ライフサイエンスコーパス: trans-splicing

1 other than chromosomal rearrangement (e.g., trans-splicing).

2 partners of the split intein (called protein trans-splicing).

3 ions in polycistronic RNA precursors through trans splicing.

4 -3 and -4 can lead to a 20-fold decrease in trans splicing.

5 ligo-U 3' extension that is removed prior to trans splicing.

6 SR proteins in splice site communication in trans splicing.

7 e of the third stem-loop plays a key role in trans splicing.

8 ther changes result in a substantial loss of trans splicing.

9 imary sequence of stem II is crucial for SL2 trans splicing.

10 nucleotide substitution in the loop prevents trans splicing.

11 of C. elegans and other species that use SL2 trans-splicing.

12 reversing the roles of snRNP and pre-mRNA in trans-splicing.

13 transcriptome of C. elegans for patterns of trans-splicing.

14 (m(2,2,7)G) cap derived from spliced leader trans-splicing.

15 g elements and could favor cis-splicing over trans-splicing.

16 n the phenomenon of spliceosome-mediated RNA trans-splicing.

17 plicing reactions is essential for effective trans-splicing.

18 c screen and shown to be defective for rps12 trans-splicing.

19 st a framework for DNA recombination and RNA trans-splicing.

20 to Igmu/BCL6 CT and were not generated from trans-splicing.

21 rapy could be overcome by pre-mRNA segmental trans-splicing.

22 nown as exon scrambling, exon repetition and trans-splicing.

23 ed an essential function at an early step in trans-splicing.

24 anistic link between cap 4 modifications and trans-splicing.

25 15 ESTs representing five types of putative trans-splicing.

26 in organisms featuring a cap 4 structure and trans-splicing.

27 e natural regulation of CD40L using pre-mRNA trans-splicing.

28 ith SL1 RNA and related SmY RNAs, impairs SL trans-splicing.

29 otein assembly, have an important role in SL trans-splicing.

30 ct migrated to the chloroplast and underwent trans-splicing.

31 in ligated aadA-smGFP due to In-Ic-mediated trans-splicing.

32 phyla involves spliced leader (SL) addition trans-splicing.

33 may involve the production of joint RNAs by trans-splicing.

34 identified that are involved uniquely in SL trans-splicing.

35 ive splicing, and (4) multiple promoters and trans-splicing.

36 t mRNAs in Hydra receive leader sequences by trans-splicing.

37 of a complex containing CstF-64, but not for trans-splicing.

38 quence is added to the 5'end of each mRNA by trans-splicing.

39 hotosystem I subunit A involves two steps of trans-splicing.

40 transcripts into close proximity to promote trans-splicing.

41 proteins known to be involved in nematode SL trans-splicing.

42 at the 5' terminus that usually accompanies trans-splicing.

43 n, including polycistronic transcription and trans-splicing.

44 he intron underwent a transition from cis to trans splicing 15 times among the sampled angiosperms.

45 In trans-splicing, a common exon, the spliced leader, is ad

46 mall viral packaging capacity of the vector, trans-splicing AAV vectors are able to package twice the

47 Furthermore, the trans-splicing activity of the Ssp DnaE intein was succe

48 Spliced leader (SL) RNA trans splicing adds a trimethylguanosine (TMG) cap and a

49 encing led to a block of spliced leader (SL) trans splicing, an essential step in trypanosome mRNA ma

50 bstitution in the absence of ZnCl(2) blocked trans splicing and decreased C-terminal cleavage kinetic

51 ximately 20-bp element is sufficient for SL2 trans splicing and mRNA accumulation when transplanted t

52 However, recent discoveries of trans-splicing and cis-splicing events between neighbori

53 nding of these factors to pre-mRNA regulates trans-splicing and hence expression of specific genes.

54 nciples and latest work in bifunctional RNA, trans-splicing and modification of U1 and U7 snRNA to ta

55 and long non-coding RNAs (lncRNAs), undergo trans-splicing and polyadenylation.

56 polyadenylate non-coding RNAs, which undergo trans-splicing and polyadenylation.

57 ia genes and detected widespread alternative trans-splicing and polyadenylation.

58 hat are dissected by the concerted action of trans-splicing and polyadenylation.

59 fs in the untranslated regions regulate mRNA trans-splicing and RNA stability, yet where UTRs begin a

60 p codons, as well as high levels of editing, trans-splicing and the addition of oligonucleotide caps

61 ed through the discovery of highly efficient trans-splicing and trans-cleaving inteins.

62 aturation in Trypanosoma brucei depends upon trans splicing, and variations in trans-splicing efficie

63 logy, including polycistronic transcription, trans-splicing, and a glycosome-like organelle.

64 As: lariat introns, Y-structure introns from trans-splicing, and circular exons through exon skipping

65 ed mutants in sna-1 that are defective in SL trans-splicing, and demonstrate that reducing function o

66 in vivo evidence for proteins involved in SL trans-splicing, and indicate that continuous replacement

67 ost protein-coding transcripts is removed by trans-splicing, and noncoding initiation sites have not

68 f gene pieces, joining of precursor RNAs via trans-splicing, and RNA editing by substitution and urid

69 element expansion, horizontal gene transfer, trans-splicing, and simplification of gene structure and

70 ering more efficient vectors for dual-vector trans-splicing approaches.

71 Here, we successfully demonstrate RNA trans-splicing as an auspicious repair option for mutati

72 antibodies, "Bispecific Antibody by Protein Trans-splicing (BAPTS)".

73 Thus, the trans-splicing between CoAA and RBM4 transcripts may rep

74 d that the switched alternative splicing and trans-splicing between CoAA and RBM4 transcripts result

75 er, identify 80 genes that appear to undergo trans-splicing between homologous alleles and can be cla

76 Our results suggest that trans-splicing between homologous alleles occurs more co

77 ears to arise from physiologically regulated trans-splicing between precursor messenger RNAs for JAZF

78 It is exceedingly rare to have trans-splicing between protein-coding exons and has been

79 which exon repetition occurs as a result of trans-splicing between separate pre-mRNA transcripts fro

80 tamers in a head-to-tail orientation through trans-splicing between these two independent vector geno

81 on in the mod(mdg4) gene is a consequence of trans-splicing between two different mutant transcripts.

82 elivered transgenes with this vector through trans-splicing between two independent vectors coadminis

83 ch served as templates for mapping the first trans-splicing branch points in T. cruzi.

84 However, efficient trans splicing can be restored by inserting a second AG

85 Trans-splicing/cleavage reactions were initiated by comb

86 The characterization of this psaA trans-splicing complex is also of interest from an evolu

87 d, including inter-chromosome translocation, trans-splicing, complex chromosomal rearrangements, and

88 Spliced leader (SL) RNA trans-splicing contributes the 5' termini to mRNAs in a

89 In conclusion, RNA trans-splicing could provide the basis of therapeutic st

90 We have used an in vitro trans-splicing/cross-linking system in Saccharomyces cer

91 ual-vector sets had a significantly enhanced trans-splicing efficiency (6- to 10-fold, depending on t

92 pends upon trans splicing, and variations in trans-splicing efficiency could be an important step in

93 Y) tract with cytidine resulted in increased trans-splicing efficiency, whereas purines led to a larg

94 t form, with only a relatively small loss in trans-splicing efficiency.

95 independent ESTs represent the same putative trans-splicing event.

96 formed by either fusion genes (DNA level) or trans-splicing events (RNA level).

97 he first insights into tRNA accumulation and trans-splicing events in higher plant plastids.

98 The trans-splicing events uncovered in Austrobaileyales, eum

99 ghput approaches cannot distinguish cis- and trans-splicing events, the extent to which trans-splicin

100 Its maturation requires two cis- and two trans-splicing events.

101 s splicing demonstrate that PPR4 is an rps12 trans-splicing factor.

102 the feasibility of spliceosome-mediated RNA trans-splicing for imaging gene expression at the level

103 t displayed splicing activity similar to the trans-splicing form, which suggests that the N- and C-te

104 e or decrease in E10 content was achieved by trans-splicing from a target carrying the Delta280K muta

105 precursor spliced leader RNA was tested for trans-splicing function in vivo by mutating the intron.

106 ion at the 3' ends of the upstream genes and trans splicing, generally to the specialized spliced lea

107 Metazoan spliced leader (SL) trans-splicing generates mRNAs with an m(2,2,7)G-cap and

108 As model system, a trans-splicing group I intron ribozyme was evolved in Es

109 SL trans-splicing has been documented in a limited but dive

110 een downstream location in an operon and SL2 trans-splicing has been strong, but anecdotal.

111 Despite this, the precise details of SL trans-splicing have yet to be elucidated.

112 ates that both proteins are essential for SL trans-splicing; however, neither protein is required eit

113 f the Ssp DnaE intein with ZnCl(2) inhibited trans splicing, hydrolysis-mediated N-terminal trans cle

114 analyze sequence requirements for efficient trans splicing in the poly(Y) tract and around the 3'SS,

115 wn to mediate efficient in vivo and in vitro trans-splicing in a foreign protein context.

116 es its ability to screen for rare intragenic trans-splicing in any target gene with a large backgroun

117 Spliced leader (SL) trans-splicing in Caenorhabditis elegans attaches a 22-n

118 report the detection of spliced leader (SL) trans-splicing in calanoid copepods.

119 e yeast cells by using intracellular protein trans-splicing in combination with a highly efficient sp

120 We uncovered the SL trans-splicing in copepod natural populations, and demon

121 ncing of mRNA to identify genes that undergo trans-splicing in Drosophila interspecies hybrids.

122 biological significance and evolution of SL trans-splicing in eukaryotes.

123 ubstrate spliced leader RNA transcription or trans-splicing in Leishmania tarentolae.

124 FP-based reporter assay that can monitor SL1 trans-splicing in living C. elegans.

125 y much higher than for examples of bona fide trans-splicing in mammals.

126 vel developmental paradigms for the study of trans-splicing in metazoans.

127 ive effect of a downstream 5' splice site on trans-splicing in nematode extracts containing either in

128 Here, we demonstrate intein trans-splicing in Nicotiana tabacum chloroplasts by usin

129 te that several inteins can catalyze protein trans-splicing in tens of seconds rather than hours, as

130 Trans-splicing in the Drosophila melanogaster modifier o

131 ork for the coupling of 3' end formation and trans-splicing in the processing of polycistronic pre-mR

132 NP onto the branch site as well as efficient trans-splicing in these inactive extracts could be rescu

133 Trans-splicing in trypanosomes and nematodes has been ch

134 Trans-splicing in trypanosomes involves the addition of

135 g that U1 recruitment is critical to promote trans-splicing in vivo.

136 , mRNAs are processed by spliced leader (SL) trans splicing, in which a capped SL, derived from SL RN

137 hese findings represent a novel mechanism of trans-splicing, in which RNA motifs in the 5' intron are

138 A naturally occurring trans-splicing intein from the dnaE gene of Synechocysti

139 splicing were investigated by employing the trans-splicing intein from the dnaE gene of Synechocysti

140 T7 endonuclease I was produced by means of a trans-splicing intein system.

141 Trans-splicing introduces a common 5' 22-nucleotide sequ

142 Protein trans-splicing involving naturally or artificially split

143 Thus E10 inclusion can be modulated by trans-splicing irrespective of the strength of the cis-s

144 Spliced leader (SL) trans-splicing is a biological phenomenon, common among

145 Spliced leader (SL) trans-splicing is a critical element of gene expression

146 It has also been suggested that trans-splicing is a mechanism for the generation of chim

147 Spliced leader trans-splicing is an mRNA maturation process used by a s

148 trans-Splicing is essential for mRNA maturation in trypa

149 e, a potentially ubiquitous reaction such as trans-splicing is not consistent with a phenomenon that

150 R proteins and suggest that plastid group II trans-splicing is performed by different machineries in

151 another function of flatworm spliced leader trans-splicing is to provide some recipient mRNAs with a

152 The chemical mechanism of protein trans-splicing is well-understood and has been exploited

153 Unlike typical cis-splicing, trans-splicing joins exons from two separate transcripts

154 Dual trans-splicing lacZ vectors were used to functionally ev

155 ctures and encode transcripts that display a trans splicing leader at the 5' end.

156 Thus, HIGM1 can be corrected by CD40L trans-splicing, leading to functional correction of the

157 de the first in vivo demonstration that SMN2 trans-splicing leads to a lessening of the severity of t

158 ito lays in a single strand, suggesting that trans-splicing may have originated in the Drosophila lin

159 The use of protein trans-splicing may help facilitate exciting new avenues

160 Alternative cis- or trans-splicing may overcome the need for genomic structu

161 dings provide new opportunities to study how trans-splicing may regulate the phenotype of a cell.

162 xon of a splicing substrate, apparently by a trans splicing mechanism.

163 hereby signifying an intein-mediated protein trans-splicing mechanism reconstituted in plant cells.

164 It is capable of catalyzing a protein trans-splicing mechanism to assemble a mature protein fr

165 These transcripts arise by both cis- and trans-splicing mechanisms, are expressed in a tissue-spe

166 and wild-type muscles, a comparable level of trans-splicing-mediated beta-galactosidase expression wa

167 AAV concatemers may have contributed to the trans-splicing-mediated transgene expression.

168 ask basic questions about RNA catalysis, the trans-splicing model system will also facilitate the dev

169 A pre-trans-splicing molecule (PTM) corrected endogenous FVIII

170 nactivating (SIN) lentiviral vector a 3' RNA trans-splicing molecule, capable of replacing COL7A1 exo

171 nature of the sequences delivered by the pre-trans-splicing molecule.

172 Pre-trans-splicing molecules (PTMs) were engineered to capit

173 Trans-splicing molecules designed to increase exon 10 in

174 Because of the modular design of pre-trans-splicing molecules, there is great potential to em

175 frameshift was required for splicing or that trans splicing occurred.

176 Trans-splicing occurred maximally at pH 7.0, while a sli

177 Trans-splicing occurred whether both intein fragments we

178 d trans-splicing events, the extent to which trans-splicing occurs between protein-coding exons in an

179 SL2 trans-splicing occurs in order to separate the products

180 functional mRNAs in a process that requires trans splicing of a capped 39-nucleotide RNA derived fro

181 nating its 5'-terminal region in SL addition trans splicing of nuclear pre-mRNA.

182 In all trypanosomatids, trans splicing of the spliced leader (SL) RNA is a requi

183 onventional cis-splicing or for bimolecular (trans-) splicing of fragmented cis constructs.

184 Studies of trans-splicing of 5' exon mimics to a truncated rRNA pre

185 Through trans-splicing of a 39-nt spliced leader (SL) onto each

186 The cap 4 becomes part of the mRNA through trans-splicing of a 39-nucleotide-long sequence donated

187 pm(6,6)AmpAmpCmpm(3)Ump-SL RNA) conveyed via trans-splicing of a universal spliced leader.

188 omplexes (I and II) that are responsible for trans-splicing of either psaA-i1 or psaA-i2.

189 Trans-splicing of eri-6/7 is mediated by a direct repeat

190 presence of genes arranged in tandem arrays, trans-splicing of messenger RNAs, and a reduced role for

191 of the last 5' intron, TSA and TSB, promote trans-splicing of mod(mdg4).

192 Trans-splicing of one of two short leader RNAs, SL1 or S

193 chimeric gene products normally generated by trans-splicing of RNAs in developing tissues.

194 the existence of chimeric RNAs generated by trans-splicing of RNAs transcribed from distant genomic

195 In kinetoplastid flagellates trans-splicing of spliced leader (SL) to polycistronic p

196 trypanosomatid protozoa is mediated through trans-splicing of the capped spliced leader (SL) sequenc

197 mation of the upstream mRNA and SL2-specific trans-splicing of the downstream mRNA.

198 n of mouse derived peptides, suggesting that trans-splicing of the knock-in human cDNA with the endog

199 We show that EMB2654 is required for the trans-splicing of the plastid rps12 transcript and that

200 t (RNA maturation of psaA 7) is deficient in trans-splicing of the second intron of psaA, and may be

201 of a multimeric complex that is required for trans-splicing of the second intron of psaA.

202 Therefore, trans-splicing of the SL1 leader sequence may serve at l

203 I intron ribozymes have been used to mediate trans-splicing of therapeutically relevant RNA transcrip

204 ons, compensatory changes in U1 snRNA rescue trans-splicing of TSA mutants, demonstrating that U1 rec

205 methylguanosine cap structure as a result of trans-splicing onto the 5' end of the pre-mRNA.

206 idine did not increase the rate or extent of trans-splicing or cleavage under typical assay condition

207 elations between stage-specific preferential trans-splicing or polyadenylation sites and differential

208 r gamma or mutant beta-globin gene addition, trans-splicing or RNA interference.

209 Coupled with experimental examination of trans-splicing patterns, our comparative genomic analysi

210 ggest that operons and "spliced leader" (SL) trans-splicing predate the radiation of the nematode phy

211 NA-seq by taking advantage of the endogenous trans-splicing process.

212 The 'trans-splicing' process in lower eukaryotes is well unde

213 The protein trans-splicing (PTS) activity of naturally split inteins

214 Expressed protein ligation (EPL) and protein trans-splicing (PTS) are both intein-based approaches th

215 Protein trans-splicing (PTS) by split inteins has found widespre

216 olypeptides through a process termed protein trans-splicing (PTS).

217 ications on cellular chromatin using protein trans-splicing (PTS).

218 ith serine decreased N-terminal cleavage and trans-splicing rates; however, this substitution did not

219 nes have 5' ends, they receive their caps by trans splicing rather than by capping enzymes.

220 SMaRT creates a hybrid mRNA through a trans-splicing reaction between an endogenous target pre

221 ell-to-cell variability in ribozyme-mediated trans-splicing reaction efficiency.

222 l be applicable in any organisms that have a trans-splicing reaction from spliced leader RNA.

223 This trans-splicing reaction has ATP, Mg(2+), and splice-site

224 he ACT1-CUP1 reporter gene, resulting from a trans-splicing reaction in which a 5' splice site-like s

225 The trans-splicing reaction involves the association of 5' a

226 fragments in the hinge region by the protein trans-splicing reaction of a split intein to generate Bs

227 conclusion from this observation is that the trans-splicing reaction that takes place between transcr

228 ng these building blocks initiates an intein trans-splicing reaction that yields a hydrogel that is h

229 ranscription units that can be combined in a trans-splicing reaction to form the mature Mod(mdg4)2.2-

230 The trans-splicing reaction was partially blocked at a conce

231 Efficiency and specificity of the trans-splicing reaction were found to vary depending on

232 genic plants for reconstitution of a protein trans-splicing reaction.

233 are required for at least two stages of the trans-splicing reaction.

234 ent of SL ribonucleoproteins consumed during trans-splicing reactions is essential for effective tran

235 cubation of complementary pairs, we observed trans-splicing reactions with unprecedented rates and yi

236 ssembly of three distinct precursor RNAs via trans-splicing reactions, and the accumulation of nad5T1

237 ut suggest that apparent exon scrambling and trans-splicing result, respectively, from in vitro artif

238 Tau RNA trans-splicing resulted in an increase in exon 10 inclus

239 Trans-splicing ribozymes are RNA-based catalysts capable

240 established to select efficient and specific trans-splicing ribozymes from a ribozyme library.

241 We describe an alternative strategy with trans-splicing ribozymes that can simultaneously reduce

242 Based on this ability of trans-splicing ribozymes to deliver new gene activities,

243 Trans-splicing ribozymes were used to splice sequences e

244 tem that involved the coexpression of a SMN2 trans-splicing RNA and an antisense RNA that blocks a do

245 ween an endogenous target pre-mRNA and a pre-trans-splicing RNA molecule (PTM).

246 Here, we evaluated the potential of trans-splicing RNA reprogramming to correct tau mis-spli

247 In SL trans-splicing, SL-donor transcripts (SL RNAs) contain t

248 reprogramming using spliceosome-mediated RNA trans-splicing (SMaRT) could be a method of choice to co

249 Spliceosome-mediated RNA trans-splicing (SMaRT) provides an effective means to re

250 We carried out spliceosome-mediated RNA trans-splicing (SMaRT) to repair mutant FVIII mRNA.

251 Spliceosome-mediated RNA trans-splicing (SMaRT) was investigated as a means for f

252 uld be modulated by spliceosome-mediated RNA trans-splicing (SMaRT).

253 RNP proteins function by participating in a trans-splicing specific network of protein protein inter

254 anscription of SLRNA genes, which encode the trans splicing-specific spliced leader RNA, suggests tha

255 The nematode SL is derived from a trans-splicing-specific approximately 100-nucleotide RNA

256 within the polycistronic pre-mRNA, and that trans-splicing specificity is recapitulated in vitro.

257 by a gene from another organism, no loss of trans-splicing specificity was observed, suggesting that

258 We examined the cis- vs. trans-splicing status of the mitochondrial group II intr

259 Here, we addressed this issue using a trans-splicing strategy that allows modulating tau exon

260 We employed pre-mRNA segmental trans-splicing (STS), in which two engineered DNA fragme

261 ated synthesis of spliced leader (SL) RNA, a trans splicing substrate and key molecule in trypanosome

262 The SL RNA is a small nuclear RNA and a trans splicing substrate for the maturation of all pre-m

263 e assay based on competition between cis-and trans-splicing suggested that factors in addition to U1

264 his information was used to develop a tandem trans-splicing system based on native and engineered spl

265 we reported the development of an optimized trans-splicing system that involved the coexpression of

266 erty allowed the development of an on-column trans-splicing system that permitted the facile separati

267 An in vitro trans-splicing system was developed that used a bacteria

268 We demonstrate that the RNA trans-splicing technology combined with a SIN lentiviral

269 replaces the mutant-containing 3' portion by trans-splicing the corresponding 4-kb wild-type sequence

270 We show that due to alternative trans splicing, the human pathogen Leishmania naturally

271 the joining mechanism possibly involves tRNA trans-splicing, the presence of an intron might have bee

272 We define an RNA sequence required for SL2 trans-splicing, the U-rich (Ur) element, through mutatio

273 Although it is sometimes assumed to arise by trans-splicing, there is no evidence of this and the eff

274 spliced leader RNA and joined to pre-mRNA by trans-splicing, thus providing mature mRNAs with an m7G

275 tion was also determined to be necessary for trans splicing to proceed on a substrate that had U2 snR

276 h Zn(2+), reactants can be diverted from the trans splicing to the trans cleavage pathway where DTT a

277 Finally, increased use of SL1 trans-splicing to downstream operon genes can indicate t

278 ammable DNA-binding protein by using protein trans-splicing to ligate synthetic elements to a nucleas

279 non-covalent fragments of the ribozyme allow trans-splicing to occur in both a reverse splicing assay

280 ugh the cap 4 modifications are required for trans-splicing to occur, the underlying mechanism remain

281 These findings add trans-splicing to the list of RNA-related functions asso

282 f nascent transcripts by polyadenylation and trans-splicing, together with specific rates of mRNA tur

283 spliced to both, indicating that SL1 and SL2 trans-splicing use different underlying mechanisms.

284 ternative splicing, and it differed from the trans splicing used in nematode operons.

285 Protein trans-splicing using split inteins is well established a

286 This occurs by 3' end formation and trans-splicing using the specialized SL2 small nuclear r

287 trate that in vivo delivery of the optimized trans-splicing vector increases an important SMN-depende

288 were compared to homologous AV5:5 and AV2:2 trans-splicing vector sets for their ability to reconsti

289 e splitting site has a profound influence on trans-splicing vector-mediated gene expression.

290 to-head fashion, we generated two hybrid ITR trans-splicing vectors (AV5:2LacZdonor and AV2:5LacZacce

291 as a guide, we generated a set of efficient trans-splicing vectors and achieved widespread expressio

292 Unfortunately, the efficiency of current trans-splicing vectors is very low.

293 This demonstrates the use of trans-splicing vectors to efficiently express a large th

294 These hybrid trans-splicing vectors were compared to homologous AV5:5

295 Specific trans-splicing was observed in living animals (P = 0.086

296 In searching for examples of trans-splicing, we looked only at reproducible events wh

297 -terminal cleavage, C-terminal cleavage, and trans-splicing were (1.0 +/- 0.5) x 10(-3), (1.9 +/- 0.9

298 ivation energies for N-terminal cleavage and trans-splicing were determined by Arrhenius plots to be

299 species and is essential and sufficient for trans-splicing, which binds U1 small nuclear RNP (snRNP)

300 ressed does the split intein mediate protein trans-splicing, yielding a full-length T7 RNA polymerase