ライフサイエンスコーパス: transglycosylation

1 ucan oligosaccharides was not accompanied by transglycosylation.

2 c acceptor toward some endoglycosynthases in transglycosylation.

3 y but are able to take glycan oxazolines for transglycosylation.

4 o enzymatic activities, transpeptidation and transglycosylation.

5 t favored nonfucosylated GlcNAc acceptor for transglycosylation.

6 implying the presence of enzymes that favour transglycosylation.

7 the enzymatic synthesis of disaccharides by transglycosylation.

8 N322Q was much more efficient than N322A for transglycosylation.

9 to act as critical catalytic residues in the transglycosylation.

10 ers using a tandem endoglycosidase-catalyzed transglycosylation.

11 duct as well as to its enhanced activity for transglycosylation.

12 e the high-mannose-type glycan oxazoline for transglycosylation.

13 structure in the resulting glycoprotein upon transglycosylation.

14 synthesis in S. aureus by interference with transglycosylation.

15 ting molecular grafting during integrational transglycosylation.

16 ylation whereas they inhibited restructuring transglycosylation.

17 the lac operon, is the preferred product of transglycosylation.

18 ctive in general than the Endo-S mutants for transglycosylation.

19 alytic mechanism, substrate specificity, and transglycosylation acceptor specificity of guinea pig li

20 e resulting mutants for their hydrolysis and transglycosylation activities.

21 These mutants possess the transglycosylation activity but lack the hydrolytic acti

22 ites -2, -3 and +2 and showed an increase in transglycosylation activity compared with the wild type.

23 that lacks hydrolytic activity but possesses transglycosylation activity for glycoengineering.

24 These data indicate that the transglycosylation activity of CBG derives from the form

25 report here our study on the hydrolytic and transglycosylation activity of recombinant Endo-D and it

26 g the 2-position could have an impact on the transglycosylation activity of several GHs.

27 The transglycosylation activity of the endo-beta-N-acetylglu

28 lycoproteins was described that explores the transglycosylation activity of the endo-beta-N-acetylglu

29 This finding, coupled with the remarkable transglycosylation activity of the EndoS glycosynthase m

30 different properties, some possessed potent transglycosylation activity with diminished hydrolysis a

31 glycosynthases that demonstrated remarkable transglycosylation activity with only marginal or no pro

32 Endo-D also possesses transglycosylation activity with sugar oxazoline as the

33 ci holds that peptidoglycan synthesis (i.e., transglycosylation and cross-linking) is restricted spat

34 ntrolling the balance between hydrolysis and transglycosylation and driving the elongation of beta(1-

35 at residues implicated in the enhancement of transglycosylation and synthetic capacity are proximal t

36 a broad substrate specificity of Endo-A for transglycosylation, and the chemoenzymatic method descri

37 ndoglycosidase-catalyzed deglycosylation and transglycosylation approach is emerging as a promising p

38 ylation, using the endoglycosidase-catalyzed transglycosylation as the key reaction.

39 The RNA-TAG (transglycosylation at guanosine) is carried out by a bac

40 and vancomycin are comparable inhibitors of transglycosylation, but that oritavancin is a more poten

41 To elucidate the molecular basis of transglycosylation by MalQ, we have determined three cry

42 uated as substrates for the Endo-A-catalyzed transglycosylation by use of ribonuclease B as a model s

43 ue that both integrational and restructuring transglycosylation can contribute to both wall-assembly

44 specificities, active site organization, and transglycosylation capacity.

45 terminus, was able to serve as acceptors for transglycosylation catalyzed by Endo-A and EndoM-N175A.

46 tadynamics simulations, that it is tuned for transglycosylation (DeltaG() = 12 kcal/mol).

47 It catalyzes transglycosylation/disproportionation reactions in which

48 everal mutants showed significantly enhanced transglycosylation efficiency over the wild type enzyme.

49 sn or alpha1,6-fucosyl-GlcNAc-polypeptide in transglycosylation, enabling a highly convergent synthes

50 of TDP-desosamine was also demonstrated in a transglycosylation experiment.

51 The importance of transglycosylation for cell wall assembly is thus firmly

52 apshots" of substrate binding and hydrolysis/transglycosylation giving the first insights into the me

53 of knowledge about the molecular details of transglycosylation hampers the rational design of TGs.

54 We measured remarkably high transglycosylation:hydrolysis ratios under optimized con

55 sm of action of oritavancin is inhibition of transglycosylation (important in peptidoglycan synthesis

56 t control the balance between hydrolysis and transglycosylation in these enzymes are not understood.

57 irst experimental evidence for restructuring transglycosylation in vivo.

58 ntennary glycan oxazolines as substrates for transglycosylation, in contrast to previously reported e

59 complex N-glycan oxazoline as substrate for transglycosylation, indicating their strict substrate sp

60 s at least 3 known mechanisms: inhibition of transglycosylation, inhibition of transpeptidation, and

61 rence in vivo of two types of interpolymeric transglycosylation: "integrational" (in which a newly se

62 Its capacity for transglycosylation is a factor in this activation.

63 of peptidoglycan synthesis via inhibition of transglycosylation is common to all glycopeptides (vanco

64 emarkable in predominantly catalysing hetero-transglycosylation: its preferred donor substrates (cell

65 This slow transglycosylation may provide a mechanism in vivo for f

66 carbose-derived decasaccharide by a two-step transglycosylation mechanism.

67 to-oligosaccharides except maltose for which transglycosylation nonetheless dominated across a range

68 This confirms that restructuring transglycosylation occurred between pairs of previously

69 1-->4) > (1-->2) > (1-->6)] with significant transglycosylation occurring in the early stages of the

70 With time, transglycosylation occurs, generating (GlcNAc)8 from the

71 Transglycosylation of galactomannan from the membrane to

72 the present study, a novel in situ enzymatic transglycosylation of stevioside has been developed by p

73 The results confirm that the transglycosylation of stevioside led to an enrichment in

74 are enzymes that catalyze the hydrolysis and transglycosylation of xyloglucan polymers in plant cell

75 r three proteins are capable of carrying out transglycosylation of xyloglucans.

76 cells, either via direct re-incorporation by transglycosylation or via internalization and metabolic

77 The enzyme catalyzes a transglycosylation reaction in which guanine is eliminat

78 EC 2.4.2.29) catalyzes a posttranscriptional transglycosylation reaction involved in the incorporatio

79 -981 and Asn-1029 significantly affected the transglycosylation reaction, indicating their essential

80 The alpha1,2FT catalyzes a transglycosylation reaction, resulting in syntheses of t

81 ase for the 4-OH of GlcNAc to facilitate the transglycosylation reaction.

82 , forming new polymers through non-enzymatic transglycosylation reactions (TGRs).

83 rmediate and that the specificity of CBG for transglycosylation reactions is different from its speci

84 Kinetic studies revealed that correction for transglycosylation reactions is necessary to derive corr

85 provided evidence that transpeptidation and transglycosylation reactions occur in pathogenic Chlamyd

86 density increase suggests that restructuring transglycosylation reactions occurred between the now wa

87 g peptidoglycan via the transpeptidation and transglycosylation reactions of cell wall synthesis, gen

88 CBG also catalyzes a variety of transglycosylation reactions, which have been been shown

89 yuridine via acid catalyzed anomerization or transglycosylation reactions.

90 sugar chains through glycosynthase-catalyzed transglycosylation reactions.

91 binding through hydrolysis, condensation or transglycosylation reactions.

92 ly (11 and 16 kcal/mol for glycosylation and transglycosylation, respectively) and alters the conform

93 ctly with bacterial proteins involved in the transglycosylation step of cell wall biosynthesis.

94 The transglycosylation step of cell wall synthesis is a prim

95 ed a set of small molecules that inhibit the transglycosylation step of peptidoglycan synthesis to di

96 We describe the improvement of transglycosylation (TG) by chitinase D from Serratia pro

97 sidues and a novel endoglycosidase-catalyzed transglycosylation that simultaneously added two N-glyca

98 During the process of transglycosylation, the quencher-appended polyprenol is

99 hydrolysis was also accompanied by extensive transglycosylation to give transitory accumulations of h

100 isotope internal standard was synthesized by transglycosylation using a recombinant alpha-amylase.

101 still able to catalyze N-glycan synthesis by transglycosylation using activated oxazoline donors.

102 IgG1-Fc through an endoglycosidase-catalyzed transglycosylation, using sugar oxazolines as the donor

103 It was found that the enzymatic transglycosylation was efficient with native GlcNAc-cont

104 To detect transglycosylation, we shifted heavy rose cells into lig

105 some of the glycosyl linkages formed during transglycosylation were (1-->6)-beta.

106 rates for XETs) did not affect integrational transglycosylation whereas they inhibited restructuring

107 ar oxazoline as the donor substrate, but the transglycosylation yield is low due to enzymatic hydroly