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1 ical and have different affinities for their transposase.
2 (tnsABCDE) including an atypical heteromeric transposase.
3 e construct flanked by binding sites for the transposase.
4 lex Virus thymidine kinase (HSV-tk) with the transposase.
5 mpetent/integration defective (Exc(+)Int(-)) transposase.
6 rate mutants using the Himar1 transposon and transposase.
7 only the luciferase transposon and piggyBac transposase.
8 domains, they do not specify a fully active transposase.
9 gene is related to the Drosophila P-element transposase.
10 ure containing higher-order multimers of the transposase.
11 ss the Etv6-RUNX1 fusion and Sleeping Beauty transposase.
12 n sequence similarity of the element-encoded transposase.
13 two identical transposon ends and a dimer of transposase.
14 ect marker for the robust expression of SB10 transposase.
15 representing another approach to manipulate transposases.
16 those generated by retroviral integrases and transposases.
17 luding domesticated MULE and IS1595-like DDE transposases.
18 lation of ABC transport systems and putative transposases.
19 icted proteins homologous to RCR prokaryotic transposases.
20 chanism similar to retroviral integrases and transposases.
21 uyveromyces lactis hobo/Activator/Tam3 (hAT) transposase 1 (Kat1), operating at the fossil imprints o
22 oprecipitation, DNase I hypersensitivity and transposase-accessibility assays combined with high-thro
24 ychiatric disorders we applied the Assay for Transposase Accessible Chromatin followed by sequencing
26 DNaseI sequencing (DNase-seq) and Assay for Transposase Accessible Chromatin sequencing (ATAC-seq) p
27 re, the framework for applying the Assay for Transposase Accessible Sequencing (ATAC-seq) to biobanke
30 se (MNase) digestion and ATAC-seq (assay for transposase-accessible chromatin [ATAC] using sequencing
31 bility, as determined by ATAC-seq (assay for transposase-accessible chromatin [ATAC] with high-throug
32 equencing (ChIP-seq) combined with assay for transposase-accessible chromatin coupled to high-through
33 ble chromatin regions by using the Assay for Transposase-Accessible Chromatin from purified mouse car
34 atin accessibility profiling using assay for transposase-accessible chromatin sequencing (ATAC-seq) i
38 knockdown approaches, CRISPR-Cas9, assay for transposase-accessible chromatin sequencing, and chromat
39 ible genome of individual cells by assay for transposase-accessible chromatin using sequencing (ATAC-
41 onous neuronal activation using an assay for transposase-accessible chromatin using sequencing (ATAC-
42 n of the chromatin landscape (as assessed by transposase-accessible chromatin using sequencing (ATAC-
44 -seq) profiling combined with bulk assay for transposase-accessible chromatin with high-throughput se
45 ibitory cortical neurons using the Assay for Transposase-Accessible Chromatin with high-throughput se
47 We developed an allele-specific assay for transposase-accessible chromatin with high-throughput se
52 he route of an intact DNA strand through the transposase active site before second strand cleavage.
56 mice carrying the T2/Onc transposon and SB11 transposase alleles to allow transposon-mediated inserti
58 ted early during the interaction between the transposase and a potential target site, which may be ho
60 otein that regulates the activity of the MuA transposase and captures target DNA for transposition.
62 irectly at different ratios of transposon to transposase and found to be approximately comparable whi
63 study, we present a method that combines Tn5 transposase and molecular identifiers for the highly acc
64 lem by transposition immunity, involving MuA-transposase and MuB ATP-dependent DNA-binding protein.
65 ating type switching requires a domesticated transposase and occurs through a mechanism distinct from
66 ow that two ancestral RAG1 proteins, Transib transposase and purple sea urchin RAG1-like, have a late
67 a single plasmid construct that combines the transposase and the transpositioning transgene element t
69 to decrease post-transposition expression of transposase and to eliminate the cells that have residua
70 The use of PB in a plasmid containing both transposase and transposon greatly increased the probabi
71 ontal gene transfer between bacteria such as transposases and integrases) syntenic with ARGs were rar
73 0/IS605 family encode the smallest known DNA transposases and mobilize through single-stranded DNA tr
75 nt in most LTRs and require the DNA-binding, transposase, and dimerization domains of Abp1 to maintai
76 A and TnsB together form the heteromeric Tn7 transposase, and TnsD is a target-selecting protein that
77 e mobile DNAs, imprecision of integrases and transposases, and differential activity among identical
78 gone numerous rearrangements, contained many transposases, and might be less constrained by purifying
79 were capable of detecting 82% of the ISs and transposases annotated in GenBank with 80% sequence iden
80 d SPIN(ON) shows that no proteins other than transposase are essential for recombination, a property
81 stems, no helper plasmids are required since transposases are not expressed inside the host cells, th
83 ur analysis demonstrates that genes encoding transposases are the most prevalent genes in nature.
89 s ordered assembly process shepherds primary transposase binding to the inner 12DRs (where cleavage d
90 observed active site rearrangements when the transposase binds a metal ion in which it is inactive pr
96 the in vitro activities of the putative Cas1 transposase ('casposase') from Aciduliprofundum boonei.
97 re, we present two crystal structures of the transposase catalytic domain of Metnase revealing a dime
98 n-based systems that integrate transgenes by transposase-catalyzed "cut-and-paste" mechanism have eme
99 peats (TIRs) contain sequences essential for transposase cleavage and have been implicated in DNA rep
104 Intrabiliary instillation of the transposon-transposase complex was coupled with lobar bile duct lig
105 deciphered several inverted terminal repeat-transposase complexes that are intermediates during tran
107 and found that transposition declined after transposase concentrations became high enough for visibl
108 illuminates a molecular pathway involving a transposase-containing transcription factor that coopera
109 s (Fic; D-alanyl-D-alanine-carboxypeptidase; transposase) dated the divergence event at 300 million y
111 We conclude that Kat1 is a highly regulated transposase-derived endonuclease vital for sexual differ
112 molog FAR-RED IMPAIRED RESPONSE1 (FAR1), two transposase-derived transcription factors, are key compo
115 rucially, we find that each active site of a transposase dimer is responsible for two hydrolysis and
116 anscription factor SP1 fused to the piggyBac transposase directs insertion of the piggyBac transposon
117 r basis for the reduced affinity of the Mos1 transposase DNA-binding domain for the left IR as compar
120 chemical transfection or Lipofection of the transposase:DNA mixture, in contrast to other published
121 in Ac and Ds derivatives, suggesting that Ac transposase does not influence transcript initiation sit
123 ed the ssDNA-binding activity of the Metnase transposase domain and found that the catalytic domain b
124 red specific catalytic residues in the PGBD5 transposase domain as well as end-joining DNA repair and
126 Metnase (also called SETMAR) is a SET and transposase domain protein that promotes both DNA double
127 of Asn-610 with either Asp or Glu within the transposase domain significantly reduces ssDNA binding a
128 uires distinct aspartic acid residues in its transposase domain, and specific DNA sequences containin
131 ) arose from a chimeric fusion of the Hsmar1 transposase downstream of a protein methylase in anthrop
132 agenic T2Onc2 transposon via expression of a transposase driven by the keratin K5 promoter in a p53(+
133 s appear to have originated from prokaryotic transposases (e.g. TN7 and Mu) and combine a CDC6/ORC1-S
136 n vivo, leading to the production of the non-transposase-encoding mature mRNA isoform in Drosophila g
137 anied by alteration of their function from a transposase/endonuclease to a heterochromatin protein, d
139 arly, fusion of a fluorescent protein to the transposase enhanced the transposition activity, represe
142 9 protein fused to the amino-terminus of the transposase enzyme designed to target the hypoxanthine p
144 distantly related elements with heteromeric transposases exist with alternate targeting pathways tha
145 ved 5 (PGBD5) gene as encoding an active DNA transposase expressed in the majority of childhood solid
146 he genome size and inversely proportional to transposase expression and its affinity for the transpos
148 alternative strategies to achieve transient transposase expression, and engineered refinements in th
151 e integration; however, using transposon and transposase from separate vectors circumvented this.
154 cies, as well as in humans, but with loss of transposase function (except Schizosaccharomyces japonic
156 ransposase gene exists within a chimeric SET-transposase fusion protein referred to as Metnase or SET
158 escent protein (eGFP) was linked to the SB10 transposase gene as an indirect marker for the robust ex
159 he Hsmar1 transposon, the only intact Hsmar1 transposase gene exists within a chimeric SET-transposas
160 However, safety considerations regarding transposase gene insertions into host genomes have rarel
162 s1/2 (Rag1/2) recombinase has evolved from a transposase gene, demonstrating that TEs can be domestic
163 ulfurreducens strain DL-1, is truncated by a transposase gene, preventing flagellar biosynthesis.
166 y determined both ends of 87 ISs bearing 110 transposase genes in eight IS families and in a cluster
168 PCC 7120, widely studied, has 145 annotated transposase genes that are part of transposable elements
169 An unparalleled abundance of encoded transposases (>650) relative to genome size, together wi
170 ing their own replication and dissemination, transposases guarantee to thrive so long as nucleic acid
172 ilarity between RAG1 and the hairpin-forming transposases Hermes and Tn5 suggests the evolutionary co
177 here the induction of expression of multiple transposases in a Streptococcus mitis biofilm when the p
178 eaction and the mechanism of hAT and Transib transposases including the importance of the conserved W
179 that several point mutations in the mariner transposase increase their activities by disrupting the
180 nsposon greatly increased the probability of transposase integration; however, using transposon and t
183 a transgene flanked by binding sites for the transposase, into the cytoplasm of porcine zygotes.
184 e construct flanked by binding sites for the transposase, into the pronuclei of fertilized oocytes.
188 When purified recombinant Mos1 or Mboumar-9 transposase is co-transfected with transposon-containing
189 nsposition activity induced by the wild-type transposase is low but can be altered by modification of
190 r DNA dilution and compartmentalization, the transposase is removed, resolving the DNA into individua
193 ng the largest and most common among all DNA transposases, is the one whose members have been used fo
194 rmined the crystal structures of four ISDra2 transposase/IS end complexes; combined with in vivo acti
195 the structure and further show that the IS21 transposase, IstA, recognizes the IstB*DNA complex and p
196 atures suggest that, relative to IS200/IS605 transposases, it has evolved a different mechanism for t
197 In this study, we uncovered a domesticated transposase, Kluyveromyces lactis hobo/Activator/Tam3 (h
198 eta-catenin using sleeping beauty transposon/transposase leads to hepatocellular carcinoma (HCC) in m
199 rate that CRISPR/Cas9 combined with piggyBac transposase lineage labeling can produce unique models o
200 ore tested it in combination with a piggyBac transposase lineage labeling system to track the develop
201 relationship of its encoded protein to known transposases may have impeded the discovery of this grou
202 and arises from the multimeric state of the transposase, mediated by a competition for binding sites
205 e chromatin with visualization (ATAC-see), a transposase-mediated imaging technology that employs dir
209 osition in genetically engineered transposon-transposase mice induced cancers whose type (hematopoiet
211 mpared the preferences of two active mariner transposases, Mos1 and Mboumar-9, for their imperfect tr
214 e enhancer DNA segment is the site where the transposase MuA binds and makes bridging interactions wi
216 ed for the isolated C-terminal domain of the transposase MuA, which is not observed in the full-lengt
217 rt the structure of the Mu transpososome--Mu transposase (MuA) in complex with bacteriophage DNA ends
220 eins formed between the Drosophila P-element transposase N-terminal THAP DNA binding domain and the C
221 erated in different sister chromosomes after transposase nicks DNA near participating IS3 elements.
224 ee-dimensional structures of transpososomes (transposase-nucleic acid complexes) are available, and w
226 ed sensitive biochemical assays for the TnpA transposase of the Tn3-family transposon Tn4430 and used
228 ether with a suite of proteins that includes transposases, orchestrate a broad cascade of genome rear
229 Ac elements, these results give insight into transposase overproduction inhibition by demonstrating t
230 ne delivery system carries both the piggyBac transposase (pBt) expression cassette as well as the tra
231 ansfer activity dependent on codon-optimized transposase plasmid peaked at 100 ng with decreased colo
232 results were obtained with the domesticated transposase PogZ, another cellular interaction partner o
233 LPs) as carriers of the hyperactive piggyBac transposase protein (hyPBase), we demonstrate rates of D
234 We demonstrate lentiviral co-delivery of the transposase protein and vector RNA carrying the transpos
236 tion by demonstrating that the appearance of transposase protein structures and the end of active tra
237 genetic material encoding the gene-inserting transposase protein, raising concerns related to persist
239 d using combinations of both plasmid-DNA and transposase-protein relocalization to the target sequenc
244 in a lentivirus backbone containing PiggyBac transposase recognition elements together with fluoresce
247 ith guide RNA (gRNA), donor DNA and piggyBac transposase resulted in efficient, targeted genome editi
248 However, the 3'-end can be bypassed by the transposase, resulting in transduction of flanking seque
251 nding in both the predicted ancestral Hsmar1 transposase sequence as well as in the modern enzyme.
252 ow but can be altered by modification of the transposase sequence, including deletion, fusion, and su
253 functional relationships of hAT and hAT-like transposase sequences extracted from genome databases an
255 a method that exploits contiguity preserving transposase sequencing (CPT-seq) to facilitate the scaff
264 e, we describe modified versions of piggyBac transposase that have potentially wide-ranging applicati
266 binding sites for TnsB, the component of the transposase that specifically binds the ends and mediate
267 s indicate that human THAP9 is an active DNA transposase that, although "domesticated," still retains
269 TnsB, is from the large family of bacterial transposases, the second, TnsA, is related to endonuclea
270 n of these duplications is stimulated by IS3 transposase (Tnp) and plasmid transfer functions (TraI).
271 IS605 family, but in contrast to IS200/IS605 transposases, TnpA(REP) is a monomer, is auto-inhibited
272 scribed herein uses the hyperactive piggyBac transposase to insert a large transgene into the mouse g
273 multiple sites of interaction could allow a transposase to locate its transposon ends amidst a sea o
274 ansposon system using the SB100X hyperactive transposase to transduce human cord blood CD34(+) cells
275 ubstrate pair, Escherichia coli ClpX and MuA transposase, to address how these powerful enzymes recog
276 nthetic mRNA encoding the SB100X hyperactive transposase together with plasmid DNA carrying a transge
277 of a plasmid encoding the SB100X hyperactive transposase, together with a second plasmid carrying a t
278 nthetic mRNA encoding the SB100X hyperactive transposase, together with circular plasmid DNA carrying
279 g), green fluorescent protein (GFP), and the transposase (TPase) of maize (Zea mays) Activator major
280 such gene encodes the domesticated piggyBac transposase TPB6, required for heterochromatin-dependent
281 ng single-copy transposons at known loci and transposase transgenes exhibited coat color mosaicism, i
282 ilitated by one or more proteins, called the transposase, typically encoded by the mobile element its
283 xcised by a K248A excision(+)/integration(-) transposase variant are processed by hairpin resolution,
284 ers, fluorescent protein genes, and piggyBac transposase vectors to demonstrate that this can be a re
285 ector in new genomic locations when piggyBac transposase was provided in trans from a second integrat
288 ures of wild-type and catalytically inactive transposases, we show that all the catalytic steps of tr
289 y express either piggyBac or Sleeping Beauty transposase were generated by standard zygote injection
292 over, we identified a critical region in the transposase where the net charge of the amino acids seem
293 (DDN) differs from the DDD motif of related transposases, which may be important for its role as a D
294 lso be a useful intermediate in generating a transposase whose integration activity could be rescued
295 cular group of DNA transposons that encode a transposase with a DD(E/D) catalytic domain that is topo
296 We found that the FKBP-DD confers the PB transposase with a higher transposition activity and bet
299 A hyperstable complex of the tetrameric MuA transposase with recombined DNA must be remodeled to all
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