ライフサイエンスコーパス: transposition

1 ase in copy number due to periodic bursts of transposition.

2 reakage and rejoining reactions required for transposition.

3 ed in the C2C12 myoblasts that had undergone transposition.

4 h all of the domains required for autonomous transposition.

5 us COM1 strain and of in vitro Mariner-based transposition.

6 imes a selected insertion site for efficient transposition.

7 boumar-9 may be a general feature of mariner transposition.

8 ature target site duplication of mariner/Tc1 transposition.

9 DNA-protein binding, DNA replication and DNA transposition.

10 sase complexes that are intermediates during transposition.

11 our-muscle tenotomy and reattachment without transposition.

12 uld represent intermediate structures before transposition.

13 involved in the TnsB-TnsC interaction during transposition.

14 al sweep was shown to have involved a 700-kb transposition.

15 and results in a bent DNA favored by MuA for transposition.

16 ange of formyl-substituted rings with alkene transposition.

17 d the characterization of key steps in Muta1 transposition.

18 d regio- and stereospecific substitutive 1,3-transposition.

19 must hijack the machinery required for their transposition.

20 recleaved ends mimicking the initial step of transposition.

21 Dsg requires the autonomous element Ac for transposition.

22 n reactions, such as V(D)J recombination and transposition.

23 tive malonate alkylation followed by C- to N-transposition.

24 then a Curtius rearrangement to provide the transposition.

25 erminal inverted repeat also influence Muta1 transposition.

26 nt locus indicate the origin and target of a transposition.

27 in 50+/-5% GFP-expressing cells after stable transposition.

28 facts are often mistaken for genuine somatic transposition.

29 al tool to unravel the mechanism of Helitron transposition.

30 Remarkably, 25% of these were transpositions.

31 erage, and is particularly suited to mapping transpositions.

32 triction can be bypassed through alternative transposition, a transposition reaction that utilizes th

33 This report describes potent DNA transposition achieved by direct delivery of transposase

34 ns provided a fine source for analyzing MITE transposition activities and their impacts on genome evo

35 As the first study concentrated in the transposition activities of MITEs among different ecotyp

36 -DD confers the PB transposase with a higher transposition activity and better dynamic range than can

37 in plants and are well known for their high transposition activity as well as their ability to dupli

38 ion yet a comprehensive assessment of recent transposition activity at the species level is lacking f

39 The apparent conservation of DNA transposition activity by PGBD5 suggests that genomic re

40 The transposition activity induced by the wild-type transpos

41 analysis of a series of TnsE mutants relates transposition activity to DNA binding stability.

42 transposases were evaluated for expression, transposition activity, chromatin immunoprecipitation at

43 cent protein to the transposase enhanced the transposition activity, representing another approach to

44 p22/p18 strongly inhibits transposition and can be considered an element-encoded r

45 structure that delivers the observed allylic transposition and completes a novel P(III)/P(V) redox ca

46 Besides creating mutations via transposition and contributing to genome size, TEs play

47 aracterized mechanism for provoking internal transposition and deletion events that serve as a condui

48 Interestingly, transposition and deletion rates remain constant across

49 body diversification by interchromosomal DNA transposition and demonstrate the existence of conserved

50 findings suggest the existence of RNA-guided transposition and fit the guns-for-hire concept whereby

51 observed, but instead we found active (retro)transposition and gene family expansion, especially in p

52 In addition, yeast transposition and in vitro assays demonstrated that the

53 ariation between the amount of DNA gained by transposition and lost by deletion during mammal and avi

54 , regioselective O-exchange, Stork-Danheiser transposition and O-methylation reactions were observed.

55 Combinatorial 96-plex indexing at both the transposition and PCR stage enables the construction of

56 g a new role for the WVPHEL motif in mariner transposition and providing a molecular explanation for

57 poson methylation and collaborate to repress transposition and regulate the methylation and expressio

58 ffecting TEs independently of their modes of transposition and regulation.

59 Thus, understanding factors that limit transposition and retroviral replication is fundamentall

60 ation, homologous recombination, DNA repair, transposition and RNA interference.

61 SB-mediated genetic transposition and stimulation resulted in 2,200- to 2,50

62 mediated insertion pathway distinct from DNA transposition and trans-V(D)J recombination that destabi

63 /2 can also induce genomic DNA insertions by transposition and trans-V(D)J recombination, but only fe

64 human primates reveals a 746-kbp duplicative transposition and two separate inversion events that aro

65 against the reference sequence revealed 564 transpositions and 47 inversions comprising approximatel

66 ent of large-scale rearrangements, including transpositions and inversions.

67 ts provide mechanistic insight into Helitron transposition, and its impact on diversification of gene

68 transposon excision, host DNA recombination, transposition, and low-efficiency TIR rejoining using re

69 ts of Tn7 and retroviral and retrotransposon transposition, and may hint at a common gap-repair mecha

70 (MITEs) are preferred substrates of mariner transposition, and this process is associated with the e

71 ase protein structures and the end of active transposition are simultaneous, an effect with implicati

72 rich mobilome can have and the importance of transposition as a recurrent generator of large-effect a

73 few Ty1 elements are present and inhibiting transposition as copy number increases.

74 ication and, to a lesser extent, single gene transposition as having played roles in the evolution of

75 ptase inhibitor 3TC resulted in decreased TE transposition as well as increased life span in TE-sensi

76 Studying the dynamics of transposition at the population level can provide crucia

77 Most of the present knowledge on the transposition behavior of this superfamily comes from st

78 n interaction with TnsB is defective for Tn7 transposition both in vitro and in vivo.

79 precisely, we validated 83% of 44 predicted transposition breakpoints by polymerase chain reaction.

80 Thus, each transposition burst generates a novel progeny population

81 cumulation in plant populations experiencing transposition bursts accompanied by high diversity of ch

82 anisms, of which the first prevents multiple transposition bursts in a given somatic cell lineage tha

83 Here, we studied transposition bursts of a heat-activated retrotransposon

84 he amount of DNA gained via lineage-specific transposition, but that DNA loss counteracted this expan

85 Radical transposition by C(sp(3) )-(sp(3) ) and C(sp(3) )-H bond

86 lostery that normally serves to downregulate transposition by slowing synapsis of the transposon ends

87 when coexpressed with RAG2 and that in vitro transposition by Transib transposase is stimulated by RA

88 The timing of past transposition can be estimated by quantifying the accumu

89 citing reactivity patterns like 1,3-carbonyl transpositions, carbene transfer reactions, cascade annu

90 DNA transposition catalyzed by PGBD5 in human cells occurs g

91 In Drosophila, P-element transposition causes mutagenesis and genome instability

92 study nicely illustrates how the presence of transposition-competitive variants can deeply impair TE

93 as tetralogy of Fallot, truncus arteriosus, transposition complexes, endocardial cushion defects, an

94 DNA transposition contributes to genomic plasticity.

95 iogenesis leading to changes in the level of transposition control.

96 pect natural assumptions of invariance under transposition, convexity, and contractivity under quantu

97 SINE_Scan integrates hallmark of SINE transposition, copy number and structural signals to ide

98 These results show how alternative transposition coupled with DNA replication and repair ca

99 e haplotyping based on contiguity-preserving transposition (CPT-seq) and combinatorial indexing.

100 psis that old members enter into replication/transposition cycles through high rates of intra-family

101 ability of the transposon DNA and found that transposition declined after transposase concentrations

102 eir imperfect transposon IRs in each step of transposition: DNA binding, DNA cleavage, and DNA strand

103 Our results suggest that alternative transposition during S phase can induce re-replication o

104 Here, we describe the transposition dynamics of cut-and-paste mariner elements

105 Finally, transposition efficiency is also influenced by the eleme

106 e fold-induction achieved, (ii) the absolute transposition efficiency of the activated construct and

107 Transposition efficiency was first tested using an enhan

108 e among transposable elements in employing a transposition enhancer.

109 ed carbonaria haplotypes indicates that this transposition event occurred around 1819, consistent wit

110 s neither shortened nor lengthened with each transposition event.

111 ion and pinpoint junctions of high frequency transposition events at nucleotide resolution.

112 d lineage-specific clusters that derive from transposition events for the regulators of floral develo

113 cts both the source and destination of novel transposition events in re-sequenced genomes.

114 ples; accurately estimate the frequencies of transposition events in the population and pinpoint junc

115 ions, we found that frameshift mutations and transposition events increased in the progeny of older p

116 E-Tracker to provide a comprehensive view of transposition events induced by loss of DNA methylation

117 s the globe, we identify thousands of recent transposition events involving half of the 326 TE famili

118 ectious particles that can lead to heritable transposition events.

119 This requires that each substrate transposition exposes a catalyst activation mode (AM) to

120 we propose a vector design to decrease post-transposition expression of transposase and to eliminate

121 ccess rate and low complication rate, muscle transposition flap repairs remain an attractive surgical

122 t combinations required for large changes in transposition frequency and targeting stabilized this in

123 A new study provides evidence that gene transposition from sex chromosomes to autosomes is a con

124 ore, we identified widespread natural AtMu1c transposition from the analysis of over 200 accessions,

125 loss emerges as an additional driver of gene transposition from the sex chromosomes, a phenomenon tho

126 or these species were the result of a direct transposition from vertebrates, despite deep evolutionar

127 Helraiser transposition generates covalently closed circular inter

128 Superior rectus transposition has been popularized for the treatment of

129 ulation, and genomic impact of cut-and-paste transposition in a natural mammalian host.

130 the transposon sequence, allowing robust DNA transposition in a variety of cell types.

131 nowledge, the first demonstrations of intron transposition in any organism.

132 corresponding age-associated increase in TE transposition in fly fat body cells that was delayed by

133 , can induce stereotypical cut-and-paste DNA transposition in human cells.

134 Somatic transposition in mammals and insects could increase cell

135 To understand the impact of transposition in somatic cells it is essential to reliab

136 red for efficient transposon integration and transposition in vitro.

137 lement from Aedes aegypti and its successful transposition in yeast facilitated the characterization

138 However, certain transposition-induced gain-of-function or regulatory mut

139 Our aim was to exploit two Ds transposition-induced mutant lines of Arabidopsis thalia

140 The analysis of 278 transposition-induced prostate, breast and skin tumors d

141 of species; however, it is unclear how much transposition-induced variation exists at a smaller scal

142 TnsC is the key regulator of transposition, interacting with both the TnsAB transposa

143 facilitates horizontal transfer by directing transposition into actively replicating DNA with the ele

144 TnsD directs transposition into attTn7, while TnsE encourages horizon

145 es a target-site-selecting protein to target transposition into certain forms of DNA replication.

146 marker to follow repair, we find that after transposition into the chromosome, the unrepaired Mu is

147 After transposition into the Escherichia coli chromosome, the

148 Furthermore, transposition into the genome ensures the longevity of c

149 Transposition involves five transposon-encoded genes (tn

150 A new procedure, the inferior rectus transposition (IRT), may be similarly beneficial for pat

151 Mariner transposition is a complex reaction that involves three

152 Our findings show that piggyBac transposition is a more efficient approach than the clas

153 As are self-propagating, and their continued transposition is a source of both heritable structural v

154 tial analysis of new insertions suggest that transposition is biased to proximal insertions, and the

155 The data suggest that transposition is controlled at an early stage of transpo

156 We show that the efficiency of Mboumar-9 transposition is improved almost 4-fold by changing the

157 aryotes, but experimental evidence for their transposition is lacking in the absence of an isolated a

158 We conclude that somatic transposition is less prevalent in Drosophila than previ

159 s of the maize (Zea mays) Mu elements, whose transposition is mediated by the autonomous Mutator-Don

160 For both Mos1 and Mboumar-9, in vitro DNA transposition is most efficient when the preferred IR se

161 for many experiments it is important that PB transposition is tightly controlled.

162 Tn5 transposition is used to modify DNA with adaptor and ind

163 posase element from the polyA sequence after transposition leads to its deactivation.

164 ovalently joined to target DNA, portrays the transposition machinery after DNA integration.

165 autonomous mariner elements by hijacking the transposition machinery produced by active mariner, whic

166 es the foundation for further studies of the transposition mechanism of MULEs.

167 d importance as genetic tools in plants, the transposition mechanism of the MULE superfamily was prev

168 r intermediates, suggestive of a replicative transposition mechanism, which provides a powerful means

169 and biological significance as well as their transposition mechanisms are poorly understood.

170 es have autosomal paralogs, so autosome-to-Y transposition must be the main source of Drosophila Y-li

171 Transposition mutagenesis is a powerful tool to identify

172 This transposition mutagenesis strategy will greatly facilita

173 ses, we show that all the catalytic steps of transposition occur within the context of a dimeric tran

174 We found that transposition occurs among memory-relevant neurons in th

175 abeling experiments disclose that the oxygen transposition occurs from the carboxylate group to the t

176 cation mechanism, it was recently shown that transposition of a bat Helitron generates covalently clo

177 vealed that the Abph2 mutation was caused by transposition of a glutaredoxin gene, MALE STERILE CONVE

178 ron gain events characterized by the perfect transposition of a reporter intron into the yeast genes

179 ividual-based simulations that allow for the transposition of a single genetic locus from one positio

180 The direct metal-catalyzed [1,3]-transposition of allylic alcohols and allylic silyl ethe

181 Although [1,3]-transposition of allylic alcohols has been known since t

182 se of epoxides as electrophilic traps in the transposition of allylic alcohols.

183 t full details of a method for 1,3-reductive transposition of alpha-alkoxy-alpha,beta-unsaturated hyd

184 Because these mutational events involved the transposition of an insertion sequence into a narrow win

185 e we describe an evolutionary event in which transposition of an IS element has a direct impact on vi

186 The transposition of bacteriophage Mu serves as a model syst

187 The high-frequency transposition of both TcBuster and SPIN(ON) suggests tha

188 in to be clarified but likely ensue from the transposition of center of mass of the ion within its ge

189 e RCM followed by a ring-contractive allylic transposition of cyclic silyl ethers was incorporated fo

190 With the recent transposition of Directive 2010/53/EU into the transplan

191 rmation categories, eg, neural tube defects, transposition of great vessels, ventricular septal defec

192 he mobilisation of bla CMY-42 was due to the transposition of ISEcp1 by misidentifying its right-end

193 (3,18)-connected MOF followed by deliberate transposition of its topology to a predesigned second MO

194 t yeast harbors all the host factors for the transposition of MULEs.

195 ly, we detected both the deletion and/or the transposition of multicopy TEs associated with 2b-induce

196 ral analysis in RSPH1-mutated cilia revealed transposition of peripheral outer microtubules into the

197 We report that a regioselective reductive transposition of primary allylic bromides is catalyzed b

198 Our data suggest that transposition of selfish DNA, low effective population s

199 uencing (ATAC-seq), based on direct in vitro transposition of sequencing adaptors into native chromat

200 Ptch mutations permit transposition of SMO to the primary cilium followed by e

201 ocess features regio- and stereospecific 1,3-transposition of the allyl fragment enabled by an aromat

202 CHD, such as single ventricle (22.8%) and d-transposition of the great arteries (22.1%).

203 sis and management of congenitally corrected transposition of the great arteries (ccTGA).

204 s" in the management of patients with D-loop transposition of the great arteries (D-TGA) in the curre

205 that of 15 fetuses with major heart defects: transposition of the great arteries (n=7), coarctation o

206 Patients with transposition of the great arteries (TGA) and systemic r

207 tle has been published regarding surgery for transposition of the great arteries (TGA) in the develop

208 haemodynamic effects of exercise training in transposition of the great arteries (TGA) patients with

209 n, the Mustard or Senning operation, for the transposition of the great arteries (TGA) was introduced

210 astic function of the aorta in patients with transposition of the great arteries after arterial switc

211 operation and 2 by aortopulmonary shunts: d-transposition of the great arteries after Mustard/Sennin

212 Among 153 patients (67% male), 96 had transposition of the great arteries and 57 had single ve

213 A total of 153 patients with transposition of the great arteries and single ventricle

214 ved for the CNVs identified in patients with transposition of the great arteries and tetralogy of Fal

215 ular septal defect, tetralogy of Fallot, and transposition of the great arteries decreased to zero fo

216 diagnosis of single ventricle physiology and transposition of the great arteries demonstrate less pre

217 with tetralogy of Fallot and 7 patients with transposition of the great arteries demonstrated compres

218 Only infants with transposition of the great arteries had smaller HC relat

219 e novo CNVs were identified in patients with transposition of the great arteries in contrast to coarc

220 , the 22-year survival rate of patients with transposition of the great arteries operated on in 1953

221 resonance predicts outcomes in patients with transposition of the great arteries post atrial redirect

222 Transposition of the great arteries was associated with

223 , including 76 coarctation of the aorta, 159 transposition of the great arteries, and 81 tetralogy of

224 TPVR in patients with congenitally corrected transposition of the great arteries, subpulmonary left v

225 Other single-ventricle defects, transposition of the great arteries, tetralogy of Fallot

226 se clinical outcome especially arrhythmia in transposition of the great arteries, thus LGE cardiovasc

227 to double-outlet right ventricle and dextro-transposition of the great arteries.

228 ally in patients with tetralogy of Fallot or transposition of the great arteries.

229 were men, and 28% had congenitally corrected transposition of the great arteries.

230 n; 12 for Eisenmenger; 9 for Fontan; 9 for D-transposition of the great arteries; and 8 for tetralogy

231 ty and aortic root dilatation are present in transposition of the great artery patients post ASO and

232 patients were younger, with higher rates of transposition of the great vessels and common ventricle

233 Transposition of the great vessels and common ventricle

234 efect, tetralogy of Fallot, Ebstein anomaly, transposition of the great vessels, and common ventricle

235 tandem annulation is proposed to proceed via transposition of the hydroxy group resulting from the in

236 iphenols, results in an asymmetric reductive transposition of the in situ generated allylic diazene.

237 rce of genotoxic effects due to uncontrolled transposition of the integrated transgene from one chrom

238 yPB) transposase was then deployed to enable transposition of the large-size PB transposon (17 kb) en

239 ISApl1 was presumably involved in the transposition of the mcr-1 cassette and then was lost.

240 d nerve palsy who underwent adjustable nasal transposition of the split LR muscle between 2010 and 20

241 Adjustable nasal transposition of the split LR muscle can achieve excelle

242 Adjustable nasal transposition of the split LR muscle.

243 erties of rAAV with stable piggyBac-mediated transposition of the transgene into the hepatocyte genom

244 The transposition of this technique to the gas phase is appe

245 autonomous retrotransposons and very recent transpositions of a TRIM element in Oryza sativa.

246 izes recently developed regioselective [1,3]-transpositions of allylic alcohols and silyl ethers and

247 ctive in vitro for all the chemical steps of transposition, only octamers are active in vivo.

248 or acquired by horizontal gene transfer via transposition or homologous recombination.

249 These gene transpositions originated from either the X or Y chromos

250 tein Trap Consortium generated, via piggyBac transposition, over 600 novel YFP-trap proteins tagging

251 The transposition pathway resembles that of the V(D)J recomb

252 DNA transposition plays key roles in genome diversity, patho

253 e silencing of active elements and influence transposition potential, siRNA targeting levels, and ult

254 operative hypertropia or intorsion requiring transposition procedures.

255 Target capture is a key step in the transposition process, because it contributes to the sel

256 improve our understanding of their origins, transposition processes, regulatory mechanisms, and biol

257 We demonstrate that the final transposition product, which is a DNA-bound tetramer of

258 stly to unlinked sites and can form circular transposition products.

259 site attTn7, is mediated by four Tn7-encoded transposition proteins: TnsA, TnsB, TnsC, and TnsD.

260 ly invasive and characterized by an elevated transposition rate, confirming their capacity to spread

261 efficient, regioselective gold-catalyzed 1,3-transposition reaction of ynones and diynones has been d

262 A small-scale transposition reaction routinely generates several hundr

263 ypassed through alternative transposition, a transposition reaction that utilizes the termini of two

264 tructures of these enzymes at the end of the transposition reaction, including Mu, exhibit significan

265 e ramifications of target DNA bending on the transposition reaction.

266 em aldol cyclizations, and one-pot reduction/transposition reactions allowing a rapid (7 linear steps

267 Allylic alcohols undergo transposition reactions in the presence of Re2 O7 whereb

268 Transposition requires the assembly of a nucleoprotein c

269 RNA-cDNA molecule is required for mutagenic transposition, revealing a unique mechanism of DNA hyper

270 e method that we call "contiguity preserving transposition" sequencing on beads (CPTv2-seq) is transp

271 Rectus muscle transposition surgery to either treat pattern strabismus

272 ession systems and the PiggyBac-mediated DNA transposition system in avian embryos.

273 Here we describe a conditional piggyBac transposition system in mice and report the discovery of

274 cluding Mu, exhibit significant bends in the transposition target site DNA.

275 t, structural and mechanistic information on transposition targeting and regulation is limited.

276 ses to the environment are the most frequent transposition targets observed.

277 ovel array of green paddles in a traditional transposition task.

278 the 3'-end of the transposon functions as a transposition terminator.

279 otein (hyPBase), we demonstrate rates of DNA transposition that are comparable with the efficiency of

280 predicted to change during the final step of transposition, this recognition strategy suggests how AA

281 view, 5 in whom torsion developed because of transposition to address pattern strabismus and 3 in who

282 Rectus muscle transposition to address pattern strabismus may cause to

283 ss pattern strabismus may cause torsion, and transposition to address torsion may cause pattern strab

284 3 in whom pattern strabismus developed after transposition to address torsion.

285 ology, was subjected to a reductive carbonyl transposition to afford 8-(4-methoxyphenyl)perinaphthano

286 Rescue of Y-linked gene loss through transposition to autosomes has previously been reported

287 For two genes--EIF1AX/Y and RPS4X/Y--transposition to autosomes occurred independently in thr

288 equence-independent structures maintained by transposition to chromosome ends of three specialized re

289 ation of saturated target sites by promoting transposition to different target sites.

290 The approach utilizes in vitro transposition to generate 5 and 3 fragment sub-libraries

291 species, many Y-linked genes were rescued by transposition to new genomic locations, but until our wo

292 ich renders TSPX to be stimulatory while its transposition to the C-terminus of TSPY results in an in

293 We demonstrated user-defined directed transposition to the CCR5 genomic safe harbor and isolat

294 struct that combines the transposase and the transpositioning transgene element to share a single pol

295 During cut-and-paste mariner/Tc1 transposition, transposon DNA is cut precisely at its ju

296 uses a cocktail of labelling, targeting and transposition vectors that enables targeting of specific

297 Using transgenes and transpositions, we show that most endogenous X-linked ge

298 Transpositions were also detected, revealing replicon pr

299 In contrast to mariner transposition, where a topological filter suppresses sin

300 s (inversions, reciprocal translocations and transpositions), whereas S. cerevisiae accumulates unbal