ライフサイエンスコーパス: transportin

1 or Karyopherinbeta2 (Kapbeta2; also known as Transportin).

2 r importin-beta-like import factors, such as transportin.

3 A for human karyopherin beta2, also known as transportin.

4 zation signal or the M9 signal recognized by transportin.

5 lves its direct binding to importin beta and transportin.

6 hether mediated by importin alpha/beta or by transportin.

7 association with the nuclear import protein Transportin.

8 targets the 3' UTR of nuclear import factor transportin 1 (TNPO1) mRNA.

9 em to search for proteins that interact with transportin 1 (TRN1), the import receptor for shuttling

10 is recognized by the nuclear import receptor transportin 1 (Trn1; also called karyopherin-beta2) in a

11 argos (mCherry) for the importin alpha/beta, transportin 1, and transportin 3 import pathways and the

12 RNP H NLS interacts with the import receptor transportin 1.

13 mall GTPase Rab5, importin-beta (Kpnb1), and transportin-1 (Tnpo1).

14 Impbeta, but not transportin-1 (TRN1), alters the pore's permeability in

15 an bind cargo directly (e.g., importin-beta, transportin-1, transportin-3, importin-13) or through ad

16 nuclear localization signals, recognized by transportin-1.

17 bits a heat shock-sensitive interaction with transportin 2 (Trn2); the other involves two protein lig

18 mmunoprecipitation experiments revealed that transportin 2 binds directly to the ANS motif.

19 ACF binds to the protein carrier, transportin 2 in vivo, and colocalizes to the nucleus as

20 nuclear import pathway via IPO3 (importin 3, transportin 2) mediates stress-induced NEMO nuclear tran

21 We report that Kap beta2B (transportin-2) forms complexes with the mRNA export fact

22 Transportin 3 (TNPO3 or TRN-SR2) has been shown to be an

23 mplicated in viral nuclear import, including transportin 3 (TNPO3) and nucleoporin 358 (NUP358), in t

24 playing a role in nuclear import, including transportin 3 (TNPO3), a member of the importin-beta fam

25 One such factor, transportin SR2 (TRN-SR2)/transportin 3 (TNPO3), promotes infection by HIV-1 and s

26 Transportin 3 (Tnpo3, Transportin-SR2) is implicated in

27 ntrast, define a notably different integrase-transportin 3 binding hierarchy: FIV, HIV-1, and BIV > S

28 , is the dominant viral factor that dictates transportin 3 dependency during HIV-1 infection.

29 ical role for integrase binding in dictating transportin 3 dependency during retrovirus infection.

30 the importin alpha/beta, transportin 1, and transportin 3 import pathways and the Crm1-mediated expo

31 exception of FIV, display a requirement for transportin 3 in comparison to MLV and RSV, yielding an

32 reens have highlighted an important role for transportin 3 in human immunodeficiency virus type 1 (HI

33 to critically address the role of integrase-transportin 3 interactions in viral infection.

34 e failed to do so, suggesting that integrase-transportin 3 interactions might underscore active retro

35 Here we correlate infectivity defects in transportin 3 knockdown cells with in vitro protein bind

36 the genetic determinant of sensitization to transportin 3 knockdown to the HIV-1 capsid protein.

37 HIV-1 integrase interacted with recombinant transportin 3 protein under conditions whereby Moloney m

38 reens previously identified karyopherin beta transportin-3 (TNPO3) and NPC component nucleoporin 153

39 MD1E (DNAJB6), and more recently for LGMD1F (transportin-3).

40 irectly (e.g., importin-beta, transportin-1, transportin-3, importin-13) or through adaptor proteins

41 Transportin-SR2 (TRN-SR2, Transportin-3, TNPO3) is a cellular karyopherin implicat

42 ked from binding to kinetochores in vitro by transportin, a block reversible by M9M.

43 Diametrically opposed models for transportin action are as follows: 1) indirect action by

44 We investigated the transport properties of transportin and found that the carboxy-terminal region o

45 r define the nucleoporin subunit targets for transportin and importin beta and find them to be largel

46 he interplay of the two negative regulators, transportin and importin beta, along with the positive r

47 ryopherin beta1, termed karyopherin beta2 or transportin, and does not require a karyopherin alpha-li

48 , we tested the influence of Crm1, Imp beta, Transportin, and NTF2 on Ran sumoylation.

49 We show that transportin-and importin beta-initiate their regulation

50 Nevertheless, transportin appears unlikely to be the M9 export recepto

51 of the HEAT superfamily, importin beta, and transportin, as well as the export protein Cse1p, and th

52 from importin beta, and 2) direct action by transportin binding and inhibiting assembly factors.

53 fine amino acids in M9 that are critical for transportin binding in vivo.

54 Conversely, a Nup153 fragment containing the transportin binding site acted as a strong dominant-nega

55 onal randomization followed by selection for transportin binding to exhaustively define amino acids i

56 hnRNP A1 M9 element are shown to compete for transportin binding, they show no sequence homology, and

57 d, the resultant approximately 12-amino acid transportin-binding consensus sequence is also predictiv

58 Karyopherin-beta2 (transportin) binds a cognate import substrate and target

59 Karyopherin beta2 (Kapbeta2, transportin) binds the M9 sequence of human ribonucleopr

60 The structure of Kap beta 2 (also known as Transportin) bound to one of its substrates, the NLS of

61 ed by the import receptors importin beta and transportin, but not by the export receptor CRM1.

62 cient mutant of Ran, and was not observed if transportin carried cargo.

63 port pathways, mediated by importin beta and transportin, converge on a single nucleoporin, Nup153.

64 nd found that the carboxy-terminal region of transportin could, by itself, be imported into the nucle

65 A second importin-related protein, transportin, delivers a subset of heterogeneous nuclear

66 classical, importin alpha/beta-dependent and transportin-dependent nuclear import.

67 ct was reduced by RanGTP, a known ligand for transportin family members.

68 rity to several members of the importin beta/transportin family.

69 ude that the cell contains importin beta and transportin "global positioning system"or "GPS" pathways

70 Previously, transportin has been shown to mediate the nuclear import

71 only one protein, the nuclear import factor transportin, has been shown to bind M9 directly.

72 We have also isolated and sequenced a novel transportin homolog, transportin2, which may differ from

73 Transportin import and export were inhibited by low temp

74 NLS) present in Tap acts exclusively via the transportin import factor.

75 onstrate that in the importin alpha/beta and transportin import pathways, efficient in vitro transpor

76 d as a strong dominant-negative inhibitor of transportin import, with no effect on classical NLS impo

77 luble carriers known as karyopherins (Kaps), transportins, importins, or exportins.

78 Transportin is a 90 kDa protein, distantly related to im

79 mRNA export, it raises the possibility that transportin is a mediator of this process as well.

80 oapoptotic activity has a lower affinity for transportin, is displaced from it by RanGTP, and fails t

81 CC3 also inhibits nuclear translocation of transportin itself.

82 to loss of importin beta (Kap95p/Rsl1p) and transportin (Kap104p), conditional loss of Pse1p in a st

83 is recognized by the human karyopherin beta2/transportin (Kapbeta2) receptor.

84 e inhibitor of NLS import, with no effect on transportin-mediated import.

85 In this study, we show that transportin mediates the nuclear import of additional hn

86 Equally importantly, we find that transportin negatively regulates mitotic spindle assembl

87 find that the distantly related karyopherin, transportin, negatively regulates nuclear envelope fusio

88 s RanGTP can be shown to block M9 binding by transportin not only in vitro, but also in the nucleus i

89 ion sequence that displaces cargoes bound by transportin, or TLB, a mutant transportin that can bind

90 the nuclear import machinery utilized by the transportin pathway are conserved in evolution.

91 rotein nuclear import, the karyopherin beta2/transportin pathway is only the second to have a defined

92 e nuclear import receptors importin beta and transportin play a different role in mitosis: both act p

93 thway mediated by the recently characterized transportin protein.

94 port pathway, mediated by karyopherin beta2 (transportin), recently was described for mRNA-binding pr

95 Karyopherin beta2 or transportin recognizes a proline-tyrosine nuclear locali

96 The mechanism of transportin regulation was unknown.

97 erefore the first member of a novel class of transportin-specific NLSs that lack nuclear export signa

98 The SR protein import receptor, termed transportin-SR (TRN-SR), binds specifically and directly

99 Here we show that transportin-SR1 and transportin-SR2 are the alternativel

100 wo nuclear import receptors for SR proteins, transportin-SR1 and transportin-SR2.

101 One such factor, transportin SR2 (TRN-SR2)/transportin 3 (TNPO3), promote

102 The karyopherin transportin SR2 (TRN-SR2, TNPO3) is responsible for shut

103 Transportin-SR2 (Tnpo3, TRN-SR2), a human karyopherin en

104 Transportin-SR2 (TRN-SR2 and TNPO3) is a cellular cofact

105 Transportin-SR2 (TRN-SR2, Transportin-3, TNPO3) is a cel

106 Here we show that transportin-SR1 and transportin-SR2 are the alternatively spliced products o

107 were unable to identify an in vivo role for Transportin-SR2 in SLBP nuclear localization.

108 y spliced products of the same gene and that transportin-SR2 is the predominant transcript in most ce

109 Transportin 3 (Tnpo3, Transportin-SR2) is implicated in nuclear import of spli

110 cognition platform for the transport protein transportin-SR2.

111 th the import receptors Impalpha/Impbeta and Transportin-SR2.

112 ceptors for SR proteins, transportin-SR1 and transportin-SR2.

113 rgoes bound by transportin, or TLB, a mutant transportin that can bind cargo and RanGTP simultaneousl

114 e of a nuclear export receptor distinct from transportin that nevertheless shares a common protein-bi

115 associated with its nuclear import receptor (transportin), the cytoplasmic poly(A)-binding protein, a

116 n modulates the interaction of hnRNP A1 with transportin Trn1.

117 Recently, a 90-kD protein, transportin, was identified as the mediator of A1 nuclea

118 that other beta-like import factors, such as transportin, were unable to support Tat or Rev nuclear i

119 d nuclear import of Ran, whereas addition of transportin, which accumulates in the nucleus, enhanced

120 ed a specific M9-interacting protein, termed transportin, which binds to wild-type M9 but not to tran

121 which mediates classical NLS import, and for transportin, which mediates import of different nuclear

122 se processes by studying the import receptor transportin, which mediates the import of a group of abu

123 The interaction of transportin with Nup153 could be disrupted by a non-hydr

124 Saccharomyces cerevisiae transportin (yTRN; also known as YBR017c or Kap104p) has