ライフサイエンスコーパス: transfection efficiency

1 nthesized and evaluated for DNA delivery and transfection efficiency.

2 role in DNA delivery and in determining the transfection efficiency.

3 and time-dependent manner, resulting in high transfection efficiency.

4 been proposed to be a major reason for poor transfection efficiency.

5 litating DNA release and leading to superior transfection efficiency.

6 -viral gene vectors from having an effective transfection efficiency.

7 dothelial cells and permitted calculation of transfection efficiency.

8 regulation of reporter genes or to normalize transfection efficiency.

9 that these could have significant effects on transfection efficiency.

10 r use has been limited by the relatively low transfection efficiency.

11 ectrophoresis and loss of transformation and transfection efficiency.

12 a 'double-shell' miRNA distribution and high transfection efficiency.

13 g in vivo cardiac gene transfer has been low transfection efficiency.

14 lutinating virus of Japan-liposome with >95% transfection efficiency.

15 atio to determine if either variable affects transfection efficiency.

16 es into immunocompetent mice without loss of transfection efficiency.

17 luciferase construct was used to control for transfection efficiency.

18 thus explaining the synergistic increase in transfection efficiency.

19 nactivated adenovirus significantly enhances transfection efficiency.

20 monstrated compared to controls despite a 1% transfection efficiency.

21 iposome, lipofectamine, further enhanced the transfection efficiency.

22 brane played a secondary role in determining transfection efficiency.

23 a suspension at 4 degrees C with no loss in transfection efficiency.

24 t cellular uptake and significantly enhanced transfection efficiency.

25 ficulties of nucleic acid packaging and poor transfection efficiency.

26 on, resulting in significantly improved gene transfection efficiency.

27 membrane rupture and therefore enhances the transfection efficiency.

28 ative to electroporation with an increase in transfection efficiency.

29 morphology, epithelial barrier function and transfection efficiency.

30 ta potential, as well as cellular uptake and transfection efficiency.

31 ed lipid displayed optimal peptide-targeted, transfection efficiency.

32 n (Fluc-eGFP) for noninvasive measurement of transfection efficiency.

33 output that is independent of cell number or transfection efficiency.

34 ll retained their biophysical properties and transfection efficiencies.

35 rnary nitrogen centers) in modulating the DC-transfection efficiencies.

36 eps might be manipulated in order to improve transfection efficiencies.

37 apy due to their multifunctionality and high transfection efficiencies.

38 escent intensity 85.3 [SD 48.5]; and average transfection efficiency 23.3% [range 10.6-30.9]).

39 (-s-s-) derivative (16 kDa) showed excellent transfection efficiency: 3.6 times higher than branched

40 the following conditions appear to optimize transfection efficiency: a DNA:DOTAP ratio of 1:6; a 24

41 h Chol at a 1:1 molar ratio gave the highest transfection efficiency after intravenous administration

42 drug delivery mechanisms have increased the transfection efficiency aiding in greater therapeutic re

43 Ad5RGDpK7 exhibited higher transfection efficiency, allowing a significant reductio

44 nversion frequencies reaching 30-70% at high transfection efficiencies and approximately 2% at low tr

45 the use of only two plasmids, which enhances transfection efficiencies and hence vector production.

46 Transfection efficiencies and transgene expression kinet

47 Transfection efficiencies and VEGF inhibition in LNCaP a

48 ther, functionalized nanoparticles exhibited transfection efficiencies and VEGF inhibition significan

49 r in vivo gene therapy due to their targeted transfection efficiency and ability to penetrate tissues

50 te an increase of 3-5 orders of magnitude in transfection efficiency and are potent activators of den

51 vivo delivery without compromising in vitro transfection efficiency and are thus promising carriers

52 Comparative analyses of transfection efficiency and cell viability for primary a

53 ndomly distributed cells, leading to limited transfection efficiency and cell viability, especially f

54 Despite superior transfection efficiency and cell viability, high cost of

55 Transfection efficiency and cellular internalization of

56 thods, we screened this library in vitro for transfection efficiency and cytotoxicity.

57 MAM dendrimer complexes can be used for high transfection efficiency and effective targeting of APCs

58 ize as a potential limiting factor affecting transfection efficiency and hence the influenza viral yi

59 This heterogeneity contributes to the low transfection efficiency and instability of cationic lipi

60 composition of acetals showed high in vitro transfection efficiency and low cytotoxicity in the deli

61 PEI (-s-s-) polyplexes showed higher transfection efficiency and lower cytotoxicity compared

62 outcomes depend on a proper balance between transfection efficiency and polyplex-induced cytotoxicit

63 eased incubation time up to 45 min increased transfection efficiency and reduced RI, but longer incub

64 hed structures can significantly improve the transfection efficiency and safety of PAEs highlighting

65 postulate that the relationship between the transfection efficiency and the ac frequency is determin

66 a demonstrate a correlation between in vitro transfection efficiency and the combination of several p

67 ted alkaline phosphatase gene to control for transfection efficiency and the effects of culture condi

68 Transfection efficiency and toxicity of PEI are highly d

69 shown to have an important influence on the transfection efficiency and toxicity of the polyplexes.

70 ncubation time, and AS-ODN concentrations on transfection efficiency and toxicity.

71 lthough the cellular uptake was similar, the transfection efficiency and VEGF expression of PAM-ABP u

72 scale devices suffer from the unsatisfactory transfection efficiency and/or cell viability due to the

73 owed efficient transfection of plasmids (40% transfection efficiency) and short interfering RNA (inte

74 on methods are available, cell toxicity, low transfection efficiency, and high cost remain hurdles fo

75 bility, superoxide production, cytotoxic RNA transfection efficiency, and measurement of SOD2 protein

76 val of transfected viral DNA was measured as transfection efficiency, and mutagenesis at the lesion s

77 Survival effects were determined from transfection efficiency, and mutation fixation at the le

78 Survival of the M13 DNA was measured as transfection efficiency, and mutation fixation at the le

79 c responses of signal transduction pathways, transfection efficiency, and subcellular localization of

80 initely, can be concentrated with no loss in transfection efficiency, and the toxicity levels can be

81 Targeted gene delivery, transfection efficiency, and toxicity concerns remain a

82 quency of up to 11.9% and when corrected for transfection efficiency approached 43%.

83 The PA:LFn-GAL4:ASO complexes had transfection efficiency approximately equivalent to Nucl

84 Transfection efficiency, as measured by luciferase prote

85 Their transfection efficiency, as well as that of the lamellar

86 rticle tracking microscopy, and quantitative transfection-efficiency assays on live cells to unveil t

87 zed defect in gene expression nor an altered transfection efficiency, because the islet amyloid polyp

88 However, uncertain transfection efficiency becomes a bottleneck, especially

89 hanically tailored complex which may enhance transfection efficiency by controlling the stability of

90 We have improved the transfection efficiency by means of recombinant adenovir

91 Transfection efficiency by PEG-modified, cleavable RTNs

92 The in vivo enhancement of transfection efficiency by this modified gene delivery v

93 x concentration at the cell surface enhanced transfection efficiency by up to 8.5-fold over the best

94 ace composition on vector immobilization and transfection efficiency can also be studied.

95 ion potential, primarily aiming at increased transfection efficiency, cell selectivity and reduced cy

96 hat targeted and stabilized particles retain transfection efficiencies comparable to the nonstabilize

97 Our new gene carrier exhibits high gene transfection efficiency, comparable to or even better th

98 ble to reducing agents, resulting in greater transfection efficiency compared to ABP alone.

99 The high transfection efficiency, controlled dosage delivery and

100 pecific immunoliposome nanocomplex with high transfection efficiency could overcome these limitations

101 Here, we investigated the transfection efficiency, cytotoxicity, osteogenic potent

102 Transfection efficiency data for the corresponding CD:pD

103 tinoic acid treatment resulted in nearly 50% transfection efficiency-defined as the proportion of tra

104 Variations in transfection efficiency, delivery-induced cytotoxicity a

105 Transfection efficiency depended upon the form of the li

106 emonstrate that MC can significantly improve transfection efficiency, duration of transgene expressio

107 neas with chloroquine and EDTA increased the transfection efficiency eight-fold and threefold, respec

108 n gene expression studies, especially in low transfection efficiency experiments; and (c) facilitatio

109 arations containing largely intact DNA; (ii) transfection efficiencies for the development of stable

110 ser energy and determined cell viability and transfection efficiency for both irradiation regimes.

111 tro transfection studies determined a higher transfection efficiency for each cyclic PEI sample when

112 Recently, unprecedented transfection efficiency for primary endothelial cells ha

113 trated to be the critical factor determining transfection efficiency for these polymers, mediating ef

114 tructural elements that generate the highest transfection efficiency for this new type of cationic li

115 ures as high as 9 atm leads to a increase in transfection efficiency from 1.7+/-.5 to 62+/-3.9% and a

116 Transfection efficiencies >95% were obtained by selectin

117 Maximum transfection efficiency (> 90%) requires a 2-hour incuba

118 High, stable transfection efficiencies in human ES cells have been di

119 Transfection efficiencies in isolated rat hepatocytes ap

120 -based gene delivery systems were tested for transfection efficiency in a variety of cell lines, incl

121 nverted hexagonal CL-DNA complexes show high transfection efficiency in cell culture.

122 PE at a 1:1 molar ratio mediated the highest transfection efficiency in cell culture.

123 imately an order of magnitude improvement on transfection efficiency in CFBE41o- cells.

124 As with previous systems, strong transfection efficiency in comparison with commercial st

125 Transfection efficiency in cultured cells was dependent

126 achieve a good balance between toxicity and transfection efficiency in gene delivery systems.

127 n:PEI at a weight ratio of 5:5 showed higher transfection efficiency in HEK293, 3T3 and PC3 cells tha

128 Transfection efficiency in HeLa and NIH/3T3 cells were c

129 ce protein (GFP), we achieved a sonoporation transfection efficiency in rate aortic smooth muscle cel

130 ery vectors) with alanine nearly doubles its transfection efficiency in the presence of serum and als

131 2-kDa PEI yields a nontoxic polycation whose transfection efficiency in the presence of serum is 400

132 the new multivalent lipid greatly increases transfection efficiency in the regime of small molar rat

133 of the aliphatic chain length (n = 12-18) on transfection efficiency in vitro was determined using ca

134 pNT in polyplexes prevented the reduction of transfection efficiency induced by a low temperature.

135 er, lack of efficient targeted delivery, low transfection efficiency, instability to nucleases, poor

136 Here, we tested the hypothesis that DNA transfection efficiency is limited by a simple physical

137 ratio) are important factors that determine transfection efficiency, lipid-DNA complex preparations

138 that encode secreted proteins, however, low transfection efficiency may not preclude bio-activity of

139 ical limit; new methods designed to increase transfection efficiency must increase DNA concentration

140 demonstrating 2 to 126-fold higher in vitro transfection efficiencies of different cell types in com

141 In this study, we evaluated transfection efficiencies of mRNA delivered in naked and

142 sts to CL-DNA complexes, where the optimized transfection efficiencies of multivalent and monovalent

143 possible strategies to significantly improve transfection efficiencies of synthetic gene vectors.

144 Transfection efficiencies of the modified BACs into huma

145 E2F2 and EGFP in corneal endothelium with a transfection efficiency of 10% to 12%, using the pIRES2-

146 Microporation also yielded a transfection efficiency of 93% and an average viability

147 In vivo, transfection efficiency of a plasmid co-expressing hSef-

148 ficient adenovirus mutant dl312 enhanced the transfection efficiency of a plasmid DNA-expressing beta

149 ) in combination with liposomes enhanced the transfection efficiency of cationic liposomes.

150 the past decade since they can maintain the transfection efficiency of commercially available, 25k b

151 an optimized transfection protocol in which transfection efficiency of Drosophila Schneider 2 cells

152 These peptides uniquely enhance the transfection efficiency of GFP-encoded plasmid DNA (pRmH

153 In contrast, the transfection efficiency of H(II)(C) complexes is indepen

154 d saturates for at a value rivaling the high transfection efficiency of H(II)(C) complexes.

155 stabilized, which significantly compromises transfection efficiency of materials shown to be effecti

156 Interestingly, the transfection efficiency of mDC2 with plasmid DNA vectors

157 an important prerequisite for improving the transfection efficiency of non-viral vector-mediated gen

158 e infection efficiency of adenovirus and the transfection efficiency of plasmid DNA in the presence o

159 oly(L-lysine) and protamine, can enhance the transfection efficiency of several types of cationic lip

160 histocultures, and promotes the delivery and transfection efficiency of siRNA-lipoplexes under the lo

161 The transfection efficiency of SN carrying a reporter gene w

162 This, and the high heterogeneity and low transfection efficiency of the Caco-2 cell line prompted

163 traction of plasma membrane cholesterol, the transfection efficiency of the gene delivered by dendrim

164 In comparison to transfection efficiency of the homologous expression pla

165 iated enhanced cell uptake and high in vitro transfection efficiency of the polyplexes were demonstra

166 The transfection efficiency of the stable complex in 15% fet

167 The DNA condensation ability and gene transfection efficiency of these LMWP peptides were test

168 present important factors in determining the transfection efficiency of this hybrid expression system

169 To further improve the transfection efficiency of this targeting complex, a syn

170 ss colloidal-osmotic swelling, we achieved a transfection efficiency of ~20%, regardless of the prese

171 he proposition that the strong dependence of transfection efficiency on the oxidation state of BFDMA,

172 achieve, especially for cell lines with low transfection efficiency or when expression of multiple g

173 forming denpol showed significantly improved transfection efficiency over Lipofectamine in serum-cont

174 The presence of HoKC increased the in vitro transfection efficiency over that of the original comple

175 Transfection efficiency peaked at ~10% at 8h post sonica

176 he Flt23k plasmid was evaluated by measuring transfection efficiency (percentage of cells expressing

177 However, until now, low transfection efficiency, poor tissue penetration, and no

178 , this histidine-rich tail markedly improved transfection efficiency, presumably by increasing the bu

179 of transfecting human cells in culture with transfection efficiencies ranging from 0.3% to 4.1%, whi

180 t of our effort to investigate the structure-transfection efficiency relationships of self-assembled

181 ffect of pore behavior on cell viability and transfection efficiency remain poorly elucidated.

182 ding potential injury to DRG neurons and low transfection efficiency, respectively.

183 The high transfection efficiency seen in the BHK cells allows stu

184 delivery, which overcome the problem of poor transfection efficiency seen with the plasmid-based syst

185 ion efficiencies and approximately 2% at low transfection efficiencies, simultaneous homozygous knock

186 gene delivery, albeit with unacceptably low transfection efficiencies (TE).

187 ed that BHH cell hybrids seem to have better transfection efficiencies than either of the parental ce

188 elded polyplexes afford up to 10-fold higher transfection efficiencies than the analogous stably shie

189 containing DOPE showed substantially higher transfection efficiency than those formulated with DOPC,

190 ruct, such as PAC DNA, substantially reduces transfection efficiency, the size effect of a DNA fragme

191 expression in COS-1 cells and correction for transfection efficiency, the Trp(173) allozyme displayed

192 pler structure, better stability, and higher transfection efficiency; therefore it may become a novel

193 th all of the cell lines used, regardless of transfection efficiency, time of processing posttransfec

194 The triethylphosphonium polymer shows transfection efficiency up to 65% with 100% cell viabili

195 sphate (P) ratio of 10 and characterized for transfection efficiency using human bone marrow stromal

196 The transfection efficiencies vary as a function of the PEI/

197 The transfection efficiency was 1.2% based on green fluoresc

198 Mean transfection efficiency was 89.0% (SD 1.9).

199 The transfection efficiency was also found to be LpDNA dose-

200 ight, and %contraction band necrosis assays; transfection efficiency was assessed using fluorescent m

201 Transfection efficiency was determined at 24 hours by nu

202 Transfection efficiency was determined with FITC-labeled

203 Transfection efficiency was determined with fluorescein-

204 Transfection efficiency was estimated as the percentage

205 Transfection efficiency was estimated by GFP expression.

206 Transfection efficiency was evaluated by green fluoresce

207 Transfection efficiency was further increased by additio

208 Transfection efficiency was maximized at rho = 0.4.

209 following a single administration, and their transfection efficiency was not attenuated by repeated a

210 High transfection efficiency was observed into both CD138(+)

211 Based on reporter plasmid expression, transfection efficiency was sixfold higher in ischemic m

212 for erythropoietin production and retroviral transfection efficiency, we chose to use HepG2 cells to

213 Their transfection efficiencies were at least two orders of ma

214 No discernible differences in transfection efficiencies were found between K-1735 clon

215 Transfection efficiencies were monitored by cotransfecti

216 reas fibroblasts, with up to 50-fold greater transfection efficiency, were less potent.

217 nt in pDNA loading, intracellular uptake and transfection efficiency, when compared to NPs lacking th

218 delivery strategy would facilitate improved transfection efficiency while eliminating the toxicity s

219 es with nucleic acids and provided very high transfection efficiency with all nucleic acids and cell

220 In contrast, transfection efficiency with DNA reaches its maximum at

221 a significant interest because of their high transfection efficiency with low toxicity.

222 MCMs), we observed up to 4-fold increases in transfection efficiency with simultaneous reductions in