ライフサイエンスコーパス: transcribed spacer

1 to the rDNA promoter and to the 5'- external transcribed spacer.

2 region spanning the promoter and 5' external transcribed spacer.

3 included the 16S rRNA gene and the internal transcribed spacer.

4 sts and, in phase 2, sequencing the internal transcribed spacers.

5 kinetoplast DNA (kDNA) PCR, nested internal transcribed spacer 1 (ITS-1) PCR, and a PCR-hybridizatio

6 fic probes were directed toward the internal transcribed spacer 1 (ITS-1) region and tested in a mult

7 the hypervariable sequences of the internal transcribed spacer 1 (ITS-1) region of the rRNA gene com

8 hological measurements of males and internal transcribed spacer 1 (ITS-1) sequences of rDNA between t

9 he mouse pre-rRNA transcript in the internal transcribed spacer 1 (ITS1) are affected by depletion of

10 Processing in internal transcribed spacer 1 (ITS1) is a key step that separates

11 5-ends at processing site A2 in the internal transcribed spacer 1 (ITS1) region of the rRNA primary t

12 tion and sequencing of the D1D2 and internal transcribed spacer 1 (ITS1) regions of the nuclear ribos

13 M. intracellulare were confirmed by internal transcribed spacer 1 (ITS1) sequencing and characterized

14 generated by Rat1 digestion of the internal transcribed spacer 1 (ITS1) spacer from cleavage site A(

15 To test this hypothesis, the internal transcribed spacer 1 (ITS1), 5.8S, and ITS2 from eight h

16 equences from the 5'-ETS core, 18S, internal transcribed spacer 1 (ITS1), and 28S segments and also h

17 PCR amplification and sequencing of internal transcribed spacer 1 (ITS1), was employed.

18 as confirmed by sequencing of the internally transcribed spacer 1 (ITS1)-5.8S-ITS2 rRNA-encoding regi

19 as confirmed by sequencing of the internally transcribed spacer 1 (ITS1)-5.8S-ITS2 rRNA-encoding regi

20 cation showed 100% concordance with internal transcribed spacer 1 (ITS1)/ITS2 sequencing and proved t

21 bind overlapping regions within the internal transcribed spacer 1, and both bind directly over cleava

22 RNA processing intermediate, the 5' internal transcribed spacer 1, indicate that bud23 mutants are de

23 We used internal transcribed spacer 1-based metabarcoding to compare feca

24 subunit RNA gene (D1-D2 region) and internal transcribed spacers 1 and 2 (ITS1 and ITS2 regions), hav

25 nd Tibet, and the nuclear ribosomal internal transcribed spacer-1 sequences from each sample were obt

26 Sequences of the Internal Transcribed Spacer 2 (ITS-2) regions of the nuclear rDNA

27 rs to amplify a segment of the rRNA internal transcribed spacer 2 (ITS2) from multiple Leishmania spe

28 ribosomal DNA (rDNA) and 5.8S rDNA/internal transcribed spacer 2 (ITS2) Malassezia-specific PCR prim

29 nventional PCR amplification of the internal transcribed spacer 2 (ITS2) region (ITS2-PCR) followed b

30 DNA sequence analysis of the internal transcribed spacer 2 (ITS2) region of rRNA genes from re

31 on of sequence polymorphisms in the internal transcribed spacer 2 (ITS2) region of the rRNA genes as

32 ific polymorphisms in the noncoding internal transcribed spacer 2 (ITS2) region of the rRNA operon pr

33 found that sequence analysis of the internal transcribed spacer 2 (ITS2) region provided further iden

34 exit, and the domain including the internal transcribed spacer 2 (ITS2) that separates 5.8S and 25S

35 y of these proteins bind at or near internal transcribed spacer 2 (ITS2), but in their absence, ITS1

36 PCR amplicons from the internal transcribed spacer 2 and the D2 region of 28S ribosomal

37 encing analysis of a portion of the internal transcribed spacer 2 region (ITS2) for identification of

38 probes were designed to target the internal transcribed spacer 2 region of Coccidioides.

39 onucleotide probes, directed to the internal transcribed spacer 2 region of ribosomal DNA from Asperg

40 fects in processing of the pre-rRNA internal transcribed spacer 2 region.

41 for the endonucleolytic cleavage in internal transcribed spacer 2 that separates the 5.8S rRNA from t

42 g amplicon sequencing of the fungal internal transcribed spacer 2, we studied the root and rhizospher

43 Independently, the ribosomal DNA internal transcribed spacer-2 (ITS-2) regions from these species

44 The panfungal PCR using internal transcribed spacer 3 (ITS3) and ITS4 primers yielded a p

45 The T.thermophila 5' non-transcribed spacer (5' NTS) is sufficient for replicatio

46 nts that include cleavage of the 5' external transcribed spacer (5'ETS) and 18S rRNA maturation.

47 The 5' external transcribed spacer (5'ETS) is removed from pre-small sub

48 ive sequence analyses by use of the internal transcribed spacer and beta-tubulin regions.

49 ed by DNA sequencing using both the internal transcribed spacer and D1/D2 region of the 28S ribosomal

50 uences and the boundary between the external transcribed spacer and the 18S coding sequence in a clon

51 owed that a large segment of the 5' external transcribed spacer and the entire first internal transcr

52 biogenesis complexes assist the 5' external transcribed spacer and U3 small nucleolar RNA in providi

53 terminator sequences are present in rDNA non-transcribed spacers and a region immediately preceding t

54 , and also sequence analysis of the internal-transcribed-spacer and D1/D2 rDNA regions, the yeast was

55 C-01 nucleotide sequences (gltA and internal transcribed spacer) and protein band banding pattern wer

56 nteractions, designated the U3-ETS (external transcribed spacer) and U3-18S duplexes, are essential t

57 ication initiates from origins in the 5' non-transcribed spacer, and forks moving toward the center o

58 , the major SIR-Responsive Region in the non-transcribed spacer, and SRR2, in the 18S rRNA coding reg

59 Nevertheless, embedded in the internal transcribed spacers are several short sequence elements

60 e identified by sequencing the 5.8S internal transcribed spacer as Pichia fermentans, Wickerhamomyces

61 scribed spacer and the entire first internal transcribed spacer, both of which flank 18S rRNA, are no

62 The transcribed spacers differ very extensively from those o

63 ns and deletions have played a major role in transcribed spacer divergence in Xenopus.

64 h mitochondrial (cox1) and nuclear (internal transcribed spacer) DNA data from the Schistosoma eggs o

65 is in turn implies that large regions of the transcribed spacers do not play a sequence-specific role

66 osomal and transfer RNA maturation, external transcribed spacer (ETS) and internal transcribed spacer

67 n in rRNA or snRNA processing, 5' externally transcribed spacer (ETS) degradation, or viability.

68 did not contain the short-lived 5'-external transcribed spacer (ETS) leader segment upstream from th

69 e interaction with region E1 of the external transcribed spacer (ETS) of pre-rRNA.

70 u hybridization with a probe to the external transcribed spacer (ETS) region of the pre-rRNA shows th

71 ternal transcribed spacer (ITS) and external transcribed spacer (ETS) sequences.

72 ge and complementary regions in the external transcribed spacer (ETS); these interactions are phyloge

73 better target than the 16S-23S rRNA internal transcribed spacer for DNA sequence-based species identi

74 necessary for the removal of the 3' external transcribed spacer from 28S rRNA and productive downstre

75 is required for cleavage of the 3' external transcribed spacer from unprocessed pre-rRNA and for pro

76 d of PAM based on sequencing of the internal transcribed spacers, including the 5.8S rRNA genes.

77 soils were pyrosequenced for fungal internal transcribed spacer (ITS) amplicons.

78 olds were sequence identified using internal transcribed spacer (ITS) and 28S (yeasts) or ITS, transl

79 made using two genetic markers, the internal transcribed spacer (ITS) and a fragment of the beta-tubu

80 creened by sequence analysis of the internal transcribed spacer (ITS) and D1/D2 ribosomal DNA regions

81 ic range of the tribe, with nuclear internal transcribed spacer (ITS) and external transcribed spacer

82 is, and Debaryomyces fabryi using intergenic transcribed spacer (ITS) and/or intergenic spacer (IGS)

83 encing methods with confirmation by internal transcribed spacer (ITS) and/or partial 16S rRNA gene se

84 The internal transcribed spacer (ITS) as one part of nuclear ribosoma

85 us sequence analysis using rpoB and internal transcribed spacer (ITS) as targets was performed.

86 n based on pyrosequencing of fungal internal transcribed spacer (ITS) barcode markers.

87 Taxa in internal transcribed spacer (ITS) Clade 6 are especially numerous

88 bacterial 16S rRNA gene and fungal internal transcribed spacer (ITS) copy numbers and extracellular

89 ermatrix data set was combined with internal transcribed spacer (ITS) data sets for Astraeus, Calosto

90 f ECM fungi was determined using an internal transcribed spacer (ITS) database terminal restriction f

91 g to isolate and characterize nrDNA internal transcribed spacer (ITS) homeologues from multiple acces

92 The sequences of the internal transcribed spacer (ITS) of Pneumocystis carinii f. sp.

93 Variation in the internal transcribed spacer (ITS) of the rRNA (rrn) operon is inc

94 DNA barcoding of nuclear ribosomal internal transcribed spacer (ITS) of the rRNA gene with fungal sp

95 n the number of GTTT repeats in the internal transcribed spacer (ITS) of the rRNA have been described

96 We found that the internal transcribed spacer (ITS) of the X-linked rDNA has two di

97 conserved portion of the T. foetus internal transcribed spacer (ITS) region (ITS1 and ITS2) and the

98 0% in agreement with the contiguous internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) sequenc

99 Sequencing of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) was per

100 h included sequence analysis of the internal transcribed spacer (ITS) region and a fragment of the la

101 Using internal transcribed spacer (ITS) region as the phylogenetic mark

102 f PCR products generated from the intergenic transcribed spacer (ITS) region did not differentiate am

103 R targeting the 16S-23S rRNA gene intergenic transcribed spacer (ITS) region has been proposed as a r

104 gions of the ribosomal cistron, the internal transcribed spacer (ITS) region has the highest probabil

105 The ribosomal DNA internal transcribed spacer (ITS) region of a recently cultured h

106 Primers for the internal transcribed spacer (ITS) region of nuclear rRNA genes an

107 mparing their DNA sequences for the internal transcribed spacer (ITS) region of the 18S-26S ribosomal

108 The internal transcribed spacer (ITS) region of the ribosomal DNA is

109 , a nested PCR method targeting the internal transcribed spacer (ITS) region of the rRNA operon was v

110 control PCR, and PCR targeting the internal transcribed spacer (ITS) region were shown.

111 PCR assay targeting the 16S-to-23S internal transcribed spacer (ITS) region with use of MGB Eclipse

112 The genetic markers used were the internal transcribed spacer (ITS) region, and fragments of the be

113 The contiguous internal transcribed spacer (ITS) region, ITS 1-5.8S-ITS 2, from

114 d on the variable 16S-23S rRNA gene internal transcribed spacer (ITS) region.

115 by 454 pyrosequencing of the fungal internal transcribed spacer (ITS) region.

116 n of the 28S ribosomal gene and the internal transcribed spacer (ITS) regions 1 and 2 of the rRNA ope

117 nce analysis of the ribosomal DNA intergenic transcribed spacer (ITS) regions and the D1-D2 variable

118 The intervening transcribed spacer (ITS) regions between the small- and

119 First, two internal transcribed spacer (ITS) regions flanking the 5.8S subun

120 ty of sequences of the nuclear rDNA internal transcribed spacer (ITS) regions for phylogenetic analys

121 The secondary structure of the internal transcribed spacer (ITS) regions of nuclear rRNA transcr

122 uence analysis of the hypervariable internal transcribed spacer (ITS) regions of ribosomal DNA (rDNA)

123 The nucleotide sequences of internal transcribed spacer (ITS) regions of rRNA genes of 24 iso

124 ysis of the large subunit (LSU) and internal transcribed spacer (ITS) regions of the nuclear ribosoma

125 sequence analysis that included the internal transcribed spacer (ITS) regions of the nuclear ribosoma

126 n was achieved by sequencing of the internal transcribed spacer (ITS) regions of the rRNA gene and by

127 tube nested PCR which amplifies the internal transcribed spacer (ITS) regions of the rRNA genes of hu

128 nce variations in the ITS1 and ITS2 internal transcribed spacer (ITS) regions of the rRNA genes were

129 n of the secondary structure of the internal transcribed spacer (ITS) regions separating nuclear ribo

130 ific primers were designed from the internal transcribed spacer (ITS) regions, ITS1 and ITS2, of the

131 as identified by fungal culture and internal transcribed spacer (ITS) ribosomal DNA (rDNA) sequencing

132 ence analysis of the 283-bp 16S-23S internal transcribed spacer (ITS) sequence showed only 95% identi

133 heir origin was traceable via their internal transcribed spacer (ITS) sequence to five distinct Panic

134 id and nuclear ribosomal DNA (rDNA) internal transcribed spacer (ITS) sequence variation within the C

135 ternal transcribed spacer (ETS) and internal transcribed spacer (ITS) sequences are excised and, as n

136 ylogenetic analysis of nuclear rDNA internal transcribed spacer (ITS) sequences from a worldwide samp

137 cum, Winteraceae, and Zea ribosomal internal transcribed spacer (ITS) sequences.

138 ping was performed using 3'hsp65and internal transcribed spacer (ITS) sequencing.

139 The identification of P. jirovecii internal transcribed spacer (ITS) types was performed on P. jirov

140 sed variation in nuclear sequences (internal transcribed spacer (ITS)) and two types of chloroplast D

141 arkers used for sequencing were the internal transcribed spacer (ITS), a portion of the nuclear large

142 ress this question using the fungal internal transcribed spacer (ITS), which is central in many phylo

143 locations in China were examined by internal transcribed spacer (ITS)-based PCR.

144 Portions of two transcribed spacers (ITS-1 and 5' ETS) and the non-trans

145 ompared with genomic DNA sequences (internal transcribed spacer, ITS).

146 ucted using sequences from nuclear (internal transcribed spacer, ITS; and alcohol dehydrogenase 1A, A

147 it of ribosomal RNA (rRNA), and the internal transcribed spacer ITS1 of rRNA established an order for

148 r distinct sites located within the internal transcribed spacers ITS1 and ITS2 and the 3' external sp

149 The two internal transcribed spacers (ITS1 and ITS2) of nuclear ribosomal

150 zed as a single transcript with two internal transcribed spacers (ITS1 and ITS2), which are removed b

151 e preferentially cleaved the second internal transcribed spacer (ITS2) approximately 250 nt downstrea

152 One [second internal transcribed spacer (ITS2) of the nuclear ribosomal DNA]

153 lear small-subunit rDNA, and second internal transcribed spacer, mitochondrial large-subunit rDNA, an

154 loroplast DNA and nuclear ribosomal internal transcribed spacer (nrITS) phylogenies.

155 ribed spacers (ITS-1 and 5' ETS) and the non-transcribed spacer (NTS) or intergenic spacer (IGS) form

156 Sequence analysis of the ribosomal internal transcribed spacer of 56 Mycobacterium avium complex iso

157 U3 subsequently base pairing to the external transcribed spacer of pre-rRNA, thus positioning U3 snoR

158 gion and complementary bases in the external transcribed spacer of the pre-rRNA.

159 types identified by analysis of the internal transcribed spacer of the rRNA gene.

160 ts containing sequences from the 5' external transcribed spacer or the first internal transcribed spa

161 th polymorphisms of PCR products (intergenic transcribed spacer PCR [ITS-PCR] ribotyping) could disti

162 evidence for recombination in their internal transcribed spacer profiles indicates that they are of r

163 S rRNA gene and fungal nuclear rRNA internal transcribed spacer profiling.

164 The internal transcribed spacer region (ITS) of the nuclear rDNA cist

165 s performed by amplification of the internal transcribed spacer region (ITS) that contained the targe

166 cleotide sequence variations in the internal transcribed spacer region 1 (ITS1) and region 2 (ITS2) o

167 After bronchoscopy, ribosomal internal transcribed spacer region 1 DNA was amplified and sequen

168 PCR amplification and sequencing of internal transcribed spacer region 1.

169 lar screening and DNA sequencing of internal transcribed spacer region 1.

170 g (~80-90%) and members of the same internal transcribed spacer region 2 (ITS2) type were phylogeneti

171 base-pair fragments of DNA from the internal transcribed spacer region 2 from 28 historic herbarium s

172 ncing analysis of 20 nucleotides of internal transcribed spacer region 2 rapidly and robustly disting

173 Amplification of the internal transcribed spacer region and 5.8S rRNA, beta-tubulin, a

174 s of the following three genes: the internal transcribed spacer region and domains D1 plus D2 of the

175 Phylogenetic analysis of the internal transcribed spacer region and portions of the beta-tubul

176 targets including the 16S-23S rDNA internal transcribed spacer region and the rpoB gene (partial seq

177 clade, large-subunit ribosomal and internal transcribed spacer region DNA sequences were determined

178 utility of sequence analysis of the internal transcribed spacer region is highlighted; however, furth

179 ribosomal DNA probe specific to the external transcribed spacer region located at the 5' end of the r

180 at the two tandem termini present in the non-transcribed spacer region located between the sequences

181 phylogeny based on sequences of the internal-transcribed spacer region of nuclear ribosomal DNA to tr

182 irected at the amplification of the internal transcribed spacer region of the Mycobacterium genome wi

183 Internal transcribed spacer region of the nuclear rDNA (ITS) sequ

184 agment length polymorphism of the internally transcribed spacer region of the rRNA operon (ITS PCR-RF

185 ribosomal DNA probe specific to the external transcribed spacer region or to the 28S region of the ri

186 fied polymorphic DNA analysis and internally transcribed spacer region sequencing, by testing species

187 ods were identified on the basis of internal transcribed spacer region sequencing.

188 P. gingivalis-specific amplification was the transcribed spacer region within the ribosomal operon.

189 f the rRNAs (5S, 16S and 23S), an internally transcribed spacer region, and the number of tRNA genes.

190 the bacterial 16S rRNA and fungal internally transcribed spacer region, as well as bacterial genus-sp

191 ndent upon sequence analysis of the internal transcribed spacer region.

192 ently it has mostly been reported in the non-transcribed spacer region.

193 isms (RFLPs) of the PCR-amplified intergenic transcribed spacer regions (including the 5.8S ribosomal

194 A unique primer set amplified internal transcribed spacer regions (ITS) 1 and 2 of the rRNA ope

195 of the nuclear ribosomal RNA (rRNA) internal transcribed spacer regions (ITS1 and -2) to detect and d

196 sm (TRFLP) and sequencing of cloned internal transcribed spacer regions and 16S rRNA genes, respectiv

197 Here we use analyses of the nuclear internal transcribed spacer regions and other genetic traits to r

198 porulating molds (NSM).We sequenced internal transcribed spacer regions from 50 cultures of NSM and f

199 cleotide sequence variations in the internal transcribed spacer regions I and II (ITS1 and ITS2, resp

200 The sequences of ITS1 of the internal transcribed spacer regions of nuclear ribosomal DNA from

201 sing sequences of both ndhF and the internal transcribed spacer regions of nuclear ribosomal DNA reve

202 y PCR with primers specific for the internal transcribed spacer regions of rRNA.

203 Target sequences in the noncoding internal transcribed spacer regions of the rRNA operon were simul

204 tive nucleotide bases in repetitive internal transcribed spacer regions of the rRNA-encoding DNA (rDN

205 the D1/D2 region of ribosomal DNA, internal transcribed spacer regions, and intergenic spacer region

206 e of 18S and 28S ribosomal genes, internally transcribed spacer regions, and mitochondrial genes.

207 riations were identified within the internal transcribed spacer regions.

208 Sequence analysis of the internal transcribed spacer revealed the isolate to be closely re

209 Cloned internal transcribed spacer sequences (ITS-1 and ITS-2, flanking

210 (A0) in the 3' region of vertebrate external transcribed spacer sequences.

211 We performed 18S and internal transcribed spacer sequencing and used the neutral model

212 Bacterial 16S rDNA and fungal internal transcribed spacer sequencing was used to profile organi

213 production is sustained upon introduction of transcribed spacers that reposition SNV RU5 35 to 200 nu

214 nal transcribed spacer or the first internal transcribed spacer, the enzyme preferentially cleaved th

215 the nucleotide sequences of the two internal transcribed spacers, the adjacent ribosomal coding seque

216 fication, zinc finger protein, rRNA external transcribed spacer, thymosin beta-4, cyclin B1 and sever

217 le regions (e.g. 16S rRNA or fungal Internal Transcribed Spacer) to assess diversity or compare popul

218 e of this study was to evaluate the Internal Transcribed Spacer units 1 and 2 (ITS) of the rDNA opero

219 regions of chloroplast DNA and rDNA internal transcribed spacer were incongruent in most New World sp

220 In contrast the non-transcribed spacer, which makes up the majority of the 4