ライフサイエンスコーパス: transesterification

1 (hydrolysis), followed by hairpin formation (transesterification).

2 which contains the catalytic centre for the transesterification.

3 e or protein-surface hydroxyl groups through transesterification.

4 eceptor is a functional element required for transesterification.

5 ion of the C-26 primary alcohol by catalytic transesterification.

6 n the rate and extent of single-turnover DNA transesterification.

7 n the rate and extent of single-turnover DNA transesterification.

8 n the rate and extent of single-turnover DNA transesterification.

9 ceosome, but were active in promoting step 2 transesterification.

10 tly transferred to an RSS sequence by direct transesterification.

11 utions of phosphodiester connectivity to DNA transesterification.

12 ially impairs nicking, with little effect on transesterification.

13 ng, followed by hairpin formation via direct transesterification.

14 e may be the general base that catalyzes the transesterification.

15 ged for ethyl ester groups by base-catalyzed transesterification.

16 leosides and phosphates to site affinity and transesterification.

17 n the observed rate constants of docking and transesterification.

18 an active site tyrosine directly involved in transesterification.

19 ollowed by iodide substitution and catalytic transesterification.

20 t an intermediate required for initiation of transesterification.

21 - and strand-specific manner by catalysing a transesterification.

22 s attributed to genome cleavage via alkaline transesterification.

23 nique lactone could arise from a postketidal transesterification.

24 l carbon of benzaldehyde, and intramolecular transesterification.

25 tion is substantially disfavored relative to transesterification.

26 tic hydroxyl groups are fully preserved from transesterification.

27 idine units, are effective catalysts of HPNP transesterification.

28 ysis and nucleotide-dependent intramolecular transesterification.

29 ytic core of Rag1 specifically important for transesterification.

30 .1), chain extension experiments and minimal transesterifications.

31 The +3 oxoG modification slowed transesterification 35-fold, whereas other 8-oxo modific

32 ex, with a greater effect on nicking than on transesterification; a conservative glutamine substituti

33 In the second step, Rep transesterification activity cleaves the trs.

34 transferred to cholesterol, but with a lower transesterification activity than mammalian LCAT.

35 se activity (namely specific DNA binding and transesterification activity) have been mapped to the am

36 c carbenes (NHC) are efficient catalysts for transesterification/acylation reactions involving second

37 etyl-ADP-ribose was formed by intramolecular transesterification after enzymatic release into bulk so

38 In situ transesterification allows for rapid access to a variety

39 A high-pressure transesterification allows for the selective ring-enlarg

40 alyze RNA cleavage by hydrolysis rather than transesterification, although normally the hydrolysis re

41 ctrophilic aromatic substitution followed by transesterification and a final dehydration.

42 A modifications at +2A reduced the extent of transesterification and elicited rate decrements of 200-

43 tors that influence the rates of undesirable transesterification and epimerization side reactions at

44 Effective suppression of undesirable transesterification and epimerization was achieved even

45 s of Rag1 that are specifically required for transesterification and suggest an unexpected role for R

46 e strands on the rate of single-turnover DNA transesterification and the cleavage-religation equilibr

47 e strands on the rate of single-turnover DNA transesterification and the cleavage-religation equilibr

48 xation of supercoiled DNA, site-specific DNA transesterification, and DNA strand transfer.

49 The contribution of each base to genome transesterification, and hence inactivation, could be re

50 Friedel-Crafts alkylation, 1,4-AlCl3 shift, transesterification, and protodealumination in a "single

51 ins capable of macrocyclization, hydrolysis, transesterification, and pyrone formation that channel r

52 e, subpopulations bound by MobA that undergo transesterification, and support efficient termination o

53 In addition, stimulation of transesterification appears to require the C-terminal do

54 d tyrosine side chains responsible for TopIB transesterification are conserved and essential in mimiv

55 G substrates and the rate-limiting step for transesterification are observed.

56 base hydrolysis) and by isotope enrichment (transesterification) are demonstrated, their 2-D plots a

57 r, and catalyst solubility while eliminating transesterification as a detrimental side reaction.

58 e of base, the imidazolium species catalyzed transesterifications as well as amidations in a manner s

59 The RNase reaction occurs via transesterification at the scissile ribonucleotide to fo

60 The mechanism entails concerted transesterifications at two recognition sites, 5'-CCCTT

61 The mechanism entails concerted transesterifications at two recognition sites, 5'-CCCTT/

62 degrees C, some stereoselectivity is lost as transesterification becomes significant, and at 60 degre

63 amino acid side chains that are involved in transesterification between DNA and the active site tyro

64 ses introns from pre-mRNAs in two sequential transesterifications-branching and exon ligation-catalys

65 +2 adenosine nucleosides enhance the rate of transesterification by 20- and 1,000-fold respectively a

66 apping +1S BcPh dA adduct slowed the rate of transesterification by a factor of 2700, with little eff

67 er (between the +1 and -1 base pairs) slowed transesterification by a factor of 450.

68 wnward arrowN(2')p(5')N) reduced the rate of transesterification by a factor of 500.

69 k at the +2 phosphate also slows the rate of transesterification by approximately 500-fold.

70 strand (5'-CCCTTp / NpN) enhance the rate of transesterification by factors of 40 and 25, respectivel

71 carries out multiple turnovers and acts as a transesterification catalyst with k(1)/k(uncat) of 2.2 x

72 Transesterification catalysts based on stereochemically

73 ydride thermoset networks in the presence of transesterification catalysts.

74 at 28 degrees C via direct esterification or transesterification catalyzed by the versatile lipase/st

75 te that Topo-(81-314) is fully competent for transesterification chemistry, but is compromised with r

76 s of specific amino acids to DNA binding and transesterification chemistry, we introduced alanine sub

77 were defective for ATP hydrolysis and step 2 transesterification chemistry.

78 catalytic domain, Topo(81-314), suffices for transesterification chemistry.

79 ow that Arg-130 and Lys-167 are required for transesterification chemistry.

80 The title compound 1 was then obtained by transesterification, desilylation, and hydrochloride sal

81 transformations, based on the intramolecular transesterification, E1cB tandem eliminations, 1,2-addit

82 carbonyl carbon, and (4) an SIR2-independent transesterification equilibrating the 2'- and 3'-AADPRs.

83 ls and antioxidants were formed by enzymatic transesterification, exploring canola oil and naturally

84 dicate that the reaction involves an initial transesterification, followed by an intramolecular rearr

85 olves Lewis acid catalyzed or DMAP catalyzed transesterification, followed by intramolecular conjugat

86 for catalysis of RNA cleavage by 2'-hydroxyl transesterification, forming 2',3'-cyclic phosphate and

87 own or engineered inteins corresponds to the transesterification from an oxoester to a thioester, whi

88 ansient, which is apparently associated with transesterification, has an observed rate constant that

89 ut no other residues in Rag1 responsible for transesterification have been identified.

90 e with industrial significance for catalyzed transesterification, hydrolysis, and esterification reac

91 onic acid monomethyl esters in high yield by transesterification in acidulated methanol.

92 drolysis reaction occurs in vivo in place of transesterification in the mitochondria of yeast strains

93 NAzyme is a DNA metalloenzyme catalyzing RNA transesterification in the presence of divalent metal io

94 Here we present a sensitive assay for DNA transesterification in which catalysis by human immunode

95 erves as the general acid during the initial transesterification, in agreement with hypotheses based

96 lide and provided evidence that the proposed transesterification indeed provides a survival advantage

97 rbenes (NHC), are efficient catalysts in the transesterification involving numerous esters and alcoho

98 he catalyst towards monomer propagation over transesterification is attributed to a selective activat

99 The first relaxase-catalysed transesterification is essential for initiation of conju

100 The relaxase-catalysed transesterification is isoenergetic and reversible; a se

101 BPdG inhibition of transesterification is likely caused by steric exclusion

102 When pdG is substituted for prG, no transesterification is observed, and fluorescence quench

103 iboronic ester small molecules with variable transesterification kinetics to dynamically cross-link 1

104 via orthometalation of P-OAr groups and then transesterification liberates the product phenol.

105 ion is isoenergetic and reversible; a second transesterification ligates the nicked DNA.

106 More generally, transesterification may represent a previously undescrib

107 are consistent with only the intramolecular transesterification mechanism.

108 eps in Hh autoprocessing, N-S acyl shift and transesterification, must be coupled for efficient Hh ch

109 In vitro, CUS1 catalyzes the self-transesterification of 2-monoacylglycerol of 9(10),16-di

110 n this C-O bond forming reaction, formed via transesterification of AcOAr with [Cu(II)]-O(t)Bu interm

111 ases and tyrosine recombinases cleave DNA by transesterification of an active site tyrosine to genera

112 ctonization method involving an NaH promoted transesterification of an advanced intermediate bearing

113 icient, highly regioselective hydrolysis and transesterification of dimethyl 3-benzamidophthalates in

114 lated gene 6 protein (TSG-6), which supports transesterification of heavy chains to hyaluronan.

115 mato (Solanum lycopersicum) fruit occurs via transesterification of hydroxyacylglycerol precursors, c

116 Free lipase (Candida rugosa) catalyzed the transesterification of methyl methacrylate in 1-butyl-3-

117 The initial rate of lipase-catalyzed transesterification of methyl methacrylate in these ioni

118 The product of transesterification of phospholipid acyl chains and unes

119 fatty acid methyl esters (FAMEs) produced by transesterification of plant oils with methanol.

120 The production of biodiesel from the transesterification of plant-derived triglycerides with

121 eactions were examined: (i) enantioselective transesterification of racemic proxyphylline with vinyl

122 lytic RNA that catalyses the endonucleolytic transesterification of RNA in a highly sequence-specific

123 ytic RNA which catalyses the endonucleolytic transesterification of RNA in a highly sequence-specific

124 calix[4]arene spacer was investigated in the transesterification of RNA models HPNP and four diribonu

125 idation of dimethyl E-oct-4-enedioate and by transesterification of the epoxide derived from the gluc

126 to the surface, the peptides accelerate the transesterification of the p-nitrophenyl ester of N-carb

127 as confirmed through the holo ACP-dependent transesterification of the released product.

128 Kinetics of transesterification of the RNA model substrate 2-hydroxy

129 es sequence-specific cleavage of RNA through transesterification of the scissile phosphate.

130 he Sln9 thioesterase domain established that transesterification of the serine residue of desmethylsa

131 aesters (26) were prepared by base-catalyzed transesterification of the tetraethyl ester (25).

132 The transesterification of total lipid extracts furnished FA

133 His-265 in vaccinia topoisomerase) catalyzes transesterification of tyrosine to the scissile phosphod

134 st contains two triphenylsiloxy ligands, the transesterification of vanadate occurs via sigma-bond me

135 ssist efficiently the general base-catalyzed transesterification often occurring in active sites of n

136 Models for editing propose transesterification or endonuclease plus RNA ligase reac

137 nal selectivity for copolymerization without transesterification or epimerization side reactions.

138 NA-hydrolyzing deoxyribozyme leads either to transesterification or hydrolysis, depending on exclusio

139 UTP by two successive cleavage-ligations or transesterifications, or from the 3' end of the gRNA by

140 ly involved in catalysis of both nicking and transesterification, our observations indicate that thes

141 5 in the nonscissile strand had no effect on transesterification per se but had synergistic effects w

142 by a combination of anionic and ring-opening transesterification polymerizations.

143 ken to determine possible mechanisms for the transesterification processes that are consistent with e

144 ormance and oxidative stability of the final transesterification products were evaluated.

145 The NHC-catalyzed transesterification protocol was simplified by generatin

146 element contributes minimally to the rate of transesterification provided that the substrate is other

147 and S BPdA modifications at +1A reduced the transesterification rate by a factor of 700-1000 without

148 bstitutions for +4G and +3G had no effect on transesterification rate, implying that the guanine exoc

149 e strand (3'-GGGpApApTpApA) had no effect on transesterification rate.

150 of the C-ring using a vinylogous Knoevenagel/transesterification reaction and construction of the D-r

151 It promotes the second transesterification reaction and then catalyzes the ATP-

152 OH of the bulged adenine participates in the transesterification reaction at the 5'-exon and forms th

153 er is responsible for two hydrolysis and one transesterification reaction at the same transposon end.

154 f the linear segment to the PEG network by a transesterification reaction between the hydroxyl groups

155 ementioned photocyclization, the rate of the transesterification reaction between vinyl acetate and a

156 B-091, which involves a novel intermolecular transesterification reaction catalyzed by a type I thioe

157 e of a properly positioned 5'-OH terminus in transesterification reaction chemistry, but they also ra

158 stricting spliceosomal splicing to the first transesterification reaction differs substantially among

159 a 2',3'-cyclic phosphate, Pb(2+) catalyzes a transesterification reaction followed by hydrolysis of t

160 n-canonical 5' splice site blocks the second transesterification reaction in Aspergillus species.

161 se that activates spliceosomes for the first transesterification reaction in pre-mRNA splicing.

162 al for female fertility and accompanied by a transesterification reaction in which the heavy chains (

163 t activates the spliceosome before the first transesterification reaction of pre-mRNA splicing.

164 hate, prG, substrate on various steps in the transesterification reaction of prG with 5' pyrene-label

165 Mrs1 is known to function in the initial transesterification reaction of splicing.

166 at the healing kinetics is controlled by the transesterification reaction rate.

167 The reaction proceeds by a two-step transesterification reaction requiring the formation of

168 osa cells within the follicle may catalyze a transesterification reaction resulting in an exchange of

169 ic cleavage of a phosphodiester linkage by a transesterification reaction that entails the attack of

170 cking one DNA strand, followed by a one-step transesterification reaction that forms a DNA hairpin st

171 thiophilic ion such as Mn2+ allows the first transesterification reaction to occur in the U6/sU80(Sp)

172 zyme loading, shaking speed and time) on the transesterification reaction was investigated to give op

173 While Mg(2+) and Zn(2+) catalyze only a transesterification reaction with formation of a product

174 The transesterification reaction, and in particular the meth

175 These changes block the first transesterification reaction, as in a subset of mammalia

176 da 3 gene (nda 3-Int3) also blocks the first transesterification reaction, suggesting that early reco

177 identify amino acids that participate in the transesterification reaction, we introduced alanine subs

178 ch point are not required prior to the first transesterification reaction, whereas in mammals the pol

179 de influences the rate of the intramolecular transesterification reaction, with guanosine being more

180 itical role of this 2'-hydroxyl group in the transesterification reaction.

181 avorable K(M) for pG in a ribozyme-catalyzed transesterification reaction.

182 he C75 pK(a) in an intermediate state of the transesterification reaction.

183 ted more optimally downstream is used in the transesterification reaction.

184 trand, that rejoins the ends by a reversible transesterification reaction.

185 structure, prior to the ATP-independent Rep transesterification reaction.

186 ion from precursor RNAs by way of a two-step transesterification reaction.

187 tion of the precatalytic spliceosome for the transesterification reaction.

188 ovided a detailed view of every stage of the transesterification reaction.

189 as the most efficient lipase to catalyze the transesterification reaction.

190 l ADP ribose intermediate and intramolecular transesterification reactions (2' --> 3').

191 rse splices into a DNA site via 2 sequential transesterification reactions and is reverse transcribed

192 at of PAT occurs on the periplasmic face via transesterification reactions between DAT substrates cat

193 plicing of pre-mRNAs involves two sequential transesterification reactions commonly referred to as th

194 NA precursors through two sequential phospho-transesterification reactions in a dynamic RNA-protein c

195 e an environment by which MsAcT can catalyze transesterification reactions in an aqueous medium and s

196 assay to show that HIV-1 IN could carry out transesterification reactions involving DNA 5' hydroxyl

197 secreted mycobacterial proteins and catalyze transesterification reactions that synthesize mycolated

198 nal sequences through sequential nicking and transesterification reactions to yield blunt signal ends

199 e-mRNA) splicing proceeds by two consecutive transesterification reactions via a lariat-intron interm

200 ovalent self-assembly through energy-neutral transesterification reactions, a process called autoreco

201 eosome in two sequential but tightly coupled transesterification reactions, TER1 only undergoes the f

202 ger RNA precursors by the spliceosome in two transesterification reactions-branching and exon ligatio

203 cking and polar edge interactions to the DNA transesterification reactions.

204 ide Baeyer-Villiger conditions and selective transesterification reactions.

205 be used for the catalysis of small molecule transesterification reactions.

206 e reactivity versus a 5-ethyl ester in basic transesterification reactions.

207 pliceosomal introns splice by two sequential transesterification reactions.

208 g cyanosilylation, benzoin condensation, and transesterification reactions.

209 h conjugation: the site- and strand-specific transesterification (relaxase) reaction that provides th

210 Here we discuss catalytic esterification and transesterification solutions to the clean synthesis of

211 ng as follows: it is required for the second transesterification step and for the release of mature m

212 a rearrangement that accompanies the second transesterification step deposits Prp22 on the mRNA down

213 Evidence for the transesterification step is provided by detection of a 3

214 AH protein Prp22 is important for the second transesterification step of pre-mRNA splicing, and it is

215 e branch site, the nucleophile for the first transesterification step of splicing, is nearly invarian

216 ions as the 3' splice site during the second transesterification step of splicing.

217 The mechanism of the backbone cleavage-transesterification step of the RNase A enzyme remains c

218 nucleophilic 3' ends used in the postulated transesterification step.

219 Pre-mRNA splicing involves two transesterification steps catalyzed by the spliceosome.

220 ects both the N-S acyl rearrangement and the transesterification steps in the splicing pathway.

221 , acting as a rogue nucleophile, can disrupt transesterification steps of important phosphoryl transf

222 ResT mediates the transesterification steps of resolution using a constell

223 ecific recombinase Cre are essential for the transesterification steps of strand cleavage and joining

224 Both arginines are essential for the transesterification steps of strand cleavage and strand

225 rentially affect the forward and reverse DNA transesterification steps of the vaccinia topoisomerase.

226 active site, which catalyzes the nicking and transesterification steps of V(D)J recombination by a si

227 nd Asn-228 in alpha6) to the DNA binding and transesterification steps.

228 -rRNA proceeds in two consecutive phosphoryl transesterification steps.

229 and-specific break in the DNA backbone via a transesterification that leaves the initiator protein co

230 and activating the downstream nucleophile in transesterification, the second step of protein splicing

231 hange (movement of >9 A) must occur to allow transesterification to be completed.

232 he specificity of vaccinia topoisomerase for transesterification to DNA at the sequence 5'-CCCTT and

233 rmal or near-normal rates of single-turnover transesterification to DNA.

234 nt of the nicking reaction, which involves a transesterification to form a phosphotyrosine bond withi

235 one end of the deleted element, followed by transesterification to generate the macronuclear junctio

236 ase catalyzes DNA cleavage and rejoining via transesterification to pentapyrimidine recognition site

237 mic lid until it interacts with HDL to allow transesterification to proceed.

238 te is ADP-ribosylated followed by a presumed transesterification to release the RNA and generate Appr

239 be the use of tunable rates of boronic ester transesterification to tune the malleability and self-he

240 e tyrosine is responsible for initiating two transesterifications to cleave and then religate the DNA

241 The transesterification transfer of HCs from chondroitin sul

242 ns represent reverse reaction models for RNA transesterification under alkaline conditions.

243 at catalyze the cleavage of the P-O bond via transesterification using the internal hydroxyl group of

244 Moreover, through Lipozyme TL IM-mediated transesterification, valuable 2-phenethyl alcohol-derive

245 Moreover, through Lipozyme TL IM-mediated transesterification, valuable methionol-derived esters w

246 On the other hand, solvent-free transesterification was an extremely efficient mechanism

247 Transesterification was unaffected by BcPh intercalation

248 This reaction involves reversible transesterification where the active site tyrosine of th

249 se pairs by the +2R BcPh dA adduct abolished transesterification, whereas the overlapping +1S BcPh dA

250 rsor autoprocessing and is indispensable for transesterification with cholesterol.

251 ection of alkylpinacolyl boronate esters via transesterification with diethanolamine followed by hydr

252 ned from 2'-acetoxycocaine (12) by selective transesterification with MeOH saturated with dry HCl gas