ライフサイエンスコーパス: transient expression

1 nd its expression vector was constructed for transient expression.

2 a benthamiana using a geminiviral vector for transient expression.

3 t also localized to nuclei during protoplast transient expression.

4 between the two complexes were detectable in transient expression.

5 imply mixed with cells to reprogram them via transient expression.

6 l pathogens with cell death phenomenon under transient expression.

7 Agrobacterium-mediated transient expression analysis of N-terminally truncated

8 Using a plant protoplast transient expression analysis we have further revealed t

9 entire length of Aichi virus 3A followed by transient expression and affinity purification revealed

10 runcated SNARE constructs and antibodies for transient expression and analysis.

11 d-oleosin genes in Physcomitrella patens for transient expression and tobacco (Nicotiana tabacum) BY2

12 The results from transient-expression and/or infection assays indicated t

13 A transient expression assay suggested that pp71 and IE1 w

14 ir endogenous promoter in Arabidopsis and in transient expression assay.

15 antage of Agrobacterium tumefaciens-mediated transient expression assays (agroinfiltration) and virus

16 ion 118 partially maintains functionality in transient expression assays and supports efficient growt

17 ht-absorbing region of ZTL (the LOV domain), transient expression assays and ztl mutants to establish

18 Transient expression assays displayed CAMTA3 induction o

19 bidopsis promoters were cloned and tested in transient expression assays in Arabidopsis and lettuce p

20 performed Agrobacterium tumefaciens-mediated transient expression assays in Nicotiana benthamiana to

21 Using transient expression assays in Nicotiana benthamiana, we

22 ysis of the CC and LRR domains of RPS5 using transient expression assays in Nicotiana benthamiana.

23 In transient expression assays in tobacco (Nicotiana tabacu

24 Rice embryo transient expression assays indicated that both MYBS1 an

25 mportant because Glc signaling in protoplast transient expression assays is compromised by disruption

26 brane localization of OsCPK4 was observed by transient expression assays of green fluorescent protein

27 l role of SUMO1 and SUMO3 in T3 induction in transient expression assays was closely matched to the p

28 Transient expression assays were used to identify the ex

29 ane domain from SGRL reduced its activity in transient expression assays, although variants with and

30 with PRK4 and PRK5 in Nicotiana benthamiana transient expression assays, and associated at discreet

31 In transient expression assays, HvVP1 controls the activati

32 Using transient expression assays, we show that SNE1 suppresse

33 we examined their synthesis from plasmids in transient expression assays.

34 We observed experimental variability in transient-expression assays and developed a mathematical

35 Transient-expression assays revealed the colocalization

36 ial chromatin immunoprecipitation assays and transient-expression assays suggested that BCL6 recruitm

37 mutant proteins, using genetic analysis and transient-expression assays.

38 in ts8-22-infected cells and with pU(L)28 in transient-expression assays.

39 to mTORC1 in vitro parallels that seen with transient expression, but binding to mTORC1 in vitro is

40 to facilitate gB-mediated membrane fusion in transient-expression cell-cell fusion experiments.

41 Transient expression, ChIP, and co-IP studies show that

42 identity in stamen-derived petals, as their transient expression coincides with early expression of

43 the pEAQ vectors have mostly been used in a transient expression context, through agroinfiltration o

44 ientation were evaluated in rice plants with transient expression employing quantitative RT-PCR analy

45 cortex contrasts with findings by others of transient expression exclusively in axon tracts in the d

46 her during early development or by inducible transient expression exhibited extensive HA degradation,

47 Transient expression experiments demonstrated that OsGZF

48 Transient expression experiments demonstrated the capaci

49 In transient expression experiments with Sf9 cells, chimeri

50 34 physically interacted with HIF-1alpham in transient expression experiments.

51 regulation by viral and cellular proteins in transient-expression experiments and by mutagenesis of t

52 As predicted, their regulated transient expression greatly improved the differentiatio

53 f OsWRKY24 via particle bombardment-mediated transient expression in aleurone cells represses the exp

54 their capacity to regulate ABA signaling by transient expression in Arabidopsis protoplasts.

55 species present in the supernatant following transient expression in Chinese hamster ovary cells were

56 On transient expression in HEK293T cells, these mTOR mutant

57 Transient expression in HeLa cells of two different muta

58 he variants on promoter activity assessed by transient expression in HepG2 cells.

59 tion of new monoterpene synthase genes using transient expression in N. benthamiana and the improveme

60 Transient expression in Nicotiana benthamiana (tobacco)

61 (GGPP) diversities, and plant morphology by transient expression in Nicotiana benthamiana and overex

62 Transient expression in Nicotiana benthamiana as well as

63 Transient expression in Nicotiana benthamiana confirmed

64 Of the class II diTPSs, transient expression in Nicotiana benthamiana identified

65 Using transient expression in Nicotiana benthamiana leaf, we d

66 d to an IgG heavy chain fragment (EPO-Fc) by transient expression in Nicotiana benthamiana plants.

67 d genes together with Agrobacterium-mediated transient expression in Nicotiana benthamiana provided s

68 We confirm, using transient expression in Nicotiana benthamiana, that miR4

69 Upon transient expression in Nicotiana benthamiana, the TPS e

70 Localization of PRX17 was examined by transient expression in Nicotiana benthamiana.

71 the recombinant enzymes were obtained after transient expression in Nicotiana benthamiana.

72 noyl oxide into forskolin as demonstrated by transient expression in Nicotiana benthamiana.

73 NATA1 transient expression in Nicotiana tabacum and the additi

74 Transient expression in onion epidermal cells, transform

75 By transient expression in onion epidermis, we demonstrate

76 deling, in vivo stable isotope labeling, and transient expression in petunia flowers reveal that BALD

77 Following transient expression in planta, HopQ1 was shown to copur

78 ssion in tumor-derived cell lines as well as transient expression in primary glioblastoma cells.

79 haracterization of genetic plant parts using transient expression in protoplasts and dual luciferase

80 expression throughout development, although transient expression in some neuronal subpopulations was

81 g primarily transcribed in the vector with a transient expression in the host only at tick-bite sites

82 of these regulators was further confirmed by transient expression in tobacco (Nicotiana benthamiana)

83 lized to the surface of lipid droplets after transient expression in tobacco (Nicotiana tabacum) cell

84 reen fluorescent protein) in Arabidopsis and transient expression in tobacco (Nicotiana tabacum) indi

85 timeric and CLV1-BAM multimeric complexes in transient expression in tobacco and in Arabidopsis meris

86 s individually and in groups and showed, via transient expression in tobacco cells that nucleolar loc

87 Transient expression in tobacco epidermis of a truncated

88 pecificities and validated their function by transient expression in tobacco leaves and in vitro DNA

89 Its transient expression in vivo induced the expression of k

90 in parallel with CLV1, recent studies using transient expression indicated that CLV2 and CRN from a

91 Transient expression is a rapid, useful approach for pro

92 FA complex plants dramatically inhibited the transient expression level of T-DNA and only reduced T-D

93 anscriptional positive feedback can modulate transient expression lifetime to a greater extent than p

94 uction increases the risk of tumorigenicity, transient expression methods have considerably lower rep

95 ombination of stably transformed plants with transient expression modules.

96 Transient expression of 2a NS4B resulted in a pronounced

97 Transient expression of a carboxyl-terminal reporter gen

98 Indeed, we observed that transient expression of a constitutive membrane-bound mu

99 Transient expression of a GFP-fusion in tobacco demonstr

100 Based on transient expression of a luciferase reporter plasmid, t

101 Using transient expression of a panel of deletion and chimeric

102 Transient expression of a peptide composed of the amino-

103 Transient expression of a Pseudomonas aeruginosa Glc(NAc

104 Transient expression of a SlCAT9-YFP fusion in tobacco c

105 Transient expression of a ZmALMT1::GFP chimera confirmed

106 Transient expression of A20 reduced K63-linked ubiquitin

107 omain constructs are produced in parallel by transient expression of adherent human embryonic kidney

108 erentiating IBMX-then-dex treatment produced transient expression of adipogenic transcriptional regul

109 Transient expression of alpha4beta2 nicotinic receptors

110 t was localized in the apoplast, as shown by transient expression of an OsORAP1/green fluorescent pro

111 Here we show that stable or transient expression of ArPIKfyve(WT) in mammalian cells

112 hod to rapidly determine gene function using transient expression of artificial microRNAs (amiRNAs) i

113 During hematopoietic cell differentiation, transient expression of ASB2alpha, the specificity subun

114 Transient expression of betatrophin in mouse liver signi

115 This was reproduced by transient expression of both EGFP and other fluorescent

116 inside Fraction C' (large pre-BII) marked by transient expression of c-Myc.

117 Thus, transient expression of CAErbB2 in skeletal muscles lead

118 As expected, transient expression of CaMKIIalpha K42M in the NAcc she

119 Transient expression of Cav3, but not P104L, in HEK 293

120 Transient expression of CCR5 ZFNs permanently and specif

121 recursor cells (HSPC) can be modified by the transient expression of CCR5-targeted zinc finger nuclea

122 During T cell development and activation, transient expression of CD44 splicing variant 6 (CD44v6)

123 ver, these pathways alone cannot explain the transient expression of CDX1 in intestinal metaplasia or

124 totic downstream effector of p53 whereas the transient expression of CerS6 elevated PUMA in a p53-dep

125 olecular events occurring between the rapid, transient expression of cFOS and the subsequent MMP13 in

126 egrin-dependent cell migration similarly and transient expression of constitutively active Rac1 resto

127 Transient expression of CPRabA5e:EGFP fusion protein in

128 Finally, the vector is excised by transient expression of Cre recombinase (coexpressed wit

129 Transient expression of CycT1 in undifferentiated monocy

130 Transient expression of DeltaNp63-EGFP alpha and beta is

131 Transient expression of diphtheria toxin in beta cells o

132 s protocol yields efficient and reproducible transient expression of diverse genes and is exemplified

133 Transient expression of dominant-negative CHK1 raised ba

134 Transient expression of dominant-negative DeltaCLOCK blo

135 o rattle virus-derived plasmid for in planta transient expression of double stranded RNA (dsRNA) homo

136 Similarly, transient expression of E1B55K in IMR90 fibroblasts and

137 Transient expression of E2f3 in both HEK293 cells and in

138 The most striking finding was the transient expression of EAAT2 in layer V pyramidal neuro

139 We conclude that transient expression of EAAT2 occurs during the window o

140 ndent release of TNF-alpha induced rapid and transient expression of early growth response protein 1

141 Transient expression of EBF3 mutant proteins in HEK293T

142 Remarkably, transient expression of either microRNA induces the epig

143 Transient expression of either VCP/p97 or PP2Ac was suff

144 Interestingly, transient expression of EphA2 and/or Braf(V600E) in non-

145 Transient expression of ERdj4 and ERdj5 in X-box binding

146 h a localized proliferative response and the transient expression of established digit blastema marke

147 The transient expression of ETS-2 and PU.1 cDNAs in mesothel

148 Transient expression of FBP1 in FBP1 knockdown cells ful

149 Transient expression of FOXC1 in nontransformed mammary

150 s encoding alternative TMAO reductases; (ii) transient expression of frmRAB, encoding formaldehyde de

151 Photoperiod-induced transient expression of FT (FLOWERING LOCUS T) caused pr

152 Transient expression of full-length PB1-F2, truncated (2

153 Here, we show that transient expression of Gata3, Eomes, and Tfap2c in mous

154 Transient expression of GDP- and GTP-bound Rab8 mutants

155 of inoculation into fresh LB medium with the transient expression of genes involved in phosphate upta

156 Likewise, transient expression of Gli1, a downstream target of Shh

157 Furthermore, both nematode infection and transient expression of Gr-VAP1 in tomato plants harbori

158 Using transient expression of green fluorescent protein fusion

159 Transient expression of hCH25H in human hepatoma cells r

160 Here, we show that transient expression of HCV RNA-dependent RNA polymerase

161 Here, we identify the transient expression of histone demethylase LSD1 and the

162 with HLA-DR surface expression, Ab blocking, transient expression of HLA-DR without CIITA, and short

163 Epifluorescence imaging showed that transient expression of human phospholipase C-delta1, re

164 Finally, transient expression of ICP22 was sufficient to compleme

165 Transient expression of ICP27 and UL47 in HeLa cells sug

166 Myc-induced transient expression of ING4 is required for the differe

167 In contrast, transient expression of IRF4 decreases the SHIP1 level i

168 we have tested a mRNA-based method to induce transient expression of ITGA4, an adhesion molecule acti

169 and subclinical tubular injury, reflected by transient expression of Kim1 and vimentin in scattered p

170 -activated cell sorting we further show that transient expression of kindlin-1 or -2 in Chinese hamst

171 of proliferation rates that are restored by transient expression of miR-155.

172 Also, transient expression of miR-34a in glioblastoma cells st

173 later, at the common ILC precursor stage, by transient expression of mixed ILC1, ILC2 and ILC3 transc

174 hermore, treatment with recombinant MMP-9 or transient expression of MMP-9 is sufficient to reduce E-

175 Transient expression of mutant huntingtin in mouse prima

176 interaction between RPS15 and MDM2/MDMX and transient expression of mutant RPS15 revealed defective

177 ransgene on proliferation days 3 to 5 and by transient expression of Myc (alias c-myc).

178 Functionally, the transient expression of Na(+) and I(h) currents initiate

179 Rat H9C2 cardiomyoblasts with transient expression of nebulette underwent cyclic mecha

180 Due to the transient expression of Neurog1 and extensive migration

181 Transient expression of Neurog1 promoted widespread neur

182 Because transient expression of Neurogenin3 (Neurog3) in progeni

183 In planta transient expression of NnCYP76B6 showed a significant e

184 We suggest that the transient expression of Notch3 early in differentiation

185 Transient expression of NS1 depolarized electrically iso

186 at this stage appears to be mediated by the transient expression of Numb in the hemangioblast-derive

187 Transient expression of P104L caused a two-fold increase

188 levated in several cell lines in response to transient expression of p53 or in response to folate str

189 A previous study showed that the transient expression of pepper Bs2 in lemon leaves reduc

190 Consistent with a low and/or transient expression of PfMSP8 on the surface of merozoi

191 gments immediately after pruning triggered a transient expression of phenylalanine ammonia-lyase (PAL

192 Virus-induced gene silencing (VIGS) and transient expression of Phytophthora infestans effectors

193 Transient expression of PMEPA1 down-regulates AR protein

194 the portal-scaffold interaction seen during transient expression of portal and scaffold proteins.

195 Fate mapping studies following transient expression of PRAS40 demonstrated that mTORC1(

196 Transient expression of protein-green fluorescent protei

197 fection compared to the wild-type virus, and transient expression of pUL29/28 activated the major imm

198 We found that transient expression of Rab26 mutants and siRNA-mediated

199 Notably, transient expression of reprogramming factors in vivo re

200 a-amanitin, or the dissolution of R loops by transient expression of RNase H1, leads to the loss of s

201 no role in adulthood secondary to the short transient expression of SDF-1 and the delayed expression

202 Here, we use geminivirus-based replicons for transient expression of sequence-specific nucleases (zin

203 Transient expression of seven TPSs from A. thaliana, Cap

204 Transient expression of SGRL in leaves of Nicotiana bent

205 Similarly, transient expression of siRNAs into the primary effusion

206 We show that transient expression of six transcription factors Run1t1

207 Finally, transient expression of SlCipk6 in transgenic N benthami

208 Transient expression of Snapin not only rescues the slow

209 Interestingly, transient expression of SPFMV gene products coagroinfilt

210 course of the transformation, reveal that a transient expression of stem cell markers precedes the m

211 hermore, similar to the known requirement of transient expression of survivin for pathophysiological

212 not high-frequency tetani, produced a rapid, transient expression of synaptic CP-AMPARs that facilita

213 essential for functional genomics; however, transient expression of target proteins in maize cells i

214 Transient expression of tensins increases beta1-integrin

215 Here, we show that transient expression of Tfap2c, Gata3, Eomes, and Ets2 i

216 hat proper centripetal FC migration requires transient expression of the C/EBP encoded by slow border

217 of cell-associated NSM proteins derived from transient expression of the complete CCHFV M segment and

218 The transient expression of the corresponding cDNAs in straw

219 stably integrated gene construction, or the transient expression of the desired gene following the i

220 Transient expression of the entire pathway in Nicotiana

221 By transient expression of the ETS transcription factor ETV

222 Transient expression of the F and G (attachment) glycopr

223 Transient expression of the four essential entry glycopr

224 Neurogenesis is initiated by the transient expression of the highly conserved proneural p

225 We investigated the possibilities of transient expression of the immunosuppressive cytokine t

226 entiation-promoting cytokines in vitro or by transient expression of the myeloid transcription factor

227 elves have unique characteristics, including transient expression of the neuronal isoform of class II

228 trolled despite global hypomethylation, with transient expression of the pluripotency network, sugges

229 undetectable in cancer cell lines while the transient expression of the protein in cancer cells indu

230 ne responses were restored in these cells by transient expression of the STING protein but not after

231 ate lymphoid cell precursor characterized by transient expression of the transcription factor PLZF, a

232 Here, we show that transient expression of the transcription factors Ascl1

233 haematopoietic stem cells (rEC-HSCs) through transient expression of the transcription-factor-encodin

234 tor neurons showed axonal degeneration after transient expression of the TTBK1 gene or treatment with

235 Transient expression of the viral tegument proteins of p

236 Upon transient expression of these nucleases the target gene

237 Furthermore, the in planta transient expression of these three selected nanobodies

238 sive to HCV entry, we previously showed that transient expression of these two human genes is suffici

239 Due to the very early and transient expression of this gene, dissections were perf

240 P13 rats, the appearance, disappearance, and transient expression of three cortical events and oscill

241 Transient expression of three mutations made in residues

242 Moreover, transient expression of TIG3 leads to terminal different

243 This effect was rescued by transient expression of TPC2-GFP.

244 Thus, low and transient expression of transgenic IL-12 in hepatocytes

245 y, genes specifically induced in response to transient expression of two disease-associated mutations

246 These events are associated with the transient expression of two previously unidentified C-te

247 Biolistic transient expression of UBA2-GFPs demonstrated that the

248 Transient expression of UNC-43/CaMKII in adults rescued

249 Nicotiana benthamiana as a plant factory, by transient expression of up to four genes coding for cons

250 Transient expression of various gene constructs transfor

251 ficient lentiviral vectors may be useful for transient expression of various other genes-of-interest.

252 We show that the transient expression of VGLUT3 by a discrete population

253 The vast number and transient expression of virulence factors in the infecti

254 cell-rounding phenotype associated with the transient expression of wild-type and mutagenized forms

255 Transient expression of wild-type gigaxonin in GAN fibro

256 Transient expression of WRI1 with OLEOSIN1 in Nicotiana

257 ast, unlike in N. benthamiana and C. quinoa, transient expression of wt CP in nonhost N. clevelandii

258 Transient expression of wtEphB2, but not noncleavable Ep

259 Subsequent (and typically transient) expression of proneural factors promotes cell

260 he seven chimeric RNAs that follow the exact transient expression pattern as PAX3-FOXO1 are specific

261 For additional small-scale transient expression, purification and binding analysis,

262 icity, yet the functional importance of this transient expression remains unknown.

263 analysis of all pairwise interactions during transient expression revealed a robust interaction betwe

264 ation of unnatural amino acids is limited to transient expression settings that lead to very heteroge

265 Using a transient expression splicing assay, we find that 7SK sn

266 nza A virus infection by using silencing and transient expression strategies in human lung epithelial

267 Transient expression studies of MAB1 in tobacco (Nicotia

268 ifs and translocates to the plant nucleus in transient expression studies.

269 n shown to have strong antiviral activity in transient-expression studies, and together with A3G, it

270 f these were functionally characterized in a transient expression system consisting of Agrobacterium-

271 Collectively, this transient expression system facilitates tests of the phy

272 In this paper, we describe a transient expression system in Arabidopsis mesophyll pro

273 We established a transient expression system in Nicotiana benthamiana to

274 loned, and then expressed using the magnICON transient expression system in plants and their enzymati

275 Our cell-based transient expression system is fast and easy to set up a

276 Using the transient expression system of tobacco (Nicotiana tabacu

277 of chimeras between Rpg1b and Rpg1r using a transient expression system revealed that AvrB versus Av

278 V-1) to suppress silencing was examined in a transient expression system that employed an imperfect h

279 Isolated protoplasts serve as a transient expression system that is highly representativ

280 elements within the 180-bp NIA1 fragment, a transient expression system using agroinfiltration of Ni

281 This transient expression system was successfully applied to

282 t strain and a plant (Nicotiana benthamiana) transient expression system were used to test the abilit

283 AtPTB2 was analysed in an in vivo protoplast transient expression system with a novel mini-exon splic

284 Using a transient expression system, 20 additional mutant V prot

285 f mutant and wild-type ICP22, we developed a transient expression system.

286 produce alpha-onocerin in the N. benthamiana transient expression system.

287 om oncogenic K-ras through Rac1 was shown in transient expression systems and reporter assays.

288 We used two transient expression systems to investigate the molecula

289 Transient expression systems were used to characterize Z

290 made in defining trafficking in plants using transient expression systems.

291 racted with DeltaNp63alpha under stable- and transient-expression systems.

292 In common use are a number of strategies for transient expression that greatly reduce the preparation

293 hippocampal neurons are limited by toxicity, transient expression, the use of immature neurons and/or

294 protein levels, a departure from its normal transient expression upon the initiation of cell growth.

295 in suppressing IFN-beta expression, we used transient expression vectors to test the abilities of a

296 Additionally, barx1 transient expression was observed in the posterior later

297 Agrobacterium-mediated transient expression was used to screen putative Z. trit

298 Remarkably, by transient expression we show that also the Ave1 homologs

299 Moreover, using Agrobacterium-mediated transient expression, we found that overexpression of SI

300 dopsis and validated our method by comparing transient expression with expression in stably transform