the cDNA pool or from the total RNA derived from the same or a different tissue, i

61 Total RNA derived from these cells of different confluen

62 ted with milk appears to be the isolation of total RNA directly from SC or MFG released into milk dur

63 ng the protocol for a commercially available total RNA/DNA extraction kit (RecoverALL).

64 a decreased capacity for protein synthesis (total RNA/DNA) and decreased sensitivity and capacity of

65 ed using both methods from as little as 5 ng total RNA (~equivalent to 2 x 105 bacilli).

66 iked into hundreds of nanograms of the plant total RNA extract with a recovery below 110% using eithe

67 recently been assessed via pyrosequencing of total RNA extracted directly from natural microbial asse

68 eous detection of three endogenous miRNAs in total RNA extracted from 293T and HeLa cells.

69 med a whole-genome microarray analysis using total RNA extracted from actively growing broth cultures

70 ng circulating miRNAs in serum and miRNAs in total RNA extracted from cancer cells.

71 y is conducted on the detection of miRNAs in total RNA extracted from cultured cells.

72 of circulating miRNAs in blood and miRNAs in total RNA extracted from cultured cells.

73 was applied for determination of miR-221 in total RNA extracted from human lung and breast cancer ce

74 Analysis of total RNA extracted from M. catarrhalis ATCC 43617 cells

75 separate short RNA libraries generated from total RNA extracted from M. truncatula leaves, represent

76 Total RNA extracted from plasma-derived EXOs of 12 T1DM

77 Total RNA extracted from the P7 lenses of Rho GDIalpha t

78 l-time reverse transcription-PCR analysis of total RNA extracted from the parental strain and csrA(Bb

79 eaction (RT-PCR) analysis of genomic DNA and total RNA extracted from the same sample before and afte

80 and cervical lymph nodes were harvested for total RNA extraction and gene expression by RT and real-

81 miRNA following DNA extraction as opposed to total RNA extraction for both blood- and saliva-specific

82 Endometrial biopsies were taken and total RNA extraction, cDNA synthesis and PCR was perform

83 supernatant of ocular fluid was subjected to total RNA extraction, followed by complementary deoxyrib

84 The cells were subjected to total RNA extraction, reverse transcription (RT), and re

85 fied the differential expression of miR21 in total RNA extracts from healthy breast tissue and diseas

86 trating expression profiling capabilities in total RNA extracts from the HL-60 cell line.

87 s can determine relative levels of miRNAs in total RNA extracts with sensitivity similar to small RNA

88 estimate the copy number range of miRNAs in total RNA extracts.

89 chieved routinely with as little as 25 pg of total RNA for most miRNAs.

90 tely collected, can be used as the source of total RNA for QRT-PCR analysis.

91 hod includes a novel treatment that depletes total RNA fractions of highly abundant tRNAs and small s

92 reference sample consisting of a mixture of total RNA from 10 different normal human tissues not inc

93 To this end, we sequenced total RNA from 18 psoriasis patients and 16 healthy cont

94 rons are equivalent populations, we isolated total RNA from 25 Fluoro-Jade-positive and 25 Newport Gr

95 Total RNA from 27 fresh tumor samples of 15 solid/multic

96 Initially, total RNA from 4 tissues samples per group was employed

97 Total RNA from 5000 cells was SMART amplified, [33]P-lab

98 The authors extracted total RNA from 60-day, 80-day, and 96-day human fetal re

99 Total RNA from 61 SCC samples and 10 matched normal lung

100 nt in total RNA from C. elegans and pRNAs in total RNA from bacteria.

101 method has become widely used for isolating total RNA from biological samples of different sources.

102 Total RNA from blood was processed on human Affymetrix m

103 de and can detect specific miRNAs present in total RNA from C. elegans and pRNAs in total RNA from ba

104 eveloped a method for preparing high-quality total RNA from Ca-alginate-encapsulated Saccharomyces ce

105 The quality of total RNA from CECs was assessed by a bioanalyzer and RT

106 d miR-203 and miR-21 in samples of extracted total RNA from cell cultures.

107 Total RNA from cultured cementoblasts treated with 5 mM

108 Total RNA from dermal fibroblasts of 15 discordant twin

109 sisRNA is detectable by RT-PCR in samples of total RNA from embryos up to the mid-blastula stage, whe

110 Moreover, total RNA from Escherichia coli, depleted of rRNA, also

111 iption-polymerase chain reaction of isolated total RNA from ethidium-bromide-treated and untreated ce

112 suitable for isolating high yield and clean total RNA from field-grown plants.

113 Expression of miRNAs was determined using total RNA from formalin-fixed, paraffin-embedded tissue

114 Subsequent quantitative analysis of total RNA from higher organisms revealed varying levels

115 Total RNA from HMCs at 0, 2, 4, and 6 h was extracted, l

116 In vitro, total RNA from hMSCs was extracted one week after cultur

117 Examination of total RNA from human cytomegalovirus-infected cells demo

118 RNase R digestion of the total RNA from human skeletal muscle generates an RNA po

119 his approach was applied to the detection of total RNA from human tissues and found to display differ

120 Total RNA from kidney biopsies was isolated at 3 clinica

121 Total RNA from lungs, hearts, kidneys, livers, and splee

122 se DNA microarrays were then used to analyze total RNA from M. catarrhalis cells grown in a continuou

123 RT-PCR of total RNA from M. nemestrina and Macaca fascicularis yie

124 Up to 75% of the total RNA from mullet ovaries was 5S rRNA.

125 e was identified by 5' RACE techniques using total RNA from NIH 3T3 cells.

126 1.2-kb cpb2-specific mRNA was identified in total RNA from our surveyed isolates.

127 Total RNA from ovaries of drought-stressed wild-type and

128 ene expression profiling was performed using total RNA from peripheral blood mononuclear cells (PBMCs

129 Total RNA from peripheral blood mononuclear cells from 5

130 sine kinase receptor EphA4 were expressed in total RNA from Sezary cells and the paired amplified mRN

131 Expression profiling of total RNA from ten normal bone cell lines and eleven OGS

132 Northern blot analysis of total RNA from the O46E parent strain revealed a readily

133 Total RNA from the retina/(retinal pigment epithelium) w

134 With total RNA from the retina/(retinal pigment epithelium, R

135 le or no uspA2H transcript was detectable in total RNA from the UspA2H-negative variant O46E.U2V.

136 An RNA isolation kit was used to isolate total RNA from tissue samples that had been stabilized i

137 Total RNA from TM was used for quantitative PCR, while p

138 RT-PCR was performed on total RNA from wild-type mouse retina to identify the Dy

139 To define their compartmentalization, total RNA >100 nt was extracted from sonicated (SS) mous

140 e oligonucleotides have been synthesized and total RNA has been extracted, the procedure can be compl

141 ption (RT)-PCR and Northern blotting of lens total RNA, immunoblotting of lens membrane extracts, and

142 lemented and evaluated; one was added to the total RNA in the samples before amplification and labeli

143 icable to most qRT-PCR assays performed with total RNA, independent of the expression levels of the g

144 RNA-seq performed on total RNA indicated that only a fraction of total miRNAs

145 st one and up to four orders of magnitude of total RNA input and independent of sample composition.

146 t four targets, and requires just 10.3 ng of total RNA input in a 2 hour and 15 minutes assay.

147 sion profiles were generated with 100-200 ng total RNA input.

148 Two hundred picograms of total RNA is found to be sufficient for this analysis.

149 For DNA microarray analysis, total RNA is reverse-transcribed, labeled by incorporati

150 Total RNA is then recovered by precipitation with isopro

151 e method can be performed on crude lysate or total RNA, is fast, highly reproducible and minor change

152 olymerase chain reaction were performed with total RNA isolated from blood cells of kidney graft reci

153 Using total RNA isolated from both E. coli and NTHi, we show f

154 Meanwhile, gene profiles using total RNA isolated from bulk tumors of the same 28 patie

155 of human genomic DNA and total RNA and with total RNA isolated from cells.

156 rse transcription of the mRNA portion of the total RNA isolated from each sample.

157 In an important validation study, total RNA isolated from four major chicken tissues: ceca

158 antly lower (by 6.5-43-fold) in mRNA than in total RNA isolated from HEK293T cells, whereas the level

159 miRNA microarray analyses of the hippocampal total RNA isolated from mice chronically treated with mu

160 ted nucleobase products (m(5)C and m(6)A) in total RNA isolated from mouse brain, pancreas, and splee

161 ic separation of picogram-nanogram levels of total RNA isolated from multiple cell types, including t

162 Transcriptome sequencing of total RNA isolated from Tat- and vehicle-treated periphe

163 Total RNA isolated from whole blood of 26 un-medicated T

164 Total RNAs isolated from renal proximal tubules (RPTs) o

165 immunoprecipitation (IP), (ii) extracted for total RNA isolation for Northern blotting and, (iii) ana

166 was further improved by a modified method of total RNA isolation from serum/plasma, S/P miRsol, in wh

167 Data generated using the total RNA kit had more signal for introns and various RN

168 ols (ERCs), which can be either added to the total RNA level (tERCs) or introduced right before hybri

169 During meiosis, total RNA levels parallel 3' processing, which occurs in

170 5 NHP species and subspecies and constructed total RNA libraries for the same approximately 15 tissue

171 strategy for detecting 16S rRNA sequences in total RNA mixed samples extracted from the three pathoge

172 eply profile lncRNAs from polyadenylated and total RNA obtained from human neocortex at different sta

173 ps, the expression of 22000 transcripts from total RNA of frozen tumour samples from 286 lymph-node-n

174 Gene expression was assessed on the total RNA of induced sputum (n = 83), nasal brushings (n

175 through 5-hydroxymethylcytidine (hm(5)C) in total RNA of mammalian cells.

176 We labeled total RNA of MEG-01 cells by incorporation of 5-ethynylu

177 The total RNA of the lacrimal gland was then extracted, at e

178 mixing using the same 10 microg of starting total RNA on 22 000-probe arrays, 10 000 more usable sig

179 time PCR from cDNA generated from intestinal total RNA or from RNA obtained by laser capture microdis

180 copies as the outcome variable and the input total RNA or plasma volume as an exposure variable, whic

181 in the presence of a million-fold excess of total RNA, paving the way for simple, point-of-care, low

182 which we extracted an average of 14.1 pg of total RNA per cell.

183 binding to DNA constrained by the values of total RNA polymerase (E) and sigma(70) per cell measured

184 nd a promoter complex with reduced levels of total RNA polymerase II (Pol II) and Pol II phosphorylat

185 e RNA purification steps could influence the total RNA pool, we examined the impact of RNA isolation,

186 ype, transcript abundance in the nuclear and total RNA pools are highly correlated; whereas, in attpr

187 ated by RT-PCR and Northern blot analysis of total RNA preparations.

188 acids in abundant levels of genomic DNA and total RNA, processing of various sample types, and carry

189 transcripts accounted for up to one-third of total RNA reads from the infected-cell RNA population.

190 Under acidic conditions, total RNA remains in the upper aqueous phase, while most

191 and 44% present calls from 100 and 50 pg of total RNA, respectively.

192 Northern blots and RT-PCR analysis of total RNA revealed truncated transcripts that suggested

193 MiRNA-93 and miRNA-223 were determined by total RNA reverse transcription.

194 oxorubicin and performed RNA sequencing from total RNA (RNA-Seq) and AGO2-immunoprecipitated RNA (AGO

195 obtain quantitative information relating to total RNA, RNA subunits, and undermodified nucleosides i

196 ogenous levels of the mature target miRNA in total RNA (RNAt) extracted from cancerous and non-cancer

197 the endogenous levels of both target miRs in total RNA (RNAt) extracted from metastatic breast cancer

198 monstrated by determining mature miRNA-21 in total RNA (RNAt) extracted from tumor cells and human ti

199 ple, mRNA expression analysis performed on a total RNA sample isolated from a biological stain may be

200 Attempts were made in profiling miRNAs in total RNA samples extracted from cancer cells and blood.

201 nt microarrays carrying tens of thousands of total RNA samples in the MAQC project on the study of re

202 Total RNA samples were collected at 1, 3, 6, 9, and 24 h

203 llows the reproducible profiling of up to 24 total RNA samples within 24 h.

204 on of ten transcripts in two different human total RNA samples, we find good agreement between our si

205 ermination of target miRNA spiked into human total RNA samples.

206 ermination of target miRNA spiked into human total RNA samples.

207 Coupling total RNA-Seq and transcriptome re-assembly we annotated

208 Computationally identifying circRNAs from total RNA-seq data is a primary step in studying their e

209 The analyzed extensive set of total RNA-Seq data, together with the improvement of the

210 itive approach for predicting circRNAs using total RNA-seq data.

211 ipeline named UROBORUS to detect circRNAs in total RNA-seq data.

212 Total RNA-seq profiling of young (1 month old) and aged

213 etect circRNAs with low expression levels in total RNA-seq without RNase R treatment.

214 Using total RNA-Seq, we have found that transcription in 1-cel

215 in 130 introns of 115 Drosophila genes from total RNA sequencing data generated from developmental t

216 We performed sRNA and total RNA sequencing from control and abiotically stress

217 Using total RNA sequencing, we found progressive disruption of

218 Total RNA-sequencing (RNA-seq) and comparative transcrip

219 mans with cocaine use disorder, we performed total RNA-sequencing on neuronal nuclei isolated from po

220 DNA hypermethylation and with a decrease in total RNA synthesis.

221 get preparation (by reverse transcription of total RNA), target-probe hybridization on array, signal

222 racting 16 microg of mRNA from 315 microg of total RNA, the 0.4-microL volume monolith showed no sign

223 croRNA complement and sequenced libraries of total RNA to investigate the relationships with microRNA

224 ome analysis require a large amount of input total RNA to yield sufficient mRNA using either poly-A s

225 Here, we separately sequenced total RNAs (Total RNAseq) and mRNAs (mRNAseq) from the s

226 fied biosensing of mir21 from cell lysate of total RNA was demonstrated.

227 Total RNA was extracted and analyzed via quantitative (r

228 Total RNA was extracted and mRNA expression was analyzed

229 rols, and 22 rheumatic disease controls, and total RNA was extracted and reverse transcribed into com

230 blood was obtained before and after CRT, and total RNA was extracted for hybridization-based whole ge

231 Total RNA was extracted for microarray and real-time RT-

232 Total RNA was extracted from 335 serum samples of HIV pa

233 Total RNA was extracted from cultures treated for 6 hour

234 Total RNA was extracted from exosomes purified from 152

235 Total RNA was extracted from formalin fixed paraffin emb

236 Total RNA was extracted from gingival biopsies from 26 p

237 Total RNA was extracted from gingival biopsy samples col

238 Total RNA was extracted from gingival tissue biopsies co

239 Total RNA was extracted from ileal biopsy specimens and

240 Total RNA was extracted from Sezary cells and amplified

241 Total RNA was extracted from the cells at baseline (cont

242 Next, total RNA was extracted from the tissue and analyzed wit

243 ion and purified by centrifugation, and then total RNA was extracted using hot acid phenol.

244 From the peripheral blood cells, total RNA was extracted, quantified, and used for one-st

245 Efficient isolation of eukaryotic mRNA from total RNA was first mathematically modeled and then achi

246 Total RNA was harvested from the aortic rings, and repor

247 Labeled cRNA derived from TG-isolated total RNA was hybridized to 430 2.0 chips containing 14,

248 The resultant labeled cRNA from TG isolated total RNA was hybridized to gene microarray chips contai

249 Total RNA was isolated and gene expression levels were d

250 At designated times, total RNA was isolated and gene expression was measured

251 Total RNA was isolated and in vitro transcribed and hybr

252 Total RNA was isolated and reverse-transcribed.

253 Total RNA was isolated and used as a template in quantit

254 Total RNA was isolated at different time points followin

255 For expression studies, total RNA was isolated from 168 ileal biopsies of study

256 Total RNA was isolated from adipose tissue and adiponect

257 Total RNA was isolated from cell cultures and subjected

258 Total RNA was isolated from cells treated for 6 to 24 ho

259 Total RNA was isolated from early-passage fibroblasts ob

260 To analyze integrin expression, total RNA was isolated from experimental and control cel

261 Total RNA was isolated from four biological replicates o

262 Total RNA was isolated from IL-1beta-induced and mock-in

263 Total RNA was isolated from KB cells 1 hour after treatm

264 Total RNA was isolated from lymphoblastoid cell lines es

265 Total RNA was isolated from microdissected dentate gyrus

266 Total RNA was isolated from PBMCs of five children with

267 Total RNA was isolated from pooled human TM cell monolay

268 Total RNA was isolated from raft cultures and used to ge

269 Total RNA was isolated from sorted fractions and used fo

270 Total RNA was isolated from the four treatment groups in

271 Total RNA was isolated from the human limbus and central

272 Total RNA was isolated from the MSCs on day 3.

273 Total RNA was isolated from TM cell strains, and mRNA ex

274 Total RNA was isolated from TM of cadaveric eyes derived

275 Total RNA was isolated from whole lung 24 and 48 h postf

276 Total RNA was isolated systematically throughout the int

277 Total RNA was isolated using TRIzol reagent from 42 appe

278 Total RNA was isolated, and qualitative RT-PCR was perfo

279 Total RNA was isolated, and qualitative RT-PCR was perfo

280 weeks, and 8 weeks after each treatment, and total RNA was isolated.

281 and 8-week-old mice were microdissected, and total RNA was isolated.

282 Glands were collected, and total RNA was isolated.

283 male BALB/c mice (n = 5/sex/experiment), and total RNA was isolated.

284 Total RNA was prepared using TRIzol reagent.

285 lens were dissected from each rat's eye, and total RNA was purified.

286 The isolated total RNA was then subjected to gene expression profilin

287 Total RNA were extracted and profiled on Affymetrix mous

288 Microbiota DNA and host total RNA were isolated from 189 bronchoalveolar lavages

289 Microbiota DNA and host total RNA were isolated from 203 bronchoalveolar lavages

290 Total RNAs were extracted followed by microarray hybridi

291 The total RNAs were isolated and subjected to semiquantitati

292 Total RNAs were isolated for reverse transcription- quan

293 e background (5,000 to 10,000 copies per mug total RNA) were detected.

294 and mitochondrial RNA, but not by mammalian total RNA, which is abundant in modified nucleosides.

295 processed for histology or the isolation of total RNA, which was analyzed for differentially express

296 he detection limit was found to be 0.4 ng of total RNA, which was much lower than that obtained using

297 ary preparation kits (standard poly-A versus total RNA with Ribozero depletion) and analysis pipeline

298 pC-mt4 led to an increase in the recovery of total RNA with time in contrast to TA toxins that inhibi

299 tle as 10 pg of mRNA ( approximately 1 ng of total RNA) with this approach.

300 l RNAs from nanogram to microgram amounts of total RNA without an amplification step.