1 on, and promoter activities were analyzed by
transient transfection.
2 (NFAT) were measured by reporter assay after
transient transfection.
3 C terminus and were expressed separately by
transient transfection.
4 d upregulated a number of these promoters in
transient transfection.
5 lear relocalization of PKR-EGFP expressed by
transient transfection.
6 pe, we induced GILZ expression in HUVECs via
transient transfection.
7 e products were examined by Western blot and
transient transfection.
8 ins less than 0.1 when NCX1 is expressed via
transient transfections.
9 ial methylation with Sss1 methylase prior to
transient transfections.
10 by chromatin immunoprecipitation (ChIP) and
transient transfections.
11 or both mutant receptors in COS-7 cells upon
transient transfection,
albeit at lower levels for T175A
12 Reactivation of FOXO1A by stable or
transient transfection also restored the growth-inhibito
13 By promoter deletion, mutation, and
transient transfection analyses, we have shown that this
14 scriptional activation of a reporter gene in
transient transfection analyses.
15 Transient transfection analysis demonstrated that PU.1 f
16 Transient transfection analysis showed that the TNF-alph
17 evaluation of transcriptional elements using
transient transfection analysis.
18 Transient transfection and chromatin immunoprecipitation
19 Transient transfection and chromatin immunoprecipitation
20 Transient transfection and electrophoretic mobility shif
21 Our previous studies, using
transient transfection and EMSA experiments, implicated
22 his protocol, we show how to use small-scale
transient transfection and fluorescence-detection size-e
23 In this study, using
transient transfection and immuno-precipitation assays,
24 Transient transfection and inducible expression experime
25 -beta response elements and a combination of
transient transfection and knock down assays revealed an
26 Moreover,
transient transfection and luciferase assays revealed th
27 of the mPNRC2 gene by deletion mutagenesis,
transient transfection and luciferase assays revealed th
28 reporter gene were generated and analyzed by
transient transfection and luciferase assays.
29 Results from
transient transfection and promoter reporter gene assays
30 Interestingly,
transient transfection and receptor mutational studies r
31 small molecule inducers in human cells with
transient transfection and stable chromosomal integratio
32 ression of each mutant in mammalian cells by
transient transfection and the identification of thermos
33 Transient transfections and electrophoretic mobility shi
34 Functional analyses by
transient transfections and Northern blot analyses sugge
35 o an increase in MIE gene expression in both
transient-transfection and infection assays.
36 In
transient-transfection and virus growth assays, the IR2P
37 H. virescens larvae, after TnBVank1 in vivo
transient transfection,
and in haemocytes of parasitised
38 ation of this site relieves P2 repression in
transient transfections,
and knockdown of endogenous YY1
39 to be functional in cell-free transcription,
transient-transfection,
and transgenic mouse assays.
40 Yet,
transient transfection approaches or the use of highly a
41 V-G)-pseudotyped LV preparations produced by
transient transfection are heavily contaminated with tub
42 ependent gene regulation, as demonstrated in
transient transfection assay and studies of endogenous g
43 Transient transfection assay showed that Osx activated 1
44 This was demonstrated using a
transient transfection assay system for GR transactivati
45 fficient for origin-dependent synthesis in a
transient-transfection assay, the carboxy-terminal segme
46 Transient transfection assays and Western blot analysis
47 Transient transfection assays and Western blotting analy
48 f the Epstein-Barr virus (EBV) BZLF1 gene in
transient transfection assays by directly binding its pr
49 and I90M each attenuated Sox9 expression in
transient transfection assays by twofold.
50 Transient transfection assays demonstrate that Fli-1 dri
51 Transient transfection assays demonstrated that WT1 can
52 As
transient transfection assays directly measure the promo
53 Subsequent
transient transfection assays in cultured primary prosta
54 To address this question, we used
transient transfection assays in human endothelial cells
55 s that regulate DeltaNp63, we have performed
transient transfection assays in keratinocytes with seve
56 Using
transient transfection assays in the Ishikawa endometria
57 Transient transfection assays indicate that the Pax5 bin
58 ith leptomycin B (LepB) or overexpression in
transient transfection assays inhibited endogenous Sost
59 Transient transfection assays of beta-cells with reporte
60 Transient transfection assays of the mutant protein demo
61 Finally,
transient transfection assays show that overexpression o
62 Transient transfection assays showed that Sp3-binding si
63 IGF-IR binding to IGF-IR promoter DNA and 2)
transient transfection assays showing transactivation of
64 toxicity, we find no significant evidence in
transient transfection assays that the HBV-miRNAs produc
65 In
transient transfection assays using 293T and HeLa cells,
66 with the endocytic pathway was evaluated in
transient transfection assays using IL-6-inducible STAT3
67 Transient transfection assays using promoter-reporter co
68 Conversely, in
transient transfection assays villin promoter activity r
69 -induced ERalpha immobilization/degradation,
transient transfection assays were performed using wild
70 Transient transfection assays with fragments of the beta
71 Because standard (short-term)
transient transfection assays with OC promoter-reporter
72 und that the level of Zp activity assayed by
transient transfection assays with reporter plasmids was
73 Transient transfection assays with RPGR promoter/lucifer
74 In
transient transfection assays, a PC1 C-terminal mutant l
75 r promoter activity in four cell lines using
transient transfection assays, and 25% of them showed tr
76 ' rapid amplification of cDNA ends analysis,
transient transfection assays, and DNase I hypersensitiv
77 ts was recapitulated in stable and long-term
transient transfection assays, in which the transfected
78 In
transient transfection assays, introduction of dominant-
79 Similarly, in
transient transfection assays, overexpression of HDAC3 d
80 In
transient transfection assays, p53 repressed PKD1 promot
81 In
transient transfection assays, p65 increased ZEB-1 promo
82 By
transient transfection assays, phosphorylation at S309,
83 In
transient transfection assays, SALL1 promoter activity w
84 In
transient transfection assays, TGF-beta stimulated the a
85 ring RNA, chromatin immunoprecipitation, and
transient transfection assays, we show that alpha-actini
86 early promoter activity at least 100-fold in
transient transfection assays.
87 e three sites abolished ZNF431 repression in
transient transfection assays.
88 .1 enhancer that mediates auto-activation in
transient transfection assays.
89 tested in the presence and absence of Rem in
transient transfection assays.
90 nd is capable of activating transcription in
transient transfection assays.
91 start site) could function as a promoter in
transient transfection assays.
92 strategy to identify silencers and EBs using
transient transfection assays.
93 nd both CTCF and Sp3 repress the promoter in
transient transfection assays.
94 n fluorescent protein was demonstrated using
transient transfection assays.
95 ene activity in a ligand-dependent manner in
transient transfection assays.
96 stable transfection and then with long-term
transient transfection assays.
97 promoter is functionally competent based on
transient transfection assays.
98 retinoid "X" receptor (RXR) or PPARgamma in
transient transfection assays.
99 et of these sites shows enhancer activity in
transient transfection assays.
100 HS10 acts as a coactivator of E3' in
transient transfection assays.
101 gulated expression of the mGrhl3 promoter in
transient transfection assays.
102 ansactivates viral and cellular promoters in
transient-transfection assays and is the ortholog of her
103 re expressed in human PBMCs, inhibit XMRV in
transient-transfection assays involving a single cycle o
104 Transient-transfection assays revealed that (i) the IR2P
105 Transient-transfection assays showed that the VZV IE62 l
106 Transient-transfection assays to determine whether mutat
107 f rhesus monkey rhadinovirus (RRV) following
transient-transfection assays under a variety of conditi
108 Transient-transfection assays which use a short CAV prom
109 Vpu and Nef exert anti-tetherin activity in
transient-transfection assays, mainly Vpu promotes RBF20
110 In
transient-transfection assays, we found that lpa2 overex
111 otein expression and productive infection in
transient-transfection assays.
112 can bind serum response factor (SRF) and by
transient transfection assessments showing that Tip60 an
113 ical potential of this approach, the low and
transient transfection associated with the large size of
114 We have developed a
transient transfection based method to enrich successful
115 Employing a
transient transfection-
based approach to rescue TSC2 fun
116 S-specific replication in HEK293 cells after
transient transfection,
being 17% as active as wild-type
117 A
transient-transfection ChIP assay demonstrated that the
118 GFR overexpression in cortical NG2+ cells by
transient transfection converted these cells to a migrat
119 Transient transfections demonstrate the inhibitory effec
120 SSAT1
transient transfection did not affect stable expression
121 eproducibility was observed between multiple
transient transfection events.
122 Fibroblasts that overexpress ST18 by
transient transfection exhibit significantly increased a
123 In a
transient transfection experiment, miR-298 directly boun
124 Transient transfection experiments and chromatin immunop
125 Transient transfection experiments establish that NOR1 t
126 Transient transfection experiments established that foll
127 Transient transfection experiments showed that DHA also
128 Transient transfection experiments showed that NMTS fusi
129 Transient transfection experiments showed that these pro
130 th the MMP9, MMP14, and MMP15 promoters, and
transient transfection experiments showed that these pro
131 Transient transfection experiments using NF-kappaB repor
132 Kinase assays and
transient transfection experiments were performed to stu
133 Kinase assay or
transient transfection experiments were performed to stu
134 Transient transfection experiments with 5'-deletion CRP
135 Transient transfection experiments with A549 lung adenoc
136 Transient transfection experiments with luciferase-aroma
137 Transient transfection experiments with RXRalpha promote
138 reporter molecules driven by TH promoter in
transient transfection experiments, data that provide ad
139 r significantly reduces receptor activity in
transient transfection experiments, however, and effecti
140 Furthermore, in
transient transfection experiments, Nkx2.5 colocalized o
141 Using
transient transfection experiments, we now show that ove
142 vo but does not ligand-activate PPARalpha in
transient transfection experiments.
143 ependent activity of the Ddelta2 promoter in
transient transfection experiments.
144 functionally repressing synthetic targets in
transient transfection experiments.
145 Data from
transient-transfection experiments show that the filamen
146 Transient-transfection experiments showed that full-leng
147 transactivation of the cyclin A promoter in
transient-transfection experiments.
148 mammalian cells and uses optimized chemical
transient transfection for introducing the essential gen
149 Transient transfection,
gel mobility shift assays, and c
150 Based on
transient transfection,
GSK3beta augments the transactiv
151 This recovery of promoter activity following
transient transfection had no effect on endogenous insul
152 In addition, genetic associations and
transient transfection identified PBF as a downstream ta
153 This was confirmed by
transient transfection in 293 cells: E7 could be transla
154 Using ChIP assay, siRNA treatment and
transient transfection in ARPE-19 cells in vitro, we dem
155 enovirus type 5 (Ad5) coinfection and during
transient transfection in either the presence or absence
156 sion of exogenous erbB3 via either stable or
transient transfection in erbB2-overexpressing, but not
157 FM2 was studied by confocal microscopy after
transient transfection in HEK293 cells.
158 rase chain reaction, DNA binding assays, and
transient transfections in colon cancer cell lines were
159 In
transient transfections in which AHR2-dependent transact
160 eport that expression of HCV core protein by
transient transfection increased cell proliferation, DNA
161 RIP2
transient transfection induced transcription of an NF-ka
162 ing either Hsp53 or Map53 were introduced by
transient transfection into the p53-null H1299 cell line
163 These results indicate that
transient transfection is a viable and efficient method
164 Transient transfection is accomplished overnight with ma
165 yed increased cyclin D1 promoter activity in
transient transfection luciferase reporter assays, and t
166 also enhanced the transcription of c-fos in
transient transfection luciferase reporter assays.
167 dian locomotor activity, Oil Red O staining,
transient transfection,
luciferase reporter assay, chrom
168 A
transient transfection,
luciferase-based, tissue culture
169 Recently,
transient transfection methods have been developed for t
170 R/Cas9 enhancer-deleted daughter cell lines,
transient transfection/
mutagenesis analyses, and transge
171 aborted replication could be restored by the
transient transfection of a CD81 expression plasmid.
172 Transient transfection of a constitutively active MRTF-A
173 Transient transfection of a p53-containing PAC clone int
174 essfully generated in nonpermissive cells by
transient transfection of a plasmid carrying both codon-
175 This effect was reversed by
transient transfection of a stable RGS4 mutant (HA-RGS4C
176 In this study, we found that
transient transfection of activated Akt triggered a 3-4-
177 Furthermore,
transient transfection of an activated RhoA construct pr
178 response was dependent on AhR expression as
transient transfection of an AhR expression plasmid into
179 Furthermore,
transient transfection of an APC segment encoding amino
180 Transient transfection of an E2F1 cDNA and 431 bp of the
181 en-3- one), and ZBTB16 was overexpressed via
transient transfection of an expression vector.
182 ce to invasive pathogens can be augmented by
transient transfection of antimicrobial mRNAs into epith
183 Transient transfection of artificial microRNA targeting
184 Transient transfection of AZIN1 SV2 complementary DNA in
185 enesis at a frequency of up to 15% following
transient transfection of both transformed and primary h
186 Transient transfection of cells with 5'-deletion constru
187 icantly correlated with CD38 expression, and
transient transfection of CLL cells with T-bet resulted
188 Transient transfection of COS-7 cells by PIAS1 and KLF5
189 However,
transient transfection of DCs with synthesized leader RN
190 On
transient transfection of diverse human and rodent cell
191 Transient transfection of dominant-negative forms of TLR
192 keratinocyte cell lines, HaCaT and NEB-1, by
transient transfection of each of the four K6a siRNAs.
193 We also found that
transient transfection of either a p53 short DNA sequenc
194 Transient transfection of either RARalpha or ERalpha exp
195 Transient transfection of full-length plectin cDNA conve
196 Transient transfection of G87R ZnT-2 resulted in endopla
197 spliced in C-100 compared with H1-177 cells;
transient transfection of gemin5 into C-100 cells restor
198 GANT61 induced gammaH2AX nuclear foci, while
transient transfection of Gli3R showed expression of Gli
199 Transient transfection of HEK293T cells with Wt (Wild ty
200 We have tested this model by
transient transfection of HeLa and HEK293 cells with a v
201 Transient transfection of HeLa cells with the mutants sh
202 Transient transfection of hepatic cell lines with NHERF1
203 Transient transfection of human colonic circular muscle
204 Transient transfection of human embryonic kidney (HEK) 2
205 Transient transfection of human embryonic kidney 293 cel
206 Transient transfection of KDR mutants into COS-7 cells s
207 Transient transfection of LNCaP and PC-3 cells with Atg5
208 In contrast,
transient transfection of MeCP2 expression plasmid into
209 to contribute to melanoma cell invasion, as
transient transfection of melanoma cells with CLDN1 incr
210 Transient transfection of miR-34a into glioma and medull
211 Transient transfection of miR-375 in oral cancer cells r
212 Conversely,
transient transfection of MM.1S cells with a previously
213 Transient transfection of MSCs with SIRT1-antisense olig
214 Transient transfection of murine mesangial cells with iN
215 Transient transfection of mutagenized NOTCH1 missense co
216 Transient transfection of neuronal GT1-7 cells with an N
217 Transient transfection of Ngb induced dose-dependent inc
218 Using
transient transfection of NIH/3T3 cells with wild type (
219 activities of E2 are typically evaluated by
transient transfection of nonreplicating E2-dependent re
220 Transient transfection of nonsecreted YFP-wild-type IGFB
221 Transient transfection of p53 into p53-null ovarian canc
222 erexpression or down-regulation of PGK using
transient transfection of pcDNA5-PGK or inducible shRNA
223 Overexpression of Nrf2 by
transient transfection of plasmid Nrf2 confers resistanc
224 measured using a dual luciferase assay after
transient transfection of reporter plasmids containing t
225 e cDNA constructs resulted in the successful
transient transfection of retinal cells.
226 r, further knockdown of IRPs accomplished by
transient transfection of small interfering RNA in stabl
227 Further treatment of T47D cells with
transient transfection of small interfering RNA targetin
228 in breast carcinoma cell invasion using the
transient transfection of small interfering RNA.
229 Consistently,
transient transfection of Snai1 into Smad4(-/-) keratino
230 The authors concluded that
transient transfection of SPIO-labeled cells with VLA-4
231 The effects of
transient transfection of SSc fibroblasts with FLI1 gene
232 Transient transfection of TCEAL7 inhibited the activatio
233 Transient transfection of tenocytes with ASO against Sir
234 Transient transfection of the Egr-1 gene into HeLa cells
235 Transient transfection of the ER-negative breast cancer
236 and Gli2 by GANT61 or genetic inhibition by
transient transfection of the Gli3 repressor (Gli3R) dow
237 eprogrammed cells demonstrated that a single
transient transfection of the miRNAs can direct a switch
238 Transient transfection of the Y1 and msc-1 cell lines wi
239 We demonstrated that
transient transfection of TLR2 signaling-negative regula
240 Transient transfection of various 5'-end deletion constr
241 Transient transfection of wild type p12 into U2OS cells
242 ution and stability of mature IGF-I protein,
transient transfections of cDNAs encoding murine IGF-IA,
243 Transient transfections of promoter reporter constructs
244 Transient transfections of these precise combinatorial c
245 ition, delivery of multiple miRNAs by either
transient transfection or lentivirus transduction increa
246 The expression of mNsp15 in trans by
transient transfection partially restored RNA synthesis
247 d in order to improve production yields in a
transient transfection production of a Sendai virus F/HN
248 In vitro,
transient transfection,
promoter/reporter constructs, an
249 or alpha (ERalpha)-mediated transcription in
transient transfection reporter assays, knockdown of ACT
250 tive promoters across eight cell lines using
transient transfection reporter assays.
251 L1B promoter region SNPs that were active in
transient transfection reporter gene assays.
252 Two
transient transfection-
reprogramming methods have been p
253 Using
transient transfection studies and deletion constructs o
254 Transient transfection studies and electrophoretic mobil
255 In
transient transfection studies in NIH3T3 cells, the LARG
256 Transient transfection studies of CHO cells with Nhs1-GF
257 Transient transfection studies of the human, mouse, and
258 Promoter deletion and
transient transfection studies showed that the estrogen
259 Transient transfection studies with wild-type and E3-def
260 In
transient transfection studies, the truncated AR is thre
261 "amyloid" box, we tested nine constructs in
transient transfection studies.
262 Transient-transfection studies revealed that UL116 is ef
263 titrated wt and deaminase-defective APO3G in
transient-transfection studies to achieve similar levels
264 tudies of the mutation using overexpression,
transient transfection systems.
265 With
transient transfection,
the expression of vIL-6 could be
266 On
transient transfection,
the protein encoded by exons 1-4
267 dies in which protein function is studied by
transient transfection,
three of the Survivin mutants fa
268 nit (caPKA), which was previously found upon
transient transfection to be sufficient for acquisition
269 Overexpression of TRB3 was induced by
transient transfection to determine the effects of TRB3
270 We used
transient transfection to test the ability of TFZ to act
271 We used
transient transfection to test the ability of ToxCast ch
272 u, PKC-epsilon, or PKC-theta was achieved by
transient transfection using small interfering RNAs.
273 this protocol, vector stocks are prepared by
transient transfection using standard cell culture media
274 When expressed in
transient transfections,
UXP complements the aberrant DB
275 the PI3K/Akt/mTORC1 axis, and CB(2) receptor
transient-transfection vector further supported that CB(
276 us in murine fibroblasts and 3T3 cells or by
transient transfection,
was monitored using Two Photon F
277 Using
transient transfection,
we overexpressed green fluoresce
278 Using
transient transfection,
we tested its ability to revert
279 Transient transfections were performed on human ARPE-19
280 With the goal of streamlining this process,
transient transfections were used to either (1) introduc
281 pressed expression from the ICP0 promoter in
transient transfection,
whereas Sp100A enhanced expressi
282 After
transient transfection,
wild-type MAPKAPK2 and HSP27 bot
283 In cultured tubular cells,
transient transfection with a miR-324-3p mimic reduced P
284 IF2Bepsilon expression was repressed through
transient transfection with a small interfering RNA dire
285 tability of the COX-2 gene was determined by
transient transfection with COX-2 reporter variants and
286 when 4-HNE is depleted in HLE B-3 cells by a
transient transfection with hGSTA4, Fas expression is su
287 Transient transfection with JAK2 short-interferring RNA
288 Enforced expression of miR-142-3p via
transient transfection with miR-142-3p mimic inhibited c
289 Transient transfection with miR-146b mimic and western b
290 fection of primary cells with HSV or CMV, or
transient transfection with naked plasmid DNA, HIRA re-l
291 Transient transfection with Nrf2 increased the transcrip
292 Transient transfection with promoter-linked luciferase r
293 tation system to rescue viral replication by
transient transfection with recombinant T3D muNS in whic
294 was measured by dual luciferase assay after
transient transfection with reporter plasmids.
295 e endogenous ligand(s) in CV-1 cells through
transient transfection with various cytochrome P450 isof
296 Transient transfection with vectors containing coding se
297 ifferent immortalized human cell lines using
transient transfections with a luciferase reporter assay
298 Transient transfections with COL1A1 promoter-reporter co
299 Transient transfections with dominant-negative and wild-
300 e quantitative RT-PCR, Western blotting, and
transient transfections with rat NP cells and lentiviral