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1 type (WT), but not AI-forms of FAM20A was co-transfected.
3 ptides directly from the surface of live HIV-transfected 293T cells, HIV-infected B cells, and primar
5 produced the triploid vector AAV2/8/9 by co-transfecting AAV2, AAV8 and AAV9 helper plasmids at a ra
6 lso differences in IT-mediated killing among transfected and infected cell lines that were unrelated
7 by coimmunoprecipitation experiments on both transfected and infected cells and biochemical character
11 to conventional plasmids which usually yield transfected astrocytes only), offering advantages for ta
12 urrent in vivo mRNA delivery systems fail to transfect B lymphocytes and instead primarily target hep
13 an encapsulate mRNA, navigate to the spleen, transfect B lymphocytes, and induce more than 60 pg of p
14 roscopy revealed that nanoplexes efficiently transfect bacterial cells, consistently with biological
18 -regulated genes, we isolated mRNAs bound to transfected biotinylated miR-200c in mouse breast cancer
20 ansfection and persisted for 3 days in cells transfected by pEF-GFP and pUB-GFP plasmids and for at l
25 able, mutation effects were determined using transfected cell lines and peripheral blood from carrier
29 ound to be internalized in both infected and transfected cells after the addition of either RSV-speci
30 L16 are mutually responsive to each other in transfected cells and form complexes that are stable eno
32 h parallel in vitro experiments in mammalian transfected cells and in situ and in vivo experiments in
33 ts ability to form higher-order complexes in transfected cells and in vitro Combining inactive trunca
35 e viral DNA replication centers in both OriR-transfected cells and virus-infected cells, highlighting
36 16, and -18 origin-containing plasmid DNA in transfected cells at concentrations well below its cytot
37 , CCR5, and GPR15 mediated robust entry into transfected cells by pseudotypes carrying SIVagmSab92018
39 Immunoprecipitation experiments performed in transfected cells confirmed absent STAT1 sumoylation for
41 by using in silico prediction and plasma and transfected cells from both patients and control subject
42 l and super-resolution imaging of native and transfected cells include p65 signal transduction activa
43 he AHTM found HPNE, H-ras, Myr-AKT, and Bcl2 transfected cells more compliant relative to controls, c
44 pression, suggesting immune clearance of the transfected cells occurs in immunocompetent animals.
45 ein activation when using membranes from MOR-transfected cells or WT mice but not from MOR KO mice.
48 ation of IFT43 in the retina and transiently transfected cells was examined by RT-PCR, western blot a
49 tive or synergistic promoter activation when transfected cells were treated with combined stimuli.
50 bating membranes prepared from chicken RPE65-transfected cells with liposomes containing 0-1 muM atRE
53 stically, CaMK2D was up-regulated in CEACAM1-transfected cells, effecting phosphorylation of HDAC4 an
56 s of mouse astrotactin-2 (Astn2) produced in transfected cells, we determined that the amino and carb
57 Using biophysical techniques in mammalian transfected cells, we found evidence for selective heter
58 mary and THP1-derived macrophages as well as transfected cells, we further identified the scavenger r
59 deliver cytotoxic agents to infected or Env-transfected cells, we noted disparities in their functio
60 lpha2delta-3 and decreases CaV2.2 current in transfected cells, whereas Neurexin-1alpha has no effect
61 ns interacted with beta-catenin and HMGA1 in transfected cells, which resulted in the nuclear localiz
62 were found to inhibit nuclear import of C in transfected cells, with one able to reduce VEEV replicat
84 donor DNA conjugate is three times better at transfecting cells and inducing HDR, with cationic polym
92 interventions are often based on plasmids or transfected circuits which implement some form of "on-of
94 alone and CREB plus HIPK2 over empty vector-transfected control displayed 14- and 32-fold upregulati
95 G93A transgenic mice and in ALS mutant SOD1 transfected cortical neurons, but the underlying mechani
96 nd p.Ala210Val SLC45A1 variants, measured in transfected COS-7 cells, was approximately 50% (p = 0.01
98 urther, human T-cells co-cultured with miRNA transfected dendritic cells secrete low levels of T help
101 ith p85, independent of adaptor proteins and transfected FLAG-p85 provided evidence that SHP2 binding
102 uciferase-based membrane fusion assay, using transfected fusion and hemagglutinin expression plasmids
103 -fluorescent-protein (GFP)-P. gingivalis-NDK transfected GECs revealed a perinuclear/cytoplasmic loca
104 ciferase reporter expression was measured in transfected H2.35 cells expressing an estrogen receptor
106 o investigate the underlying mechanisms, HBc-transfected HCC cells were characterized by multi-omics
110 ys and patch clamp recordings of transiently transfected HEK-293 cells for electrophysiological chara
112 reflection fluorescence live cell imaging of transfected HEK293 cells, we demonstrated that cAMP sign
116 inocyte cell line (HaCaT) and in transiently transfected HEK293T cells and found that the sensitizing
117 ed in peripheral blood mononuclear cells and transfected HEK293T cells through immunoblotting, immuno
119 nogold labeling, we examined the surfaces of transfected HEp-2 cells and further dissected the proces
122 es, we mapped human PRDM9 binding sites in a transfected human cell line and measured PRDM9-induced h
123 n microscopy studies of ruptured nuclei from transfected human cell lines demonstrate the presence of
125 h TLR2, as determined using a variety of TLR-transfected human embryonic 293 cell lines, while the PF
126 receptor-positive microvesicles derived from transfected human embryonic kidney cells induced a signi
127 nts on fMLP-stimulated neutrophils and 5-LOX-transfected human embryonic kidney cells, propofol atten
128 Although all antibodies detected Siglec-E on transfected human HEK-293T cells, only two reacted with
129 roduction of an array of cytokines in stably transfected human myelomonocytic U937 cells in response
131 adiated, Hey2 siRNA- and Hey2 vector plasmid-transfected human umbilical vein endothelial cells (HUVE
134 urons, astrocytes and oligodendrocytes) were transfected (in contrast to conventional plasmids which
136 and human recombinant kappa opioid receptors transfected into a host cell, two selectively-acting com
137 inally, the p.Glu401Asp mutated DES gene was transfected into cell lines and analyzed by confocal mic
138 itself, failed to generate any current when transfected into Chinese hamster ovary (CHO) cells but,
141 os of rat alpha4 and beta2 subunit cDNA were transfected into human embryonic kidney 293 cells to cre
142 as well as HLA-E*01:01 and HLA-G*01:01 were transfected into lymphoblastoid C1R cells expressing low
144 variants resist conversion to PrP(res) when transfected into scrapie-infected N2a neuroblastoma cell
147 ructs encoding Kv4.3, KChIP1, and DPP10 were transfected into the injured DRGs (defined as DRGs with
153 rget genes at the mRNA and protein levels by transfecting live parasites with ssRNA-hAgo2 complexes.
157 ng domain, is highly up-regulated in CEACAM1-transfected MCF7 cells, and when down-regulated with RNA
158 ere harvested from the mammary fat pad after transfecting MDA-MB-231 breast cancer cells, while BMD c
164 sed by using in silico analysis and in vitro transfecting miRNA mimics in human bronchial epithelial
168 urons, thereby mimicking conditions of shRNA-transfected neurons previously used to characterize GODZ
170 at dividing human and canine cells partition transfected plasmid DNA asymmetrically, preferentially i
171 n the surface of the sporozoite, transiently transfected populations of E. tenella expressing the flu
172 Second, a single variant from a library of transfected, promoter-less plasmids is recombined into t
173 wild type and mutant Cx50 examined by stably transfecting recombinant systems revealed similar protei
176 HK2, and pLSLP-HK3 were constructed and then transfected separately or co-transfected into the cells.
177 hypothesis, Dok-7 expression was reduced by transfecting shRNA-coding plasmids into the tibialis ant
180 es the use of peptide-based nanoparticles to transfect specific microRNA inhibitors or activators int
181 erformance, the biosensor was constructed by transfecting specific hybridoma cells with aequorin repo
183 TET1, 2 and 3) was measured in stable TWIST1 transfected TC28 cells, and TET1 expression was up-regul
184 malian expression vector pCI-neo was used to transfect testes of adjudin-treated ratsversusempty vect
187 mproved antitumor activities into T-cells by transfecting them with mRNAs that encode a key transcrip
188 h support entry and replication of HCV, were transfected these cells with small interfering RNAs targ
190 -CAR and SKOV3-CAR cells (CHO or SKOV3 cells transfected to stably express human coxsackievirus and a
194 ppressed by Mb-siRNA transfection (Mb vector transfected vs. Mb vector, control siRNA transfected vs.
195 tor transfected vs. Mb vector, control siRNA transfected vs. Mb vector, Mb siRNA transfected: 0.15 vs
196 uorescence intensity and percentage of cells transfected was 2-4-fold higher with minicircle DNA.
197 haea considered intractable and difficult-to-transfect, while facilitating fundamental genetic studie
199 ty test based on embryonic cells (C3H10T1/2) transfected with a BMP-responsive element, we sought to
200 ive promoter activity was reduced in neurons transfected with a dominant-negative cAMP response eleme
202 t on the activity of luciferase in the cells transfected with a mutant-TREM1 plasmid carrying mutatio
203 tly reduced the luciferase activity in cells transfected with a plasmid containing FXI-3'UTR.These re
210 s (calpain inhibitor III and calpastatin) or transfected with calpain-1 siRNA demonstrated attenuatio
211 nd-gated ion channels, we employed HEK cells transfected with cDNAs encoding three requisite receptor
212 cell surface were increased in HEK293T cells transfected with CLCA1 constructs containing the VWA dom
213 reover, PTH treatment of HEK293 cells stably transfected with CLDN14-GFP, together with PTH1R, induce
214 by experimental data obtained in HeLa cells transfected with connexin45, which is among connexin iso
216 lony-stimulating factor, compared with cells transfected with control CSF2RB, indicating a dominant-n
217 ore resistant to HSP90 inhibitors than cells transfected with control vectors; inactivation of MIR21
218 thermore, a focused gene array between cells transfected with CREB alone and CREB plus HIPK2 over emp
219 mporal variation in EGFP expression in cells transfected with CRISPR-Cas9 components for gene editing
222 hanisms underlying these effects, cells were transfected with different variants of HIV LTR promoters
223 tures were transformed by ras oncogenes when transfected with DNA from neoplastic cells, but they fai
225 his study, from supernatant of culture cells transfected with DNAs of an Autographa californica multi
227 e for EpoR stabilization, HEK293T cells were transfected with EpoR and/or RNF41 gene expression vecto
229 y transfer measurements in cells transiently transfected with fluorescently labeled SERCA2a revealed
231 ortantly, synaptic NMDAR currents in neurons transfected with GluN2B S1413L in GluN2A/B-deficient mou
232 n, and DNA replication in HepG2 cells stably transfected with HBV DNA, while dimethyl sulfoxide could
234 talized keratinocytes (NIKS) and NIKS stably transfected with HPV16 episomal genomes (NIKS16) were co
235 s as granulosa cells and HEK293 cells stably transfected with human FSHR cDNA expressed FSHR signal.
237 HeLa PKR knock-down cells, which were stably transfected with human or rabbit PKR, revealed that only
238 m lumena in a three-dimensional culture, are transfected with ID4, lumen formation is restored, demon
241 Importantly, we show that leukaemia cells transfected with light-inducible NPs containing RA can e
242 ithelial cells (Caco-2 and IEC-6 lines) were transfected with LNA-anti-uc.173 or uc.173 transgene.
244 HD, including mouse and fly models, neurons transfected with mHTT, HD iPSC-derived neurons, and huma
245 an enamel protein intake assay, enamel cells transfected with miR-153 show a decreased ability to end
250 f hSOD1 aggregates in the cytoplasm of cells transfected with mutants but not in cells transfected wi
251 y increased NaCl-elicited responses in cells transfected with NHE1 siRNA but not in those transfected
253 Moreover, the STAT3 signaling in the cells transfected with nsp5 plasmid was significantly inhibite
255 pcDNA3.1-myc-His-Phe(99)-CaM, but not in ECs transfected with pcDNA3.1-myc-His-Phe(138)-CaM, the lyso
260 relative activity of luciferase in the cells transfected with plasmid contenting wild type-promoter o
261 od mononuclear cells from immune donors were transfected with plasmids coding for the most abundant p
262 expression during HSV infection, cells were transfected with plasmids harboring an unspliced renilla
266 lpha] and interleukin-10 [IL-10]), BMMs were transfected with selected miRNA mimics and inhibitors.
267 micin enhanced readthrough activity in cells transfected with SERPINB7 cDNA carrying the mutation and
268 s is impaired in 3T3-L1 preadipocytes stably transfected with Siah2 shRNA and that overexpression of
271 to AR were significantly decreased in cells transfected with siRNAs against epithelial sodium channe
272 gene expression was reduced in chondrocytes transfected with SIRT1 siRNA or treated with nicotinamid
275 were isolated from heterozygous patients and transfected with SNP-targeted siRNA, using glucan partic
276 e injected with Daun02 in the LHb previously transfected with ss-galactosidase under control of the F
279 in RAST-based assays and with rat basophils transfected with the human FcepsilonRI, respectively.
281 Golden Retriever muscular dystrophy dog were transfected with the large-size PB transposon resulting
283 d in 1alpha,25(OH)2D3-treated HPK1ARas cells transfected with the non-phosphorylatable hRXRalpha Ala-
284 eased luciferase activity in cells that were transfected with the pERRE3tk-LUC plasmid, which demonst
287 ination with confocal microscopy and T cells transfected with the T-cell receptor specific for the bi
288 rocytes were isolated from the intestine and transfected with the uc.173 transgene to increase uc.173
289 Trunk and Torso-like were active when co-transfected with Torso and when presented to Torso-expre
290 ombinant Mos1 or Mboumar-9 transposase is co-transfected with transposon-containing plasmid DNA, it p
293 al epithelial cells and PDAC cell lines were transfected with vectors to overexpress or knock down TF
295 eased dendritic branching, compared to those transfected with wild-type (WT) Crmp4, indicating that t
296 ls transfected with mutants but not in cells transfected with wild-type hSOD1 or those containing a s
299 complex was confirmed in 2780CP/Cl-24 cells transfected with wt p53 or multimer-inhibiting p53(L344P
300 Supporting this notion, HEK293 cells stably transfected with YFP-tagged R753Q TLR2 displayed reduced
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