ライフサイエンスコーパス: transformant

1 us increasing the utility of a single stable transformant.

2 e selected from an initial set of 26 primary transformants.

3 jected embryos and, therefore, the number of transformants.

4 n plated on a selective medium to screen for transformants.

5 have been biased because of the selection of transformants.

6 ity in liquid cultures was reduced in 83% of transformants.

7 ates several hundred and up to two thousands transformants.

8 cell line SIRC was used to establish stable transformants.

9 ental treatments, and studies of mutants and transformants.

10 BASTA-resistance gene for selection of plant transformants.

11 rium formation was restored in the resulting transformants.

12 ufficient to achieve a 50% yield of targeted transformants.

13 ll as the viability of knockout and knock-in transformants.

14 both P4-expressing NTHi and Escherichia coli transformants.

15 at an elevated rate in beta-lactam-selected transformants.

16 n(23F)-1, and selected beta-lactam-resistant transformants.

17 actam resistance might coselect for capsular transformants.

18 s increase the level of apoptosis in primary transformants.

19 n total cell culture or cell extracts of the transformants.

20 reased resistance observed in Golden Promise transformants.

21 cient hosts as efficiently as wild-type (WT) transformants.

22 RS mRNA also takes place in Escherichia coli transformants.

23 f a very bright red fluorescent protein into transformants.

24 al regulation of the HATS in the 35S::NRT2.1 transformants.

25 ze genetic diversity in the final 3.6x10(10) transformants.

26 coli, although at a low frequency of ~10(-3) transformants/10(6)cfu.

27 d repeat of the nos 3'-UTR, 51 of 56 primary transformants (91% of the population) showed highly effe

28 ride (AMCA) selection identified a resistant transformant able to grow in media containing 76 microg/

29 transform the biofilm-negative strain O35E; transformants able to form biofilms were identified and

30 HsNHA2 transformants accumulated less Li(+) than the salt-sensi

31 the serum-sensitive strain MC317, the MC317 transformant acquired serum resistance.

32 ing), does not require development of stable transformants, allows characterization of phenotypes tha

33 is deletion occurred in approximately 10% of transformants analysed and was stably maintained through

34 -qPCR showed reduced Spr1 mRNA levels in all transformants analysed, and these correlated with reduce

35 None of the transformants analyzed were true gbpB mutants, since the

36 Studies of CD74, CD44, and CD74-CD44 transformants and corresponding mutant cells showed that

37 or system requires minimal number of primary transformants and eliminates the need for crossing, whil

38 lection markers affect vector copy number in transformants and explain why blasticidin has become the

39 Using stable cell transformants and expression array analysis, we determin

40 y yielded higher numbers of zeocin-resistant transformants and higher levels of resistance than AR- o

41 d analyses of the progenies from the primary transformants and supertransformants, revealed that HDGS

42 or reliable visual detection of Burkholderia transformants, and carries a modified sacB gene that all

43 H region contained at least 10(6) individual transformants, and thus should represent a wide range of

44 precipitation, resulting in high copy number transformants; and electroporation, an effective techniq

45 in the NER-deficient hosts; therefore, these transformants are produced by plasmid bearing spontaneou

46 Homoplasmic plastid transformants are readily obtained in cell colonies, or

47 MLV) is a multistep process in which primary transformants are stimulated to proliferate but subseque

48 When fruiting was induced, highly-silenced transformant AS5 failed to colonize the compost, whilst

49 ntages were similar to those of 35S-mgfp5-ER transformants at 32 degrees C or 38 degrees C.

50 We developed stable transformant B16-F10 melanoma cell lines that express a

51 Transformants bearing the mutant gene were identified by

52 expanding leaves of both PetM and PetC RNAi transformants bleached rapidly and developed necroses, w

53 Both transformants brought about the total bioconversion of m

54 sis, and gene expression was demonstrated in transformants by northern-blot analysis.

55 We confirmed the clonality of selected transformants by Southern blot hybridization and PCR ana

56 re, we demonstrate that populations of these transformants can be enriched for strains that receive m

57 inucleate asexual spores formed from primary transformants can identify deletions of genes that are n

58 nvolving scores of genes, 90% or more of the transformants carried a single insertion of the transfor

59 Similarly, transformants carrying a PHOT transgene with both LOV do

60 ploiting segregation among the progeny of co-transformants carrying both the selectable marker gene a

61 with untransformed control cells, malignant transformants carrying the activating erbB-2/neu mutatio

62 Transformants carrying the mutated gene were more resist

63 Sorghum transformants carrying this cassette, were not improved

64 to 6-fold increase in its level in all four transformant cell clones.

65 When the resultant transformant cell lines are introduced in turn into the

66 The chlorophyll content of the transformant cells expressing the ChlB variant from the

67 irradiance was much more rapid in the rwt43 transformant compared with plants containing ADP-sensiti

68 nder a fluctuating light regime by the rwt43 transformant compared with wild-type Arabidopsis suggest

69 fficiency and more than 4-fold the number of transformants compared with TA cloning.

70 dditional analysis revealed that each barley transformant contained a range of different mutations, i

71 Surprisingly, piperacillin-resistant transformants contained no alterations in PBP genes but

72 While pBSV26 transformants contained this incompatible vector, the ve

73 w irradiance except in the Arabidopsis rwt43 transformant containing an ADP-insensitive Rca.

74 Four transformants containing lp28-1 induced severe arthritis

75 we examined approximately 3,000 B. anthracis transformants containing pUTE29-plcR-papR and found a si

76 Swimming speed is rescued in bop5-1 transformants containing the wild-type IC138, confirming

77 na leaves were infiltrated with a mixture of transformants containing wild-type B1 (wtB1) plus wtB2 p

78 virulent Breinl strain, suggesting that this transformant could serve as a nonrevertible, attenuated

79 nt transformants, no single-copy efficacious transformants could be found.

80 enic determinants, we evaluated C. muridarum transformants deficient in the plasmid-borne gene pgp3,

81 These transformants demonstrate that plastid RNA editing can b

82 Characterization of the mutant and transformant demonstrated that LrrA is associated with t

83 ays a role in Ab-MLV transformation, primary transformants derived from Arf(-/-) and p16(Ink4a(-/-))

84 th YFP permitted quick and easy screening of transformants, detection of apoplastic localization, and

85 s cloned into Escherichia coli JM109 and the transformant developed increased U(VI) resistance and th

86 than vector controls, and the antisense sigA transformant did not grow.

87 ation vectors, selection and regeneration of transformants, evaluation of transgene integration and i

88 ed in the generation of multiple independent transformant events exhibiting dramatically reduced sorg

89 An infectivity study revealed that all nine transformants examined, each of which represented one of

90 ired mutation being found in essentially all transformants examined.

91 The resultant O35E transformant exhibited a serum-sensitive phenotype.

92 ations of OMT, the progeny of omr1-1 initial transformants exhibited a bushy phenotype at the rosette

93 Primary transformants exhibited poor seed set, but homozygous li

94 f S degrees and, under these conditions, the transformants exhibited wild-type growth and H(2) produc

95 ive phase during which highly viable primary transformants expand, followed by a period of marked apo

96 and even though all p16(Ink4a(-/-)) primary transformants experienced crisis, these cells became est

97 in LS biogenesis were created: a chloroplast transformant expressing a truncated and unstable LS poly

98 we successfully generated a Pichia pastoris transformant expressing and secreting apidaecin.

99 ild-type or C56S/C242S enzymes but not for a transformant expressing the C150S enzyme.

100 ild-type or C56S/C242S enzymes but not for a transformant expressing the C150S enzyme.

101 The transformants expressing CPAF mutated at intermolecular

102 genes and is achieved via generating stable transformants expressing dsRNA in planta.

103 Transformants expressing green fluorescent protein were

104 n of LMWgs and gliadins was studied using 20 transformants expressing hairpin RNA in their endosperm.

105 Escherichia coli transformants expressing hRes could be rescued from ther

106 died the expression phenotypes of Drosophila transformants expressing mini-white transgenes in which

107 In transformants expressing MoMCM1-eGFP fusion, GFP signals

108 Transformants expressing OXIDATIVE STRESS 3 (OXS3) or OX

109 n increased protection from apoptosis in Abl transformants expressing Pim kinases.

110 The intracellular cAMP level was reduced in transformants expressing the MST7(S212D T216E) allele.

111 in the vegetative hyphae and appressoria of transformants expressing the MST7(S212D T216E) allele.

112 d sensitivity to DMI fungicide tebuconazole, transformants expressing the mutated or the wild type Vv

113 The UV-generated mutant as well as transformants expressing the VvCYP51/Y137H did not exhib

114 ide bond was also observed in vivo for yeast transformants expressing the wild-type or C56S/C242S enz

115 Transformants expressing tri5 displayed low chitinase ac

116 ionary phase for the parental strain and for transformants expressing wild-type or C56S/C242S enzymes

117 In v-Abl transformants, expression of Pim-1 and Pim-2, but not Pi

118 However, appressoria formed by these transformants failed to penetrate and infect rice leaves

119 efficacy of the system in recovering cotton transformants following Agrobacterium-mediated transform

120 bilizes transgenic plastids in heteroplasmic transformants following antibiotic withdrawal, enhancing

121 After screening a single primary transformant for each line, clones associated with mutan

122 id preparations from the isolate generated a transformant for which the MICs of all of the carbapenem

123 plied this strategy and examined field-grown transformants for both effects on wood biochemistry and

124 in each of the eight resulting categories of transformants for further physiological analyses.

125 ion vector, and screened approximately 7,500 transformants for gfp-ftsI alleles that failed to comple

126 ted to reliably generate libraries of >10(7) transformants from a pool of PCR products and a lineariz

127 either tlyC or pld resulted in the escape of transformants from endosomal vacuoles into the host cell

128 by PCR and compared with kanamycin-selected transformants from the same T(1) seed pool.

129 itional rice varieties and four T-DNA tagged transformants from the Taiwan Rice Insertional Mutant re

130 ining nine cytosines) was easily detected in transformants generated after infection of REF52 cells e

131 The resultant transformants generated avermitilol (2) as well as the d

132 fter screening over 600 hygromycin-resistant transformants generated by Agrobacterium tumefaciens med

133 We demonstrate that the transformants generated with this system exhibit the exp

134 In FGK3-GFP transformants, GFP signals mainly localized to the cytop

135 of the accD knockout was revealed in plastid transformants grown in the absence of antibiotics.

136 sis showed that, compared to RM118, the lex2 transformant had acquired a tetrasaccharide, Gal-alpha1,

137 CENH3 RNAi transformants had reduced fertility because of frequentl

138 n a population of independent tomato primary transformants harboring a stably integrated polygalactur

139 xpression shuttle system, 78 growth-retarded transformants harboring antisense DNA fragments were als

140 tern blots of cell extracts from clostridial transformants harboring plasmid constructs of thlP-sNTR

141 Luciferase is induced to maximum levels in transformants harboring the full-length RAD51-luciferase

142 t in membrane vesicles from Escherichia coli transformants have not yielded strong enough signals for

143 Forty-seven transformants having motility defects were characterized

144 ions typically used for selection of nuclear transformants, herbicides caused rapid disintegration of

145 Screening 16 822 bacterial transformants identified 110 terminator mutants, most of

146 psbS gene and analysed CO(2) assimilation in transformants in a fluctuating measurement light regime.

147 ific chromosome damage, and the frequency of transformants in Hsp70.1/3(-/-) cells in comparison to H

148 lasmid bearing the model ICL failed to yield transformants in NER-deficient host cells, proving the s

149 was still down-regulated in the 35S::NRT2.1 transformants in response to repressive nitrogen or dark

150 The authentic ICLs yielded transformants in the NER-deficient hosts; therefore, the

151 aporthe oryzae, allowing rapid generation of transformants in which individual genes have been silenc

152 oculated with T. virens Gv29-8 wild type and transformants in which SM1 was disrupted or constitutive

153 Phenotype characterizations of the resulting transformants indicate that the SAM domain but not the R

154 xamination of the leaves from the T3 of RNAi transformants indicated reduction of cell expansion in v

155 ing revealed single-site integration in each transformant, indicating that insertional mutagenesis is

156 VOCs released by the tri5-transformant induced expression of tomato defence genes

157 Primary barley co-transformants involving hpt::gfp (the selectable marker)

158 faster when consuming inverted-repeat stable transformants (irAGO8) plants but did not differ from th

159 One of the remarkable aspects about the transformants is that they exhibit a higher level of res

160 hritis in SCID mice, in contrast to the five transformants lacking lp28-1.

161 esistant to tebuconazole compared to control transformants lacking the mutation, but the expression o

162 ts in membranes from Mrp and control E. coli transformants led to a hypothesis explaining how activit

163 able silencing of the GFP in the C. acutatum transformant line 10 expressing GFP derived from C71.

164 the same nanosensors in Arabidopsis thaliana transformants manifested transgene silencing and undetec

165 eumoniae resulted in excellent growth of the transformant MG-1655 (pAP1) on the glucose analog.

166 Interestingly, c-Myc plus Bcl-XL transformants mimicked squamous carcinomas, whereas H-Ra

167 The highest efficiency (4.3 x 10(8) Transformants/mug DNA) was obtained at the stationary gr

168 As only a dozen or less yeast transformants need to be screened to obtain a clone with

169 pite screening a large number of independent transformants, no single-copy efficacious transformants

170 he native plasmids, rendering the respective transformants noninfectious to mice.

171 only Arabidopsis beta-Rca, although not in a transformant of Arabidopsis that expresses a tobacco-lik

172 vesting antenna size3 (tla3) DNA insertional transformant of Chlamydomonas reinhardtii is a chlorophy

173 The stable transformant of HepG2, overexpressing HABP1 does not lea

174 00 (residues 1-1000) is secreted by a stable transformant of McA-RH7777 cells as a monodisperse parti

175 train ATCC 35405 and an lrrA gene expression transformant of strain ATCC 33520 were constructed.

176 in diploids between a wild-type strain and a transformant of the mutant containing an ectopic copy of

177 measuring chlorophyll content of dark-grown transformants of a chlB-lacking mutant of the cyanobacte

178 DNA insertional transformants of Chlamydomonas reinhardtii were screened

179 Suppression of MTP gene expression in stable transformants of McA-RH7777 cells by micro-interfering R

180 acterization of particles secreted by stable transformants of McA-RH7777 cells demonstrated the follo

181 he initial lipidation of apoB:1000 in stable transformants of McA-RH7777 cells.

182 Transformants of P. infestans stably silenced for Pibzp1

183 We recovered seven independent transformants of P. vittata and four independent C. thal

184 We generated transformants of the cyanobacterium Synechocystis sp. PC

185 KO gene disruption transformants of the U1 and U2 loci produced viable cell

186 n polystyrene surfaces in these strains, but transformants of these strains carrying a multiple-copy

187 Two independent transformants of three generations exhibited vigorous ro

188 On the other hand, progeny of transformants omr1-5 , omr1-7 , and omr1-8 had a normal

189 rtase-deficient yeast strain and plating the transformants on a medium containing sucrose as the sole

190 le tagged mutant populations, including TRIM transformants or Tos17 lines, were about 10-fold higher

191 ns, increasing efficiency from less than one transformant per cm(2) to over 21 transformants per cm(2

192 +/- 0.13 x 10(-6) and 4.33 +/- 0.78 x 10(-6) transformants per cell, respectively.

193 DNA delivery (UDD) was 9.8 +/- 2.3 x 10(-6) transformants per cell, which was nine times more effici

194 s than one transformant per cm(2) to over 21 transformants per cm(2).

195 is very efficient, yielding several thousand transformants per microgram of input DNA or conjugation

196 c libraries were of the order of 10(4)-10(5) transformants per microgram of insert, which is the same

197 ly large, the second step, a mutation to the transformant phenotype, becomes increasingly likely.

198 Approximately 15% of the transformant plants identified from both sense and antis

199 0-fold higher than the frequency of standard transformants, probably because mass production of embry

200 Western immunoblotting showed that transformants produced both wild-type MAA1 and MAA1Delta

201 sicles of antiporter-deficient E. coli EP432 transformants produced higher levels of secondary Na(+)(

202 by an average of 2-fold among 12 independent transformants, relative to vector-alone controls.

203 cassette combinations for use in A. suspensa transformants, resulting in two hybrid strains exhibitin

204 Analysis of several independent transformants revealed that the transformed cells exhibi

205 Analysis of 100 Pyr(+) transformants revealed that this recombination system co

206 ed into N. uncinatum, and in two independent transformants, RNAi significantly decreased lolC express

207 ensitive to ADP inhibition in an Arabidopsis transformant, rwt43, which expresses only Arabidopsis be

208 infected with electroporated C. burnetii and transformants scored as organisms replicating in the pre

209 From approximately 5000 transformants screened, 12 bacterial colonies were ident

210 gainst stem rust, and progenies from several transformants segregated in a 3:1 ratio for resistancesu

211 Analysis of co-transformants selected using a spectinomycin-resistant 1

212 ith its parent counterpart HepG2, the stable transformant showed enhanced tumorigenicity as evident b

213 The resulting transformants showed evidence for the presence and expre

214 In the presence of superoxide, sense sigA transformants showed greater resistance than vector cont

215 KI-ILK transformants showed increased sensitivity to EGFR inhib

216 Analysis of the resulting transformants showed that TALEN-induced double strand br

217 Analysis of these transformants showed that the transcripts containing the

218 These transformants showed up to 85.6% suppression in DME tran

219 ongly repressed in young leaves of both RNAi transformants, showing that the M subunit is as essentia

220 In v-Abl transformants, SOCS-1 can inhibit cytokine signals, but

221 A libraries containing 3.5-5 x 10(5) primary transformants, starting with 5 mug of total RNA prepared

222 used to replace the uspA1 gene of O35E, this transformant strain did not form a biofilm.

223 The transformant strain, NR21, exhibited about 50% lower exp

224 This mutant combination yielded transformant strains with up to 40% of wild-type nitrate

225 edia with NAA, 49.0% +/- 3.9% of BA-mgfp5-ER transformants strongly expressed GFP; expression percent

226 esults illustrate the value of expanding AOX transformant studies to whole tissues.

227 rain but is unable to process it into viable transformants, suggesting a role for CoiA after DNA upta

228 inactivation of resT resulted in merodiploid transformants, suggesting that resT is required for B. b

229 B, with fungicide sensitivity testing of the transformants, suggests that both proteins are similarly

230 rmants were markedly altered with respect to transformants synthesizing wild-type Sultr1;2 protein.

231 analysis revealed that the biofilm-positive transformant T14 contained a hybrid O46E-O35E uspA1 gene

232 Transformant T14 was also shown to be unable to express

233 We have generated a Synechocystis PCC6803 transformant that expresses a fusion protein comprising

234 white light in stark contrast to recombinant transformants that are white.

235 hows structural specificity as D. discoideum transformants that expel chloroquine do not expel pipera

236 and the serine amber suppressor tRNA yielded transformants that grow on agar plates lacking uracil.

237 Consistent with these properties, Volvox transformants that harbor a glsA antisense transgene tha

238 nterestingly, for the same mutant mRNA, only transformants that produce Bop protein contain bop mRNA.

239 Transformants that survive this phase emerge as fully ma

240 y are absent, was transformed with lex2 DNA, transformants that were reactive with MAb 5G8 were obtai

241 types are dominant and are scored in primary transformants, this system does not require rounds of se

242 In this report, we used Oct-1 antisense transformants to determine that Oct-1 represses the inte

243 y photoelectrophysiological analysis of RNAi-transformants to mediate phototaxis signaling in Chlamyd

244 it had a strong effect on the ability of MT transformants to migrate or to fill a wound.

245 east Schizosaccharomyces pombe to select for transformants tolerant to cadmium.

246 When WI38 cells or its transformant VA13 cells were adhered to LN5 or FN, alpha

247 n (GEM) in WI38 cells versus their oncogenic transformant VA13 cells.

248 s thus two key roles: ensuring production of transformants via interaction with RecA and competence s

249 The transformant was cross-resistant to methyl N-(4'-(9-acri

250 A low copy transformant was propagated to the T4 generation and exa

251 scence of the purified GFP isolated from the transformant was quenched by myeloperoxidase (MPO)-gener

252 A small subset of transformants was consistent with assimilation of a sing

253 ransposon Tn4001mod, a mutant library of 110 transformants was constructed and all insertion sites we

254 The virulence of the resulting transformants was no different from that of the parent s

255 Surprisingly, the 5G8 reactivity of these transformants was phase variable, although the lex2 locu

256 r had one-sided homology, the DSB in most co-transformants was repaired using two DNAs, the donor and

257 media with NAA, 7.9% +/- 1.6% of BA-mgfp5-ER transformants weakly expressed GFP, similar to GCP cultu

258 Phenotypic changes in the stable transformant were associated with the increased "HA pool

259 into Escherichia coli Rosetta cells, and the transformants were able to grow and effectively transfor

260 spite increases in beta-oxidation, only ACS5 transformants were able to grow on oleate.

261 Transformants were analyzed by oligodeoxynucleotide hybr

262 Both transformants were characterized by lower levels of star

263 ts were used to transform chlamydiae and the transformants were characterized phenotypically and at t

264 The transformants were classified into nine groups based on

265 oter was used to express HopAI, PMIR1 -HopAI transformants were defective in the spreading of invasiv

266 Stable transformants were generated by overexpressing rat neuro

267 Sixteen transformants were generated by transforming the noninfe

268 Several plastid transformants were generated which contained either a Ni

269 ented by T-DNA integrations in studies where transformants were identified by selection.

270 Pools of 2,000 transformants were inoculated into the left and right mi

271 though the kinetics of sulfate uptake in the transformants were markedly altered with respect to tran

272 expressing the same construct, the resultant transformants were neither characterized by an increased

273 Stable transformants were obtained with pGFP::SW2 derivatives c

274 Maize transformants were produced via butafenacil selection us

275 Transformants were recovered with close to 10% efficienc

276 ility, growth, and development because fewer transformants were recovered, and most were dwarfed with

277 dures, the mutation rates of regenerants and transformants were relatively low, with no significant d

278 Interestingly, all vir-containing transformants were resistant to infection by MAV1.

279 Approximately 1000 stable transformants were screened individually for the product

280 ells via tungsten microparticles, and stable transformants were selected via a linked hygromycin B re

281 Finally, transformants were shown to adhere to rat L-2 lung cells

282 Biofilm-negative mutants of these transformants were shown to have a transposon insertion

283 The resulting transformants were similar to the corresponding wild-typ

284 The transformants were tested for antimicrobial susceptibili

285 Only erbB-2/neu transformants were tumorigenic when transplanted into is

286 TGR') occur with a frequency of 7-20% of all transformants when the minimum requirements for allele r

287 ) of chromosomal region capture during yeast transformants which in turn requires a time-consuming sc

288 rrelated with the decrease of starch in both transformants, which suggests that it is due to an alter

289 ially suppressed the defects of PRP27 -HopAI transformants, which were significantly reduced in Mps1

290 constructs has been directly quantified from transformant whole cell lysates.

291 The use of the transformant with its constitutive promoter was more int

292 C. muridarum transformants with an in-frame deletion of either pgp3 o

293 With the other strains, we could easily get transformants with extrachromosomal plasmid DNA when clo

294 Treatment of the Ddi1 transformants with HIV proteinase inhibitors showed diff

295 ecting or screening for transgenic activity, transformants with integrations into genomic regions tha

296 cribed delitto perfetto system for selecting transformants with integrative recombinant oligonucleoti

297 ion mutant phenotype was reproduced by using transformants with premature termination codon insertion

298 a pOp-GUS reporter, Lh214 and Lh314 yielded transformants with substantially lower GUS activities th

299 rect efficient facile screening of bacterial transformants with the goal of selecting, retrieving, an

300 However, transformants with the transcriptional fusion construct