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1 velopment of a gp67-responsive E. coli-based transcription system.
2 tion directly in a highly purified cell-free transcription system.
3 orientation-dependent manner in a cell-free transcription system.
4 ter using a human in vitro RNA polymerase II transcription system.
5 scription in a HeLa nuclear extract in vitro transcription system.
6 n in a reconstituted human RNA polymerase II transcription system.
7 ation of the ctaA promoter using an in vitro transcription system.
8 activity), we utilized the heterologous Gal4 transcription system.
9 3 inhibits rRNA transcription in a cell-free transcription system.
10 us major late promoter in a minimal in vitro transcription system.
11 ng transcript produced in a defined in vitro transcription system.
12 fter transcription using a T7 RNA polymerase transcription system.
13 racts as well as in a reconstituted in vitro transcription system.
14 huronic acid synthesis, by using an in vitro transcription system.
15 ed in a well defined human RNA polymerase II transcription system.
16 s able to mediate activator function in this transcription system.
17 ription process in a highly defined in vitro transcription system.
18 , was analyzed using a synchronized in vitro transcription system.
19 oter in a highly purified HeLa reconstituted transcription system.
20 n, in a yeast-derived TBP-dependent in vitro transcription system.
21 n vitro in a reconstituted RNA polymerase II transcription system.
22 siae Leu3 protein (Leu3p) in a reconstituted transcription system.
23 nterference between PR and TR in a cell-free transcription system.
24 chaeal RNAP in a wholly recombinant in vitro transcription system.
25 in a biochemically defined RNA polymerase II transcription system.
26 n promoters, we used a highly purified human transcription system.
27 emplate using a highly purified human pol II transcription system.
28 transcribe through nucleosomes in a defined transcription system.
29 ivate transcription in a reconstituted human transcription system.
30 ere analyzed using an in vitro reconstituted transcription system.
31 Rgamma function in a reconstituted cell-free transcription system.
32 tors TFIIF and TFIIS in the analogous Pol II transcription system.
33 ntral feature into an otherwise heterologous transcription system.
34 cially activators, regulate a eukaryote-like transcription system.
35 imentary basal initiation apparatus for each transcription system.
36 lementable within the much simpler bacterial transcription system.
37 ells and produce nascent RNAs in an in vitro transcription system.
38 gene activation in a physiologically intact transcription system.
39 ional repression in a reconstituted in vitro transcription system.
40 h can be extended to study other viruses and transcription systems.
41 has come recently from studies in mammalian transcription systems.
42 pendent transcription in mammalian cell-free transcription systems.
43 ases I, II and III than it is to eubacterial transcription systems.
44 Fis in both site-specific recombination and transcription systems.
45 hted a conserved core shared among all three transcription systems.
48 ndent transcription in a homologous in vitro transcription system, accumulates in nuclei of phloem an
49 e elongation we optimized a defined in vitro transcription system and compared results obtained with
50 ctivity occurs in both a typical prokaryotic transcription system and in a eukaryotic-like bacterial
51 transcriptional activity both in an in vitro transcription system and in living cells, which is in co
52 elf were assayed in vitro by using a minimal transcription system and in vivo by assaying beta-galact
53 ed transcription in a well-defined cell-free transcription system and interacted specifically with th
54 assayed as a Gal fusion in the reconstituted transcription system and interacts both with TATA-bindin
55 promoter in a highly purified, reconstituted transcription system and that RA-inducible expression of
56 nduction of the antioxidant response element transcription system and the inhibition of the transcrip
57 activation by liganded TR in the frog oocyte transcription system and was recruited to the T3 respons
59 ons of the chemical master equation for such transcription systems as a function of time, we here dev
61 ucted a red light-sensitive Escherichia coli transcription system based on a chimera between the red/
62 y DNAzymes or ribozymes, this ATP- initiated transcription system based on the T7 phi2.5 promoter can
63 studied nuclear, bacterial, or bacteriophage transcription systems but that similarities are found on
64 -less transcription in a Drosophila in vitro transcription system, but the mechanism responsible for
65 eveloped a thyroid hormone dependent in vivo transcription system by introducing TRs and RXRs (9-cis-
66 ransfected hepatoma cells and in a cell-free transcription system by the binding of factors to this D
67 by human c-Jun in a human RNA polymerase II transcription system composed of highly purified recombi
68 chromatin templates assembled in vitro and a transcription system composed of the human general trans
70 l activation, we established a reconstituted transcription system consisting of human components that
71 imately P in intact cells and in an in vitro transcription system consisting of purified bacterial co
72 ntial terminators was observed in a purified transcription system consisting of template, polymerase,
73 clobutane pyrimidine dimer using an in vitro transcription system consisting of templates containing
74 ds and the phytonutrient regulators of these transcription systems contain electrophilic active group
75 stituted a highly purified RNA polymerase II transcription system containing chromatin templates asse
76 ptor-mediated silencing, we used a cell-free transcription system containing HeLa nuclear extracts in
77 I) was examined in a highly defined in vitro transcription system containing Pol II and purified fact
78 sh DPE-specific transcription in an in vitro transcription system containing TFIID, Mediator, and the
79 transcription, we have developed an in vitro transcription system derived from bovine retinal nuclear
82 these mutants was examined both in cell-free transcription systems derived from hepatoma (HepG2) and
83 purified from wild-type cells in an in vitro transcription system designed to assay active Pol I-Rrn3
85 ified LRPPRC to a recombinant human in vitro transcription system did not activate mtDNA transcriptio
89 transition during the evolution of eukaryal transcription systems, exhibiting a relatively complete
90 ed transactivation, we developed a cell-free transcription system for 1,25(OH)2D3 signaling by utiliz
93 ssay, making this the first general in vitro transcription system for the simultaneous analysis of al
94 g a faithful recombinant human mitochondrial transcription system from Escherichia coli, we demonstra
95 The development of a recombinant in vitro transcription system has allowed for a detailed molecula
96 The development of a reconstituted chromatin transcription system has allowed us to isolate a novel c
98 pret these results to mean that the archaeal transcription system has retained more ancestral charact
99 ort hairpin RNAs via the use of PolIII-based transcription systems has proven to be an effective mech
101 d in the human immunodeficiency virus type 1 transcription system, hnRNP U inhibits elongation rather
102 t the development of a green light-inducible transcription system in E. coli based on a recently disc
104 ence points to a larger role for the Pol III transcription system in various other nuclear processes,
105 anscription with the highly resolved Pol III transcription system in vitro was also diminished when r
107 tant SRC-1 with liganded PR in the chromatin transcription system in vitro, the basic helix-loop-heli
110 conclusion comes from use of an in vitro GAS transcription system in which CovR was sufficient to med
111 is a special cytoplasmic membrane-associated transcription system in which DNA-dependent RNA polymera
112 RNA polymerase II, we developed a chromatin transcription system in which periodic nucleosome arrays
113 These compounds markedly potentiate chimeric transcription systems in cell-based assays and strikingl
114 esults indicate the presence of TATA-unified transcription systems in contemporary eukaryotes and pro
115 ntitermination occurs in vitro in a purified transcription system, in the absence of ribosomes or acc
116 e within the cytoplasm and encode a complete transcription system, including a multisubunit RNA polym
118 btained in a reconstituted RNA polymerase II transcription system, indicated that the promoter escape
119 se) can be reproduced in a purified in vitro transcription system, indicating that the nascent transc
121 Drosophila TFlIB, a highly purified in vitro transcription system is generated that has not previousl
122 Addition of crude cell extract to the simple transcription system leads to repression of all three pr
123 r or linear DNA was incubated in an in vitro transcription system made from a whole-cell extract of H
124 s, we speculate that the human mitochondrial transcription system may have evolved to differentially
125 of SRC-1 and SMRT contained in our chromatin transcription system modulates agonist/antagonist effect
127 l nuclear extract (NE) based in vitro runoff transcription system of core beta-pol promoter human DNA
128 This study provides new insights into the transcription system of euglenoid plastids, the organiza
130 tion in a unidirectional manner in a HeLa mt transcription system, only in the presence of purified m
131 ribe the development of a versatile in vitro transcription system optimized for the expression of MSS
132 or in the reconstituted frog oocyte in vivo transcription system, overexpression of Dot1L enhances g
134 ffects on basal transcription in an in vitro transcription system reconstituted from purified compone
135 ion was studied in a human RNA polymerase II transcription system reconstituted from recombinant and
136 his study, we describe a mammalian cell-free transcription system reconstituted with only recombinant
137 ter escape by RNA polymerase II in a minimal transcription system reconstituted with purified polymer
138 in the absence of an ATP cofactor in a basal transcription system reconstituted with purified RNA pol
139 llenge assays performed in a human cell-free transcription system reconstituted with recombinant gene
140 pends strongly on ATP, TFIIE, and TFIIH in a transcription system reconstituted with RNA polymerase I
142 We first compared Eve activities in in vitro transcription systems reconstituted with either all the
143 phoretic mobility shift assays and cell-free transcription systems reconstituted with purified genera
144 tivation of the bvgR promoter in an in vitro transcription system requires the addition of phosphoryl
146 an essential component of all three nuclear transcription systems, sharply kinks the TATA box at two
149 of the promoter in an in vitro reconstituted transcription system shows that CBF activates transcript
150 quired for NFATp-mediated activation in this transcription system, since TATA-binding protein (TBP) a
151 f recombinant mutants in a reconstituted RSV transcription system suggested that the divalent-cation-
153 e development of a transcription and reverse-transcription system that can replicate unnatural geneti
154 been investigated using a purified in vitro transcription system that does not assemble chromatin.
155 hese findings reveal a specialized TCT-based transcription system that is directed toward the synthes
156 ed with recombinant Drosophila TBP to give a transcription system that is nearly free of contaminatin
157 anscription, we have established an in vitro transcription system that is responsive to large T antig
159 re, we established a MyoD-dependent in vitro transcription system that permits us to determine the ro
162 is formed as a result of the self-organizing transcription system that regulates GLT expression and m
163 n alternations in expression in the in vitro transcription system that were predicted by the mechanis
164 NF-4 functions, we have established in vitro transcription systems that faithfully recapitulate HNF-4
166 We used a rice whole-cell extract in vitro transcription system to characterize the functional inte
167 have used an in vitro chromatin assembly and transcription system to compare the transcriptional acti
168 e have previously used a homologous in vitro transcription system to define functional elements of th
169 ing a highly purified reconstituted in vitro transcription system to demonstrate how pRB can repress
170 ties as DNA templates in a purified in vitro transcription system to demonstrate transcriptional coup
171 argely in exon 1, we established an in vitro transcription system to evaluate activation of transcrip
172 have used an in vitro chromatin assembly and transcription system to examine the biochemistry of inte
173 Additionally, we have developed an in vitro transcription system to generate RNase-resistant RNA tem
176 including an in vitro chromatin assembly and transcription system, to examine the effects of histone
177 including an in vitro chromatin assembly and transcription system, to examine the functional role for
179 transfected cells (TRalpha) and in cell free transcription systems (TRalpha and vitamin D receptor).
180 l promoter system in which a tissue-specific transcription system under the control of a cancer-speci
182 , the template that was used in the in vitro transcription system was a large covalently, closed circ
185 A DNA replication-independent late gene transcription system was established by cotransfecting p
188 A highly reconstituted RNA polymerase II transcription system was refractory to the effect impose
192 lly, using a fully recombinant mitochondrial transcription system, we demonstrate that MRPL12 stimula
193 ing a highly purified in vitro mitochondrial transcription system, we found that 1) the mtRNAP requir
197 By adapting the tetracycline-regulated gene transcription system, we generated a murine model where
199 l nuclear extract (NE)-based in vitro runoff transcription system, we have examined the effect of Sp1
201 ere, using a fully native S. aureus in vitro transcription system, we provide the first molecular and
202 ng a highly purified, reconstituted in vitro transcription system, we show that HSF-4a represses basa
203 the M. jannaschii high-temperature in vitro transcription system, we show that Ptr2 is a potent tran
205 h-resolution and wholly recombinant archaeal transcription systems, we are beginning to understand th
206 een reconstituted S. pombe and S. cerevisiae transcription systems, we confirmed previous observation
209 stablished the existence of a second plastid transcription system which does not utilize E.coli-like
211 transactivation, we established an in vitro transcription system with chromatin templates, in which
212 the initiation properties of a mycobacterial transcription system with E. coli RNAP on two different
213 so be observed in this highly purified human transcription system with either mouse or yeast TBP, TAF
216 nd Mastermind-like 1 (MAML1), in an in vitro transcription system with purified factors and naked DNA
219 (CPD) using enzymatic probes and an in vitro transcription system with purified RNA polymerase II (RN
221 lated transcription using a defined in vitro transcription system with RNA polymerase as the only pro
223 The addition of purified H-NS to an in vitro transcription system yielded a fivefold or greater reduc
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