ライフサイエンスコーパス: transfection

1 tion agent is likely to activate TLR2 during transfection.

2 as transient and lasted for up to 24 h after transfection.

3 epolymerization and apoptosis, and cell line transfection.

4 ithin the first 2 days of a reverse genetics transfection.

5 eprogramming appears to be a hallmark of HBc transfection.

6 enesis that were corrected with NDUFAF6 cDNA transfection.

7 tabolic degradation, thus leading to optimal transfection.

8 nto cell nuclei, which is necessary for gene transfection.

9 derived fibroblasts without repetitive daily transfection.

10 nduced cell differentiation compared with co-transfection.

11 sults in DNA degradation and subsequent poor transfection.

12 (BACs), and then virus is regenerated by DNA transfection.

13 ier than IE2 after CMV infection or MIE gene transfection.

14 ed down by siRNA or overexpressed by plasmid transfection.

15 a single barrier on the route for efficient transfection.

16 caveats, including a heavy reliance on viral transfection.

17 gical disruption and variability inherent to transfection.

18 -9 nuclease (Cas9), a method called acoustic-transfection.

19 ving to other compartments within 30min post-transfection.

20 DNA vaccine carriers for dendritic cell (DC) transfection.

21 but they failed to do so in 80 to 90% of the transfections.

22 ammalian cells using Norwalk virus stool RNA transfection, a reverse genetics system, IFN neutralizat

23 yl phosphorothioate oligonucleotide in vitro transfection abilities.

24 se necessary for efficient antibiotic or DNA transfection activity of LAH4 derivatives.

25 le particle size threshold model to describe transfection activity, we explain how dimensions of amyl

26 PgP/pDNA polyplexes maintain bioactivity for transfection after lyophilization/reconstitution and dur

27 saturated LPA currently used or proposed as transfection agent is likely to activate TLR2 during tra

28 feature is in great contrast to a commercial transfection agent with similar transfection performance

29 need to test the inflammatory properties of transfection agents and proposes the use of docking anal

30 eling with Ferumoxytol, without the need for transfection agents and/or electroporation, allowing cel

31 acids are often complexed with polycationic transfection agents before delivery.

32 peptide derived from the LAH4 family of DNA transfection agents.

33 TAC polymers are biocompatible and efficient transfection agents.

34 Transfection analyses demonstrated that glucagon plus in

35 Transfection analysis in LS180 cells demonstrated that P

36 to a standard EM sample preparation are cell transfection and a 2- to 45-min staining period with 3,3

37 ach that enables highly efficient, localized transfection and allows for transfection of three-dimens

38 ting to a 2.5~3 fold increase on plasmid DNA transfection and an additional 10-55% transgene knockdow

39 ti-GFP IgA antibodies, demonstrating in vivo transfection and expression.

40 based methods for both single and double DNA transfection and for different cell types including adhe

41 red rat sensory neurons following AKAP siRNA transfection and from AKAP-knock-out mice had less PKA a

42 Reporter plasmid transfection and genomic run-on sequence analyses confir

43 t of segment 7 RNPs reconstituted by plasmid transfection and in mutant virus-infected cells.

44 tform, which allows the in vivo preferential transfection and in vivo tracking of TEMCs with the desi

45 onse elements and a combination of transient transfection and knock down assays revealed an important

46 reporter gene was detected a few hours after transfection and persisted for 3 days in cells transfect

47 each mutant in mammalian cells by transient transfection and the identification of thermostable muta

48 ens larvae, after TnBVank1 in vivo transient transfection, and in haemocytes of parasitised larvae.

49 as DiI-SiR are non-toxic and do not require transfection, and their apparent photostability signific

50 The transfection apparatus consists of an ultrasonic transdu

51 n RNA (shRNA)-mediated silencing and miR-155 transfection approaches, we found that CD69 deficiency i

52 ist stimulation of [Ca(2+)]i with beta2AR co-transfection, approximately 53% decrease in [Ca(2+)]i si

53 identified mechanisms by which MCMs improve transfection, as well as coating compositions that impro

54 Live imaging and transfection assays for Arc overexpression were performe

55 Transient transfection assays of the mutant protein demonstrated a

56 s effective in inhibiting ICP0 expression in transfection assays than a host microRNA, miR-138, which

57 In yeast and cell-culture transfection assays, M156 only inhibited rabbit PKR but

58 ef exert anti-tetherin activity in transient-transfection assays, mainly Vpu promotes RBF206 release

59 though miR-H2 can repress ICP0 expression in transfection assays, such repression is weak.

60 widespread application of the INENI in cell transfection assays.

61 tial of this approach, the low and transient transfection associated with the large size of therapeut

62 Employing a transient transfection-based approach to rescue TSC2 function in m

63 The low-frequency mosaic pattern of transfection-based CRISPR/Cas9 delivery faithfully recap

64 cape which is not achievable using exogenous transfection-based luciferase reporter assays.

65 Here we show transfection-based multiplexed delivery of CRISPR/Cas9 t

66 Loss of Crk and CrkL induced by synCre transfection blocked cell proliferation and caused shrin

67 This indicates that not transfection, but rather translation being the major roa

68 generation (G0 and G1) dendrimers failed as transfection carriers.

69 dation of the outer ZN matrix and release of transfection competent CS/DNA NPs occurred in simulated

70 f endothelial cells by small interfering RNA transfection could shape the nature of the host response

71 lease degradation, exhibiting no significant transfection decay.

72 miR-143 and miR-145 transfection decreased cervical cell number by increasin

73 atment and tandem mCherry-EGFP-LC3B reporter transfection demonstrated that the autophagic flux was u

74 ried PBAE NP formulations were evaluated for transfection efficacy and cytotoxicity to a range of hum

75 e highest plasmid DNA binding capability and transfection efficacy of 88%, higher than those reported

76 er-to-DNA weight-to-weight ratio led to high transfection efficacy to all of the liver cancer lines,

77 ether overexpression of NC1 domain with high transfection efficacy would perturb spermatogenesis, in

78 id DNA vectors has significant impact on the transfection efficacy.

79 demonstrating 2 to 126-fold higher in vitro transfection efficiencies of different cell types in com

80 ll retained their biophysical properties and transfection efficiencies.

81 rnary nitrogen centers) in modulating the DC-transfection efficiencies.

82 drug delivery mechanisms have increased the transfection efficiency aiding in greater therapeutic re

83 hed structures can significantly improve the transfection efficiency and safety of PAEs highlighting

84 scale devices suffer from the unsatisfactory transfection efficiency and/or cell viability due to the

85 In vivo, transfection efficiency of a plasmid co-expressing hSef-

86 Transfection efficiency peaked at ~10% at 8h post sonica

87 es with nucleic acids and provided very high transfection efficiency with all nucleic acids and cell

88 MCMs), we observed up to 4-fold increases in transfection efficiency with simultaneous reductions in

89 owed efficient transfection of plasmids (40% transfection efficiency) and short interfering RNA (inte

90 The high transfection efficiency, controlled dosage delivery and

91 ding potential injury to DRG neurons and low transfection efficiency, respectively.

92 and time-dependent manner, resulting in high transfection efficiency.

93 a 'double-shell' miRNA distribution and high transfection efficiency.

94 pler structure, better stability, and higher transfection efficiency; therefore it may become a novel

95 rticle tracking microscopy, and quantitative transfection-efficiency assays on live cells to unveil t

96 S treatment after FIG4-small interfering RNA transfection enhanced neural differentiation, survival,

97 unctional characterization were performed in transfection experiments by using immunoblotting and imm

98 ZO-1 small interfering RNA and cDNA transfection experiments emphasized regulation of CXCL8/

99 ar to destabilise the wildtype protein in co-transfection experiments in a human RPE cell line.

100 tically active state in vitro, thus enabling transfection experiments that altered gene expression to

101 Transfection experiments using fluorescent protein and l

102 with detailed instructions for cell culture, transfection, fixation, heavy-metal staining, embedding

103 UB-GFP plasmids and for at least 5 days post-transfection for cells transfected with pEYFP-Mitotrap.

104 Acoustic-transfection has advantages over typical sonoporation be

105 the least because genetic deletion and cell transfection have led to contradictory data.

106 herapy of airway disorders where plasmid DNA transfections have so far proven inefficient in clinical

107 factor receptors and RET (rearranged during transfection) have been used when a systemic therapy is

108 addition, genetic associations and transient transfection identified PBF as a downstream target of th

109 ed macrophages (BMDMs), CLIC1 or CLIC4 siRNA transfection impaired transcription of IL-1beta, ASC spe

110 erum, they produced transformed foci without transfection in 10% CS, but not in 2% CS.

111 ther used to achieve efficient and selective transfection in dual-receptor expressing cancer cells.

112 The approach afforded efficient transfection in primary human fibroblasts as well as mes

113 as well as coating compositions that improve transfection in three-dimensional cell constructs.

114 of Wnt5a signaling by small interfering RNA transfection in vitro or by use of inhibitor of Wnt prod

115 with CS/DNA NP cores successfully mediating transfection in vitro.

116 normalities, which improved with anti-miR-29 transfection in vitro; endothelial-like cells derived fr

117 nes tested in vitro, confirmed effective DNA transfection in vivo.

118 Transient co-transfections in Arabidopsis protoplasts showed that A-Z

119 In eGFP transfections in vitro both the mean fluorescence intens

120 derived miR-150-containing EVs or premiR-150 transfection increased vascular endothelial growth facto

121 unocytochemical, and immunoblot analyses and transfection, infection, DNA synthesis, apoptosis, migra

122 ntin expression was upregulated upon MT1-MMP transfection into cells.

123 damaging the pSP189 plasmid followed by its transfection into human 293T cells to allow replication

124 and reconstituted infectious virus following transfection into mammalian cells.

125 Here, we show that AKR1B10 transfection into normal human keratinocytes reproduced

126 This MCM-based transfection is an advancement in gene delivery technolo

127 showed that polymeric nanoparticle-mediated transfection led to robust up-regulation of TRAIL in hAD

128 tion achieve the highest, safe non-viral DNA transfection levels (up to 54%) reported so far for prim

129 nd amenable to in vitro applications such as transfection, luciferase assays, immunofluorescence, wes

130 ich Mb expression was suppressed by Mb-siRNA transfection (Mb vector transfected vs. Mb vector, contr

131 Using Polyplus in vivo-jetPEI as a transfection medium with high efficiency for CRB3 KD in

132 Established transfection methodology often uses commercial reagents,

133 ata generated using non-isogenic strains and transfection models suggest that variation surrounding t

134 kinase (ILK), EGFR and NF-kappaB, as well as transfection of a dominant-negative mutant of Ras (RasN1

135 In human embryonic kidney HEK293 cells, the transfection of a duplex HBoV1 genome initiates viral DN

136 In this work, we studied gene transfection of a human prostate cancer cell line expose

137 Transfection of a miR-134 mimic repressed translation of

138 erfibrinolytic state in mice by hydrodynamic transfection of a plasmid encoding for tissue-type plasm

139 the autophagic flux in cardiomyocytes after transfection of a precursor miRNA library consisting of

140 ed MCT1 expression using adenovirus-mediated transfection of a shRNA into the third ventricle, transd

141 In rescue experiments, the transfection of a vector encoding for the active Notch i

142 respond in this manner to hypoxia or ATP but transfection of A2b restores this response, that Epac1 i

143 ivity, chemical stimulation of glycolysis or transfection of active Akt1 or Pdpk1 (phosphoinositide-d

144 Furthermore, transfection of an miR-130 inhibitor in preadipocytes en

145 Simultaneous transfection of an RKIP expression construct lacking the

146 Similarly, transfection of AP-2alpha cDNA decreased TACE promoter l

147 In contrast, transfection of AP-2alpha small interfering RNA increase

148 A20 promoter activity was up-regulated after transfection of AP1 cDNA in cells.

149 Transfection of CD4- 293T cells with a cDNA expression l

150 In a three-dimensional culture model, transfection of CEACAM1 into MCF7 breast cells can resto

151 Transfection of cell lines with ZNHIT3 expression vector

152 lization of this system requires a single co-transfection of cells with assay vectors, thereby enabli

153 Transfection of cells with ClC-3 siRNA decreased the exp

154 Transfection of cells with full-length CaM slightly incr

155 the cell lines tested, and it increases upon transfection of cells with TspanC8 tetraspanins such as

156 Following transfection of cells with UnaG and infection with wild-

157 Transfection of CREB in HEK293 cells together with the k

158 Transfection of cultured neurons with human GRIN2D cDNA

159 On transient transfection of diverse human and rodent cell lines, the

160 rials with origins as materials for cellular transfection of DNA and small interfering RNAs (siRNAs)

161 ethylation of the genome that was rescued by transfection of DNMT1.

162 Transfection of equal mass amounts of DNA in mouse lungs

163 ith double stranded DNA and promote in vitro transfection of eukaryotic cells.

164 Conversely, transfection of exosomes from subjects without ED with a

165 Consistent with that hypothesis, perinatal transfection of hair cells in KO-TgAC1 mice with a singl

166 Stable transfection of HEK-293T cells with plasmid DNA carrying

167 ease in permissiveness was blocked either by transfection of HEK-EqCXCL16 cells with small interferin

168 Transfection of HEK293 cells with the duplex DNA genome

169 Following transfection of HEK293 cells, the minimal requirements f

170 Upon transfection of HEK293 cells, we found that mutant RHOBT

171 Transient transfection of HEK293T cells with Wt (Wild type) hFOXA2

172 confers higher specificity even than plasmid transfection of HF-BE3, while maintaining comparable on-

173 This included viral transfection of hippocampal interneurons with channelrho

174 Transient transfection of human embryonic kidney 293 cells with th

175 Transfection of human PBMC's with TTSuV1 genomic DNA res

176 5 viral peptide target were examined by mRNA transfection of human peripheral blood lymphocytes (PBLs

177 Gene transfection of human pulmonary microvascular endothelia

178 Using biolistic transfection of individual RGCs and multielectrode array

179 In vivo adenoviral transfection of KLF2 to the carotid bodies in CHF rabbit

180 Escherichia coli, Citrobacter rodentium and transfection of LPS, AIM2 activators Francisella novicid

181 ed hPXR mRNA and protein expression, whereas transfection of LS180 cells with an miR-18a-5p inhibitor

182 Transfection of LS180 human colorectal adenocarcinoma ce

183 ng cell extracts from HeLa cells, as well as transfection of luciferase replicons in two types of car

184 r activity and validated its target mRNAs by transfection of Luciferase reporter plasmids into Huh7,

185 air by demonstrating the acoustophoretic DNA transfection of mammalian cells.

186 Transfection of MCs with mimic or inhibitor of miR-4443

187 The transfection of miR-146a mimic with PF6 was more efficie

188 Transfection of miR-146a/b suppressed and inhibition enh

189 Overexpression of miR-21 by transfection of miR-21 mimics into LMP1-transformed cell

190 The transfection of miR-92a-2-3p into the noDICE cell line f

191 Transfection of miR-exon4 mimic to the LS8 cells also si

192 Following transfection of MSCs with the mms6 gene there is bio-ass

193 Transfection of mutant CARD11 expression constructs into

194 Therefore, heterozygous transfection of native (WT) and p.T152HfsX180 hERG chann

195 E7 but not E6 transfection of NOK cells led to hypermethylation of a p

196 en EP2 receptor expression was normalized by transfection of NP-AERD fibroblasts.

197 uptake efficiency and less cell death in the transfection of oligonucleotides.

198 Our results show that upon successful transfection of Panc-1 cells, the exosome content was al

199 n of both ligand classes, nor with exogenous transfection of partner receptors (nucleotide P2Y12, cys

200 mineral coating improves microparticle-based transfection of plasmid DNA lipoplexes in several primar

201 nding neutrophil life span allowed efficient transfection of plasmids (40% transfection efficiency) a

202 iant translocation 1 (PVT1) was inhibited by transfection of primary ASMCs with small interfering RNA

203 r hydrogels release intact 3LM for efficient transfection of primary macrophages.

204 Here we show that transfection of purified in vitro generated circRNA into

205 Transfection of S631G variant into a 3-dimensional organ

206 Transfection of Sirtuin-1 small interfering RNA prevente

207 We show that a single transfection of small interfering RNA targeting class II

208 an efficient cell culture infection system, transfection of stool-isolated NV RNA into mammalian cel

209 Transfection of such motifs from Meg3 RNA, termed triple

210 We observed heterogeneous, low-level transfection of the collecting duct, proximal tubule, di

211 Transfection of the Fig4(Cys486Ser) cDNA into cultured F

212 mary, these data demonstrate effective siRNA transfection of the injured arterial wall and provide a

213 significantly increased ultrasound-mediated transfection of the murine brain when compared to commer

214 with the MEK1/2 inhibitor UO126 or following transfection of the non-phosphorylatable hRXRalpha Ala-2

215 cient, localized transfection and allows for transfection of three-dimensional cell constructs.

216 Transfection of TNF-alpha-treated mouse aortas and cultu

217 Vacuolization of fibroblasts was rescued by transfection of wild-type VAC14 cDNA.

218 in luciferase assays, an effect mimicked by transfection of YY1 siRNA.

219 d protocols for, and demonstrated successful transfection of, the free-living kinetoplastid flagellat

220 compared minicircle DNA with plasmid DNA in transfections of airway epithelial cells.

221 romoter from nucleotides (nt) -318 to +54 by transfections of luciferase reports containing different

222 Triple transfections of the dopamine receptor subtype and Gbeta

223 expression of p21(WAF1/CIP1), either by cDNA transfection or clinical drugs, preferentially impairs t

224 can be achieved by electroporation, chemical transfection or Lipofection of the transposase:DNA mixtu

225 Prostaglandin E Receptor EP1 transfection or treatment with EP1 agonist mimicked the

226 a commercial transfection agent with similar transfection performance but poor protection capability

227 of HBEC30-KT normal cells during the initial transfection period.

228 We describe novel CRISPR/Cas9 transfection plasmids and approaches for the speedy, sta

229 racterized by cryo-TEM experiments and their transfection potency was evaluated using different cell

230 roject of Ex-vivo Vein Graft Engineering via Transfection (PREVENT) IV trial participants who underwe

231 Additionally, the efficiency of the transfection procedure is compared to standard, containe

232 rk into a high proportion of cells, a hybrid transfection procedure was developed that involves elect

233 to improve production yields in a transient transfection production of a Sendai virus F/HN-pseudotyp

234 down of Notch3 expression by transient siRNA transfection promoted the expression of osteogenic trans

235 We established a transfection protocol allowing persistence of these 2 mi

236 e generated from relatively modest scales of transfection, providing a clear pathway to genome-scale

237 nt size into cells in culture, yielding high transfection rates and minimal cytotoxicity.

238 nter pediatric T-ALL patient cells without a transfection reagent and induce Plk1 knockdown on both p

239 taken up by cells in the presence of a lipid transfection reagent.

240 s well as greatly out-performing the leading transfection reagents SuperFect and the "gold-standard"

241 At 72 h after transfection, reduced DNMT3a expression, but not DNMT1 o

242 analytical techniques and by comparisons of transfection results.

243 Rearranged during transfection (RET), a receptor tyrosine kinase that is a

244 ss of function experiments by cDNA and siRNA transfection revealed profound effects of 3-OST3A expres

245 ng QIseq to uncloned parasites shortly after transfections revealed multiple insertions in mixed popu

246 ofection and electroporation methods, plasma transfection showed a better uptake efficiency and less

247 sion in pollen via antisense oligonucleotide transfection significantly reduced the expression of its

248 em cells for both two- and three-dimensional transfection strategies.

249 However, all of the transfection studies (over 2350 PAEs) have been limited

250 Cell transfection studies demonstrated a dominant-negative ef

251 ut protein-dependent yeast growth assays and transfection studies in the HT29 human CRC cell line to

252 Transient-transfection studies revealed that UL116 is efficiently

253 Transfection studies using mature miR mimics of miR-490

254 ned for eukaryotes, (2) a novel microfluidic transfection system employing hydrodynamically-controlle

255 An ideal nucleic-acid transfection system should combine the physical and chem

256 of this near-monoclonal TCR in a Jurkat cell-transfection system validated fine DYS specificity.

257 Using a PDE4B overexpressed lentivirus transfection system, we suggested that miR-124-3p suppre

258 i-fluorescence microscope, entitled acoustic-transfection system.

259 ells, providing the evidence of the acoustic-transfection technique for precise genome editing using

260 We introduce a new transfection technique that utilizes high frequency ultr

261 and local intracellular delivery to acoustic-transfection technique.

262 Single cell transfection techniques are essential to understand the

263 There have been limited transfection techniques that can deliver multiple types

264 ons of CAP beyond those provided by standard transfection techniques.

265 We used transient transfection to test the ability of ToxCast chemicals to

266 ly blocked matrix contraction after a single transfection treatment.

267 Thus, in pairwise transfections, UL16 binding is enabled only when the CTD

268 s over typical sonoporation because acoustic-transfection utilizing ultra-high frequency ultrasound o

269 lymer, polyethylene glycol, can be used as a transfection vector by forming a brush polymer-DNA conju

270 cells was either achieved via incubation or transfection via DNA labeling.

271 In contrast, the uptake of 25 nm GNPs by transfection was approximately four times higher after 2

272 Acoustic-transfection was further demonstrated to deliver CRISPR-

273 livery of 3 kDa dextran induced via acoustic-transfection was manifested by diffused fluorescence thr

274 a delivery vehicle and thus repetitive daily transfection was required because of mRNA degradation.

275 Plasmid transfection was used to modulate transforming growth fa

276 ssion of hsp60, achieved by in vitro plasmid transfection, was then performed to determine whether th

277 Using HIV-lentivector and plasmid DNA transfection, we also developed A2A and A2B receptor ove

278 Cardiomyocyte transfection with 11beta-HSD2 siRNA abolished the aldost

279 that produces reporter virus particles upon transfection with a GFP replicon plasmid.

280 ethanol-fed rats, but treatment with HA35 or transfection with a miR291b hairpin inhibitor restored T

281 Microtubule disruption or co-transfection with alpha-synuclein A53T, a PD-associated

282 n were observed as follows: both required co-transfection with alpha1 and beta2 subunits for maximal

283 ed in receptor-negative HEK-293T cells after transfection with an infectious clone of GFP-PRRSV.

284 We observed that transfection with an ITGA4-mRNA construct bearing a conv

285 tion of NADH, reduced expression of CtBP, or transfection with an NAD(H) insensitive CtBP, and are re

286 HeLa cell transfection with c-Jun and c-Fos increased CD177 mRNA.

287 Treatment with doxycycline and transfection with guide RNA (gRNA), donor DNA and piggyB

288 FN-responsive cells with type I IFN Notably, transfection with long dsRNA specifically vaccinates IFN

289 Notably, transfection with MAP17 did not change the quantity of S

290 orced expression of miR-142-3p via transient transfection with miR-142-3p mimic inhibited cell-to-cel

291 Transient transfection with miR-146b mimic and western blotting wa

292 Transfection with mmu-miR-155 and mmu-miR-2137 did not m

293 primary cells with HSV or CMV, or transient transfection with naked plasmid DNA, HIRA re-localizes t

294 lar stomatitis virus and Sendai virus and to transfection with poly(I.C).

295 Co-transfection with shRNA vectors against HK1, HK2, and HK

296 The results reveal heterogeneity of cell transfection with siRNA and outline disparity in RNA int

297 This effect was inhibited by transfection with siRNA anti-STAT3, suggesting the invol

298 s carrying floxed alleles of Crk and CrkL by transfection with synthetic Cre mRNA (synCre).

299 MSCs are genetically modified by transfection with the mms6 gene derived from Magnetospir

300 Monocyte transfections with pre-miR-124a and/or -125a caused redu