ライフサイエンスコーパス: transgene expression

1 ition from 20 weeks and mice with continuous transgene expression.

2 TH antisera was used to corroborate targeted transgene expression.

3 ter intron loss caused a 4-fold reduction in transgene expression.

4 sociated chromatin locus to activate in situ transgene expression.

5 mized expression constructs and strains help transgene expression.

6 polyplex but contrastingly greater levels of transgene expression.

7 This, in turn, initiates calcium-dependent transgene expression.

8 named pH11, which enables stable and robust transgene expression.

9 onfirmed the enhancement of polymer-mediated transgene expression.

10 adult mice, despite optimal and long-lasting transgene expression.

11 on of cultured cells and supported transient transgene expression.

12 ce of versican respond with an activation of transgene expression.

13 examine mucosal lentiviral vector uptake and transgene expression.

14 h epigenetic variation and/or instability of transgene expression.

15 robust capacity for retrograde infection and transgene expression.

16 blasts into integration-free iCMs via robust transgene expression.

17 h activation of GFP expression indicates Cre transgene expression.

18 ation of novel epialleles, and regulation of transgene expression.

19 1BS element mutations resulted in variegated transgene expression.

20 e molecular mechanisms underlying successful transgene expression.

21 from methylation, thereby conferring stable transgene expression.

22 inhibition of signaling pathways or enforced transgene expression.

23 antial to generate higher and more prolonged transgene expression.

24 between cellular information and chromosomal transgene expression.

25 As reported, SW mice were very sensitive to transgene expression.

26 hese proteolytic sites in CD44 also inhibits transgene expression.

27 or the ability to drive heterologous nuclear transgene expression.

28 genesis to achieve ubiquitous and persistent transgene expression.

29 tional mutagenesis and positional effects on transgene expression.

30 lthy and exhibit a strong beta-cell-specific transgene expression.

31 tase inhibitors decrease adenoviral-mediated transgene expression.

32 response, reflected by strong and ubiquitous transgene expression.

33 an-mediated enhancement of adenoviral vector transgene expression.

34 tein isoforms, relying instead on cDNA-based transgene expression.

35 vels and reducing risks of heritable loss of transgene expression.

36 and 1 could also enhance vector-independent transgene expression.

37 ile effectively mediating gene silencing and transgene expression.

38 materialise in large part due to inefficient transgene expression.

39 crops used strong constitutive promoters for transgene expression.

40 rprisingly sensitive reporters of off-target transgene expression.

41 ne expression in addition to more persistent transgene expression.

42 plastid transformation vector for inducible transgene expression.

43 neurons and are capable of stable, long-term transgene expression.

44 inclusion of an intron was also required for transgene expression.

45 ansferase (UFGT), which was dependent of the transgene expression.

46 evious work demonstrated that SC-Ads amplify transgene expression 100-fold and produce markedly stron

47 , characterized by a robust induction of the transgene expression (17.7- to 73-fold) in the presence

48 y the most highly preferred codons decreased transgene expression (77- to 111-fold) when compared wit

49 ed from a colorectal cancer cell line showed transgene expression after i.v. CPS injection in an anim

50 aled significantly reduced levels of hepatic transgene expression after intravenous injection of dend

51 ted from preferential promoter usage because transgene expression after intravenous scAAV9/MeCP2-GFP

52 Irrespective of sex or transgene expression, ageing consistently reduced the se

53 Doxycycline administration can turn off the transgene expression allowing the restoration of Rai1 no

54 Stable transgene expression analyses, on multiple independent l

55 mutant algal strains that permit high-level transgene expression and by determining the contribution

56 INPACT system provides exquisite control of transgene expression and can be adapted to potentially t

57 have developed a system to achieve sustained transgene expression and ECM formation by hMSCs.

58 state in the liver consistent with sustained transgene expression and hepatic PAH enzyme activity wit

59 a T regulatory response that allows ongoing transgene expression and indicates that immunomodulatory

60 he phenotype can be reverted by interrupting transgene expression and is EGFR dependent, as gland siz

61 o be less degraded by proteasomes, preserved transgene expression and largely avoided hepatotoxicity.

62 We evaluated the stability of transgene expression and long-term safety in 10 patients

63 tigated the biomass, exogenous Bt toxins, Bt-transgene expression and methylation status in Bt rice e

64 ences (CDSs) with higher GC content improved transgene expression and resulted in a remarkable trans-

65 These mice show ubiquitous transgene expression and TDP-43 knockdown in both the pe

66 choice of transcriptional promoters to drive transgene expression and the best ways to sink existing

67 ansion, oncogenic transformation, variegated transgene expression and transcriptional silencing.

68 thylation patterns that may adversely affect transgene expression and vector stability in vivo.

69 ) are known to allow robust and reproducible transgene expression and were targeted for integration o

70 ng tumors in response to urethane, even when transgene expression (and therefore epithelial NF-kappaB

71 of integrated T-DNA molecules likely affect transgene expression, and control of integration is cons

72 aminitis, significant reduction in factor IX transgene expression, and loss of transduced hepatocytes

73 of undefined T-cell mixtures, variability of transgene expression, and terminal differentiation of ce

74 -DZ cells results in a similar activation of transgene expression, and treatment with dasatinib, an i

75 is, site-of-action experiments and exogenous transgene expression; and we provide a basic driver and

76 nce of nonintegrating vectors, and sustained transgene expression are among the major challenges in g

77 High levels of transgene expression are key to the success of improving

78 mutant algal strains that permit high-level transgene expression are less susceptible to epigenetic

79 which allows position-independent, long-term transgene expression, are particularly promising.

80 vivo and determined that treatment initiated transgene expression as early as 2 days posttransduction

81 gue T5660136 significantly reduced Ad5 liver transgene expression at 48 h post-intravenous administra

82 s identified as the optimum region to direct transgene expression at a higher level.

83 e therapy are inefficient due to short-lived transgene expression attributed to the cytosine-phosphat

84 icle-mediated transformation, result in poor transgene expression because of integration of truncated

85 Surprisingly, we found that turning off the transgene expression before the onset of the phenotypes

86 ninvasive imaging of MSC biodistribution and transgene expression before therapeutic radioiodine appl

87 tudy using an AAV2/8 vector in dogs reported transgene expression beyond the boundary of the subretin

88 ed a detailed study of the lateral spread of transgene expression beyond the subretinal injection sit

89 d AAV (ssAAV) vectors significantly augments transgene expression both in human cell lines in vitro a

90 Sinus rhythm animals had strong transgene expression but no atrial conduction changes.

91 ected target tumor cells in an RD manner for transgene expression but that could be "switched" into a

92 ) model of hemophilia A, we increased cFVIII transgene expression by at least 100-fold with the use o

93 Suppressing the transgene expression by doxycycline (Dox) treatment resu

94 at the cationic RC5K can induce even greater transgene expression by increasing payload capacity with

95 of hyaluronan in vitreous and CD44 modulates transgene expression by initiating CD44 proteolysis and

96 ngs led to greater than 10-fold increases in transgene expression by multiple target cell types when

97 transgene expression would improve long-term transgene expression by using an engineered piggyBac tra

98 wn that reliability of the A2UCOE in driving transgene expression can be attributed to its resistance

99 In this system, the transgene expression can be repressed in a doxycycline-d

100 cing via RNAe is irreversible, we found that transgene expression can be restored when hemizygous tra

101 Our results show that CAPN3 transgene expression can be successfully suppressed in t

102 Transgene expression, capillary density, graft function,

103 oses required to reach therapeutic levels of transgene expression caused liver inflammation in some p

104 d that uptake of FHBG (a PET radiotracer for transgene expression) coincided with expression of the f

105 frequency (PRF) for 2min exhibited superior transgene expression compared to all other treatment gro

106 circle DNA give enhanced and more persistent transgene expression compared to plasmid DNA in a number

107 We observed transgene expression confined to the renal cortex (speci

108 These results indicate that long term transgene expression consistency and T-DNA insert stabil

109 Long term transgene expression consistency and T-DNA insert stabil

110 s study addresses T-DNA insert stability and transgene expression consistency in multiple cycles of f

111 s study addresses T-DNA insert stability and transgene expression consistency in multiple cycles of f

112 The Tet-On/Off system for conditional transgene expression constitutes state-of-the-art techno

113 We found that repression of early transgene expression could prevent the priming of lucife

114 d RBPJ DNA binding ablates all Dll4 enhancer-transgene expression despite the presence of multiple fu

115 H-driven medulloblastoma, animals with Atoh1 transgene expression developed highly penetrant medullob

116 Moreover, the transgene expression dissipated after healing was comple

117 alpha-synuclein transgenic mouse model with transgene expression driven by the hindbrain-selective p

118 ome is able to persist and provide prolonged transgene expression during in vitro differentiation of

119 ion and localization, level, and duration of transgene expression, essential prerequisites for exact

120 AVrh32.33 vectors could establish persistent transgene expression, evade immunity, and minimize infil

121 However, transgene expression falls rapidly postdelivery due to a

122 uring the delivery phase with activation and transgene expression following arrival at the target sit

123 flanking sequences that can be used to drive transgene expression for any organism where transcriptom

124 ., PLK1) can significantly enhance non-viral transgene expression for applications in biotechnology a

125 rs were non-cytotoxic and provided sustained transgene expression for at least three weeks in vitro.

126 Site-specific gene addition can allow stable transgene expression for gene therapy.

127 hydrogel in vivo led to a sustained level of transgene expression for more than two months while mini

128 We detected persistent ABCA4 transgene expression for up to 8 months after injection

129 Suppression of the transgene expression from 18 to 24 months led to reversa

130 ry region as important elements for vigorous transgene expression from a vector that is functionally

131 ich is transcriptionally inactive, efficient transgene expression from AAV vectors is dependent upon

132 -repeat enhancers have proven safe; however, transgene expression from cellular promoters is often in

133 100 rounds of cell division showed sustained transgene expression from episomes and provided molecula

134 Suppression of therapeutic transgene expression from retroviral gene therapy vector

135 s is greatly hampered by the inefficiency of transgene expression from the nuclear genome.

136 .3- to 8-fold or 91- to 125-fold increase of transgene expression from the same batch of materials, d

137 and histone H3K4me3 patterns correlated with transgene expression; H3K9me3 marks partially correlated

138 We directed transgene expression in a fraction of HSCs that maintain

139 e versican G1 in enhancing adenoviral vector transgene expression in a hyaluronic acid-CD44 independe

140 This enhancer drives reporter transgene expression in a manner that recapitulates endo

141 mouse lungs resulted in a 6-fold increase in transgene expression in addition to more persistent tran

142 neurons, whereas scAAV9/beta-actin-GFP drove transgene expression in astrocytes.

143 ific delivery as well as providing sustained transgene expression in blood vessels.

144 ue-specific enhancers enables the control of transgene expression in both space and time.

145 nsgenic mouse lines that differ in levels of transgene expression in brain and periphery (APP23 mice,

146 The LV/CMV/ZGFP exhibited prolonged transgene expression in CHO cells and HSCs up to 10 days

147 0 kb of genomic 5'-flanking sequence, showed transgene expression in CNS oligodendrocytes from larval

148 ulated adeno-associated virus (AAV)-vectored transgene expression in cultured mammalian cells and mic

149 dorsal root crush and adeno-associated virus transgene expression in dorsal root ganglia.

150 how that myostatin inhibition by follistatin transgene expression in Dysf(-/-) mice results in early

151 neurons, whereas scAAV9/beta-actin-GFP drove transgene expression in GFAP(+) astrocytes.

152 Transgene expression in hPSCs is tightly regulated in re

153 Flcn H255Y mutant transgene expression in kidney-targeted Flcn knockout mi

154 causes selective up-regulation of exogenous transgene expression in mammalian cells.

155 a beta-galactosidase reporter gene abolished transgene expression in mice in vivo.

156 V2G9 chimera mediates rapid onset and higher transgene expression in mice.

157 13 deficiency, combined with conditional MYC transgene expression in mouse GC B cells, promotes lymph

158 igh-level stable gene transfer and sustained transgene expression in multiple primary human somatic c

159 ctive herpes simplex virus (HSV) vectors for transgene expression in nonneuronal cells in the absence

160 Efficient replication and transgene expression in normal human endothelial cells i

161 ted Nyx(nob) retina, robust and targeted Nyx transgene expression in ON BCs partially restored the ER

162 We demonstrate temporal manipulation of Gal4 transgene expression in only primary motoneurons and not

163 Megakaryocyte-specific transgene expression in patient-derived induced pluripot

164 as of the hepatic lobule, while there was no transgene expression in periportal areas.

165 ficiency germline transgenesis and sustained transgene expression in pigs by using the Sleeping Beaut

166 rovides both activation and amplification of transgene expression in planta.

167 ficiency germline transgenesis and sustained transgene expression in rabbits by using the Sleeping Be

168 it allows combinatorial cloning for multiple transgene expression in seeds, vegetative organs, or bot

169 tively and specifically drives Cre-dependent transgene expression in selected postsynaptic neuronal t

170 ivation of NMD cell autonomously knocks down transgene expression in specific cell types without affe

171 rged as a widely used platform for achieving transgene expression in specified neural populations.

172 d drove the highest level and specificity of transgene expression in stems.

173 Apart from the retina, we found transgene expression in subsets of periventricular neuro

174 ng in minimal cellular infiltrate and stable transgene expression in target muscles.

175 uronal-specific promoters were able to drive transgene expression in TG neurons.

176 e that lentiviral vector-mediated GT induces transgene expression in the B-cell compartment, resultin

177 ated lipoplexes that mediated high levels of transgene expression in the COS-7, HEK 293T/17, HeLa, an

178 simply genetically manipulated for efficient transgene expression in the differentiated cells, surmou

179 ossibility to drive efficient and persistent transgene expression in the heart offers the possibility

180 ic injection of different rat brain regions; transgene expression in the hippocampus lasted up to 6 m

181 ered CELiD DNA resulted in long-term, stable transgene expression in the liver.

182 ific T cells in vivo versus the stability of transgene expression in the muscle, we confirmed that th

183 and mediate localized, robust, and sustained transgene expression in the rat brain.

184 Transgene expression in these cells, however, can be sta

185 0 that is necessary and sufficient to direct transgene expression in this cardiac context.

186 enous Bacillus thuringiensis (Bt) toxins and transgene expression in transgenic rice under different

187 CPL4, the overexpression of which inhibited transgene expression in transient assays.

188 y retrotransposon can increase and stabilize transgene expression in transposon-mediated random inser

189 ose-dependent endothelial cell infection and transgene expression in tumor biopsies of diverse histol

190 ia the vessel, inertial cavitation increased transgene expression in tumour cells by up to 200 times.

191 ficiency germline transgenesis and sustained transgene expression in two important biomedical models,

192 d to achieve temporal and spatial control of transgene expression in various tissues in C. elegans.

193 d, tissue-specific and copy-number dependent transgene expression in vitro and in vivo.

194 viding cell population and provide sustained transgene expression in vitro and in vivo.

195 Subsequently, transgene expression in vitro in RAW 264.7 cells and ex

196 e inability to achieve sustained, high-level transgene expression in vivo presents a significant hurd

197 as associated with markedly higher levels of transgene expression in vivo, particularly within draini

198 Chlamydomonas RPL23 sequences also enabled transgene expression in Volvox carteri.

199 bled highly specific, long-term AAV-mediated transgene expression in white or brown adipocytes.

200 an inducible system to control the timing of transgene expression in zebrafish and chick.

201 n system for spatial and temporal control of transgene expression in zebrafish rod photoreceptors.

202 dated in functional rescue experiments using transgenes expression in EPCs from retroviral vectors.

203 efforts have been made in refining mosquito transgene expression, in particular attenuating the effe

204 otor phenotypes correlate with levels of FUS transgene expression, indicating that toxicity in develo

205 man neurodegenerative disease, whereas Tau35 transgene expression is equivalent to less than 10% of t

206 However, transgene expression is not amenable for therapeutic app

207 ply, while, to some extent, the exogenous Bt-transgene expression is reduced at sub-N levels (1/4 and

208 elerated degeneration induced by follistatin transgene expression is specific to mice lacking dysferl

209 r mRNA therapeutics when predictable in vivo transgene expression kinetics is imperative.

210 Transfection efficiencies and transgene expression kinetics of messenger RNA (mRNA), a

211 PsGA3ox1 transgene expression led to higher GA1 concentrations in

212 nts in a system in which genetic background, transgene expression levels and post-translational prote

213 , providing design strategies for increasing transgene expression levels and reducing risks of herita

214 At transgene expression levels comparable to endogenous CtB

215 Non-viral vectors often yield transgene expression levels lower than viral counterpart

216 By assessing the correlation of transgene expression levels with isoprenoid marker metab

217 iple promoters and terminators, and variable transgene expression levels.

218 We analyzed transgene expression (luciferase) and the local tissue d

219 Use of megakaryocytes/platelets for transgene expression may take advantage of their rapid t

220 ecal space surrounding the spinal cord, with transgene expression observed within multiple cell types

221 studies with cell type-specific postsynaptic transgene expression of designer receptors activated by

222 We show that transgene expression of Dm-dNK in Tk2 knock-out (Tk2(-/-

223 Ezh2 transgenic mouse model and showed that transgene expression of Ezh2 was sufficient to increase

224 n phenotype and the lethality are rescued by transgene expression of FATP4 in suprabasal keratinocyte

225 We therefore exploited the power of targeted transgene expression of mutant hDAT in Drosophila to exp

226 cellular promoters result in relatively low transgene expression, often leading to inadequate diseas

227 rough the BBB with FUS led to dose-dependent transgene expression only in the FUS-treated region that

228 editing technologies and tight regulation of transgene expression opens new doors for engineering.

229 hifts, allowing for cell-state and cell-type transgene expression optimization through codon content

230 In mice, fibroblast-specific Il11 transgene expression or Il-11 injection causes heart and

231 Re-expression of HEXIM1 (through transgene expression or localized delivery of a small mo

232 RGD loop had little to no effect on in vivo transgene expression or transgene-specific adaptive immu

233 establishing simple principles of successful transgene expression, our data open up new possibilities

234 PBAE-MPPs achieved robust transgene expression over at least 4 mo following a sing

235 ovides a useful molecular tool for directing transgene expression, particularly where up-regulation i

236 worked with transgenic sublines with drifted transgene expression patterns.

237 omoter sequences that can drive constitutive transgene expression patterns.

238 y types, including searching neurons against transgene expression patterns.

239 Transgene expression persisted for weeks after implantat

240 nd suggest simple strategies to overcome the transgene expression problem in Chlamydomonas.

241 cus at disease-relevant levels and display a transgene expression profile that recapitulates that of

242 conventional nanocomplexes, or DNA-CN), and transgene expression reached beyond the tumor edge, wher

243 tic biology, specifically in genome editing, transgene expression regulation, and bioenergy crop engi

244 The ability to induce transgene expression repeatedly via administration of an

245 Epigenetic silencing of transgene expression represents a major obstacle for the

246 eta-actin promoters to drive low versus high transgene expression, respectively.

247 Transgene expression restored GLP-1R-dependent stimulati

248 tion site-independent, copy number-dependent transgene expression restricted to macrophages, we demon

249 metry demonstrated that cardiolipin synthase transgene expression resulted in improved mitochondrial

250 Transgene expression resulted in increased proteolytic a

251 Moreover, cardiolipin synthase transgene expression results in alterations in signaling

252 g the postnatal period, cardiolipin synthase transgene expression results in marked changes in the te

253 We developed a method for transgene expression specifically in mouse endometrium a

254 genic mouse model with doxycycline-inducible transgene expression specifically in the glomerular podo

255 l tumors, a subset of which exhibited strong transgene expression, suggesting loss of Vhl function in

256 curling virus, drives levels of constitutive transgene expression that are significantly higher than

257 luding diet, oxygen availability, Huntingtin transgene expression, the absence of macroautophagy (ATG

258 Coupled with issues pertaining to transgene expression, the therapeutic landscape has unde

259 We wanted to determine the durability of transgene expression, the vector dose-response relations

260 , which was critical to achieving widespread transgene expression throughout orthotopic rat brain tum

261 (PBAE-MPPs) provided uniform and high-level transgene expression throughout the mouse lungs, superio

262 equires Cre-driver mouse lines that restrict transgene expression to a precisely defined cell populat

263 translation start site within exon 1 allowed transgene expression to be regulated by endogenous CD40L

264 bly in terms of both efficiency and reliable transgene expression to classic pronuclear microinjectio

265 This is the longest reported transgene expression to date from a parenterally adminis

266 pecific endogenous gene promoters to confine transgene expression to individual cell types (eg, ITGA2

267 ve previously shown that linking therapeutic transgene expression to induction of the chemokine CCL5/

268 binatorial specificity and stable, long-term transgene expression to isolate precisely defined neuron

269 ated sequences could drive stable luciferase transgene expression to significantly higher levels than

270 tory protein promoter, which largely limited transgene expression to the respiratory bronchioles.

271 cells, as well as cancer cells, resulting in transgene expression, vasculature disruption, and tumor

272 onic plasmid (MIP) is an alternative, robust transgene expression vector for reprogramming.

273 Up to a fiftyfold increase in tumor transgene expression was achieved, without any observabl

274 muscle samples revealed that increased GLUT4 transgene expression was associated with decreased level

275 we generated novel transgenic mice in which transgene expression was driven by an alpha-gustducin pr

276 Transgene expression was evaluated at four different gen

277 a vitreous component, in adenoviral-mediated transgene expression was examined.

278 Transgene expression was increased approximately 50-fold

279 hCD39 transgene expression was localized to the vascular endot

280 on by food intake), recombinant AAV-mediated transgene expression was markedly reduced, both in vitro

281 However, transgene expression was poor, most likely due to endoso

282 xpression and abated over several weeks once transgene expression was suppressed.

283 Zap70 transgene expression was then ablated by withdrawal of d

284 se, which was associated with a reduction in transgene expression, was characterized more thoroughly

285 After 1 and 3 months of transgene expression, we found robust increases in tau p

286 In order to achieve kidney-specific transgene expression, we tested direct hydrodynamic inje

287 sive stages of the mdx pathology achieved by transgene expression were paralleled by reduced expressi

288 nucleus and the SC are lost after 8 weeks of transgene expression, whereas those in the oculomotor, t

289 cation, or TRAP, combines cell type-specific transgene expression with affinity purification of trans

290 bly in terms of both efficiency and reliable transgene expression with classic pronuclear microinject

291 bly in terms of both efficiency and reliable transgene expression with classic pronuclear microinject

292 now examine the consequences of suppressing transgene expression with doxycycline on the NFT-associa

293 dent on tau expression, since a reduction of transgene expression with doxycycline reversed the MT ch

294 ed components, remote stimulation of insulin transgene expression with RF or a magnet lowers blood gl

295 reflect preferential promoter usage because transgene expression with scAAV9/MeCP2-green fluorescent

296 Transgene expression with the PBAE/PBAE-PEG blended nano

297 ed inflammatory response and more persistent transgene expression, with luciferase activity persistin

298 The ability to control transgene expression within specific tissues is an impor

299 enes have been co-opted to produce selective transgene expression within this population.

300 We hypothesized that delaying initial transgene expression would improve long-term transgene e