ライフサイエンスコーパス: transamidation

1 of epsilon(gamma-glutamyl)lysine crosslinks (transamidation).

2 ne, and that the cells thus are defective in transamidation.

3 the kinetics of tRNA(Gln) glutamylation nor transamidation.

4 amide metathesis reactions that proceed via transamidation.

5 (< or =20 mol %) results in highly selective transamidation.

6 glutamyl isomers as intermediate products of transamidation.

7 ether to form in high yields the products of transamidation.

8 ed high energy intermediate in Glu-tRNA(Gln) transamidation.

9 t glutamine hydrolysis is coupled tightly to transamidation.

10 s and aminolysis reactions during productive transamidation.

11 the protein in the endoplasmic reticulum by transamidation, a reaction in which a C-terminal GPI-att

12 Although the transamidation active site is similar to those of other

13 in cells exhibited enhanced GTP binding and transamidation activities and mimicked the survival adva

14 Moreover, both Gln-tRNA and Asn-tRNA transamidation activities are present.

15 I), which is capable of both GTP binding and transamidation activities, has been implicated in a vari

16 IL-1 beta and ATRA induced TG transamidation activity and calcification in wild-type b

17 activity of TGase-S is not dependent on its transamidation activity because the mutation of a cystei

18 drolyze GTP to GDP; binding GTP inhibits its transamidation activity but allows it to function in sig

19 uctural basis for the negative regulation of transamidation activity by bound nucleotide, and the pos

20 Moreover, excessive transamidation activity did not appear to be detrimental

21 trate that the Ca(2+)-mediated regulation of transamidation activity is essential for the ability of

22 CXCR2 ligand GROalpha also induced increased transamidation activity of chondrocyte transglutaminases

23 ) from misacylated Glu-tRNA(Gln) through the transamidation activity of Glu-tRNA(Gln) amidotransferas

24 le inhibitors were identified that block the transamidation activity of hTG2 and allosterically aboli

25 When assayed in vitro, the transamidation activity of TGase-II is Ca(2+)-dependent.

26 Recent studies have suggested that the transamidation activity of TGase-II is necessary for the

27 in a manner that does not require either TG2 transamidation activity or fibronectin binding.

28 is primarily known for its calcium-dependent transamidation activity that leads to formation of an is

29 ersible, noncompetitive inhibitor of TGase 3 transamidation activity, similar to GTPgammaS and GDP.

30 In addition to its transamidation activity, TGase can bind guanosine 5'-tri

31 a2+ concentration reciprocally regulate TG's transamidation activity, with nucleotide binding being t

32 the Ca(2+)-mediated regulation of TGase-II's transamidation activity, with our goals being to identif

33 three sites inhibited the Ca(2+)-responsive transamidation activity.

34 nding/hydrolytic capability and an enzymatic transamidation activity.

35 yme that couples an ability to bind GTP with transamidation activity.

36 retains a wild-type level of NH(3)-dependent transamidation activity.

37 d expression/activation of TGase and in vivo transamidation and activation of RhoA.

38 ability of TGase to bind GTP and/or catalyze transamidation and found that GTP binding was sufficient

39 nctional protein with reciprocally regulated transamidation and purine nucleotide-binding activities,

40 ations to the plasma membrane by controlling transamidation and Rab4-GTP formation.

41 rt, formation, or usage in processes such as transamidation and translation.

42 Because bacteria primarily use transamidation, and the eukaryal cytoplasm uses glutamin

43 Competitive amidine formation and transamidation are observed with Cp*TiIV complexes, gene

44 minum(III) dimer Al2(NMe2)6 catalyzes facile transamidation between simple secondary carboxamides and

45 do not inhibit but instead activate TGase 3 transamidation by about 10-fold.

46 dation, intermolecular transpeptidation, and transamidation by added nucleophile.

47 al tail of FN are known to be susceptible to transamidation by both TG2 and activated blood coagulati

48 d nucleotide, and the positive regulation of transamidation by Ca2+.

49 ctivation of small GTPase RhoA via enzymatic transamidation by cytoplasmic tTG.

50 ases, substrate binding, Gln hydrolysis, and transamidation by Glu-AdT are tightly coordinated.

51 We conclude that transamidation by TG2 transforms S100A11 into a covalent

52 Transamidation catalytic activity of TG2 was not require

53 eover, S100A11 is covalently cross-linked by transamidation catalyzed by transglutaminase 2 (TG2), it

54 2 release modulates tissue repair, partly by transamidation-catalyzed covalent crosslinking of extrac

55 lymerizations, wherein side reactions (e.g., transamidation) compete with chain propagation, resultin

56 a nucleotide-bound closed conformation or a transamidation-competent open conformation.

57 ) and Gln-competitive, inhibition under full transamidation conditions mirrors the coupling between G

58 ertiary amides, which arises from successive transamidation cycles.

59 uble-mutants that were both GTP-binding- and transamidation-defective also stimulated cell death.

60 overexpression of wild-type tTG, but not its transamidation-defective counterpart, fully mimics the g

61 Microinjection of Rb(-/-) cells with a transamidation-defective TGase mutant and Rb afforded no

62 cts Rb from caspase-induced degradation in a transamidation-dependent manner.

63 different outcomes, amidine formation versus transamidation, depending on the identity of the TiIV co

64 on solution directly, as such intramolecular transamidation has been dramatically suppressed via kine

65 of primary amide substrates, secondary amide transamidation has remained elusive.

66 and catalyzes the cross-linking of proteins (transamidation), has been implicated both in the promoti

67 itu and, without isolation, are subjected to transamidation in the presence of zirconium chloride (0.

68 ation reactions and previously characterized transamidations involving secondary amides and primary a

69 Transamidation is a post-translational modification of p

70 We hypothesized that protein transamidation is essential for MB formation.

71 n which its catalytic domain is exposed, but transamidation is not needed for the interaction.

72 This shows for the first time that transamidation is not only stabilizing the skin and the

73 This demonstrates that transamidation is the only pathway to Gln-tRNAGln in B.

74 silient fertilization envelope: proteolysis, transamidation, NADPH-dependent oxidation and peroxidati

75 ransglutaminase (TG2, which is implicated in transamidation of 5-HT to Rac1) are observed in the mous

76 ulation of cells with high [5HT](ex) induces transamidation of a small GTPase, Rab4.

77 The scope of Lewis acid-catalyzed transamidation of acylated lactams was explored through

78 ge of amines in good to excellent yield, via transamidation of dimethylformamide.

79 anism we determined for gammaGTase-catalyzed transamidation of gammaGlu-AMC by Gly-Gly to form gammaG

80 uncompetitive inhibitor of Gly-Gly-promoted transamidation of gammaGlu-AMC.

81 glutaminylation of tRNAGln but by a specific transamidation of Glu-tRNAGln.

82 The alternative route involves transamidation of incorrectly charged tRNA.

83 of correctly charged Gln-tRNAGln through the transamidation of misacylated Glu-tRNAGln, functionally

84 de aminoacyl-tRNAs, Asn-tRNA or Gln-tRNA, by transamidation of mischarged Asp-tRNA(Asn) or Glu-tRNA(G

85 (iii) Studies of the transamidation of N,N-dimethylated casein by Gly-OMe and

86 e advances have been made with regard to the transamidation of primary amide substrates, secondary am

87 Two protocols for the transamidation of primary amides with primary and second

88 The activation of TGase results in increased transamidation of RhoA, which is inhibited by monodansyl

89 ling, which involves activation of TGase and transamidation of RhoA.

90 The highly efficient transamidation of several primary, secondary, and tertia

91 osttranslational modification of proteins by transamidation of specific polypeptide-bound glutamine r

92 Me2)6, promote facile equilibrium-controlled transamidation of tertiary carboxamides with secondary a

93 processed GPI-anchored proteins and identify transamidation of the GPI signal sequence as a step in P

94 of this study include: (i) gpTGase-catalyzed transamidation of Z-Gln-Gly by Gly-OMe proceeds essentia

95 by LipL, which catalyses the amidotransfer (transamidation) of the octanoyl moiety from octanoyl-Gcv

96 Transglutaminase 2 (TG2) catalyzes transamidation or deamidation of its substrates and is o

97 oming the classic problem of secondary amide transamidation, our studies expand the growing repertoir

98 We wondered whether a similar transamidation pathway also operates in the formation of

99 ctrum of bacteria rely on the tRNA-dependent transamidation pathway as the sole route to asparagine.

100 We demonstrate that an entire transamidation pathway composed of aspartyl-tRNA synthet

101 rly work suggested that mitochondria use the transamidation pathway for Gln-tRNA formation.

102 most surprising of these findings has been a transamidation pathway for the synthesis of asparaginyl-

103 the operon for the essential tRNA-dependent transamidation pathway harbors a SL with two potential o

104 Thus, the transamidation pathway operates only for Gin-tRNAGln for

105 cteria, archaea, and chloroplasts employ the transamidation pathway, in which a tRNA-dependent glutam

106 Instead, Gln-tRNA is formed via the transamidation pathway, the other route to this essentia

107 -tRNA(Asn) amidotransferase, employed by the transamidation pathway.

108 is made from aspartate in the tRNA-dependent transamidation pathway.

109 e expression and amino acid biosynthesis via transamidation pathways.

110 In a number of cases, transamidation proceeds rapidly at room temperature.

111 PCY1 S9A protease fold has been adapted for transamidation, rather than hydrolysis, of acyl-enzyme i

112 r parameters relating to the kinetics of the transamidation reaction in the context of rough microsom

113 the endoplasmic reticulum and consists of a transamidation reaction in which fully assembled GPI anc

114 oring of cell surface proteins consists of a transamidation reaction in which preassembled GPI donors

115 the proof that GPI anchoring proceeds via a transamidation reaction mechanism.

116 on of the lactam, and a lanthanide-catalyzed transamidation reaction of the Fmoc-protected lactam, us

117 proposed mechanism of the GPI transfer is a transamidation reaction that involves the formation of a

118 erazine unit occurring via an intermolecular transamidation reaction under mild condition, resulting

119 id requirements at the COOH terminus for the transamidation reaction using HDZ as the nucleophilic ac

120 of these amino acids as amide donors in the transamidation reaction.

121 ynthetic chemistry is the development of the transamidation reaction.

122 chanistic differences between these tertiary transamidation reactions and previously characterized tr

123 ficient catalysts for equilibrium-controlled transamidation reactions between secondary amines and te

124 ons and previous mechanistic suggestions for transamidation reactions catalyzed by transition metals

125 However, the transamidation route to Gln-tRNA formation is idled by t

126 y on glutamines at either end of (1-5)F1 and transamidation specificity of both enzymes is achieved t

127 uble point mutant (K3R/Q102N) of S100A11 TG2 transamidation substrate sites.

128 In one protocol for transamidation, the N'-acyl-N,N-dialkylformamidine inter

129 to contemporary cells, or cenancestor, used transamidation to synthesize Gln-tRNA(Gln) and that both

130 t that the ability of TGase to modify Rb via transamidation underlies the ability of TGase to provide

131 es and amines are found to undergo efficient transamidation using the methods described.

132 As no transamidation was observed, we searched for genes for a

133 Inhibitors of transamidation were effective in blocking the hydrolysis